AU2006247215B2 - Compositions, methods and kits for real-time nucleic acid analysis in live cells - Google Patents

Compositions, methods and kits for real-time nucleic acid analysis in live cells Download PDF

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Publication number
AU2006247215B2
AU2006247215B2 AU2006247215A AU2006247215A AU2006247215B2 AU 2006247215 B2 AU2006247215 B2 AU 2006247215B2 AU 2006247215 A AU2006247215 A AU 2006247215A AU 2006247215 A AU2006247215 A AU 2006247215A AU 2006247215 B2 AU2006247215 B2 AU 2006247215B2
Authority
AU
Australia
Prior art keywords
molecule
rna polymerase
detector
chimeric
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
AU2006247215A
Other languages
English (en)
Other versions
AU2006247215A1 (en
Inventor
James H. Eberwine
Emelia Eiriksdottir
Philip G. Haydon
Junhyong Kim
Ulo Langel
Tiina Peritz
Jai-Yoon Sul
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pennsylvania Penn
Original Assignee
University of Pennsylvania Penn
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Pennsylvania Penn filed Critical University of Pennsylvania Penn
Publication of AU2006247215A1 publication Critical patent/AU2006247215A1/en
Application granted granted Critical
Publication of AU2006247215B2 publication Critical patent/AU2006247215B2/en
Anticipated expiration legal-status Critical
Expired legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/127—RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/72—Fusion polypeptide containing domain for protein-protein interaction containing SH2 domain
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73—Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AU2006247215A 2005-05-18 2006-05-17 Compositions, methods and kits for real-time nucleic acid analysis in live cells Expired AU2006247215B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US68233405P 2005-05-18 2005-05-18
US60/682,334 2005-05-18
PCT/US2006/019107 WO2006125012A2 (en) 2005-05-18 2006-05-17 Compositions, methods and kits for real-time nucleic acid analysis in live cells

Publications (2)

Publication Number Publication Date
AU2006247215A1 AU2006247215A1 (en) 2006-11-23
AU2006247215B2 true AU2006247215B2 (en) 2012-09-13

Family

ID=37432106

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2006247215A Expired AU2006247215B2 (en) 2005-05-18 2006-05-17 Compositions, methods and kits for real-time nucleic acid analysis in live cells

Country Status (6)

Country Link
US (2) US9260703B2 (enExample)
EP (1) EP1882047A4 (enExample)
JP (1) JP2008545394A (enExample)
AU (1) AU2006247215B2 (enExample)
CA (1) CA2609150A1 (enExample)
WO (1) WO2006125012A2 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0804495D0 (en) * 2008-03-11 2008-04-16 Iti Scotland Ltd Surface attachment
WO2010129019A2 (en) 2009-04-27 2010-11-11 Pacific Biosciences Of California, Inc. Real-time sequencing methods and systems
WO2010144150A2 (en) 2009-06-12 2010-12-16 Pacific Biosciences Of California, Inc. Real-time analytical methods and systems
WO2019005540A1 (en) 2017-06-30 2019-01-03 Codexis, Inc. T7 POLYMERASE RNA VARIANTS
CN111417724A (zh) 2017-06-30 2020-07-14 科德克希思公司 T7 rna聚合酶变体
IL294820A (en) * 2020-01-17 2022-09-01 Sana Biotechnology Inc Safety switches for regulation of gene expression
CA3195982A1 (en) * 2020-10-23 2022-04-28 Spindle Biotech Inc. Compositions and methods for rna synthesis
WO2025038950A1 (en) * 2023-08-16 2025-02-20 Spotlight Therapeutics Cell-penetrating peptide compositions and uses thereof

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0714980A1 (en) * 1994-12-02 1996-06-05 Institut Pasteur Hypermutagenesis
US6322901B1 (en) 1997-11-13 2001-11-27 Massachusetts Institute Of Technology Highly luminescent color-selective nano-crystalline materials
US5990479A (en) 1997-11-25 1999-11-23 Regents Of The University Of California Organo Luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes
US6251303B1 (en) 1998-09-18 2001-06-26 Massachusetts Institute Of Technology Water-soluble fluorescent nanocrystals
US6426513B1 (en) 1998-09-18 2002-07-30 Massachusetts Institute Of Technology Water-soluble thiol-capped nanocrystals
DE60143192D1 (de) * 2000-02-08 2010-11-18 Sangamo Biosciences Inc Zellen zur entdeckung von medikamenten
US20020025317A1 (en) * 2000-07-20 2002-02-28 Schering Ag Bispecific monoclonal antibodies to IL-12 and IL-18
US6576291B2 (en) 2000-12-08 2003-06-10 Massachusetts Institute Of Technology Preparation of nanocrystallites
JP2004532849A (ja) * 2001-04-20 2004-10-28 カイロン コーポレイション 中枢神経系へのポリヌクレオチド薬剤の送達
JP2003061684A (ja) * 2001-06-11 2003-03-04 Nippon Genetech Co Ltd Ｄｎａの塩基配列決定法
AU2002336613A1 (en) * 2001-09-18 2003-04-01 U.S. Genomics, Inc. Differential tagging of polymers for high resolution linear analysis
WO2004007692A2 (en) * 2002-07-17 2004-01-22 U.S.Genomics, Inc. Methods and compositions for analyzing polymers using chimeric tags
US7343249B2 (en) * 2005-03-23 2008-03-11 International Business Machines Corporational Vectorization of sequence alignment computation using distance matrix reshaping

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
A. Ziemiecki et al. EMBO Journal (1990) Vol. 9, pp. 191-196. *
EA Ostrander et al. Science (1990) Vol.249, pp.1261-1265 *

Also Published As

Publication number Publication date
CA2609150A1 (en) 2006-11-23
WO2006125012A2 (en) 2006-11-23
EP1882047A2 (en) 2008-01-30
US20160168558A1 (en) 2016-06-16
US20100041025A1 (en) 2010-02-18
US9260703B2 (en) 2016-02-16
JP2008545394A (ja) 2008-12-18
US10093917B2 (en) 2018-10-09
AU2006247215A1 (en) 2006-11-23
EP1882047A4 (en) 2009-10-28
WO2006125012A3 (en) 2007-05-03

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