CA2589937C - Rhamnose promoter expression system - Google Patents

Rhamnose promoter expression system Download PDF

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Publication number
CA2589937C
CA2589937C CA2589937A CA2589937A CA2589937C CA 2589937 C CA2589937 C CA 2589937C CA 2589937 A CA2589937 A CA 2589937A CA 2589937 A CA2589937 A CA 2589937A CA 2589937 C CA2589937 C CA 2589937C
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CA
Canada
Prior art keywords
nucleic acid
sequence
acid sequence
vector
fab
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2589937A
Other languages
English (en)
French (fr)
Other versions
CA2589937A1 (en
Inventor
Johann Brass
Christoph Kiziak
Joachim Klein
Ralf Ostendorp
Armin Weidmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lonza AG
Morphosys AG
Original Assignee
Lonza AG
Morphosys AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza AG, Morphosys AG filed Critical Lonza AG
Publication of CA2589937A1 publication Critical patent/CA2589937A1/en
Application granted granted Critical
Publication of CA2589937C publication Critical patent/CA2589937C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CA2589937A 2004-12-07 2005-12-05 Rhamnose promoter expression system Active CA2589937C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP04028917 2004-12-07
EP04028917.5 2004-12-07
PCT/EP2005/013013 WO2006061174A2 (en) 2004-12-07 2005-12-05 Rhamnose promoter expression system

Publications (2)

Publication Number Publication Date
CA2589937A1 CA2589937A1 (en) 2006-06-15
CA2589937C true CA2589937C (en) 2014-04-22

Family

ID=36304177

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2589937A Active CA2589937C (en) 2004-12-07 2005-12-05 Rhamnose promoter expression system

Country Status (14)

Country Link
US (3) US20080206817A1 (zh-CN)
EP (2) EP1824980B1 (zh-CN)
JP (1) JP5069124B2 (zh-CN)
CN (1) CN101090969B (zh-CN)
AT (1) ATE466090T1 (zh-CN)
AU (1) AU2005313531B2 (zh-CN)
CA (1) CA2589937C (zh-CN)
DE (1) DE602005020972D1 (zh-CN)
DK (1) DK1824980T3 (zh-CN)
ES (1) ES2346894T3 (zh-CN)
HK (1) HK1115156A1 (zh-CN)
IL (1) IL183608A (zh-CN)
SG (1) SG149071A1 (zh-CN)
WO (1) WO2006061174A2 (zh-CN)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8067179B2 (en) * 2006-11-30 2011-11-29 Research Development Foundation Immunoglobulin libraries
WO2009025888A2 (en) 2007-05-10 2009-02-26 The Arizona Board Of Regents For And On Behalf Of Arizona State University Regulated synthesis of antigen and/or regulated attentuation to enhance vaccine immunogenics and/or safety
ES2381433T3 (es) 2008-02-29 2012-05-28 Xbrane Bioscience Aktiebolag Sistema de expresión para proteínas
CN102978219B (zh) * 2012-11-09 2015-05-13 汕头大学 一种弧菌交叉保护性抗原及其制备方法和应用
US9911245B1 (en) * 2013-07-19 2018-03-06 Geotoll, Inc. Method and apparatus for using a vehicle license tag number for toll payment as a backup form of account authorization
CN106676125B (zh) * 2015-11-05 2020-08-14 中国科学院天津工业生物技术研究所 一种包含麦芽糖启动子的载体以及麦芽糖启动子突变体
CN109219662A (zh) 2016-03-02 2019-01-15 龙沙有限公司 改进的发酵工艺
EP3444269A1 (en) 2017-08-17 2019-02-20 National Research Council of Canada Systems and methods for the production of diphtheria toxin polypeptides
US20230374526A1 (en) 2020-03-26 2023-11-23 Asklepios Biopharmaceutical, Inc. Forskolin-inducible promoters and hypoxia-inducible promoters
EP4136237A1 (en) 2020-04-15 2023-02-22 Asklepios Biopharmaceutical, Inc. Forskolin-inducible promoters and hypoxia-inducible promoters
EP3981879A1 (en) * 2020-10-09 2022-04-13 Stada Arzneimittel Ag Method of producing a recombinant protein in a host cell which has a disabled rhamnose metabolism as well as expression vectors, host cells and recombinant proteins thereof
AU2022349814A1 (en) * 2021-09-24 2024-05-02 Xbrane Biopharma Ab Dna constructs and host cells for expressing recombinant protein

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR870700095A (ko) 1985-01-28 1987-02-28 원본미기재 AraB 프로모터 및 미생물학적 방법에 의해 세크로핀을 포함한 폴리펩타이드를 제조하는 방법
DE68926882T2 (de) * 1988-01-11 1997-02-13 Xoma Corp Plasmidvektor mit pectatlyase-signalsequenz
US5401629A (en) 1990-08-07 1995-03-28 The Salk Institute Biotechnology/Industrial Associates, Inc. Assay methods and compositions useful for measuring the transduction of an intracellular signal
US5240831A (en) * 1991-01-10 1993-08-31 Board Of Regents, The University Of Texas Methods and compositions for the expression of biologically active eukaryotic cytochrome p45os in bacteria
US5435128A (en) * 1993-11-15 1995-07-25 Thiokol Corporation Pyrotechnically driven nozzle restrictor
WO1998002462A1 (en) 1996-07-16 1998-01-22 Morphosys Gesellschaft Für Proteinoptimierung Mbh Immunoglobulin superfamily domains and fragments with increased solubility
US7094579B2 (en) 2002-02-13 2006-08-22 Xoma Technology Ltd. Eukaryotic signal sequences for prokaryotic expression
AU2003288189B8 (en) * 2002-12-02 2009-06-25 Basf Aktiengesellschaft L-rhamnose-inducible expression systems
CA2513025A1 (en) * 2003-01-09 2004-07-29 Macrogenics, Inc. Dual expression vector system for antibody expression in bacterial and mammalian cells

Also Published As

Publication number Publication date
US20120135463A1 (en) 2012-05-31
DE602005020972D1 (en) 2010-06-10
ES2346894T3 (es) 2010-10-21
US20170107525A1 (en) 2017-04-20
WO2006061174A2 (en) 2006-06-15
CN101090969B (zh) 2013-01-16
CA2589937A1 (en) 2006-06-15
SG149071A1 (en) 2009-01-29
CN101090969A (zh) 2007-12-19
IL183608A0 (en) 2007-09-20
JP5069124B2 (ja) 2012-11-07
EP2123761A3 (en) 2009-12-16
EP1824980A2 (en) 2007-08-29
US9279128B2 (en) 2016-03-08
JP2008522599A (ja) 2008-07-03
AU2005313531A1 (en) 2006-06-15
DK1824980T3 (da) 2010-08-16
EP2123761A2 (en) 2009-11-25
US20080206817A1 (en) 2008-08-28
IL183608A (en) 2011-10-31
ATE466090T1 (de) 2010-05-15
WO2006061174A3 (en) 2007-01-18
HK1115156A1 (en) 2008-12-24
EP1824980B1 (en) 2010-04-28
AU2005313531B2 (en) 2011-04-14

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