CA2569821A1 - Process for protein isolation - Google Patents
Process for protein isolation Download PDFInfo
- Publication number
- CA2569821A1 CA2569821A1 CA002569821A CA2569821A CA2569821A1 CA 2569821 A1 CA2569821 A1 CA 2569821A1 CA 002569821 A CA002569821 A CA 002569821A CA 2569821 A CA2569821 A CA 2569821A CA 2569821 A1 CA2569821 A1 CA 2569821A1
- Authority
- CA
- Canada
- Prior art keywords
- solution
- column
- separation medium
- plasma
- solid separation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 114
- 230000008569 process Effects 0.000 title claims abstract description 106
- 238000000164 protein isolation Methods 0.000 title description 6
- 210000002381 plasma Anatomy 0.000 claims abstract description 186
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 163
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 163
- 238000010828 elution Methods 0.000 claims abstract description 131
- 238000000926 separation method Methods 0.000 claims abstract description 107
- 239000007787 solid Substances 0.000 claims abstract description 103
- 108010054218 Factor VIII Proteins 0.000 claims abstract description 66
- 102000001690 Factor VIII Human genes 0.000 claims abstract description 66
- 229960000301 factor viii Drugs 0.000 claims abstract description 66
- 102100022641 Coagulation factor IX Human genes 0.000 claims abstract description 60
- 108010076282 Factor IX Proteins 0.000 claims abstract description 60
- 229960004222 factor ix Drugs 0.000 claims abstract description 60
- 235000010633 broth Nutrition 0.000 claims abstract description 17
- 239000003446 ligand Substances 0.000 claims abstract description 12
- WYKHFQKONWMWQM-UHFFFAOYSA-N 2-sulfanylidene-1h-pyridine-3-carboxylic acid Chemical compound OC(=O)C1=CC=CN=C1S WYKHFQKONWMWQM-UHFFFAOYSA-N 0.000 claims abstract description 8
- 210000002966 serum Anatomy 0.000 claims abstract description 8
- 125000006850 spacer group Chemical group 0.000 claims abstract description 8
- 239000011159 matrix material Substances 0.000 claims abstract description 7
- 102000009027 Albumins Human genes 0.000 claims description 52
- 108010088751 Albumins Proteins 0.000 claims description 52
- 108010049003 Fibrinogen Proteins 0.000 claims description 45
- 102000008946 Fibrinogen Human genes 0.000 claims description 45
- 229940012952 fibrinogen Drugs 0.000 claims description 45
- 239000000137 peptide hydrolase inhibitor Substances 0.000 claims description 37
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- 108060003951 Immunoglobulin Proteins 0.000 claims description 36
- 102000018358 immunoglobulin Human genes 0.000 claims description 36
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- 229920005989 resin Polymers 0.000 claims description 23
- 102000004338 Transferrin Human genes 0.000 claims description 22
- 108090000901 Transferrin Proteins 0.000 claims description 22
- 239000012581 transferrin Substances 0.000 claims description 22
- 239000002253 acid Substances 0.000 claims description 16
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 11
- 239000011707 mineral Substances 0.000 claims description 11
- -1 alkyl carboxylic acid Chemical class 0.000 claims description 10
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- 229960005348 antithrombin iii Drugs 0.000 claims description 9
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims description 8
- 239000002753 trypsin inhibitor Substances 0.000 claims description 8
- 102000004506 Blood Proteins Human genes 0.000 claims description 7
- 108010017384 Blood Proteins Proteins 0.000 claims description 7
- 239000004019 antithrombin Substances 0.000 claims description 7
- 102000012404 Orosomucoid Human genes 0.000 claims description 6
- 108010061952 Orosomucoid Proteins 0.000 claims description 6
- 102000007584 Prealbumin Human genes 0.000 claims description 6
- 108010071690 Prealbumin Proteins 0.000 claims description 6
- 210000004369 blood Anatomy 0.000 claims description 6
- 239000008280 blood Substances 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 6
- 239000003599 detergent Substances 0.000 claims description 6
- 125000003118 aryl group Chemical group 0.000 claims description 5
- 125000003700 epoxy group Chemical group 0.000 claims description 5
- 102000009091 Amyloidogenic Proteins Human genes 0.000 claims description 4
- 108010048112 Amyloidogenic Proteins Proteins 0.000 claims description 4
- 102000007592 Apolipoproteins Human genes 0.000 claims description 4
- 108010071619 Apolipoproteins Proteins 0.000 claims description 4
- 102100030802 Beta-2-glycoprotein 1 Human genes 0.000 claims description 4
- 102000016918 Complement C3 Human genes 0.000 claims description 4
- 108010028780 Complement C3 Proteins 0.000 claims description 4
- 102000004127 Cytokines Human genes 0.000 claims description 4
- 108090000695 Cytokines Proteins 0.000 claims description 4
- 102000008857 Ferritin Human genes 0.000 claims description 4
- 108050000784 Ferritin Proteins 0.000 claims description 4
- 238000008416 Ferritin Methods 0.000 claims description 4
- 108010088842 Fibrinolysin Proteins 0.000 claims description 4
- 108010067306 Fibronectins Proteins 0.000 claims description 4
- 102000015779 HDL Lipoproteins Human genes 0.000 claims description 4
- 108010010234 HDL Lipoproteins Proteins 0.000 claims description 4
- 108090001061 Insulin Proteins 0.000 claims description 4
- 102000013566 Plasminogen Human genes 0.000 claims description 4
- 108010051456 Plasminogen Proteins 0.000 claims description 4
- 108010001014 Plasminogen Activators Proteins 0.000 claims description 4
- 102000001938 Plasminogen Activators Human genes 0.000 claims description 4
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 claims description 4
- 108010023197 Streptokinase Proteins 0.000 claims description 4
- 229940122618 Trypsin inhibitor Drugs 0.000 claims description 4
- 108090000435 Urokinase-type plasminogen activator Proteins 0.000 claims description 4
- 102000003990 Urokinase-type plasminogen activator Human genes 0.000 claims description 4
- 102000050760 Vitamin D-binding protein Human genes 0.000 claims description 4
- 101710179590 Vitamin D-binding protein Proteins 0.000 claims description 4
- GKXVJHDEWHKBFH-UHFFFAOYSA-N [2-(aminomethyl)phenyl]methanamine Chemical compound NCC1=CC=CC=C1CN GKXVJHDEWHKBFH-UHFFFAOYSA-N 0.000 claims description 4
- 230000001772 anti-angiogenic effect Effects 0.000 claims description 4
- 230000010100 anticoagulation Effects 0.000 claims description 4
- 108010023562 beta 2-Glycoprotein I Proteins 0.000 claims description 4
- 229940009550 c1 esterase inhibitor Drugs 0.000 claims description 4
- 239000003527 fibrinolytic agent Substances 0.000 claims description 4
- 239000003102 growth factor Substances 0.000 claims description 4
- 229940125396 insulin Drugs 0.000 claims description 4
- 229940012957 plasmin Drugs 0.000 claims description 4
- 229940127126 plasminogen activator Drugs 0.000 claims description 4
- 239000002797 plasminogen activator inhibitor Substances 0.000 claims description 4
- 230000008742 procoagulation Effects 0.000 claims description 4
- 229960005202 streptokinase Drugs 0.000 claims description 4
- 229960000103 thrombolytic agent Drugs 0.000 claims description 4
- 229960005356 urokinase Drugs 0.000 claims description 4
- 230000002209 hydrophobic effect Effects 0.000 claims description 3
- 125000001072 heteroaryl group Chemical group 0.000 claims description 2
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 claims 1
- 102000016359 Fibronectins Human genes 0.000 claims 1
- 102100023915 Insulin Human genes 0.000 claims 1
- 239000000243 solution Substances 0.000 description 165
- 239000001509 sodium citrate Substances 0.000 description 62
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 62
- 235000002639 sodium chloride Nutrition 0.000 description 57
- 229940050528 albumin Drugs 0.000 description 48
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 46
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 42
- 239000012149 elution buffer Substances 0.000 description 41
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 33
- 239000011324 bead Substances 0.000 description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 26
- 239000011780 sodium chloride Substances 0.000 description 24
- 238000004587 chromatography analysis Methods 0.000 description 22
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 20
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 20
- 238000005406 washing Methods 0.000 description 20
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 19
- 238000011067 equilibration Methods 0.000 description 19
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 18
- 239000000872 buffer Substances 0.000 description 17
- 238000002955 isolation Methods 0.000 description 16
- 239000000126 substance Substances 0.000 description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N EtOH Substances CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 15
- 239000000047 product Substances 0.000 description 14
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 13
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 13
- 235000011130 ammonium sulphate Nutrition 0.000 description 13
- 239000007853 buffer solution Substances 0.000 description 13
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 13
- 229910052939 potassium sulfate Inorganic materials 0.000 description 13
- 235000011151 potassium sulphates Nutrition 0.000 description 13
- 229910052938 sodium sulfate Inorganic materials 0.000 description 13
- 235000011152 sodium sulphate Nutrition 0.000 description 13
- 238000011068 loading method Methods 0.000 description 12
- 150000007513 acids Chemical class 0.000 description 11
- 230000000694 effects Effects 0.000 description 11
- 150000002632 lipids Chemical class 0.000 description 11
- 229960005480 sodium caprylate Drugs 0.000 description 11
- BYKRNSHANADUFY-UHFFFAOYSA-M sodium octanoate Chemical compound [Na+].CCCCCCCC([O-])=O BYKRNSHANADUFY-UHFFFAOYSA-M 0.000 description 11
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 10
- 235000019270 ammonium chloride Nutrition 0.000 description 10
- 230000008827 biological function Effects 0.000 description 10
- 229910017604 nitric acid Inorganic materials 0.000 description 10
- 239000001103 potassium chloride Substances 0.000 description 10
- 235000011164 potassium chloride Nutrition 0.000 description 10
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 9
- BDAGIHXWWSANSR-UHFFFAOYSA-M Formate Chemical compound [O-]C=O BDAGIHXWWSANSR-UHFFFAOYSA-M 0.000 description 9
- 229910019142 PO4 Inorganic materials 0.000 description 9
- 235000010755 mineral Nutrition 0.000 description 9
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 9
- 239000010452 phosphate Substances 0.000 description 9
- 230000008929 regeneration Effects 0.000 description 9
- 238000011069 regeneration method Methods 0.000 description 9
- KDYFGRWQOYBRFD-UHFFFAOYSA-L succinate(2-) Chemical compound [O-]C(=O)CCC([O-])=O KDYFGRWQOYBRFD-UHFFFAOYSA-L 0.000 description 9
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- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 7
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- SEQKRHFRPICQDD-UHFFFAOYSA-N N-tris(hydroxymethyl)methylglycine Chemical compound OCC(CO)(CO)[NH2+]CC([O-])=O SEQKRHFRPICQDD-UHFFFAOYSA-N 0.000 description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 4
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- YAQXGBBDJYBXKL-UHFFFAOYSA-N iron(2+);1,10-phenanthroline;dicyanide Chemical compound [Fe+2].N#[C-].N#[C-].C1=CN=C2C3=NC=CC=C3C=CC2=C1.C1=CN=C2C3=NC=CC=C3C=CC2=C1 YAQXGBBDJYBXKL-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
- B01J20/28011—Other properties, e.g. density, crush strength
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/28—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
- B01J20/28002—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their physical properties
- B01J20/28004—Sorbent size or size distribution, e.g. particle size
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
- B01J20/289—Phases chemically bonded to a substrate, e.g. to silica or to polymers bonded via a spacer
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/30—Processes for preparing, regenerating, or reactivating
- B01J20/32—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating
- B01J20/3231—Impregnating or coating ; Solid sorbent compositions obtained from processes involving impregnating or coating characterised by the coating or impregnating layer
- B01J20/3242—Layers with a functional group, e.g. an affinity material, a ligand, a reactant or a complexing group
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/75—Fibrinogen
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
- B01D15/426—Specific type of solvent
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2004903045A AU2004903045A0 (en) | 2004-06-07 | Process for Protein Isolation | |
| AU2004903045 | 2004-06-07 | ||
| AU2004906128A AU2004906128A0 (en) | 2004-10-22 | Process for isolation of Factor VIII and/or Factor IX | |
| AU2004906128 | 2004-10-22 | ||
| PCT/AU2005/000796 WO2005121165A1 (en) | 2004-06-07 | 2005-06-06 | Process for protein isolation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2569821A1 true CA2569821A1 (en) | 2005-12-22 |
Family
ID=35503009
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002569821A Abandoned CA2569821A1 (en) | 2004-06-07 | 2005-06-06 | Process for protein isolation |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP1765849A4 (es) |
| JP (1) | JP2008511546A (es) |
| KR (1) | KR20070091098A (es) |
| BR (1) | BRPI0511893A (es) |
| CA (1) | CA2569821A1 (es) |
| IL (1) | IL179901A0 (es) |
| MX (1) | MXPA06014318A (es) |
| RU (1) | RU2007100351A (es) |
| WO (1) | WO2005121165A1 (es) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010515441A (ja) * | 2007-01-15 | 2010-05-13 | アップフロント・クロマトグラフィ・アクティーゼルスカブ | 原材料からのバイオ燃料およびタンパク質の製造 |
| CN101883631B (zh) * | 2007-12-03 | 2014-01-22 | 帝斯曼知识产权资产管理有限公司 | 用于生产珠粒的系统和方法 |
| DK2553095T3 (en) * | 2010-03-30 | 2017-01-23 | Octapharma Ag | PROCEDURE FOR CLEANING VITAMIN K-DEPENDENT PROTEINS SUCH AS COAGULATION FACTOR IX |
| EA030803B1 (ru) | 2014-01-29 | 2018-09-28 | Апфронт Кроматографи А/С | Способы разделения горохового белка |
| WO2020079108A1 (en) * | 2018-10-17 | 2020-04-23 | Csl Behring Gmbh | Process for purifying c1-inh |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE470099B (sv) * | 1984-05-17 | 1993-11-08 | Jerker Porath | Sulfonaktiverade tioeteradsorbenter för separation av t ex protein |
| ES2332893T3 (es) * | 1996-08-30 | 2010-02-15 | Upfront Chromatography A/S | Aislamiento de inmunoglobulinas. |
| WO2002096215A1 (en) * | 2001-06-01 | 2002-12-05 | Upfront Chromatography A/S | Fractionation of protein containing mixtures |
| US9624260B2 (en) * | 2004-06-07 | 2017-04-18 | Therapure Biopharma Inc. | Process for isolation of plasma or serum proteins |
-
2005
- 2005-06-06 KR KR1020077000516A patent/KR20070091098A/ko not_active Application Discontinuation
- 2005-06-06 MX MXPA06014318A patent/MXPA06014318A/es not_active Application Discontinuation
- 2005-06-06 EP EP05746991A patent/EP1765849A4/en not_active Withdrawn
- 2005-06-06 CA CA002569821A patent/CA2569821A1/en not_active Abandoned
- 2005-06-06 RU RU2007100351/13A patent/RU2007100351A/ru not_active Application Discontinuation
- 2005-06-06 JP JP2007526106A patent/JP2008511546A/ja active Pending
- 2005-06-06 WO PCT/AU2005/000796 patent/WO2005121165A1/en active Application Filing
- 2005-06-06 BR BRPI0511893-0A patent/BRPI0511893A/pt not_active Application Discontinuation
-
2006
- 2006-12-07 IL IL179901A patent/IL179901A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP1765849A4 (en) | 2007-09-05 |
| BRPI0511893A (pt) | 2008-01-15 |
| MXPA06014318A (es) | 2007-08-02 |
| RU2007100351A (ru) | 2008-07-20 |
| KR20070091098A (ko) | 2007-09-07 |
| EP1765849A1 (en) | 2007-03-28 |
| JP2008511546A (ja) | 2008-04-17 |
| WO2005121165A1 (en) | 2005-12-22 |
| IL179901A0 (en) | 2007-05-15 |
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