CA2722766A1 - Methods for producing enucleated erythroid cells derived from pluripotent stem cells - Google Patents

Methods for producing enucleated erythroid cells derived from pluripotent stem cells Download PDF

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Publication number
CA2722766A1
CA2722766A1 CA2722766A CA2722766A CA2722766A1 CA 2722766 A1 CA2722766 A1 CA 2722766A1 CA 2722766 A CA2722766 A CA 2722766A CA 2722766 A CA2722766 A CA 2722766A CA 2722766 A1 CA2722766 A1 CA 2722766A1
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Prior art keywords
cells
cell
pluripotent stem
human
culture
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Abandoned
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CA2722766A
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English (en)
French (fr)
Inventor
Robert Lanza
Shi-Jiang Lu
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Astellas Institute for Regenerative Medicine
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Advanced Cell Technology Inc
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Application filed by Advanced Cell Technology Inc filed Critical Advanced Cell Technology Inc
Priority to CA3150872A priority Critical patent/CA3150872A1/en
Publication of CA2722766A1 publication Critical patent/CA2722766A1/en
Abandoned legal-status Critical Current

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    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0641—Erythrocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0644—Platelets; Megakaryocytes
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/069—Vascular Endothelial cells
    • C12N5/0692—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/60—Transcription factors
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1352—Mesenchymal stem cells
    • C12N2502/1358—Bone marrow mesenchymal stem cells (BM-MSC)
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    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/78—Cellulose

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Biomedical Technology (AREA)
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  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Vascular Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Diabetes (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Rheumatology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2722766A 2008-05-06 2009-05-06 Methods for producing enucleated erythroid cells derived from pluripotent stem cells Abandoned CA2722766A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3150872A CA3150872A1 (en) 2008-05-06 2009-05-06 Methods for producing enucleated erythroid cells derived from pluripotent stem cells

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US12680308P 2008-05-06 2008-05-06
US61/126,803 2008-05-06
US18949108P 2008-08-19 2008-08-19
US61/189,491 2008-08-19
US19028208P 2008-08-26 2008-08-26
US61/190,282 2008-08-26
PCT/US2009/043050 WO2009137629A2 (en) 2008-05-06 2009-05-06 Methods for producing enucleated erythroid cells derived from pluripotent stem cells

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3150872A Division CA3150872A1 (en) 2008-05-06 2009-05-06 Methods for producing enucleated erythroid cells derived from pluripotent stem cells

Publications (1)

Publication Number Publication Date
CA2722766A1 true CA2722766A1 (en) 2009-11-12

Family

ID=41265390

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2722766A Abandoned CA2722766A1 (en) 2008-05-06 2009-05-06 Methods for producing enucleated erythroid cells derived from pluripotent stem cells
CA3150872A Pending CA3150872A1 (en) 2008-05-06 2009-05-06 Methods for producing enucleated erythroid cells derived from pluripotent stem cells

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3150872A Pending CA3150872A1 (en) 2008-05-06 2009-05-06 Methods for producing enucleated erythroid cells derived from pluripotent stem cells

Country Status (10)

Country Link
US (4) US20110086424A1 (enExample)
EP (2) EP2288690B1 (enExample)
JP (4) JP5748654B2 (enExample)
KR (4) KR101803562B1 (enExample)
CN (3) CN102083960B (enExample)
AU (1) AU2009244236B2 (enExample)
BR (1) BRPI0912517A2 (enExample)
CA (2) CA2722766A1 (enExample)
MX (1) MX2010012089A (enExample)
WO (1) WO2009137629A2 (enExample)

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JP5748654B2 (ja) 2008-05-06 2015-07-15 オカタ セラピューティクス， インコーポレイテッド 多能性幹細胞に由来する除核赤血球細胞を産生するための方法
CA2722693C (en) * 2008-05-06 2023-03-21 Advanced Cell Technology, Inc. Hemangio colony forming cells and non-engrafting hemangio cells
EP2285832B1 (en) 2008-05-16 2020-08-26 Taiga Biotechnologies, Inc. Antibodies and processes for preparing the same
ES2681478T3 (es) 2008-08-28 2018-09-13 Taiga Biotechnologies, Inc. Moduladores de MYC, métodos de uso de los mismos y métodos para identificar agentes que modulan MYC
US8703487B2 (en) 2009-12-01 2014-04-22 The Regents Of The University Of California Compositions and methods for making and using bone marrow mesenchymal stem cells and erythroid progenitor cells
US9988603B2 (en) * 2009-12-04 2018-06-05 Stem Cell & Regenerative Medicine International Large scale generation of functional megakaryocytes and platelets from human embryonic stem cells under stromal-free conditions
KR20210120136A (ko) * 2009-12-04 2021-10-06 아스텔라스 인스티튜트 포 리제너러티브 메디슨 인간 배아줄기세포 유래된 혈액모세포로부터 자연 살해 세포 및 수지상 세포를 생성하는 방법
BR112012020958A2 (pt) * 2010-02-22 2015-09-15 Univ Paris Curie "meio de cultura para a criação e/ou diferenciação de células da linhagem hemapo
TWI812867B (zh) * 2011-11-30 2023-08-21 安斯泰來再生醫藥協會 利用血管母細胞產生間葉基質細胞之方法
US8961956B2 (en) 2011-11-30 2015-02-24 Ocata Therapeutics, Inc. Mesenchymal stromal cells and uses related thereto
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GB201210857D0 (en) 2012-06-19 2012-08-01 Cambridge Entpr Ltd Transcription factor mediated programming towards megakaryocytes
CA2876499C (en) 2012-07-12 2021-10-26 Imstem Biotechnology, Inc. Mesenchymal-like stem cells derived from human embryonic stem cells, methods and uses thereof
KR20140011912A (ko) 2012-07-20 2014-01-29 도꾸리쯔교세이호징 리가가쿠 겐큐소 인간 적혈구 전구세포주 및 인간 탈핵 적혈구의 제조방법
GB201212960D0 (en) 2012-07-20 2012-09-05 Common Services Agency Erythroid production
US9664671B2 (en) 2012-07-24 2017-05-30 Nissan Chemical Industries, Ltd. Culture medium composition and method of culturing cell or tissue using thereof
US10017805B2 (en) 2012-08-23 2018-07-10 Nissan Chemical Industries, Ltd. Enhancing ingredients for protein production from various cells
CN114558032B (zh) 2012-12-21 2025-07-29 安斯泰来再生医药协会 由多能干细胞制备血小板的方法及其组合物
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MX366900B (es) * 2013-03-13 2019-07-30 Wisconsin Alumni Res Found Métodos y materiales para diferenciación hematoendotelial de células germinales pluripotentes humanas bajo condiciones definidas.
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