CA2708354C - Means for delivery of nucleic acids active for gene silencing using synthetic polymers - Google Patents
Means for delivery of nucleic acids active for gene silencing using synthetic polymers Download PDFInfo
- Publication number
- CA2708354C CA2708354C CA2708354A CA2708354A CA2708354C CA 2708354 C CA2708354 C CA 2708354C CA 2708354 A CA2708354 A CA 2708354A CA 2708354 A CA2708354 A CA 2708354A CA 2708354 C CA2708354 C CA 2708354C
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- CA
- Canada
- Prior art keywords
- sirna
- tyrosine
- rna
- tryptophan
- polyethylenimine
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61K48/0008—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition
- A61K48/0025—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid
- A61K48/0041—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy characterised by an aspect of the 'non-active' part of the composition delivered, e.g. wherein such 'non-active' part is not delivered simultaneously with the 'active' part of the composition wherein the non-active part clearly interacts with the delivered nucleic acid the non-active part being polymeric
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/111—General methods applicable to biologically active non-coding nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2320/00—Applications; Uses
- C12N2320/30—Special therapeutic applications
- C12N2320/32—Special delivery means, e.g. tissue-specific
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Polyamides (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1335807P | 2007-12-13 | 2007-12-13 | |
| US61/013,358 | 2007-12-13 | ||
| PCT/IB2008/055256 WO2009074970A2 (en) | 2007-12-13 | 2008-12-12 | Means for delivery of nucleic acids active for gene silencing using synthetic polymers |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2708354A1 CA2708354A1 (en) | 2009-06-18 |
| CA2708354C true CA2708354C (en) | 2018-12-11 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2708354A Active CA2708354C (en) | 2007-12-13 | 2008-12-12 | Means for delivery of nucleic acids active for gene silencing using synthetic polymers |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20100297756A1 (enExample) |
| EP (1) | EP2229445B1 (enExample) |
| JP (1) | JP5802389B2 (enExample) |
| KR (1) | KR101590652B1 (enExample) |
| CN (1) | CN101952434B (enExample) |
| CA (1) | CA2708354C (enExample) |
| IL (1) | IL206310A (enExample) |
| WO (1) | WO2009074970A2 (enExample) |
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| EP2552460A1 (en) | 2010-03-29 | 2013-02-06 | Universite De Strasbourg | Polymers for delivering molecules of interest |
| KR101223483B1 (ko) * | 2010-09-10 | 2013-01-17 | 한국과학기술연구원 | 전하결합 및 생분해성 공유결합으로 동시에 연결된 고분자―ｓｉＲＮＡ 나노입자 전달체 |
| KR101497668B1 (ko) * | 2012-09-28 | 2015-03-03 | 한양대학교 산학협력단 | 전신 순환을 위한 짧은 간섭 rna 유전자 전달체 |
| WO2014051318A2 (ko) * | 2012-09-28 | 2014-04-03 | 한양대학교 산학협력단 | 전신 순환을 위한 짧은 간섭 rna 유전자 전달체 |
| EP4085930A1 (en) | 2012-10-05 | 2022-11-09 | BioNTech Delivery Technologies GmbH | Hydroxylated polyamine derivatives as transfection reagents for rna |
| CN104703629B (zh) | 2012-10-08 | 2020-12-01 | 生物技术传送科技有限责任公司 | 作为转染试剂的羧化多胺衍生物 |
| CN103087314B (zh) * | 2013-02-01 | 2015-02-18 | 同济大学 | 生物可降解聚氨酯基因转染试剂的制备方法 |
| CN107805643B (zh) * | 2017-09-15 | 2020-12-08 | 四川大学 | 靶向抑制沙门氏菌耐药外排泵基因acrA的siRNA-DNA纳米系统及其制备方法 |
| BR112022002149A2 (pt) * | 2019-08-05 | 2022-04-19 | Polyplus Transfection | Composição adequada para transfectar uma molécula de ácido nucleico em uma célula, métodos para transfecção in vitro ou ex vivo de células vivas e para a produção e usos in vitro ou ex vivo da composição |
| DE102020114183B4 (de) | 2020-05-27 | 2022-07-14 | Universität Leipzig Dezernat Forschung und Transfer | Mittel zur transfektion von nukleinsäuren in zellen |
| CN112195193A (zh) * | 2020-09-30 | 2021-01-08 | 上海交通大学 | 单链dna在基因转染中的应用 |
| US20240327830A1 (en) * | 2022-02-28 | 2024-10-03 | Northwestern University | Dise-inducing srna-polyplexes and srna-lipopolyplexes and methods of using the same to treat cancer |
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| US7427394B2 (en) * | 2000-10-10 | 2008-09-23 | Massachusetts Institute Of Technology | Biodegradable poly(β-amino esters) and uses thereof |
| US7601367B2 (en) * | 2002-05-28 | 2009-10-13 | Mirus Bio Llc | Compositions and processes using siRNA, amphipathic compounds and polycations |
| EP1546173A4 (en) * | 2002-08-06 | 2006-05-31 | Intradigm Corp | METHOD FOR DOWNSTREGULATING THE EXPRESSION OF TARGET GENES IN VIVO BY INCLUDING INTERFERING RNA |
| JP2006517793A (ja) * | 2003-01-31 | 2006-08-03 | ノバルティス アクチエンゲゼルシャフト | スクリーニングアッセイ |
| JP2007502129A (ja) * | 2003-05-09 | 2007-02-08 | ユニヴァーシティ オヴ ピッツバーグ オヴ ザ コモンウェルス システム オヴ ハイアー エデュケーション | 短鎖干渉ｒｎａライブラリーならびに合成および使用の方法 |
| SG190613A1 (en) * | 2003-07-16 | 2013-06-28 | Protiva Biotherapeutics Inc | Lipid encapsulated interfering rna |
| WO2005059111A2 (en) * | 2003-12-17 | 2005-06-30 | The Trustees Of Columbia University In The City Of New York | DELIVERY OF DNA OR RNA VIA GAP JUNCTIONS FROM HOST CELLS TO TARGET CELLS AND A CELL-BASED DELIVERY SYSTEM FOR ANTISENSE OR SiRNA |
| JP2008527993A (ja) * | 2005-01-24 | 2008-07-31 | アヴァリス・アクチエボラーグ | 特異性及び送達の改善のためのｓｉＲＮＡ分子、ｓｈＲＮＡ分子又はアンチセンス分子及び機能的実体を含む複合体 |
| US20100310670A1 (en) * | 2005-09-02 | 2010-12-09 | Takeda Pharmaceutical Company Limited | Sustained-release microsphere containing short chain deoxyribonucleic acid or short chain ribonucleic acid and method of producing the same |
| KR100863405B1 (ko) * | 2005-09-12 | 2008-10-14 | 주식회사 대웅 | 암 진단 마커 및 이의 이용 |
| JP2007119396A (ja) * | 2005-10-28 | 2007-05-17 | Hosokawa Funtai Gijutsu Kenkyusho:Kk | 核酸化合物封入ナノ粒子を含む経肺投与用医薬製剤 |
| GB0613753D0 (en) * | 2006-07-11 | 2006-08-23 | Norwegian Radium Hospital Res | Method |
| CN101085356A (zh) * | 2007-06-21 | 2007-12-12 | 中国科学院长春应用化学研究所 | 一种非病毒基因转染载体、其与质粒dna复合物颗粒及制备方法和用法 |
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2008
- 2008-12-12 EP EP08860093A patent/EP2229445B1/en active Active
- 2008-12-12 US US12/735,031 patent/US20100297756A1/en not_active Abandoned
- 2008-12-12 CN CN200880125788.1A patent/CN101952434B/zh active Active
- 2008-12-12 KR KR1020107015535A patent/KR101590652B1/ko active Active
- 2008-12-12 CA CA2708354A patent/CA2708354C/en active Active
- 2008-12-12 JP JP2010537590A patent/JP5802389B2/ja active Active
- 2008-12-12 WO PCT/IB2008/055256 patent/WO2009074970A2/en not_active Ceased
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2010
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Also Published As
| Publication number | Publication date |
|---|---|
| IL206310A (en) | 2013-07-31 |
| KR101590652B1 (ko) | 2016-02-18 |
| CN101952434A (zh) | 2011-01-19 |
| US20100297756A1 (en) | 2010-11-25 |
| KR20100105674A (ko) | 2010-09-29 |
| JP5802389B2 (ja) | 2015-10-28 |
| WO2009074970A3 (en) | 2009-10-29 |
| EP2229445B1 (en) | 2012-12-05 |
| JP2011505826A (ja) | 2011-03-03 |
| EP2229445A2 (en) | 2010-09-22 |
| WO2009074970A2 (en) | 2009-06-18 |
| IL206310A0 (en) | 2010-12-30 |
| CA2708354A1 (en) | 2009-06-18 |
| CN101952434B (zh) | 2016-11-16 |
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