CA2576804C - A method of analysing a sample and apparatus therefor - Google Patents

A method of analysing a sample and apparatus therefor Download PDF

Info

Publication number
CA2576804C
CA2576804C CA2576804A CA2576804A CA2576804C CA 2576804 C CA2576804 C CA 2576804C CA 2576804 A CA2576804 A CA 2576804A CA 2576804 A CA2576804 A CA 2576804A CA 2576804 C CA2576804 C CA 2576804C
Authority
CA
Canada
Prior art keywords
sample
modification
labels
processing means
analysis
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2576804A
Other languages
English (en)
French (fr)
Other versions
CA2576804A1 (en
Inventor
Colin Blackmore
Patrick Courtney
Alistair Fitch
Shab Ladha
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PerkinElmer Singapore Pte Ltd
Original Assignee
PerkinElmer Singapore Pte Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0419325.6A external-priority patent/GB0419325D0/en
Application filed by PerkinElmer Singapore Pte Ltd filed Critical PerkinElmer Singapore Pte Ltd
Publication of CA2576804A1 publication Critical patent/CA2576804A1/en
Application granted granted Critical
Publication of CA2576804C publication Critical patent/CA2576804C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Optics & Photonics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CA2576804A 2004-09-01 2005-08-17 A method of analysing a sample and apparatus therefor Expired - Lifetime CA2576804C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB0419325.6 2004-09-01
GBGB0419325.6A GB0419325D0 (en) 2004-09-01 2004-09-01 A method of analysing a sample including fluorescent labels and apparatus therefor
GB0512811A GB2418018B (en) 2004-09-01 2005-06-23 A method of analysing a sample including fluorescent labels and apparatus therefor
GB0512811.1 2005-06-23
PCT/GB2005/003226 WO2006024819A2 (en) 2004-09-01 2005-08-17 A method of analysing a sample and apparatus therefor

Publications (2)

Publication Number Publication Date
CA2576804A1 CA2576804A1 (en) 2006-03-09
CA2576804C true CA2576804C (en) 2013-10-15

Family

ID=35079173

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2576804A Expired - Lifetime CA2576804C (en) 2004-09-01 2005-08-17 A method of analysing a sample and apparatus therefor

Country Status (6)

Country Link
EP (1) EP1784633B1 (https=)
JP (1) JP5318413B2 (https=)
KR (1) KR101286216B1 (https=)
AU (1) AU2005279041B2 (https=)
CA (1) CA2576804C (https=)
WO (1) WO2006024819A2 (https=)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4855139B2 (ja) 2006-05-18 2012-01-18 オリンパス株式会社 顕微鏡装置および細胞観察方法
US8664589B2 (en) * 2011-12-29 2014-03-04 Electro Scientific Industries, Inc Spectroscopy data display systems and methods
WO2014168734A1 (en) * 2013-03-15 2014-10-16 Cedars-Sinai Medical Center Time-resolved laser-induced fluorescence spectroscopy systems and uses thereof
WO2017173315A1 (en) 2016-04-01 2017-10-05 Black Light Surgical, Inc. Systems, devices, and methods for time-resolved fluorescent spectroscopy
DE102016109303A1 (de) * 2016-05-20 2017-11-23 Friedrich-Schiller-Universität Jena Lasermikroskop mit Ablationsfunktion
JP6033980B1 (ja) * 2016-06-01 2016-11-30 株式会社片岡製作所 細胞処理システム
JP6541160B2 (ja) * 2017-02-13 2019-07-10 株式会社片岡製作所 細胞処理装置および対象物の処理方法
JP6532063B2 (ja) 2017-02-13 2019-06-19 株式会社片岡製作所 細胞処理装置
JP6574028B1 (ja) 2018-06-29 2019-09-11 株式会社片岡製作所 細胞処理装置および細胞のレーザ処理方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4629687A (en) * 1982-07-29 1986-12-16 Board Of Trustees Of Michigan State University Positive selection sorting of cells
US5932872A (en) * 1994-07-01 1999-08-03 Jeffrey H. Price Autofocus system for scanning microscopy having a volume image formation
FI101829B1 (fi) * 1995-03-07 1998-08-31 Erkki Juhani Soini Biospesifinen määritysmenetelmä
FI98765C (fi) * 1995-01-16 1997-08-11 Erkki Soini Virtaussytometrinen menetelmä ja laite
EP0977981B1 (en) * 1997-04-25 2003-07-09 Universiteit van Amsterdam Photobleachable luminescent layers for calibration and standardization in optical microscopy
JP3735190B2 (ja) * 1997-10-28 2006-01-18 オリンパス株式会社 走査型サイトメータ
JPH11271220A (ja) * 1998-03-24 1999-10-05 Olympus Optical Co Ltd 走査型顕微鏡の測定パラメータ決定方法
AU5223899A (en) * 1998-07-27 2000-02-21 Ljl Biosystems, Inc. Apparatus and methods for spectroscopic measurements
JP3678397B2 (ja) * 1998-12-15 2005-08-03 富士写真フイルム株式会社 撮影システム
US20030026762A1 (en) * 1999-05-05 2003-02-06 Malmros Mark K. Bio-spectral imaging system and methods for diagnosing cell disease state
WO2000071028A1 (en) * 1999-05-26 2000-11-30 Photogen, Inc. Improved methods and apparatus for multi-photon photo-activation and detection of molecular agents
DE60005138T2 (de) * 2000-04-06 2004-06-03 Europäisches Laboratorium für Molekularbiologie Rechnergesteuertes Mikroskop
EP1351048A4 (en) * 2000-12-14 2007-02-28 Olympus Corp FLUOROMETRIC ANALYZER AND FLUOROMETRIC ANALYSIS
JP3734769B2 (ja) * 2001-11-09 2006-01-11 アイダエンジニアリング株式会社 蛍光強度測定方法および装置
GB0224067D0 (en) * 2002-10-16 2002-11-27 Perkinelmer Uk Ltd Improvements in and relating to imaging
US7271886B2 (en) * 2002-11-18 2007-09-18 Panomics, Inc. Uncaging device

Also Published As

Publication number Publication date
KR20070050085A (ko) 2007-05-14
EP1784633A2 (en) 2007-05-16
AU2005279041B2 (en) 2011-04-07
WO2006024819A2 (en) 2006-03-09
CA2576804A1 (en) 2006-03-09
JP2008511824A (ja) 2008-04-17
WO2006024819A8 (en) 2006-09-08
EP1784633B1 (en) 2011-11-09
JP5318413B2 (ja) 2013-10-16
KR101286216B1 (ko) 2013-07-15
WO2006024819A3 (en) 2006-05-18
AU2005279041A1 (en) 2006-03-09

Similar Documents

Publication Publication Date Title
van Munster et al. Fluorescence lifetime imaging microscopy (FLIM)
Broussard et al. Fluorescence resonance energy transfer microscopy as demonstrated by measuring the activation of the serine/threonine kinase Akt
JP5307539B2 (ja) 生体試料撮像方法および生体試料撮像装置
US7782457B2 (en) Optical microscopy with phototransformable optical labels
US10288863B2 (en) Optogenetics microscope
US7671345B2 (en) Method of analysing a sample and apparatus therefor
CN101351735B (zh) 获取生物源样本的图像的装置和方法
CA2576804C (en) A method of analysing a sample and apparatus therefor
US20070160175A1 (en) Systems and methods for force-fluorescence microscopy
Helmchen Two-photon functional imaging of neuronal activity
Buranachai et al. Fluorescence lifetime imaging in living cells
Elgass et al. The fluorescence lifetime of BRI1-GFP as probe for the noninvasive determination of the membrane potential in living cells
Foo et al. Fluorescence correlation and cross-correlation spectroscopy using fluorescent proteins for measurements of biomolecular processes in living organisms
Bhupathi et al. Single Molecule Imaging Using State-of-the-Art Microscopy Techniques
French Overview of fluorescence imaging for biophotonics
Di Bona Advanced Fluorescence Correlation Spectroscopy for The Study of Nuclear Dynamics
Tiwari Foundations and Structural Applications of Fluorescence Optical Imaging in Biological Systems
Palikaras Foundation for Research and Technology-Hellas, Heraklion, Crete, Greece Nektarios Tavernarakis, Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, Crete, Greece and Medical School, University of Crete, Heraklion, Crete, Greece
Denham Cellular signalling in sight: Technical challenges in microscopy
Merriman III Stimulated emission depletion (STED) microscopy and Pacific Orange dye optimization for H9c2 COX-1 imaging via indirect immunocytochemistry
Herman et al. FRET imaging microscopy
Helmchen 2 Two-Photon

Legal Events

Date Code Title Description
EEER Examination request