CA2648332A1 - Approche basee sur la forme pour ingenierie tissulaire sans echafaudage - Google Patents
Approche basee sur la forme pour ingenierie tissulaire sans echafaudage Download PDFInfo
- Publication number
- CA2648332A1 CA2648332A1 CA002648332A CA2648332A CA2648332A1 CA 2648332 A1 CA2648332 A1 CA 2648332A1 CA 002648332 A CA002648332 A CA 002648332A CA 2648332 A CA2648332 A CA 2648332A CA 2648332 A1 CA2648332 A1 CA 2648332A1
- Authority
- CA
- Canada
- Prior art keywords
- cells
- constructs
- construct
- hydrogel
- tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000013459 approach Methods 0.000 title description 5
- 239000000017 hydrogel Substances 0.000 claims abstract description 67
- 238000000034 method Methods 0.000 claims abstract description 54
- 238000010899 nucleation Methods 0.000 claims abstract description 12
- 210000004027 cell Anatomy 0.000 claims description 89
- 210000001519 tissue Anatomy 0.000 claims description 80
- 229920000936 Agarose Polymers 0.000 claims description 60
- 210000001612 chondrocyte Anatomy 0.000 claims description 46
- 210000000689 upper leg Anatomy 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 5
- 210000003041 ligament Anatomy 0.000 claims description 4
- 210000002435 tendon Anatomy 0.000 claims description 4
- 210000004353 tibial menisci Anatomy 0.000 claims description 4
- HKSZLNNOFSGOKW-UHFFFAOYSA-N ent-staurosporine Natural products C12=C3N4C5=CC=CC=C5C3=C3CNC(=O)C3=C2C2=CC=CC=C2N1C1CC(NC)C(OC)C4(C)O1 HKSZLNNOFSGOKW-UHFFFAOYSA-N 0.000 claims description 3
- HKSZLNNOFSGOKW-FYTWVXJKSA-N staurosporine Chemical compound C12=C3N4C5=CC=CC=C5C3=C3CNC(=O)C3=C2C2=CC=CC=C2N1[C@H]1C[C@@H](NC)[C@@H](OC)[C@]4(C)O1 HKSZLNNOFSGOKW-FYTWVXJKSA-N 0.000 claims description 3
- CGPUWJWCVCFERF-UHFFFAOYSA-N staurosporine Natural products C12=C3N4C5=CC=CC=C5C3=C3CNC(=O)C3=C2C2=CC=CC=C2N1C1CC(NC)C(OC)C4(OC)O1 CGPUWJWCVCFERF-UHFFFAOYSA-N 0.000 claims description 3
- 210000001738 temporomandibular joint Anatomy 0.000 claims description 3
- 239000011248 coating agent Substances 0.000 claims description 2
- 238000000576 coating method Methods 0.000 claims description 2
- 241000124008 Mammalia Species 0.000 claims 6
- 238000012258 culturing Methods 0.000 claims 1
- 102000008186 Collagen Human genes 0.000 description 38
- 108010035532 Collagen Proteins 0.000 description 38
- 229920001436 collagen Polymers 0.000 description 38
- 238000010186 staining Methods 0.000 description 15
- 238000001338 self-assembly Methods 0.000 description 13
- 108010041390 Collagen Type II Proteins 0.000 description 12
- 102000000503 Collagen Type II Human genes 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 12
- 238000012360 testing method Methods 0.000 description 12
- 238000011282 treatment Methods 0.000 description 12
- 230000015572 biosynthetic process Effects 0.000 description 11
- 239000001963 growth medium Substances 0.000 description 10
- 239000002609 medium Substances 0.000 description 10
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 9
- 229920002683 Glycosaminoglycan Polymers 0.000 description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 210000000845 cartilage Anatomy 0.000 description 9
- 238000003364 immunohistochemistry Methods 0.000 description 9
- 230000008901 benefit Effects 0.000 description 8
- 239000000523 sample Substances 0.000 description 8
- 102000012422 Collagen Type I Human genes 0.000 description 7
- 108010022452 Collagen Type I Proteins 0.000 description 7
- 210000001188 articular cartilage Anatomy 0.000 description 7
- 230000007423 decrease Effects 0.000 description 7
- 230000003247 decreasing effect Effects 0.000 description 7
- 230000005499 meniscus Effects 0.000 description 7
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 6
- RZSYLLSAWYUBPE-UHFFFAOYSA-L Fast green FCF Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC(O)=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 RZSYLLSAWYUBPE-UHFFFAOYSA-L 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 229940096422 collagen type i Drugs 0.000 description 6
- 239000012091 fetal bovine serum Substances 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- OARRHUQTFTUEOS-UHFFFAOYSA-N safranin Chemical compound [Cl-].C=12C=C(N)C(C)=CC2=NC2=CC(C)=C(N)C=C2[N+]=1C1=CC=CC=C1 OARRHUQTFTUEOS-UHFFFAOYSA-N 0.000 description 6
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 244000309466 calf Species 0.000 description 5
- 238000003501 co-culture Methods 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 238000007373 indentation Methods 0.000 description 5
- 210000002414 leg Anatomy 0.000 description 5
- 230000035699 permeability Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 102000029816 Collagenase Human genes 0.000 description 4
- 108060005980 Collagenase Proteins 0.000 description 4
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 4
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 4
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 4
- ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 4
- 230000001070 adhesive effect Effects 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000002051 biphasic effect Effects 0.000 description 4
- 229960002424 collagenase Drugs 0.000 description 4
- 238000006073 displacement reaction Methods 0.000 description 4
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 4
- 239000003102 growth factor Substances 0.000 description 4
- 229960002591 hydroxyproline Drugs 0.000 description 4
- 210000003127 knee Anatomy 0.000 description 4
- 238000011068 loading method Methods 0.000 description 4
- 229910001220 stainless steel Inorganic materials 0.000 description 4
- 239000010935 stainless steel Substances 0.000 description 4
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 4
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 3
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 239000007995 HEPES buffer Substances 0.000 description 3
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 3
- 239000002211 L-ascorbic acid Substances 0.000 description 3
- 235000000069 L-ascorbic acid Nutrition 0.000 description 3
- WQZGKKKJIJFFOK-ZZWDRFIYSA-N L-glucose Chemical compound OC[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@H]1O WQZGKKKJIJFFOK-ZZWDRFIYSA-N 0.000 description 3
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 3
- 229930182816 L-glutamine Natural products 0.000 description 3
- 108090000526 Papain Proteins 0.000 description 3
- 229930182555 Penicillin Natural products 0.000 description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 3
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
- JRMSLDWZFJZLAS-UHFFFAOYSA-M [7-(dimethylamino)-1,9-dimethylphenothiazin-3-ylidene]-dimethylazanium;chloride Chemical compound [Cl-].CC1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC(C)=C3N=C21 JRMSLDWZFJZLAS-UHFFFAOYSA-M 0.000 description 3
- 229960004308 acetylcysteine Drugs 0.000 description 3
- APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 3
- 229960005070 ascorbic acid Drugs 0.000 description 3
- 238000003149 assay kit Methods 0.000 description 3
- 238000002737 cell proliferation kit Methods 0.000 description 3
- 230000006835 compression Effects 0.000 description 3
- 238000007906 compression Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 239000003797 essential amino acid Substances 0.000 description 3
- 235000020776 essential amino acid Nutrition 0.000 description 3
- 210000002744 extracellular matrix Anatomy 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 3
- 238000002513 implantation Methods 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 230000010354 integration Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000011159 matrix material Substances 0.000 description 3
- 239000003068 molecular probe Substances 0.000 description 3
- 229940055729 papain Drugs 0.000 description 3
- 235000019834 papain Nutrition 0.000 description 3
- 229940049954 penicillin Drugs 0.000 description 3
- 239000008363 phosphate buffer Substances 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 229910052710 silicon Inorganic materials 0.000 description 3
- 239000010703 silicon Substances 0.000 description 3
- 229960005322 streptomycin Drugs 0.000 description 3
- 230000035899 viability Effects 0.000 description 3
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 2
- 102000037716 Chondroitin-sulfate-ABC endolyases Human genes 0.000 description 2
- 108090000819 Chondroitin-sulfate-ABC endolyases Proteins 0.000 description 2
- 229920001651 Cyanoacrylate Polymers 0.000 description 2
- 241000283073 Equus caballus Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 101000883515 Homo sapiens Chitinase-3-like protein 1 Proteins 0.000 description 2
- MWCLLHOVUTZFKS-UHFFFAOYSA-N Methyl cyanoacrylate Chemical compound COC(=O)C(=C)C#N MWCLLHOVUTZFKS-UHFFFAOYSA-N 0.000 description 2
- 102000003992 Peroxidases Human genes 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 230000000181 anti-adherent effect Effects 0.000 description 2
- 230000003367 anti-collagen effect Effects 0.000 description 2
- AGSPXMVUFBBBMO-UHFFFAOYSA-N beta-aminopropionitrile Chemical compound NCCC#N AGSPXMVUFBBBMO-UHFFFAOYSA-N 0.000 description 2
- 238000001574 biopsy Methods 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- PBAYDYUZOSNJGU-UHFFFAOYSA-N chelidonic acid Natural products OC(=O)C1=CC(=O)C=C(C(O)=O)O1 PBAYDYUZOSNJGU-UHFFFAOYSA-N 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- VDQQXEISLMTGAB-UHFFFAOYSA-N chloramine T Chemical compound [Na+].CC1=CC=C(S(=O)(=O)[N-]Cl)C=C1 VDQQXEISLMTGAB-UHFFFAOYSA-N 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000000968 fibrocartilage Anatomy 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000014509 gene expression Effects 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 102000054350 human CHI3L1 Human genes 0.000 description 2
- 230000002706 hydrostatic effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 108040007629 peroxidase activity proteins Proteins 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000003307 slaughter Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 230000017423 tissue regeneration Effects 0.000 description 2
- 229960001479 tosylchloramide sodium Drugs 0.000 description 2
- 101710175516 14 kDa zinc-binding protein Proteins 0.000 description 1
- SKIIKRJAQOSWFT-UHFFFAOYSA-N 2-[3-[1-(2,2-difluoroethyl)piperidin-4-yl]oxy-4-[2-(2,3-dihydro-1H-inden-2-ylamino)pyrimidin-5-yl]pyrazol-1-yl]-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)ethanone Chemical compound FC(CN1CCC(CC1)OC1=NN(C=C1C=1C=NC(=NC=1)NC1CC2=CC=CC=C2C1)CC(=O)N1CC2=C(CC1)NN=N2)F SKIIKRJAQOSWFT-UHFFFAOYSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 102100029136 Collagen alpha-1(II) chain Human genes 0.000 description 1
- 102000002585 Contractile Proteins Human genes 0.000 description 1
- 108010068426 Contractile Proteins Proteins 0.000 description 1
- 239000004971 Cross linker Substances 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 101000771163 Homo sapiens Collagen alpha-1(II) chain Proteins 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102000004218 Insulin-Like Growth Factor I Human genes 0.000 description 1
- 241001484259 Lacuna Species 0.000 description 1
- 229940121922 Lysyl oxidase inhibitor Drugs 0.000 description 1
- 229940123924 Protein kinase C inhibitor Drugs 0.000 description 1
- YIQKLZYTHXTDDT-UHFFFAOYSA-H Sirius red F3B Chemical compound C1=CC(=CC=C1N=NC2=CC(=C(C=C2)N=NC3=C(C=C4C=C(C=CC4=C3[O-])NC(=O)NC5=CC6=CC(=C(C(=C6C=C5)[O-])N=NC7=C(C=C(C=C7)N=NC8=CC=C(C=C8)S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)O)S(=O)(=O)O)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+] YIQKLZYTHXTDDT-UHFFFAOYSA-H 0.000 description 1
- GLNADSQYFUSGOU-GPTZEZBUSA-J Trypan blue Chemical compound [Na+].[Na+].[Na+].[Na+].C1=C(S([O-])(=O)=O)C=C2C=C(S([O-])(=O)=O)C(/N=N/C3=CC=C(C=C3C)C=3C=C(C(=CC=3)\N=N\C=3C(=CC4=CC(=CC(N)=C4C=3O)S([O-])(=O)=O)S([O-])(=O)=O)C)=C(O)C2=C1N GLNADSQYFUSGOU-GPTZEZBUSA-J 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000035508 accumulation Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000012867 bioactive agent Substances 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000003848 cartilage regeneration Effects 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000021164 cell adhesion Effects 0.000 description 1
- 230000006727 cell loss Effects 0.000 description 1
- 230000008619 cell matrix interaction Effects 0.000 description 1
- 230000012292 cell migration Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000005754 cellular signaling Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000004624 confocal microscopy Methods 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 239000013068 control sample Substances 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 238000001804 debridement Methods 0.000 description 1
- 230000032459 dedifferentiation Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010874 in vitro model Methods 0.000 description 1
- 230000028709 inflammatory response Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000000629 knee joint Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005461 lubrication Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000005226 mechanical processes and functions Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000011206 morphological examination Methods 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 201000008482 osteoarthritis Diseases 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 239000003881 protein kinase C inhibitor Substances 0.000 description 1
- 230000036647 reaction Effects 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000008263 repair mechanism Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000004626 scanning electron microscopy Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000013424 sirius red staining Methods 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229920002994 synthetic fiber Polymers 0.000 description 1
- 229920001059 synthetic polymer Polymers 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 230000008733 trauma Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3817—Cartilage-forming cells, e.g. pre-chondrocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
- A61L27/3843—Connective tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/76—Agarose, agar-agar
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Animal Behavior & Ethology (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Rheumatology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Urology & Nephrology (AREA)
- Microbiology (AREA)
- Vascular Medicine (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
- Prostheses (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US78985306P | 2006-04-05 | 2006-04-05 | |
| US78985106P | 2006-04-05 | 2006-04-05 | |
| US78985506P | 2006-04-05 | 2006-04-05 | |
| US60/789,855 | 2006-04-05 | ||
| US60/789,851 | 2006-04-05 | ||
| US60/789,853 | 2006-04-05 | ||
| PCT/US2007/066089 WO2007115336A2 (fr) | 2004-07-09 | 2007-04-05 | Approche basée sur la forme pour ingéniérie tissulaire sans échafaudage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2648332A1 true CA2648332A1 (fr) | 2007-10-11 |
Family
ID=38564326
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002648648A Abandoned CA2648648A1 (fr) | 2006-04-05 | 2007-04-05 | Cellules derivees du derme pour des applications de genie histologique |
| CA002648332A Abandoned CA2648332A1 (fr) | 2006-04-05 | 2007-04-05 | Approche basee sur la forme pour ingenierie tissulaire sans echafaudage |
| CA002648327A Abandoned CA2648327A1 (fr) | 2006-04-05 | 2007-04-05 | Genie tissulaire a l'aide de cellules souches embryonnaires humaines |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002648648A Abandoned CA2648648A1 (fr) | 2006-04-05 | 2007-04-05 | Cellules derivees du derme pour des applications de genie histologique |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002648327A Abandoned CA2648327A1 (fr) | 2006-04-05 | 2007-04-05 | Genie tissulaire a l'aide de cellules souches embryonnaires humaines |
Country Status (4)
| Country | Link |
|---|---|
| EP (3) | EP2007312A4 (fr) |
| AU (3) | AU2007234365A1 (fr) |
| CA (3) | CA2648648A1 (fr) |
| WO (3) | WO2007115336A2 (fr) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2338441B1 (fr) | 2003-12-11 | 2013-01-23 | Isto Technologies Inc. | Système chondral particulier |
| WO2005081970A2 (fr) | 2004-02-24 | 2005-09-09 | The Curators Of The University Of Missouri | Agregats cellulaires d'auto-assemblage et procedes de fabrication de tissu genetiquement modifies utilisant de tels agregats |
| US20110212894A1 (en) * | 2004-07-09 | 2011-09-01 | Athanasiou Kyriacos A | Decellularization method for scaffoldless tissue engineered articular cartilage or native cartilage tissue |
| WO2010022074A1 (fr) * | 2008-08-18 | 2010-02-25 | William Marsh Rice University | Procédé de décellularisation pour cartilage articulaire modifié de tissu sans échafaudage ou pour tissu de cartilage natif |
| WO2007025290A2 (fr) | 2005-08-26 | 2007-03-01 | Isto Technologies, Inc. | Implants et procedes pour la reparation, le remplacement et le traitement de maladies articulaires |
| US8163549B2 (en) | 2006-12-20 | 2012-04-24 | Zimmer Orthobiologics, Inc. | Method of obtaining viable small tissue particles and use for tissue repair |
| AU2008240191B2 (en) | 2007-04-12 | 2013-09-19 | Zimmer, Inc. | Compositions and methods for tissue repair |
| EP2254508A4 (fr) * | 2008-03-03 | 2012-03-28 | Univ Rice William M | Procédés de fabrication d'un cartilage amélioré d'ingénierie tissulaire |
| SI2310491T1 (en) * | 2008-06-20 | 2018-07-31 | Universiteit Maastricht | Only composite tissue modules |
| AU2009271223B2 (en) | 2008-06-24 | 2013-05-16 | The Curators Of The University Of Missouri | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same |
| JP6096509B2 (ja) * | 2009-07-16 | 2017-03-15 | バイオタイム インク．Ｂｉｏｔｉｍｅ Ｉｎｃ． | インビトロ及び生体内での軟骨形成のための方法及び組成物 |
| CN105496601A (zh) | 2010-10-21 | 2016-04-20 | 奥加诺沃公司 | 用于制造组织的装置、系统和方法 |
| US10865383B2 (en) | 2011-07-12 | 2020-12-15 | Lineage Cell Therapeutics, Inc. | Methods and formulations for orthopedic cell therapy |
| US9499779B2 (en) | 2012-04-20 | 2016-11-22 | Organovo, Inc. | Devices, systems, and methods for the fabrication of tissue utilizing UV cross-linking |
| US20140178343A1 (en) | 2012-12-21 | 2014-06-26 | Jian Q. Yao | Supports and methods for promoting integration of cartilage tissue explants |
| US9442105B2 (en) | 2013-03-15 | 2016-09-13 | Organovo, Inc. | Engineered liver tissues, arrays thereof, and methods of making the same |
| CA2919734C (fr) | 2013-07-31 | 2023-04-25 | Organovo, Inc. | Dispositifs, systemes et procedes automatises pour la fabrication de tissus |
| EP3808838B1 (fr) | 2014-04-04 | 2024-04-10 | Organovo, Inc. | Tissu mammaire tridimensionnel artificiel, tissu adipeux, et modèle de maladies tumorales |
| US9670443B2 (en) | 2014-06-25 | 2017-06-06 | University Of Leeds | Tissue engineered constructs |
| EP3204488B1 (fr) | 2014-10-06 | 2019-07-17 | Organovo, Inc. | Tissus rénaux produits par génie génétique, réseaux correspondants, et procédés de production |
| US20160122723A1 (en) | 2014-11-05 | 2016-05-05 | Organovo, Inc. | Engineered three-dimensional skin tissues, arrays thereof, and methods of making the same |
| WO2017083402A1 (fr) | 2015-11-09 | 2017-05-18 | Organovo, Inc. | Procédés améliorés de fabrication de tissus |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69631490T2 (de) * | 1995-11-09 | 2004-10-07 | Univ Massachusetts Boston | Wiederherstellung der gewebeoberfläche mit zusammensetzungen aus hydrogel-zellen |
| WO2000052145A2 (fr) * | 1999-03-02 | 2000-09-08 | University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus | Lignees cellulaires embryonnaires ou de type souche produites par transplantation nucleaire d'especes croisees |
| US7045353B2 (en) * | 2000-08-01 | 2006-05-16 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Directed differentiation of human embryonic cells |
| AU2002258477A1 (en) * | 2001-03-08 | 2002-09-24 | Advanced Cell Technology, Inc. | Use of rna interference for the creation of lineage specific es and other undifferentiated cells and production of differentiated cells in vitro by co-culture |
| EP1463800A4 (fr) * | 2001-12-07 | 2006-04-26 | Geron Corp | Precurseurs de chondrocytes derives de cellules souches embryonnaires humaines |
| US20020106625A1 (en) * | 2002-02-07 | 2002-08-08 | Hung Clark T. | Bioreactor for generating functional cartilaginous tissue |
| WO2003084385A2 (fr) * | 2002-04-02 | 2003-10-16 | William Marsh Rice University | Cellules redifferenciees utilisees pour reparer les defauts du cartilage |
| US20040082063A1 (en) * | 2002-10-18 | 2004-04-29 | Reliance Life Sciences Pvt. Ltd. | Tissue-like organization of cells and macroscopic tissue-like constructs, generated by macromass culture of cells, and the method of macromass culture |
| WO2006017176A2 (fr) * | 2004-07-09 | 2006-02-16 | William Marsh Rice University | Constructions sans echafaudage pour genie tissulaire de cartilage articulaire |
-
2007
- 2007-04-05 CA CA002648648A patent/CA2648648A1/fr not_active Abandoned
- 2007-04-05 WO PCT/US2007/066089 patent/WO2007115336A2/fr active Application Filing
- 2007-04-05 EP EP20070760209 patent/EP2007312A4/fr not_active Withdrawn
- 2007-04-05 EP EP07760205A patent/EP2007875A4/fr not_active Withdrawn
- 2007-04-05 CA CA002648332A patent/CA2648332A1/fr not_active Abandoned
- 2007-04-05 AU AU2007234365A patent/AU2007234365A1/en not_active Abandoned
- 2007-04-05 AU AU2007254048A patent/AU2007254048A1/en not_active Abandoned
- 2007-04-05 WO PCT/US2007/066092 patent/WO2007115337A2/fr active Application Filing
- 2007-04-05 AU AU2007234366A patent/AU2007234366A1/en not_active Abandoned
- 2007-04-05 WO PCT/US2007/066085 patent/WO2007136936A2/fr active Application Filing
- 2007-04-05 CA CA002648327A patent/CA2648327A1/fr not_active Abandoned
- 2007-04-05 EP EP07760211A patent/EP2007881A4/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| AU2007234366A1 (en) | 2007-10-11 |
| EP2007875A2 (fr) | 2008-12-31 |
| WO2007115336A3 (fr) | 2008-05-22 |
| WO2007115337A2 (fr) | 2007-10-11 |
| CA2648327A1 (fr) | 2007-10-11 |
| EP2007881A4 (fr) | 2009-07-08 |
| AU2007234365A1 (en) | 2007-10-11 |
| WO2007115337A8 (fr) | 2007-12-13 |
| WO2007115337A3 (fr) | 2008-07-03 |
| EP2007312A2 (fr) | 2008-12-31 |
| EP2007312A4 (fr) | 2012-08-22 |
| WO2007115336A2 (fr) | 2007-10-11 |
| WO2007136936A2 (fr) | 2007-11-29 |
| WO2007136936A3 (fr) | 2008-10-23 |
| AU2007254048A1 (en) | 2007-11-29 |
| CA2648648A1 (fr) | 2007-11-29 |
| EP2007881A2 (fr) | 2008-12-31 |
| EP2007875A4 (fr) | 2009-12-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US20090142307A1 (en) | Shape-Based Approach for Scaffoldless Tissue Engineering | |
| CA2648332A1 (fr) | Approche basee sur la forme pour ingenierie tissulaire sans echafaudage | |
| Dunkelman et al. | Cartilage production by rabbit articular chondrocytes on polyglycolic acid scaffolds in a closed bioreactor system | |
| Flanagan et al. | A collagen-glycosaminoglycan co-culture model for heart valve tissue engineering applications | |
| Ibsirlioglu et al. | Decellularized biological scaffold and stem cells from autologous human adipose tissue for cartilage tissue engineering | |
| AU2002335747B2 (en) | Stratified cartilage tissue and methods to engineer same | |
| AU2002335747A1 (en) | Stratified cartilage tissue and methods to engineer same | |
| US12048779B2 (en) | Engineering mechanically functional human cartilage and method of making same | |
| EP2780048B1 (fr) | Petit tissu à base de dextrane contenant un lysat à plasma riche en plaquettes pour la réparation des cartilages | |
| Kinner et al. | Expression of smooth muscle actin in osteoblasts in human bone | |
| JP7058607B2 (ja) | カスタマイズされた骨－インプラントハイブリッド移植片 | |
| Nachtsheim et al. | Chondrocyte colonisation of a tissue-engineered cartilage substitute under a mechanical stimulus | |
| US11786636B2 (en) | Methods for complex tissue engineering | |
| US20090136559A1 (en) | Chondrocyte Differentiation from Human Embryonic Stem Cells and Their Use in Tissue Engineering | |
| WO2016161311A1 (fr) | Procédés in vitro pour évaluer la compatibilité tissulaire d'un matériau | |
| US8637065B2 (en) | Dermis-derived cells for tissue engineering applications | |
| JP2022550911A (ja) | 軟骨形成ヒト間葉系幹細胞（ｍｓｃ）シート | |
| Pleumeekers | Face it: Cell-based therapy for the reconstruction of cartilage defects in the head and neck area. | |
| Hoben | Self assembly of fibrochondrocytes and human embryonic stem cells for tissue engineering of fibrocartilage | |
| Russell | Re-engineering brain tissue | |
| Lima | The tissue-engineering of functional osteochondral constructs for cartilage repair |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |