CA2585404A1 - Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms - Google Patents

Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms Download PDF

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Publication number
CA2585404A1
CA2585404A1 CA002585404A CA2585404A CA2585404A1 CA 2585404 A1 CA2585404 A1 CA 2585404A1 CA 002585404 A CA002585404 A CA 002585404A CA 2585404 A CA2585404 A CA 2585404A CA 2585404 A1 CA2585404 A1 CA 2585404A1
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CA
Canada
Prior art keywords
seq
gene
sequence
nucleic acid
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002585404A
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English (en)
French (fr)
Inventor
Narendra S. Yadav
Zhixiong Xue
Hongxiang Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2585404A1 publication Critical patent/CA2585404A1/en
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6458—Glycerides by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
  • Edible Oils And Fats (AREA)
CA002585404A 2004-11-04 2005-11-03 Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms Abandoned CA2585404A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US62481204P 2004-11-04 2004-11-04
US60/624,812 2004-11-04
US11/024,544 US7273746B2 (en) 2004-11-04 2004-12-29 Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US11/024,544 2004-12-29
PCT/US2005/040345 WO2006052914A2 (en) 2004-11-04 2005-11-03 Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms

Publications (1)

Publication Number Publication Date
CA2585404A1 true CA2585404A1 (en) 2006-05-18

Family

ID=36262489

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002585404A Abandoned CA2585404A1 (en) 2004-11-04 2005-11-03 Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms

Country Status (5)

Country Link
US (3) US7273746B2 (https=)
EP (1) EP1807525A4 (https=)
JP (1) JP2008524984A (https=)
CA (1) CA2585404A1 (https=)
WO (2) WO2006052914A2 (https=)

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US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
CN101578363A (zh) 2006-08-29 2009-11-11 联邦科学技术研究组织 脂肪酸的合成
US7709239B2 (en) * 2006-12-07 2010-05-04 E.I. Du Pont De Nemours And Company Mutant Δ8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
EP2147109B1 (en) * 2007-05-24 2014-03-19 E. I. Du Pont de Nemours and Company Dgat genes from yarrowia lipolytica for increased seed storage lipid production and altered fatty acid profiles in soybean
CA2701130A1 (en) * 2007-10-03 2009-04-09 E.I. Du Pont De Nemours And Company Peroxisome biogenesis factor protein (pex) disruptions for altering the content of polyunsaturated fatty acids and the total lipid content in oleaginous eukaryotic organisms
EP2620501A3 (en) * 2008-05-23 2013-10-02 E. I. du Pont de Nemours and Company DGAT genes from oleaginous organisms for increased seed storage lipid production and altered fatty acid profiles in oilseed plants
BRPI0908694A2 (pt) 2008-05-23 2019-09-24 Du Pont ''sementes de grão de soja transgência e método para aumentar o teor de ácido graxo total de uma semente de grão de soja''
WO2010025374A2 (en) 2008-08-29 2010-03-04 E. I. Du Pont De Nemours And Company Manipulation of snf1 protein kinase activity for altered oil content in oleaginous organisms
NO2358882T3 (https=) 2008-11-18 2017-12-23
US8431772B1 (en) 2008-11-19 2013-04-30 University Of Kentucky Research Foundation Diacylglycerol acyltransferase sequences and related methods
EP2504441B1 (en) 2009-11-23 2020-07-22 E. I. du Pont de Nemours and Company Sucrose transporter genes for increasing plant seed lipids
RU2514655C2 (ru) 2009-12-21 2014-04-27 Сантори Холдингз Лимитед Гены диацилглицерол-ацилтрансферазы и их использование
EP2635663B1 (en) 2010-11-03 2019-05-08 Corbion Biotech, Inc. Microbial oils with lowered pour points, dielectric fluids produced therefrom, and related methods
EP2691518A1 (en) 2011-03-31 2014-02-05 E.I. Du Pont De Nemours And Company Yarrowia diacylglycerol acyltransferase promoter regions for gene expression in yeast
CA2833400A1 (en) 2011-05-26 2012-11-29 E.I. Dupont De Nemours And Company Expression of caleosin in recombinant oleaginous microorganisms to increase oil content therein
JP6294827B2 (ja) * 2011-10-19 2018-03-14 マサチューセッツ インスティテュート オブ テクノロジー 操作された微生物および微生物油生成のための方法
CA2860220C (en) 2011-12-22 2021-07-13 E. I. Du Pont De Nemours And Company Use of the soybean or medicago truncatula sucrose synthase promoter to increase plant seed oil content
PL2861059T3 (pl) 2012-06-15 2017-10-31 Commw Scient Ind Res Org Wytwarzanie długołańcuchowych wielonienasyconych kwasów tłuszczowych w komórkach roślinnych
MX2015001780A (es) 2012-08-10 2015-09-28 Opx Biotechnologies Inc Microorganismos y métodos para la producción de ácidos grasos y productos derivados de ácidos grasos.
US9328336B2 (en) 2012-10-16 2016-05-03 Exxonmobil Research And Engineering Company DGAT genes and methods of use for triglyceride production in recombinant microorganisms
NZ706832A (en) 2012-10-30 2019-04-26 Agresearch Ltd Enhanced acyltransferase polynucleotides, polypeptides, and methods of use
AU2013340445B2 (en) 2012-10-30 2019-08-22 New Zealand Institute For Bioeconomy Science Limited Improved acyltransferase polynucleotides, polypeptides, and methods of use
MX359167B (es) * 2012-10-30 2018-09-18 Agresearch Ltd Polinucleotidos, polipeptidos y metodos de uso novedosos de aciltransferasa.
BR112015012811A2 (pt) 2012-12-06 2017-11-14 Exxonmobil Res & Eng Co genes dgat compreendendo domínios de homologia de plecstrina e uso em micro-organismos recombinantes
CN109576241A (zh) 2012-12-21 2019-04-05 新西兰植物和食品研究院有限公司 基因表达的调控
US20150119601A1 (en) 2013-03-15 2015-04-30 Opx Biotechnologies, Inc. Monofunctional mcr + 3-hp dehydrogenase
EP3022310B1 (en) 2013-07-19 2019-10-16 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
US11408013B2 (en) 2013-07-19 2022-08-09 Cargill, Incorporated Microorganisms and methods for the production of fatty acids and fatty acid derived products
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DK3110947T3 (da) * 2014-02-24 2019-11-04 Novogy Inc Diacylglycerolacyltransferase (dga1)-polynukleotider og fremgangsmåder til forøgelse af gærcellelipidfremstilling ved hjælp af overekspression af heterolog dga1
WO2015164457A1 (en) 2014-04-22 2015-10-29 E. I. Du Pont De Nemours And Company Plastidic carbonic anhydrase genes for oil augmentation in seeds with increased dgat expression
PH12016502586B1 (en) 2014-06-27 2023-07-19 Commw Scient Ind Res Org Lipid comprising docosapentaenoic acid
EP2993228B1 (en) 2014-09-02 2019-10-09 Cargill, Incorporated Production of fatty acid esters
AU2017322897B9 (en) * 2016-09-07 2022-06-09 Kao Corporation Method of producing lipid
US11396657B2 (en) 2017-01-27 2022-07-26 Regents Of The University Of Minnesota Plants having increased oil quality
US11345938B2 (en) 2017-02-02 2022-05-31 Cargill, Incorporated Genetically modified cells that produce C6-C10 fatty acid derivatives
US11337391B2 (en) 2017-08-18 2022-05-24 Regents Of The University Of Minnesota Early flowering plants
US11707029B2 (en) 2017-08-18 2023-07-25 Regents Of The University Of Minnesota Oilseed plants having reduced pod shatter
WO2019157504A1 (en) 2018-02-12 2019-08-15 Regents Of The University Of Minnesota Plants having increased oil quality
CN109837256B (zh) * 2019-03-21 2020-12-01 江南大学 一种二酰甘油酰基转移酶1及其在生产甘油三酯中的应用
US11408008B2 (en) * 2019-03-28 2022-08-09 Regents Of The University Of Minnesota Plants having increased oil quality
CN114517164A (zh) * 2020-11-19 2022-05-20 中国科学院青岛生物能源与过程研究所 一种脂肪酸延长酶基因在酵母合成神经酸中的应用
CA3210860A1 (en) * 2021-03-03 2022-09-09 Anna EL TAHCHY Production of phospholipids in microbes and uses thereof
CN116396875B (zh) * 2022-12-20 2026-04-17 华东理工大学 一种解脂耶氏酵母基因工程菌及其应用

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Also Published As

Publication number Publication date
US8685681B2 (en) 2014-04-01
WO2006052914A3 (en) 2009-02-19
WO2006052914A2 (en) 2006-05-18
US20060094086A1 (en) 2006-05-04
US20090104674A1 (en) 2009-04-23
JP2008524984A (ja) 2008-07-17
EP1807525A4 (en) 2010-02-17
WO2006052912A2 (en) 2006-05-18
EP1807525A2 (en) 2007-07-18
US20080020415A1 (en) 2008-01-24
WO2006052912A3 (en) 2009-04-09
US7273746B2 (en) 2007-09-25

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued

Effective date: 20141003