CA2710571C - Osteogenic differentiation of bone marrow stem cells and mesenchymal stem cells using a combination of growth factors - Google Patents

Osteogenic differentiation of bone marrow stem cells and mesenchymal stem cells using a combination of growth factors Download PDF

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Publication number
CA2710571C
CA2710571C CA2710571A CA2710571A CA2710571C CA 2710571 C CA2710571 C CA 2710571C CA 2710571 A CA2710571 A CA 2710571A CA 2710571 A CA2710571 A CA 2710571A CA 2710571 C CA2710571 C CA 2710571C
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cells
bone
tgfb
cell population
osteoblasts
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French (fr)
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CA2710571A1 (en
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Cindy Badoer
Enrico Bastianelli
Xavier Pesesse
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Bone Therapeutics SA
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Bone Therapeutics SA
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0654—Osteocytes, Osteoblasts, Odontocytes; Bones, Teeth
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0664—Dental pulp stem cells, Dental follicle stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0665—Blood-borne mesenchymal stem cells, e.g. from umbilical cord blood
    • C—CHEMISTRY; METALLURGY
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0666—Mesenchymal stem cells from hair follicles
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0668—Mesenchymal stem cells from other natural sources
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0688—Cells from the lungs or the respiratory tract
    • C12N5/0689—Stem cells; Progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/113—Acidic fibroblast growth factor (aFGF, FGF-1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/148—Transforming growth factor alpha [TGF-a]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C—CHEMISTRY; METALLURGY
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1346—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from mesenchymal stem cells

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  • Health & Medical Sciences (AREA)
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  • Medicinal Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
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  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Pulmonology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Surgical Instruments (AREA)
CA2710571A 2008-01-11 2009-01-09 Osteogenic differentiation of bone marrow stem cells and mesenchymal stem cells using a combination of growth factors Active CA2710571C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP08000458 2008-01-11
EP08000458.3 2008-01-11
PCT/EP2009/050209 WO2009087213A1 (en) 2008-01-11 2009-01-09 Osteogenic differentiation of bone marrow stem cells and mesenchymal stem cells using a combination of growth factors

Publications (2)

Publication Number Publication Date
CA2710571A1 CA2710571A1 (en) 2009-07-16
CA2710571C true CA2710571C (en) 2017-11-07

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CA2710571A Active CA2710571C (en) 2008-01-11 2009-01-09 Osteogenic differentiation of bone marrow stem cells and mesenchymal stem cells using a combination of growth factors

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US (3) US20100278788A1 (enExample)
EP (1) EP2229433B1 (enExample)
JP (1) JP5524079B2 (enExample)
KR (3) KR20180086305A (enExample)
CN (2) CN104250633A (enExample)
AU (2) AU2009203682B2 (enExample)
CA (1) CA2710571C (enExample)
DK (1) DK2229433T3 (enExample)
ES (1) ES2650235T3 (enExample)
NO (1) NO2229433T3 (enExample)
PL (1) PL2229433T3 (enExample)
PT (1) PT2229433T (enExample)
WO (1) WO2009087213A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010051032A1 (en) 2008-10-31 2010-05-06 Synthes Usa, Llc Method and device for activating stem cells
EP4086338A1 (en) 2011-03-17 2022-11-09 Minerva Biotechnologies Corporation Method for making pluripotent stem cells
JP2014530065A (ja) 2011-10-11 2014-11-17 ボーン セラピューティクスソシエテ アノニム 増殖分化因子８（ｇｄｆ‐８）の使用
EP2768945B1 (en) * 2011-10-17 2022-01-05 Minerva Biotechnologies Corporation Media for stem cell proliferation and induction
AU2016251313B2 (en) * 2015-04-23 2018-05-17 Bone Therapeutics S.A. In vitro preservation of therapeutic cells
CA3030866A1 (en) 2016-07-21 2018-01-25 Sanbio, Inc. Msc growth predictor assays
SG11202003524XA (en) 2017-10-20 2020-05-28 Bone Therapeutics Sa Methods for differentiating mesenchymal stem cells
JP2022502034A (ja) * 2018-09-25 2022-01-11 ボーン、セラピューティクス、エスアーBone Thrapeutics Sa ｉｎ ｖｉｔｒｏ分化細胞の骨形成能を決定するための方法および使用
BE1026595B9 (fr) 2018-09-25 2020-11-03 Bone Therapeutics Sa Procedes de differenciation de cellules souches mesenchymateuses
CN111357990A (zh) * 2019-12-26 2020-07-03 浙江欧谱生物科技有限公司 增加骨量的保健食品组合物及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5486359A (en) * 1990-11-16 1996-01-23 Osiris Therapeutics, Inc. Human mesenchymal stem cells
US5837539A (en) * 1990-11-16 1998-11-17 Osiris Therapeutics, Inc. Monoclonal antibodies for human mesenchymal stem cells
US5811094A (en) * 1990-11-16 1998-09-22 Osiris Therapeutics, Inc. Connective tissue regeneration using human mesenchymal stem cell preparations
US5972703A (en) * 1994-08-12 1999-10-26 The Regents Of The University Of Michigan Bone precursor cells: compositions and methods
US5736396A (en) * 1995-01-24 1998-04-07 Case Western Reserve University Lineage-directed induction of human mesenchymal stem cell differentiation
US5827740A (en) * 1996-07-30 1998-10-27 Osiris Therapeutics, Inc. Adipogenic differentiation of human mesenchymal stem cells
AU6144698A (en) * 1997-02-05 1998-08-25 Case Western Reserve University Stimulatory effects of bfgf and bmp-2 on osteogenic differentiation of mesenchymal stem cells
JP2007509609A (ja) * 2003-10-02 2007-04-19 ザ、リージェンツ、オブ、ザ、ユニバーシティ、オブ、ミシガン 幹細胞拘束および骨形成を誘導するヒト前立腺癌細胞因子
JP2006346420A (ja) * 2005-06-13 2006-12-28 Univ Nagoya 移植材料及び骨質改善剤
WO2007002210A2 (en) * 2005-06-20 2007-01-04 Bresagen, Inc. Embryonic stem cell culture compositions and methods of use thereof
TW200726474A (en) * 2005-07-15 2007-07-16 Cognate Therapeutics Inc The immunophenotype and immunogenicity of human adipose derived cells
PL1989295T3 (pl) * 2006-02-16 2021-02-22 Université Libre de Bruxelles Różnicowanie osteogenne komórek macierzystych szpiku kostnego oraz komórki i populacje osteoprogenitorowe lub osteoblastyczne

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Publication number Publication date
JP5524079B2 (ja) 2014-06-18
KR101920128B1 (ko) 2018-11-19
KR20100126680A (ko) 2010-12-02
DK2229433T3 (da) 2017-11-13
WO2009087213A1 (en) 2009-07-16
KR20180086305A (ko) 2018-07-30
AU2009203682B2 (en) 2014-07-03
CA2710571A1 (en) 2009-07-16
US20130266544A1 (en) 2013-10-10
CN104250633A (zh) 2014-12-31
ES2650235T3 (es) 2018-01-17
US20100278788A1 (en) 2010-11-04
AU2014204491A1 (en) 2014-08-07
EP2229433A1 (en) 2010-09-22
JP2011509656A (ja) 2011-03-31
US20200407688A1 (en) 2020-12-31
CN101945993A (zh) 2011-01-12
KR20160039699A (ko) 2016-04-11
PL2229433T3 (pl) 2018-01-31
NO2229433T3 (enExample) 2018-02-17
EP2229433B1 (en) 2017-09-20
PT2229433T (pt) 2017-12-12
AU2014204491B2 (en) 2016-03-24
AU2009203682A1 (en) 2009-07-16

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