CA2580009C - Host-vector system for antibiotic-free cole1 plasmid propagation - Google Patents

Host-vector system for antibiotic-free cole1 plasmid propagation Download PDF

Info

Publication number
CA2580009C
CA2580009C CA 2580009 CA2580009A CA2580009C CA 2580009 C CA2580009 C CA 2580009C CA 2580009 CA2580009 CA 2580009 CA 2580009 A CA2580009 A CA 2580009A CA 2580009 C CA2580009 C CA 2580009C
Authority
CA
Canada
Prior art keywords
plasmid
rna
gene
protein
dna sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA 2580009
Other languages
English (en)
French (fr)
Other versions
CA2580009A1 (en
Inventor
Reingard Grabherr
Irene Pfaffenzeller
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim RCV GmbH and Co KG
Original Assignee
Boehringer Ingelheim RCV GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim RCV GmbH and Co KG filed Critical Boehringer Ingelheim RCV GmbH and Co KG
Publication of CA2580009A1 publication Critical patent/CA2580009A1/en
Application granted granted Critical
Publication of CA2580009C publication Critical patent/CA2580009C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA 2580009 2004-09-17 2005-09-08 Host-vector system for antibiotic-free cole1 plasmid propagation Expired - Lifetime CA2580009C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP04022201.0 2004-09-17
EP04022201 2004-09-17
PCT/EP2005/054450 WO2006029985A2 (en) 2004-09-17 2005-09-08 Host-vector system for antibiotic-free cole1 plasmid propagation

Publications (2)

Publication Number Publication Date
CA2580009A1 CA2580009A1 (en) 2006-03-23
CA2580009C true CA2580009C (en) 2013-12-17

Family

ID=34926585

Family Applications (1)

Application Number Title Priority Date Filing Date
CA 2580009 Expired - Lifetime CA2580009C (en) 2004-09-17 2005-09-08 Host-vector system for antibiotic-free cole1 plasmid propagation

Country Status (13)

Country Link
US (2) US20060063232A1 (enExample)
EP (1) EP1794299B1 (enExample)
JP (1) JP5301832B2 (enExample)
KR (2) KR101023066B1 (enExample)
CN (2) CN103555644B (enExample)
AR (2) AR050649A1 (enExample)
AU (1) AU2005284136B2 (enExample)
BR (1) BRPI0515436A (enExample)
CA (1) CA2580009C (enExample)
IL (1) IL181919A (enExample)
NZ (1) NZ554236A (enExample)
TW (1) TWI311152B (enExample)
WO (1) WO2006029985A2 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0327056D0 (en) * 2003-11-20 2003-12-24 Cobra Biolog Ltd Plasmid maintenance
AU2008252990B2 (en) 2007-05-17 2015-01-15 Boehringer Ingelheim Rcv Gmbh & Co Kg Method for producing a recombinant protein on a manufacturing scale
EP2152889B1 (en) * 2007-05-29 2017-03-15 Nature Technology Corporation Vectors and methods for genetic immunization
EP2173879A4 (en) * 2007-07-16 2010-10-20 Royal Veterinary College GEN / INHIBITOR COMBINATIONS WITH MUTUAL SUPPRESSION FOR THE ANTIBIOTIC-FREE SELECTION OF RECOMBINANT STRAINS
JP5538691B2 (ja) * 2007-11-22 2014-07-02 独立行政法人科学技術振興機構 Ｒｎａ−蛋白質相互作用モチーフを利用した蛋白質応答翻訳制御システム
EP2500427B1 (en) 2007-11-22 2014-07-30 Japan Science and Technology Agency Translation regulation system in cell or artifical cell model by using low-molecular-weight RNA
HUE031597T2 (en) * 2009-05-22 2017-07-28 Merial Inc Antibiotic-free plasmid
US20110269184A1 (en) 2009-07-16 2011-11-03 Boehringer Ingelheim Rcv Gmbh & Co. Kg Method for controlling plasmid copy number in e.coli
WO2012082946A2 (en) * 2010-12-14 2012-06-21 The Regents Of The University Of California Creation of super-stable coie1 plasmids by duplication of sl 1-4 sequence and point mutations.
GB201021795D0 (en) * 2010-12-21 2011-02-02 Msd Biolog Uk Ltd Expression Process
EP2890404B1 (en) 2012-08-29 2018-06-13 Nature Technology Corporation Dna plasmids with improved expression
WO2014077863A1 (en) 2012-11-19 2014-05-22 Nature Technology Corporation Replicative minicircle vectors with improved expression
JP7161991B2 (ja) * 2016-11-02 2022-10-27 アーチャーディーエックス， エルエルシー 免疫レパートリーシーケンシングのための核酸サンプル調製の方法
CN107142272A (zh) * 2017-06-05 2017-09-08 南京金斯瑞生物科技有限公司 一种控制大肠杆菌中质粒复制的方法
DK3456821T4 (da) 2017-09-19 2024-04-15 Deutsches Krebsforsch Ikke-integrerende dna-vektorer til genetisk modifikation af celler
WO2019057774A1 (en) 2017-09-19 2019-03-28 Deutsches Krebsforschungszentrum NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS
JP7520719B2 (ja) 2018-03-21 2024-07-23 アルデブロン，エル．エル．シー． 生成が改良されたウイルス及び非ウイルスのナノプラスミドベクター
JP7661015B2 (ja) 2020-06-12 2025-04-14 ナガセヴィータ株式会社 目的タンパク質の製造方法
WO2023166132A1 (en) * 2022-03-04 2023-09-07 Gen-H Genetic Engineering Heidelberg Gmbh Microorganism strain for antibiotic-free plasmid-based fermentation and method for generation thereof
CN117187281A (zh) * 2023-09-11 2023-12-08 云舟生物科技(广州)股份有限公司 一种无抗性质粒生产系统及其应用
CN118028194B (zh) * 2023-12-26 2024-09-06 浙江大学 基因组精简的恶臭假单胞菌菌株
WO2025160245A1 (en) 2024-01-25 2025-07-31 Aldevron, L.L.C. Viral and non-viral nanoplasmid vectors with improved production
WO2025231315A2 (en) 2024-05-03 2025-11-06 Aldevron, L.L.C. Bacterial host strains

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9215541D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
US5922583A (en) * 1995-10-17 1999-07-13 Biostar Inc. Methods for production of recombinant plasmids
EP1195436A1 (en) 2000-10-04 2002-04-10 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Expression vectors with modified ColE1 origin of replication for control of plasmid copy number
US20040248083A1 (en) * 2001-05-17 2004-12-09 Mikkelsen Jacob Giehm Vectors for gene therapy
SE0102204D0 (sv) * 2001-06-21 2001-06-21 Leif Isaksson New method
CN1490056A (zh) * 2002-10-18 2004-04-21 ��¡���ɵ°��̲��о����� 针对hiv-1的免疫方法和组合物
GB0327056D0 (en) 2003-11-20 2003-12-24 Cobra Biolog Ltd Plasmid maintenance

Also Published As

Publication number Publication date
IL181919A (en) 2012-04-30
AR050649A1 (es) 2006-11-08
AU2005284136B2 (en) 2011-07-14
TWI311152B (en) 2009-06-21
TW200621985A (en) 2006-07-01
KR101023066B1 (ko) 2011-03-24
AU2005284136A1 (en) 2006-03-23
CN103555644A (zh) 2014-02-05
US20090263861A1 (en) 2009-10-22
KR20100101689A (ko) 2010-09-17
EP1794299A2 (en) 2007-06-13
KR20070059130A (ko) 2007-06-11
NZ554236A (en) 2010-05-28
WO2006029985A3 (en) 2006-07-27
EP1794299B1 (en) 2015-09-02
CN101052722A (zh) 2007-10-10
US9297014B2 (en) 2016-03-29
BRPI0515436A (pt) 2008-07-22
AR079958A2 (es) 2012-02-29
JP2008512998A (ja) 2008-05-01
US20060063232A1 (en) 2006-03-23
IL181919A0 (en) 2007-07-04
CN103555644B (zh) 2018-06-01
JP5301832B2 (ja) 2013-09-25
WO2006029985A2 (en) 2006-03-23
CA2580009A1 (en) 2006-03-23

Similar Documents

Publication Publication Date Title
US9297014B2 (en) Host-vector system antibiotic-free ColE1 plasmid propagation
Opdyke et al. RNase III participates in GadY-dependent cleavage of the gadX-gadW mRNA
Williams et al. Generic plasmid DNA production platform incorporating low metabolic burden seed‐stock and fed‐batch fermentation processes
US11618899B2 (en) Cloning and expression vectors and systems
KR102688555B1 (ko) CRISPR/Cpf1 시스템을 기반으로 한 유전체 단일 염기 편집 방법 및 이의 용도
PL222067B1 (pl) Kaseta ekspresyjna, zastosowanie kasety ekspresyjnej, wektor ekspresyjny, komórka prokariotycznego gospodarza zawierająca wektor ekspresyjny, szczep bakteryjny oraz sposób wytwarzania polipeptydu
US20030003525A1 (en) Novel promoter of gene expression
US20110269184A1 (en) Method for controlling plasmid copy number in e.coli
JP2007151542A (ja) 相同組換えによる目的遺伝子のクローニング及び発現方法
JP2025507940A (ja) 抗生物質を含まないプラスミドベースの発酵のための微生物株及びその生成方法
MX2007003110A (en) Host-vector system for antibiotic-free cole1 plasmid propagation
Balbás et al. Molecular cloning by plasmid vectors
EP1706490A2 (en) Systems for tightly regulated gene expression
Zh et al. Design and study on characteristics of auto-and smoothly regulated genetic element O3/PlacUV5/Olac→ lacI
US20100167358A1 (en) Compositions and processes for improved plasmid dna production
RU2312146C1 (ru) Вектор на основе репликона бактериофага n15 и рекомбинантный вектор для регулируемой экспрессии целевого гена в клетках escherichia coli, штамм escherichia coli, обеспечивающий возможность регуляции числа копий вектора, и система экспрессии
KR20250042179A (ko) CRISPR-Cas9-기반의 플라스미드 DNA 주형의 선형화
CN1989255A (zh) 降低细胞中的自发突变率
Geist et al. TraM protein of plasmid R1: in vitro selection of the target region reveals two consensus 7 bp binding motifs spaced by a 4 bp linker of defined sequence
CN120400098A (zh) 一种Cas12核酸酶突变体及其应用
Zolotukhina et al. Mutational Analysis of a Leader Region of the Escherichia coli ribB Gene
HK1086297A1 (zh) 具有条件性复制起点的环形dna分子及其制备方法
HK1086297B (en) Circular dna molecule having a conditional origin of replication and process for preparation

Legal Events

Date Code Title Description
EEER Examination request