CA2648327A1 - Genie tissulaire a l'aide de cellules souches embryonnaires humaines - Google Patents
Genie tissulaire a l'aide de cellules souches embryonnaires humaines Download PDFInfo
- Publication number
- CA2648327A1 CA2648327A1 CA002648327A CA2648327A CA2648327A1 CA 2648327 A1 CA2648327 A1 CA 2648327A1 CA 002648327 A CA002648327 A CA 002648327A CA 2648327 A CA2648327 A CA 2648327A CA 2648327 A1 CA2648327 A1 CA 2648327A1
- Authority
- CA
- Canada
- Prior art keywords
- constructs
- cells
- embryoid bodies
- embryonic stem
- construct
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 241000282414 Homo sapiens Species 0.000 title claims abstract description 49
- 210000001671 embryonic stem cell Anatomy 0.000 title claims abstract description 46
- 210000002242 embryoid body Anatomy 0.000 claims abstract description 147
- 230000004069 differentiation Effects 0.000 claims abstract description 66
- 238000000034 method Methods 0.000 claims abstract description 66
- 239000001963 growth medium Substances 0.000 claims abstract description 29
- 239000003102 growth factor Substances 0.000 claims abstract description 23
- 210000001612 chondrocyte Anatomy 0.000 claims abstract description 18
- 230000009816 chondrogenic differentiation Effects 0.000 claims abstract description 16
- 238000012258 culturing Methods 0.000 claims abstract description 8
- 230000004931 aggregating effect Effects 0.000 claims abstract description 6
- 230000001939 inductive effect Effects 0.000 claims abstract description 4
- 210000004027 cell Anatomy 0.000 claims description 123
- 239000000017 hydrogel Substances 0.000 claims description 34
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 27
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 claims description 27
- 239000012091 fetal bovine serum Substances 0.000 claims description 25
- 102000004218 Insulin-Like Growth Factor I Human genes 0.000 claims description 23
- 108010049931 Bone Morphogenetic Protein 2 Proteins 0.000 claims description 8
- 102100024506 Bone morphogenetic protein 2 Human genes 0.000 claims description 8
- 241000124008 Mammalia Species 0.000 claims description 4
- 238000010374 somatic cell nuclear transfer Methods 0.000 claims description 4
- HKSZLNNOFSGOKW-UHFFFAOYSA-N ent-staurosporine Natural products C12=C3N4C5=CC=CC=C5C3=C3CNC(=O)C3=C2C2=CC=CC=C2N1C1CC(NC)C(OC)C4(C)O1 HKSZLNNOFSGOKW-UHFFFAOYSA-N 0.000 claims description 3
- 238000000465 moulding Methods 0.000 claims description 3
- HKSZLNNOFSGOKW-FYTWVXJKSA-N staurosporine Chemical compound C12=C3N4C5=CC=CC=C5C3=C3CNC(=O)C3=C2C2=CC=CC=C2N1[C@H]1C[C@@H](NC)[C@@H](OC)[C@]4(C)O1 HKSZLNNOFSGOKW-FYTWVXJKSA-N 0.000 claims description 3
- CGPUWJWCVCFERF-UHFFFAOYSA-N staurosporine Natural products C12=C3N4C5=CC=CC=C5C3=C3CNC(=O)C3=C2C2=CC=CC=C2N1C1CC(NC)C(OC)C4(OC)O1 CGPUWJWCVCFERF-UHFFFAOYSA-N 0.000 claims description 3
- 210000003041 ligament Anatomy 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 210000001738 temporomandibular joint Anatomy 0.000 claims description 2
- 210000002435 tendon Anatomy 0.000 claims description 2
- -1 TGF-.beta.3 Proteins 0.000 claims 2
- 210000000689 upper leg Anatomy 0.000 claims 1
- 210000001519 tissue Anatomy 0.000 description 65
- 238000001338 self-assembly Methods 0.000 description 36
- 102000000503 Collagen Type II Human genes 0.000 description 35
- 108010041390 Collagen Type II Proteins 0.000 description 35
- 102000008186 Collagen Human genes 0.000 description 34
- 108010035532 Collagen Proteins 0.000 description 34
- 229920001436 collagen Polymers 0.000 description 34
- 210000000845 cartilage Anatomy 0.000 description 22
- 239000002609 medium Substances 0.000 description 21
- 238000010186 staining Methods 0.000 description 19
- 229920000936 Agarose Polymers 0.000 description 17
- 102000012422 Collagen Type I Human genes 0.000 description 17
- 108010022452 Collagen Type I Proteins 0.000 description 17
- 229940096422 collagen type i Drugs 0.000 description 17
- 210000000130 stem cell Anatomy 0.000 description 17
- 229920002683 Glycosaminoglycan Polymers 0.000 description 16
- 238000012360 testing method Methods 0.000 description 15
- 239000003795 chemical substances by application Substances 0.000 description 13
- 238000003364 immunohistochemistry Methods 0.000 description 13
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 12
- 238000004458 analytical method Methods 0.000 description 12
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 12
- 210000003205 muscle Anatomy 0.000 description 11
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 10
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 10
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 10
- 230000002648 chondrogenic effect Effects 0.000 description 10
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 10
- 238000010899 nucleation Methods 0.000 description 10
- 230000008569 process Effects 0.000 description 10
- 210000002966 serum Anatomy 0.000 description 10
- 210000001188 articular cartilage Anatomy 0.000 description 9
- 230000008901 benefit Effects 0.000 description 9
- 210000000988 bone and bone Anatomy 0.000 description 9
- 238000002965 ELISA Methods 0.000 description 8
- 230000006835 compression Effects 0.000 description 8
- 238000007906 compression Methods 0.000 description 8
- 210000002744 extracellular matrix Anatomy 0.000 description 8
- 230000014509 gene expression Effects 0.000 description 8
- YIQKLZYTHXTDDT-UHFFFAOYSA-H Sirius red F3B Chemical compound C1=CC(=CC=C1N=NC2=CC(=C(C=C2)N=NC3=C(C=C4C=C(C=CC4=C3[O-])NC(=O)NC5=CC6=CC(=C(C(=C6C=C5)[O-])N=NC7=C(C=C(C=C7)N=NC8=CC=C(C=C8)S(=O)(=O)[O-])S(=O)(=O)[O-])S(=O)(=O)O)S(=O)(=O)O)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+].[Na+].[Na+].[Na+].[Na+].[Na+] YIQKLZYTHXTDDT-UHFFFAOYSA-H 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 239000011159 matrix material Substances 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 6
- 101100096242 Mus musculus Sox9 gene Proteins 0.000 description 6
- UREBDLICKHMUKA-CXSFZGCWSA-N dexamethasone Chemical compound C1CC2=CC(=O)C=C[C@]2(C)[C@]2(F)[C@@H]1[C@@H]1C[C@@H](C)[C@@](C(=O)CO)(O)[C@@]1(C)C[C@@H]2O UREBDLICKHMUKA-CXSFZGCWSA-N 0.000 description 6
- 229960003957 dexamethasone Drugs 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 230000000877 morphologic effect Effects 0.000 description 6
- 229960002429 proline Drugs 0.000 description 6
- 229940054269 sodium pyruvate Drugs 0.000 description 6
- 230000035882 stress Effects 0.000 description 6
- 238000009864 tensile test Methods 0.000 description 6
- 238000011282 treatment Methods 0.000 description 6
- 102000004877 Insulin Human genes 0.000 description 5
- 108090001061 Insulin Proteins 0.000 description 5
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 5
- 229930182816 L-glutamine Natural products 0.000 description 5
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 description 5
- ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 description 5
- 102000004338 Transferrin Human genes 0.000 description 5
- 108090000901 Transferrin Proteins 0.000 description 5
- 229960005070 ascorbic acid Drugs 0.000 description 5
- 235000010323 ascorbic acid Nutrition 0.000 description 5
- 239000011668 ascorbic acid Substances 0.000 description 5
- 238000007373 indentation Methods 0.000 description 5
- 229940125396 insulin Drugs 0.000 description 5
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 description 5
- 235000020778 linoleic acid Nutrition 0.000 description 5
- 210000002894 multi-fate stem cell Anatomy 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 229940000207 selenious acid Drugs 0.000 description 5
- MCAHWIHFGHIESP-UHFFFAOYSA-N selenous acid Chemical compound O[Se](O)=O MCAHWIHFGHIESP-UHFFFAOYSA-N 0.000 description 5
- 239000012581 transferrin Substances 0.000 description 5
- 101100454433 Biomphalaria glabrata BG01 gene Proteins 0.000 description 4
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 4
- WQZGKKKJIJFFOK-ZZWDRFIYSA-N L-glucose Chemical compound OC[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@H]1O WQZGKKKJIJFFOK-ZZWDRFIYSA-N 0.000 description 4
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 4
- 102000013275 Somatomedins Human genes 0.000 description 4
- 102000009618 Transforming Growth Factors Human genes 0.000 description 4
- 108010009583 Transforming Growth Factors Proteins 0.000 description 4
- 239000000853 adhesive Substances 0.000 description 4
- 230000001070 adhesive effect Effects 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000000721 bacterilogical effect Effects 0.000 description 4
- 238000010256 biochemical assay Methods 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 108010007093 dispase Proteins 0.000 description 4
- 238000010494 dissociation reaction Methods 0.000 description 4
- 230000005593 dissociations Effects 0.000 description 4
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 4
- 210000002257 embryonic structure Anatomy 0.000 description 4
- 210000003754 fetus Anatomy 0.000 description 4
- 210000000968 fibrocartilage Anatomy 0.000 description 4
- 239000012530 fluid Substances 0.000 description 4
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 4
- 229960002591 hydroxyproline Drugs 0.000 description 4
- 238000002513 implantation Methods 0.000 description 4
- 230000005499 meniscus Effects 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 4
- 102000029816 Collagenase Human genes 0.000 description 3
- 108060005980 Collagenase Proteins 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 3
- 230000002051 biphasic effect Effects 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 229960002424 collagenase Drugs 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
- 230000003328 fibroblastic effect Effects 0.000 description 3
- 102000054350 human CHI3L1 Human genes 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- ZAINTDRBUHCDPZ-UHFFFAOYSA-M Alexa Fluor 546 Chemical compound [H+].[Na+].CC1CC(C)(C)NC(C(=C2OC3=C(C4=NC(C)(C)CC(C)C4=CC3=3)S([O-])(=O)=O)S([O-])(=O)=O)=C1C=C2C=3C(C(=C(Cl)C=1Cl)C(O)=O)=C(Cl)C=1SCC(=O)NCCCCCC(=O)ON1C(=O)CCC1=O ZAINTDRBUHCDPZ-UHFFFAOYSA-M 0.000 description 2
- 102000037716 Chondroitin-sulfate-ABC endolyases Human genes 0.000 description 2
- 108090000819 Chondroitin-sulfate-ABC endolyases Proteins 0.000 description 2
- 101000883515 Homo sapiens Chitinase-3-like protein 1 Proteins 0.000 description 2
- 229930182821 L-proline Natural products 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 238000010162 Tukey test Methods 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 210000000577 adipose tissue Anatomy 0.000 description 2
- KDXHLJMVLXJXCW-UHFFFAOYSA-J alcian blue stain Chemical compound [Cl-].[Cl-].[Cl-].[Cl-].[Cu+2].[N-]1C(N=C2C3=CC(CSC(N(C)C)=[N+](C)C)=CC=C3C(N=C3C4=CC=C(CSC(N(C)C)=[N+](C)C)C=C4C(=N4)[N-]3)=N2)=C(C=C(CSC(N(C)C)=[N+](C)C)C=C2)C2=C1N=C1C2=CC(CSC(N(C)C)=[N+](C)C)=CC=C2C4=N1 KDXHLJMVLXJXCW-UHFFFAOYSA-J 0.000 description 2
- 238000000540 analysis of variance Methods 0.000 description 2
- 230000000181 anti-adherent effect Effects 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- AGSPXMVUFBBBMO-UHFFFAOYSA-N beta-aminopropionitrile Chemical compound NCCC#N AGSPXMVUFBBBMO-UHFFFAOYSA-N 0.000 description 2
- 238000012742 biochemical analysis Methods 0.000 description 2
- 210000000601 blood cell Anatomy 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 229940098773 bovine serum albumin Drugs 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 230000002596 correlated effect Effects 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 210000004602 germ cell Anatomy 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 210000003127 knee Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000002826 placenta Anatomy 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001172 regenerating effect Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 230000007704 transition Effects 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 101710175516 14 kDa zinc-binding protein Proteins 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 108010067219 Aggrecans Proteins 0.000 description 1
- 102000016284 Aggrecans Human genes 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 206010007710 Cartilage injury Diseases 0.000 description 1
- 206010061762 Chondropathy Diseases 0.000 description 1
- 102000002585 Contractile Proteins Human genes 0.000 description 1
- 108010068426 Contractile Proteins Proteins 0.000 description 1
- 239000004971 Cross linker Substances 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- RZSYLLSAWYUBPE-UHFFFAOYSA-L Fast green FCF Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC(O)=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 RZSYLLSAWYUBPE-UHFFFAOYSA-L 0.000 description 1
- 102000020897 Formins Human genes 0.000 description 1
- 108091022623 Formins Proteins 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 238000010867 Hoechst staining Methods 0.000 description 1
- MIJPAVRNWPDMOR-ZAFYKAAXSA-N L-ascorbic acid 2-phosphate Chemical compound OC[C@H](O)[C@H]1OC(=O)C(OP(O)(O)=O)=C1O MIJPAVRNWPDMOR-ZAFYKAAXSA-N 0.000 description 1
- 229940121922 Lysyl oxidase inhibitor Drugs 0.000 description 1
- NPPQSCRMBWNHMW-UHFFFAOYSA-N Meprobamate Chemical compound NC(=O)OCC(C)(CCC)COC(N)=O NPPQSCRMBWNHMW-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- NPGIHFRTRXVWOY-UHFFFAOYSA-N Oil red O Chemical compound Cc1ccc(C)c(c1)N=Nc1cc(C)c(cc1C)N=Nc1c(O)ccc2ccccc12 NPGIHFRTRXVWOY-UHFFFAOYSA-N 0.000 description 1
- 102000016387 Pancreatic elastase Human genes 0.000 description 1
- 108010067372 Pancreatic elastase Proteins 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 229940123924 Protein kinase C inhibitor Drugs 0.000 description 1
- 102000016611 Proteoglycans Human genes 0.000 description 1
- 108010067787 Proteoglycans Proteins 0.000 description 1
- 102000012083 SOX9 Transcription Factor Human genes 0.000 description 1
- 108010036426 SOX9 Transcription Factor Proteins 0.000 description 1
- 230000035508 accumulation Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 238000010420 art technique Methods 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 239000012888 bovine serum Substances 0.000 description 1
- 210000004413 cardiac myocyte Anatomy 0.000 description 1
- 230000022159 cartilage development Effects 0.000 description 1
- 230000003848 cartilage regeneration Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000008619 cell matrix interaction Effects 0.000 description 1
- 230000012292 cell migration Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000004624 confocal microscopy Methods 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 238000001804 debridement Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- IPZJQDSFZGZEOY-UHFFFAOYSA-N dimethylmethylene Chemical group C[C]C IPZJQDSFZGZEOY-UHFFFAOYSA-N 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000003981 ectoderm Anatomy 0.000 description 1
- 210000002308 embryonic cell Anatomy 0.000 description 1
- 210000001900 endoderm Anatomy 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 210000001654 germ layer Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 210000004276 hyalin Anatomy 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 210000003716 mesoderm Anatomy 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 238000011206 morphological examination Methods 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 210000003757 neuroblast Anatomy 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229920002113 octoxynol Polymers 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000004789 organ system Anatomy 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 238000001907 polarising light microscopy Methods 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000003881 protein kinase C inhibitor Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- OARRHUQTFTUEOS-UHFFFAOYSA-N safranin Chemical compound [Cl-].C=12C=C(N)C(C)=CC2=NC2=CC(C)=C(N)C=C2[N+]=1C1=CC=CC=C1 OARRHUQTFTUEOS-UHFFFAOYSA-N 0.000 description 1
- 238000004626 scanning electron microscopy Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000013424 sirius red staining Methods 0.000 description 1
- 210000004927 skin cell Anatomy 0.000 description 1
- 238000012358 sourcing Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 210000005065 subchondral bone plate Anatomy 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3895—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells using specific culture conditions, e.g. stimulating differentiation of stem cells, pulsatile flow conditions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
- A61L27/3817—Cartilage-forming cells, e.g. pre-chondrocytes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
- A61L27/3843—Connective tissue
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
- A61L27/3886—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells comprising two or more cell types
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0655—Chondrocytes; Cartilage
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/70—Polysaccharides
- C12N2533/76—Agarose, agar-agar
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Dermatology (AREA)
- Medicinal Chemistry (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Rheumatology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Vascular Medicine (AREA)
- General Engineering & Computer Science (AREA)
- Developmental Biology & Embryology (AREA)
- Urology & Nephrology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Prostheses (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US78985306P | 2006-04-05 | 2006-04-05 | |
| US78985106P | 2006-04-05 | 2006-04-05 | |
| US78985506P | 2006-04-05 | 2006-04-05 | |
| US60/789,855 | 2006-04-05 | ||
| US60/789,851 | 2006-04-05 | ||
| US60/789,853 | 2006-04-05 | ||
| PCT/US2007/066092 WO2007115337A2 (fr) | 2006-04-05 | 2007-04-05 | Ingéniérie tissulaire utilisant des cellules souches embryonnaires humaines |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2648327A1 true CA2648327A1 (fr) | 2007-10-11 |
Family
ID=38564326
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002648648A Abandoned CA2648648A1 (fr) | 2006-04-05 | 2007-04-05 | Cellules derivees du derme pour des applications de genie histologique |
| CA002648332A Abandoned CA2648332A1 (fr) | 2006-04-05 | 2007-04-05 | Approche basee sur la forme pour ingenierie tissulaire sans echafaudage |
| CA002648327A Abandoned CA2648327A1 (fr) | 2006-04-05 | 2007-04-05 | Genie tissulaire a l'aide de cellules souches embryonnaires humaines |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002648648A Abandoned CA2648648A1 (fr) | 2006-04-05 | 2007-04-05 | Cellules derivees du derme pour des applications de genie histologique |
| CA002648332A Abandoned CA2648332A1 (fr) | 2006-04-05 | 2007-04-05 | Approche basee sur la forme pour ingenierie tissulaire sans echafaudage |
Country Status (4)
| Country | Link |
|---|---|
| EP (3) | EP2007312A4 (fr) |
| AU (3) | AU2007234365A1 (fr) |
| CA (3) | CA2648648A1 (fr) |
| WO (3) | WO2007115336A2 (fr) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2338441B1 (fr) | 2003-12-11 | 2013-01-23 | Isto Technologies Inc. | Système chondral particulier |
| WO2005081970A2 (fr) | 2004-02-24 | 2005-09-09 | The Curators Of The University Of Missouri | Agregats cellulaires d'auto-assemblage et procedes de fabrication de tissu genetiquement modifies utilisant de tels agregats |
| US20110212894A1 (en) * | 2004-07-09 | 2011-09-01 | Athanasiou Kyriacos A | Decellularization method for scaffoldless tissue engineered articular cartilage or native cartilage tissue |
| WO2010022074A1 (fr) * | 2008-08-18 | 2010-02-25 | William Marsh Rice University | Procédé de décellularisation pour cartilage articulaire modifié de tissu sans échafaudage ou pour tissu de cartilage natif |
| WO2007025290A2 (fr) | 2005-08-26 | 2007-03-01 | Isto Technologies, Inc. | Implants et procedes pour la reparation, le remplacement et le traitement de maladies articulaires |
| US8163549B2 (en) | 2006-12-20 | 2012-04-24 | Zimmer Orthobiologics, Inc. | Method of obtaining viable small tissue particles and use for tissue repair |
| AU2008240191B2 (en) | 2007-04-12 | 2013-09-19 | Zimmer, Inc. | Compositions and methods for tissue repair |
| EP2254508A4 (fr) * | 2008-03-03 | 2012-03-28 | Univ Rice William M | Procédés de fabrication d'un cartilage amélioré d'ingénierie tissulaire |
| SI2310491T1 (en) * | 2008-06-20 | 2018-07-31 | Universiteit Maastricht | Only composite tissue modules |
| AU2009271223B2 (en) | 2008-06-24 | 2013-05-16 | The Curators Of The University Of Missouri | Self-assembling multicellular bodies and methods of producing a three-dimensional biological structure using the same |
| JP6096509B2 (ja) * | 2009-07-16 | 2017-03-15 | バイオタイム インク．Ｂｉｏｔｉｍｅ Ｉｎｃ． | インビトロ及び生体内での軟骨形成のための方法及び組成物 |
| CN105496601A (zh) | 2010-10-21 | 2016-04-20 | 奥加诺沃公司 | 用于制造组织的装置、系统和方法 |
| US10865383B2 (en) | 2011-07-12 | 2020-12-15 | Lineage Cell Therapeutics, Inc. | Methods and formulations for orthopedic cell therapy |
| US9499779B2 (en) | 2012-04-20 | 2016-11-22 | Organovo, Inc. | Devices, systems, and methods for the fabrication of tissue utilizing UV cross-linking |
| US20140178343A1 (en) | 2012-12-21 | 2014-06-26 | Jian Q. Yao | Supports and methods for promoting integration of cartilage tissue explants |
| US9442105B2 (en) | 2013-03-15 | 2016-09-13 | Organovo, Inc. | Engineered liver tissues, arrays thereof, and methods of making the same |
| CA2919734C (fr) | 2013-07-31 | 2023-04-25 | Organovo, Inc. | Dispositifs, systemes et procedes automatises pour la fabrication de tissus |
| EP3808838B1 (fr) | 2014-04-04 | 2024-04-10 | Organovo, Inc. | Tissu mammaire tridimensionnel artificiel, tissu adipeux, et modèle de maladies tumorales |
| US9670443B2 (en) | 2014-06-25 | 2017-06-06 | University Of Leeds | Tissue engineered constructs |
| EP3204488B1 (fr) | 2014-10-06 | 2019-07-17 | Organovo, Inc. | Tissus rénaux produits par génie génétique, réseaux correspondants, et procédés de production |
| US20160122723A1 (en) | 2014-11-05 | 2016-05-05 | Organovo, Inc. | Engineered three-dimensional skin tissues, arrays thereof, and methods of making the same |
| WO2017083402A1 (fr) | 2015-11-09 | 2017-05-18 | Organovo, Inc. | Procédés améliorés de fabrication de tissus |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69631490T2 (de) * | 1995-11-09 | 2004-10-07 | Univ Massachusetts Boston | Wiederherstellung der gewebeoberfläche mit zusammensetzungen aus hydrogel-zellen |
| WO2000052145A2 (fr) * | 1999-03-02 | 2000-09-08 | University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus | Lignees cellulaires embryonnaires ou de type souche produites par transplantation nucleaire d'especes croisees |
| US7045353B2 (en) * | 2000-08-01 | 2006-05-16 | Yissum Research Development Company Of The Hebrew University Of Jerusalem | Directed differentiation of human embryonic cells |
| AU2002258477A1 (en) * | 2001-03-08 | 2002-09-24 | Advanced Cell Technology, Inc. | Use of rna interference for the creation of lineage specific es and other undifferentiated cells and production of differentiated cells in vitro by co-culture |
| EP1463800A4 (fr) * | 2001-12-07 | 2006-04-26 | Geron Corp | Precurseurs de chondrocytes derives de cellules souches embryonnaires humaines |
| US20020106625A1 (en) * | 2002-02-07 | 2002-08-08 | Hung Clark T. | Bioreactor for generating functional cartilaginous tissue |
| WO2003084385A2 (fr) * | 2002-04-02 | 2003-10-16 | William Marsh Rice University | Cellules redifferenciees utilisees pour reparer les defauts du cartilage |
| US20040082063A1 (en) * | 2002-10-18 | 2004-04-29 | Reliance Life Sciences Pvt. Ltd. | Tissue-like organization of cells and macroscopic tissue-like constructs, generated by macromass culture of cells, and the method of macromass culture |
| WO2006017176A2 (fr) * | 2004-07-09 | 2006-02-16 | William Marsh Rice University | Constructions sans echafaudage pour genie tissulaire de cartilage articulaire |
-
2007
- 2007-04-05 CA CA002648648A patent/CA2648648A1/fr not_active Abandoned
- 2007-04-05 WO PCT/US2007/066089 patent/WO2007115336A2/fr active Application Filing
- 2007-04-05 EP EP20070760209 patent/EP2007312A4/fr not_active Withdrawn
- 2007-04-05 EP EP07760205A patent/EP2007875A4/fr not_active Withdrawn
- 2007-04-05 CA CA002648332A patent/CA2648332A1/fr not_active Abandoned
- 2007-04-05 AU AU2007234365A patent/AU2007234365A1/en not_active Abandoned
- 2007-04-05 AU AU2007254048A patent/AU2007254048A1/en not_active Abandoned
- 2007-04-05 WO PCT/US2007/066092 patent/WO2007115337A2/fr active Application Filing
- 2007-04-05 AU AU2007234366A patent/AU2007234366A1/en not_active Abandoned
- 2007-04-05 WO PCT/US2007/066085 patent/WO2007136936A2/fr active Application Filing
- 2007-04-05 CA CA002648327A patent/CA2648327A1/fr not_active Abandoned
- 2007-04-05 EP EP07760211A patent/EP2007881A4/fr not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| AU2007234366A1 (en) | 2007-10-11 |
| EP2007875A2 (fr) | 2008-12-31 |
| WO2007115336A3 (fr) | 2008-05-22 |
| WO2007115337A2 (fr) | 2007-10-11 |
| EP2007881A4 (fr) | 2009-07-08 |
| AU2007234365A1 (en) | 2007-10-11 |
| WO2007115337A8 (fr) | 2007-12-13 |
| WO2007115337A3 (fr) | 2008-07-03 |
| EP2007312A2 (fr) | 2008-12-31 |
| EP2007312A4 (fr) | 2012-08-22 |
| WO2007115336A2 (fr) | 2007-10-11 |
| WO2007136936A2 (fr) | 2007-11-29 |
| WO2007136936A3 (fr) | 2008-10-23 |
| AU2007254048A1 (en) | 2007-11-29 |
| CA2648332A1 (fr) | 2007-10-11 |
| CA2648648A1 (fr) | 2007-11-29 |
| EP2007881A2 (fr) | 2008-12-31 |
| EP2007875A4 (fr) | 2009-12-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2648327A1 (fr) | Genie tissulaire a l'aide de cellules souches embryonnaires humaines | |
| Koay et al. | Tissue engineering with chondrogenically differentiated human embryonic stem cells | |
| Kang et al. | Effect of chondrocyte passage number on histological aspects of tissue-engineered cartilage | |
| EP3397294B1 (fr) | Procédé de préparation de bloc organoïde cartilagineux 3d | |
| Ibsirlioglu et al. | Decellularized biological scaffold and stem cells from autologous human adipose tissue for cartilage tissue engineering | |
| US20090142307A1 (en) | Shape-Based Approach for Scaffoldless Tissue Engineering | |
| US20090297579A1 (en) | Control of Cells and Cell Multipotentiality in Three Dimensional Matrices | |
| US9592255B2 (en) | Scaffold-free three dimensional nerve fibroblast constructs | |
| Van Osch et al. | Considerations on the use of ear chondrocytes as donor chondrocytes for cartilage tissue engineering | |
| Zhang et al. | The influence of scaffold microstructure on chondrogenic differentiation of mesenchymal stem cells | |
| US20090136559A1 (en) | Chondrocyte Differentiation from Human Embryonic Stem Cells and Their Use in Tissue Engineering | |
| EP3025720B1 (fr) | Procédé de préparation pour agent thérapeutique de chondrocyte du type bille | |
| Hoben et al. | Fibrochondrogenesis in two embryonic stem cell lines: effects of differentiation timelines | |
| US9422522B2 (en) | Method of producing adipocytes from fibroblast cells | |
| Schulz et al. | Cartilage tissue engineering by collagen matrix associated bone marrow derived mesenchymal stem cells | |
| Zhang et al. | The in vivo chondrogenesis of cartilage stem/progenitor cells from auricular cartilage and the perichondrium | |
| AU2010319502B2 (en) | Subpopulations of spore-like cells and uses thereof | |
| US11786636B2 (en) | Methods for complex tissue engineering | |
| US8637065B2 (en) | Dermis-derived cells for tissue engineering applications | |
| Khoshfetrat et al. | Seeding density modulates migration and morphology of rabbit chondrocytes cultured in collagen gels | |
| Bruschi et al. | Induced pluripotent stem cells–derived chondrocyte progenitors | |
| WO2014018459A1 (fr) | Compositions et procédés d'ingénierie biomédicale de cartilage | |
| Jessop et al. | 3D bioprinting cartilage | |
| Nishio et al. | Chondrocyte differentiation of human buccal fat pad-derived dedifferentiated fat cells and adipose stem cells using an atelocollagen sponge | |
| Hoben | Self assembly of fibrochondrocytes and human embryonic stem cells for tissue engineering of fibrocartilage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Dead |