CA2569491A1 - 2'-nitrobenzyl-modified ribonucleotides - Google Patents
2'-nitrobenzyl-modified ribonucleotides Download PDFInfo
- Publication number
- CA2569491A1 CA2569491A1 CA002569491A CA2569491A CA2569491A1 CA 2569491 A1 CA2569491 A1 CA 2569491A1 CA 002569491 A CA002569491 A CA 002569491A CA 2569491 A CA2569491 A CA 2569491A CA 2569491 A1 CA2569491 A1 CA 2569491A1
- Authority
- CA
- Canada
- Prior art keywords
- ribonucleoside
- rna polymerase
- ribose
- ribonucleotide
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 125000002652 ribonucleotide group Chemical group 0.000 title claims abstract description 45
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 70
- 239000002773 nucleotide Substances 0.000 claims abstract description 53
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- 239000002336 ribonucleotide Substances 0.000 claims abstract description 37
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 27
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 25
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- 238000012163 sequencing technique Methods 0.000 claims abstract description 15
- 239000002342 ribonucleoside Substances 0.000 claims description 39
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- 230000002441 reversible effect Effects 0.000 claims description 35
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 claims description 28
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 claims description 28
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 claims description 27
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- -1 2--Nitrobenzyl group Chemical group 0.000 claims description 17
- 238000011282 treatment Methods 0.000 claims description 10
- ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 claims description 9
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 claims description 9
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- 230000002068 genetic effect Effects 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 description 1
- 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 239000012160 loading buffer Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 238000002703 mutagenesis Methods 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- 230000003094 perturbing effect Effects 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 150000003014 phosphoric acid esters Chemical class 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 125000000714 pyrimidinyl group Chemical group 0.000 description 1
- 238000002708 random mutagenesis Methods 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 229940063673 spermidine Drugs 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 239000003656 tris buffered saline Substances 0.000 description 1
- DRTQHJPVMGBUCF-UHFFFAOYSA-N uracil arabinoside Natural products OC1C(O)C(CO)OC1N1C(=O)NC(=O)C=C1 DRTQHJPVMGBUCF-UHFFFAOYSA-N 0.000 description 1
- 229940045145 uridine Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US57690704P | 2004-06-02 | 2004-06-02 | |
| US60/576,907 | 2004-06-02 | ||
| PCT/IB2005/002488 WO2005118608A2 (en) | 2004-06-02 | 2005-06-02 | 2’-nitrobenzyl-modified ribonucleotides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2569491A1 true CA2569491A1 (en) | 2005-12-15 |
Family
ID=35463420
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002569491A Abandoned CA2569491A1 (en) | 2004-06-02 | 2005-06-02 | 2'-nitrobenzyl-modified ribonucleotides |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US7759061B2 (enExample) |
| EP (1) | EP1753774B1 (enExample) |
| JP (3) | JP2008501679A (enExample) |
| AT (1) | ATE407939T1 (enExample) |
| AU (1) | AU2005250239A1 (enExample) |
| CA (1) | CA2569491A1 (enExample) |
| DE (1) | DE602005009693D1 (enExample) |
| WO (1) | WO2005118608A2 (enExample) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7947817B2 (en) | 2003-06-30 | 2011-05-24 | Roche Molecular Systems, Inc. | Synthesis and compositions of 2'-terminator nucleotides |
| US7572581B2 (en) | 2003-06-30 | 2009-08-11 | Roche Molecular Systems, Inc. | 2′-terminator nucleotide-related methods and systems |
| CA2569491A1 (en) | 2004-06-02 | 2005-12-15 | Asm Scientific, Inc. | 2'-nitrobenzyl-modified ribonucleotides |
| US7928207B2 (en) | 2004-06-28 | 2011-04-19 | Roche Molecular Systems, Inc | Synthesis and compositions of nucleic acids comprising 2′-terminator nucleotides |
| US7745125B2 (en) * | 2004-06-28 | 2010-06-29 | Roche Molecular Systems, Inc. | 2′-terminator related pyrophosphorolysis activated polymerization |
| EP1974057B1 (en) * | 2005-12-21 | 2012-06-13 | Roche Diagnostics GmbH | Sequencing and genotyping using reversibly 2'-modified nucleotides |
| US8399188B2 (en) * | 2006-09-28 | 2013-03-19 | Illumina, Inc. | Compositions and methods for nucleotide sequencing |
| US8486717B2 (en) | 2011-01-18 | 2013-07-16 | Symbolics, Llc | Lateral flow assays using two dimensional features |
| US9874556B2 (en) | 2012-07-18 | 2018-01-23 | Symbolics, Llc | Lateral flow assays using two dimensional features |
| US9146248B2 (en) | 2013-03-14 | 2015-09-29 | Intelligent Bio-Systems, Inc. | Apparatus and methods for purging flow cells in nucleic acid sequencing instruments |
| US9591268B2 (en) | 2013-03-15 | 2017-03-07 | Qiagen Waltham, Inc. | Flow cell alignment methods and systems |
| WO2015038978A1 (en) | 2013-09-13 | 2015-03-19 | Symbolics, Llc | Lateral flow assays using two dimensional test and control signal readout patterns |
| US10351918B2 (en) | 2013-10-30 | 2019-07-16 | Green Life Biotech, Llc | Pathogenesis quantification systems and treatment methods for citrus greening blight |
| JP2024518331A (ja) | 2021-04-29 | 2024-05-01 | アボット・ラボラトリーズ | 生体試料のハイスループット核酸検査 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58109498A (ja) * | 1981-08-24 | 1983-06-29 | Agency Of Ind Science & Technol | オリゴリボヌクレオチド |
| JPS5836396A (ja) * | 1981-08-24 | 1983-03-03 | Agency Of Ind Science & Technol | オリゴリボヌクレオチドの製造法 |
| US5223414A (en) | 1990-05-07 | 1993-06-29 | Sri International | Process for nucleic acid hybridization and amplification |
| CA2122203C (en) | 1993-05-11 | 2001-12-18 | Melinda S. Fraiser | Decontamination of nucleic acid amplification reactions |
| US20010018514A1 (en) * | 1998-07-31 | 2001-08-30 | Mcgall Glenn H. | Nucleic acid labeling compounds |
| US6136543A (en) * | 1997-01-31 | 2000-10-24 | Hitachi, Ltd. | Method for determining nucleic acids base sequence and apparatus therefor |
| EP1159453B1 (en) * | 1999-03-10 | 2008-05-28 | ASM Scientific, Inc. | A method for direct nucleic acid sequencing |
| JP2001288197A (ja) * | 2000-04-10 | 2001-10-16 | Fuji Photo Film Co Ltd | 蛍光ヌクレオチド |
| ATE356222T1 (de) * | 2000-10-06 | 2007-03-15 | Univ Columbia | Massives parallelverfahren zur dekodierung von dna und rna |
| CN1348096A (zh) * | 2000-10-10 | 2002-05-08 | 栾国彦 | 一种均相特异性检测核酸的探针及应用方法 |
| CA2425663C (en) * | 2000-10-11 | 2009-12-29 | Applera Corporation | Fluorescent nucleobase conjugates having anionic linkers |
| CA2476481C (en) | 2002-02-21 | 2016-01-26 | Asm Scientific, Inc. | Recombinase polymerase amplification |
| EP3985125A1 (en) | 2004-06-01 | 2022-04-20 | Abbott Diagnostics Scarborough, Inc. | Recombinase polymerase amplification |
| CA2569491A1 (en) * | 2004-06-02 | 2005-12-15 | Asm Scientific, Inc. | 2'-nitrobenzyl-modified ribonucleotides |
-
2005
- 2005-06-02 CA CA002569491A patent/CA2569491A1/en not_active Abandoned
- 2005-06-02 US US11/628,343 patent/US7759061B2/en active Active
- 2005-06-02 JP JP2007514217A patent/JP2008501679A/ja not_active Withdrawn
- 2005-06-02 AT AT05769767T patent/ATE407939T1/de not_active IP Right Cessation
- 2005-06-02 AU AU2005250239A patent/AU2005250239A1/en not_active Abandoned
- 2005-06-02 EP EP05769767A patent/EP1753774B1/en not_active Expired - Lifetime
- 2005-06-02 DE DE602005009693T patent/DE602005009693D1/de not_active Expired - Fee Related
- 2005-06-02 WO PCT/IB2005/002488 patent/WO2005118608A2/en not_active Ceased
-
2010
- 2010-06-21 US US12/803,261 patent/US8022194B2/en not_active Expired - Lifetime
-
2011
- 2011-08-10 US US13/206,926 patent/US8299226B2/en not_active Expired - Lifetime
-
2012
- 2012-01-25 JP JP2012013260A patent/JP2012095665A/ja active Pending
- 2012-05-25 JP JP2012119854A patent/JP2012157369A/ja active Pending
- 2012-10-23 US US13/658,023 patent/US20130045475A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2005118608A3 (en) | 2006-04-27 |
| ATE407939T1 (de) | 2008-09-15 |
| EP1753774B1 (en) | 2008-09-10 |
| US8299226B2 (en) | 2012-10-30 |
| DE602005009693D1 (de) | 2008-10-23 |
| US8022194B2 (en) | 2011-09-20 |
| AU2005250239A1 (en) | 2005-12-15 |
| JP2008501679A (ja) | 2008-01-24 |
| US20130045475A1 (en) | 2013-02-21 |
| US20110053154A1 (en) | 2011-03-03 |
| WO2005118608A2 (en) | 2005-12-15 |
| JP2012095665A (ja) | 2012-05-24 |
| US20120040345A1 (en) | 2012-02-16 |
| US7759061B2 (en) | 2010-07-20 |
| JP2012157369A (ja) | 2012-08-23 |
| US20080261208A1 (en) | 2008-10-23 |
| EP1753774A2 (en) | 2007-02-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |