CA2609150A1 - Compositions, methods and kits for real-time nucleic acid analysis in live cells - Google Patents

Compositions, methods and kits for real-time nucleic acid analysis in live cells Download PDF

Info

Publication number
CA2609150A1
CA2609150A1 CA002609150A CA2609150A CA2609150A1 CA 2609150 A1 CA2609150 A1 CA 2609150A1 CA 002609150 A CA002609150 A CA 002609150A CA 2609150 A CA2609150 A CA 2609150A CA 2609150 A1 CA2609150 A1 CA 2609150A1
Authority
CA
Canada
Prior art keywords
molecule
chimeric
nucleic acid
detector
peptide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002609150A
Other languages
English (en)
French (fr)
Inventor
James H. Eberwine
Ulo Langel
Emelia Eiriksdottir
Tiina Peritz
Jai-Yoon Sul
Philip G. Haydon
Junhyong Kim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pennsylvania
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2609150A1 publication Critical patent/CA2609150A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/127—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/72—Fusion polypeptide containing domain for protein-protein interaction containing SH2 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73—Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CA002609150A 2005-05-18 2006-05-17 Compositions, methods and kits for real-time nucleic acid analysis in live cells Abandoned CA2609150A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US68233405P 2005-05-18 2005-05-18
US60/682,334 2005-05-18
PCT/US2006/019107 WO2006125012A2 (en) 2005-05-18 2006-05-17 Compositions, methods and kits for real-time nucleic acid analysis in live cells

Publications (1)

Publication Number Publication Date
CA2609150A1 true CA2609150A1 (en) 2006-11-23

Family

ID=37432106

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002609150A Abandoned CA2609150A1 (en) 2005-05-18 2006-05-17 Compositions, methods and kits for real-time nucleic acid analysis in live cells

Country Status (6)

Country Link
US (2) US9260703B2 (enExample)
EP (1) EP1882047A4 (enExample)
JP (1) JP2008545394A (enExample)
AU (1) AU2006247215B2 (enExample)
CA (1) CA2609150A1 (enExample)
WO (1) WO2006125012A2 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0804495D0 (en) * 2008-03-11 2008-04-16 Iti Scotland Ltd Surface attachment
US8501405B2 (en) 2009-04-27 2013-08-06 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
WO2010144150A2 (en) 2009-06-12 2010-12-16 Pacific Biosciences Of California, Inc. Real-time analytical methods and systems
JP2020530267A (ja) 2017-06-30 2020-10-22 コデクシス， インコーポレイテッド Ｔ７ ｒｎａポリメラーゼバリアント
KR102773542B1 (ko) 2017-06-30 2025-02-25 코덱시스, 인코포레이티드 T7 rna 폴리머라제 변이체
EP4090747A1 (en) * 2020-01-17 2022-11-23 Sana Biotechnology, Inc. Safety switches for regulation of gene expression
WO2022084748A1 (en) * 2020-10-23 2022-04-28 Spindle Biotech, Inc. Compositions and methods for rna synthesis
WO2025038950A1 (en) * 2023-08-16 2025-02-20 Spotlight Therapeutics Cell-penetrating peptide compositions and uses thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0714980A1 (en) * 1994-12-02 1996-06-05 Institut Pasteur Hypermutagenesis
US6322901B1 (en) * 1997-11-13 2001-11-27 Massachusetts Institute Of Technology Highly luminescent color-selective nano-crystalline materials
US5990479A (en) * 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US6426513B1 (en) * 1998-09-18 2002-07-30 Massachusetts Institute Of Technology Water-soluble thiol-capped nanocrystals
US6251303B1 (en) * 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
AU2001250774B2 (en) * 2000-02-08 2005-01-27 Sangamo Biosciences, Inc. Cells expressing zinc finger proteins for drug discovery
US20020025317A1 (en) * 2000-07-20 2002-02-28 Schering Ag Bispecific monoclonal antibodies to IL-12 and IL-18
US6576291B2 (en) * 2000-12-08 2003-06-10 Massachusetts Institute Of Technology Preparation of nanocrystallites
WO2002086105A1 (en) * 2001-04-20 2002-10-31 Chiron Corporation Delivery of polynucleotide agents to the central nervous sysstem
JP2003061684A (ja) * 2001-06-11 2003-03-04 Nippon Genetech Co Ltd Ｄｎａの塩基配列決定法
JP2005504275A (ja) * 2001-09-18 2005-02-10 ユー．エス． ジェノミクス， インコーポレイテッド 高分解能線形解析用のポリマーの差示的タグ付け
JP2005533256A (ja) * 2002-07-17 2005-11-04 ユー．エス． ジェノミクス， インコーポレイテッド キメラタグを用いるポリマーを分析するための方法と組成物
US7343249B2 (en) * 2005-03-23 2008-03-11 International Business Machines Corporational Vectorization of sequence alignment computation using distance matrix reshaping

Also Published As

Publication number Publication date
WO2006125012A2 (en) 2006-11-23
US20160168558A1 (en) 2016-06-16
US10093917B2 (en) 2018-10-09
EP1882047A2 (en) 2008-01-30
AU2006247215A1 (en) 2006-11-23
JP2008545394A (ja) 2008-12-18
AU2006247215B2 (en) 2012-09-13
WO2006125012A3 (en) 2007-05-03
US20100041025A1 (en) 2010-02-18
EP1882047A4 (en) 2009-10-28
US9260703B2 (en) 2016-02-16

Similar Documents

Publication Publication Date Title
US10093917B2 (en) Compositions, methods and kits for real-time nucleic acid analysis in live cells
JP2011516026A (ja) 免疫関連疾患の治療のための組成物と方法
JP4969638B2 (ja) 単球由来核酸および関連する組成物ならびに方法
US6872558B1 (en) Heparanase-2 a member of the heparanase protein family
JP2003533977A (ja) 咽頭ガン関連タンパク質ｌａｒｃａｐ−１
US20030170717A1 (en) Detection of genes regulated by EGF in breast cancer
US20030032018A1 (en) Human smooth muscle myosin heavy chain
JP2021501601A (ja) コルヌリン（ｃｒｎｎ）バリアント及びその使用
US7094890B1 (en) Arthritis-associated protein
JP5587528B2 (ja) ｃ−Ｊｕｎ蛋白質と複合体を形成する蛋白質、及び、それをコードする核酸、ならびに、それらの利用方法
US7670787B2 (en) Protein forming complex with c-Fos protein, nucleic acid encoding the same and method of using the same
JP2003530122A (ja) 新規なセリン−トレオニンキナーゼ
JP7237064B2 (ja) 単一免疫グロブリンインターロイキン－１受容体関連（ｓｉｇｉｒｒ）変異型及びその使用
RU2815068C2 (ru) Варианты белка, родственного рецептору интерлейкина-1 и содержащего одиночный домен иммуноглобулина (sigirr), и их применение
JP2003525636A (ja) ヒトの細胞外シグナル制御キナーゼ
AU2002328896B2 (en) Diagnosis and treatment of diseases associated with altered expression of HIPK1
JP2003523758A (ja) 新規転写因子ｃａｒｐ−２
US8168752B2 (en) T cell proteins and nucleotides encoding the same
US7183397B2 (en) NK-2 homeobox transcription factor
WO2002052047A2 (en) Methods for identifying g-protein coupled receptors associated with diseases
JP2003523748A (ja) 新規ホスホジエステラーゼ７ｂ型
JP2020536500A (ja) 溶質キャリアファミリー１４メンバー１（ｓｌｃ１４ａ１）の変異型及びその使用
JP2008515797A (ja) オスの避妊標的物としてのｔｓｓｋファミリーメンバーおよびｔｓｋｓの評価
Maxwell Identification and characterization of GRP50: a novel Golgi-associated ankyrin repeat protein
JP2004505639A (ja) アポトーシスタンパク質のタンパク質阻害剤ｉａｐｌ−３

Legal Events

Date Code Title Description
EEER Examination request
FZDE Dead

Effective date: 20140106