CN103555644B - 用于无抗生素ColE1 质粒增殖的宿主-载体系统 - Google Patents

用于无抗生素ColE1 质粒增殖的宿主-载体系统 Download PDF

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Publication number
CN103555644B
CN103555644B CN201310409070.8A CN201310409070A CN103555644B CN 103555644 B CN103555644 B CN 103555644B CN 201310409070 A CN201310409070 A CN 201310409070A CN 103555644 B CN103555644 B CN 103555644B
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plasmid
rna
cell
host
gene
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CN103555644A (zh
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莱因加德.格拉贝尔
艾琳.普法芬泽勒
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Boehringer Ingelheim RCV GmbH and Co KG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201310409070.8A 2004-09-17 2005-09-08 用于无抗生素ColE1 质粒增殖的宿主-载体系统 Expired - Lifetime CN103555644B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP04022201.0 2004-09-17
EP04022201 2004-09-17
CNA2005800377789A CN101052722A (zh) 2004-09-17 2005-09-08 用于无抗生素ColE1质粒增殖的宿主－载体系统

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CNA2005800377789A Division CN101052722A (zh) 2004-09-17 2005-09-08 用于无抗生素ColE1质粒增殖的宿主－载体系统

Publications (2)

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CN103555644A CN103555644A (zh) 2014-02-05
CN103555644B true CN103555644B (zh) 2018-06-01

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CN201310409070.8A Expired - Lifetime CN103555644B (zh) 2004-09-17 2005-09-08 用于无抗生素ColE1 质粒增殖的宿主-载体系统
CNA2005800377789A Pending CN101052722A (zh) 2004-09-17 2005-09-08 用于无抗生素ColE1质粒增殖的宿主－载体系统

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US (2) US20060063232A1 (enExample)
EP (1) EP1794299B1 (enExample)
JP (1) JP5301832B2 (enExample)
KR (2) KR101023066B1 (enExample)
CN (2) CN103555644B (enExample)
AR (2) AR050649A1 (enExample)
AU (1) AU2005284136B2 (enExample)
BR (1) BRPI0515436A (enExample)
CA (1) CA2580009C (enExample)
IL (1) IL181919A (enExample)
NZ (1) NZ554236A (enExample)
TW (1) TWI311152B (enExample)
WO (1) WO2006029985A2 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0327056D0 (en) * 2003-11-20 2003-12-24 Cobra Biolog Ltd Plasmid maintenance
AU2008252990B2 (en) 2007-05-17 2015-01-15 Boehringer Ingelheim Rcv Gmbh & Co Kg Method for producing a recombinant protein on a manufacturing scale
EP2152889B1 (en) * 2007-05-29 2017-03-15 Nature Technology Corporation Vectors and methods for genetic immunization
EP2173879A4 (en) * 2007-07-16 2010-10-20 Royal Veterinary College GEN / INHIBITOR COMBINATIONS WITH MUTUAL SUPPRESSION FOR THE ANTIBIOTIC-FREE SELECTION OF RECOMBINANT STRAINS
JP5538691B2 (ja) * 2007-11-22 2014-07-02 独立行政法人科学技術振興機構 Ｒｎａ−蛋白質相互作用モチーフを利用した蛋白質応答翻訳制御システム
EP2500427B1 (en) 2007-11-22 2014-07-30 Japan Science and Technology Agency Translation regulation system in cell or artifical cell model by using low-molecular-weight RNA
HUE031597T2 (en) * 2009-05-22 2017-07-28 Merial Inc Antibiotic-free plasmid
US20110269184A1 (en) 2009-07-16 2011-11-03 Boehringer Ingelheim Rcv Gmbh & Co. Kg Method for controlling plasmid copy number in e.coli
WO2012082946A2 (en) * 2010-12-14 2012-06-21 The Regents Of The University Of California Creation of super-stable coie1 plasmids by duplication of sl 1-4 sequence and point mutations.
GB201021795D0 (en) * 2010-12-21 2011-02-02 Msd Biolog Uk Ltd Expression Process
EP2890404B1 (en) 2012-08-29 2018-06-13 Nature Technology Corporation Dna plasmids with improved expression
WO2014077863A1 (en) 2012-11-19 2014-05-22 Nature Technology Corporation Replicative minicircle vectors with improved expression
JP7161991B2 (ja) * 2016-11-02 2022-10-27 アーチャーディーエックス， エルエルシー 免疫レパートリーシーケンシングのための核酸サンプル調製の方法
CN107142272A (zh) * 2017-06-05 2017-09-08 南京金斯瑞生物科技有限公司 一种控制大肠杆菌中质粒复制的方法
DK3456821T4 (da) 2017-09-19 2024-04-15 Deutsches Krebsforsch Ikke-integrerende dna-vektorer til genetisk modifikation af celler
WO2019057774A1 (en) 2017-09-19 2019-03-28 Deutsches Krebsforschungszentrum NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS
JP7520719B2 (ja) 2018-03-21 2024-07-23 アルデブロン，エル．エル．シー． 生成が改良されたウイルス及び非ウイルスのナノプラスミドベクター
JP7661015B2 (ja) 2020-06-12 2025-04-14 ナガセヴィータ株式会社 目的タンパク質の製造方法
WO2023166132A1 (en) * 2022-03-04 2023-09-07 Gen-H Genetic Engineering Heidelberg Gmbh Microorganism strain for antibiotic-free plasmid-based fermentation and method for generation thereof
CN117187281A (zh) * 2023-09-11 2023-12-08 云舟生物科技(广州)股份有限公司 一种无抗性质粒生产系统及其应用
CN118028194B (zh) * 2023-12-26 2024-09-06 浙江大学 基因组精简的恶臭假单胞菌菌株
WO2025160245A1 (en) 2024-01-25 2025-07-31 Aldevron, L.L.C. Viral and non-viral nanoplasmid vectors with improved production
WO2025231315A2 (en) 2024-05-03 2025-11-06 Aldevron, L.L.C. Bacterial host strains

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9215541D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
US5922583A (en) * 1995-10-17 1999-07-13 Biostar Inc. Methods for production of recombinant plasmids
EP1195436A1 (en) 2000-10-04 2002-04-10 BOEHRINGER INGELHEIM INTERNATIONAL GmbH Expression vectors with modified ColE1 origin of replication for control of plasmid copy number
US20040248083A1 (en) * 2001-05-17 2004-12-09 Mikkelsen Jacob Giehm Vectors for gene therapy
SE0102204D0 (sv) * 2001-06-21 2001-06-21 Leif Isaksson New method
CN1490056A (zh) * 2002-10-18 2004-04-21 ��¡���ɵ°��̲��о����� 针对hiv-1的免疫方法和组合物
GB0327056D0 (en) 2003-11-20 2003-12-24 Cobra Biolog Ltd Plasmid maintenance

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Produktion resistenzfreier plasmide für den therapeutischen einsatz von DNA im bereich der humanmedizin;Silvia Pichler;《Durchgeführt am Institut für angewandte mikrobiologie der universität für bodenkultur wien》;20020831;第2.1节，第2.2节，第5部分即讨论部分 *

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IL181919A (en) 2012-04-30
CA2580009C (en) 2013-12-17
AR050649A1 (es) 2006-11-08
AU2005284136B2 (en) 2011-07-14
TWI311152B (en) 2009-06-21
TW200621985A (en) 2006-07-01
KR101023066B1 (ko) 2011-03-24
AU2005284136A1 (en) 2006-03-23
CN103555644A (zh) 2014-02-05
US20090263861A1 (en) 2009-10-22
KR20100101689A (ko) 2010-09-17
EP1794299A2 (en) 2007-06-13
KR20070059130A (ko) 2007-06-11
NZ554236A (en) 2010-05-28
WO2006029985A3 (en) 2006-07-27
EP1794299B1 (en) 2015-09-02
CN101052722A (zh) 2007-10-10
US9297014B2 (en) 2016-03-29
BRPI0515436A (pt) 2008-07-22
AR079958A2 (es) 2012-02-29
JP2008512998A (ja) 2008-05-01
US20060063232A1 (en) 2006-03-23
IL181919A0 (en) 2007-07-04
JP5301832B2 (ja) 2013-09-25
WO2006029985A2 (en) 2006-03-23
CA2580009A1 (en) 2006-03-23

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