CA2567624A1 - Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination - Google Patents
Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination Download PDFInfo
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- CA2567624A1 CA2567624A1 CA002567624A CA2567624A CA2567624A1 CA 2567624 A1 CA2567624 A1 CA 2567624A1 CA 002567624 A CA002567624 A CA 002567624A CA 2567624 A CA2567624 A CA 2567624A CA 2567624 A1 CA2567624 A1 CA 2567624A1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
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- Zoology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Biomedical Technology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US57455404P | 2004-05-25 | 2004-05-25 | |
| US60/574,554 | 2004-05-25 | ||
| US60204804P | 2004-08-16 | 2004-08-16 | |
| US60/602,048 | 2004-08-16 | ||
| PCT/US2005/018168 WO2005116237A2 (en) | 2004-05-25 | 2005-05-24 | Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination |
Publications (1)
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| CA2567624A1 true CA2567624A1 (en) | 2005-12-08 |
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|---|---|---|---|
| CA002567624A Abandoned CA2567624A1 (en) | 2004-05-25 | 2005-05-24 | Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for crystal structure determination |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US7399619B2 (enExample) |
| EP (1) | EP1774018A2 (enExample) |
| JP (1) | JP2008500050A (enExample) |
| AU (1) | AU2005248392A1 (enExample) |
| BR (1) | BRPI0511482A (enExample) |
| CA (1) | CA2567624A1 (enExample) |
| IL (1) | IL179211A0 (enExample) |
| MX (1) | MXPA06013586A (enExample) |
| WO (1) | WO2005116237A2 (enExample) |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| EP1490483B1 (en) * | 2001-04-19 | 2015-06-03 | The Scripps Research Institute | In vivo incorporation of unnatural amino acids |
| KR20050073559A (ko) * | 2002-10-16 | 2005-07-14 | 더 스크립스 리서치 인스티튜트 | 당단백질 합성 |
| CN103289960B (zh) | 2003-04-17 | 2017-04-26 | 斯克利普斯研究院 | 扩展真核生物遗传密码 |
| DE602004011789T2 (de) * | 2003-07-07 | 2009-02-12 | The Scripps Research Institute, La Jolla | Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen |
| JP2008513806A (ja) * | 2004-09-22 | 2008-05-01 | ザ スクリップス リサーチ インスティテュート | Ｎｍｒ試験のための蛋白質の部位特異的標識 |
| AU2005330514B2 (en) | 2004-10-27 | 2011-05-12 | The Scripps Research Institute | Orthogonal translation components for the in vivo incorporation of unnatural amino acids |
| CA2624290A1 (en) * | 2005-10-12 | 2007-04-26 | Scripps Research Institute | Selective posttranslational modification of phage-displayed polypeptides |
| WO2007092475A2 (en) | 2006-02-06 | 2007-08-16 | Franklin And Marshall College | Site-specific incorporation of fluorinated amino acids into proteins |
| CA2638763A1 (en) * | 2006-03-09 | 2007-09-13 | The Scripps Research Institute | System for the expression of orthogonal translation components in eubacterial host cells |
| RU2467069C2 (ru) * | 2006-03-09 | 2012-11-20 | Зе Скрипс Ресеч Инститьют | Система экспрессии компонентов ортогональной трансляции в эубактериальной клетке-хозяине |
| JP5249194B2 (ja) | 2006-03-16 | 2013-07-31 | ザ スクリプス リサーチ インスティチュート | 非天然アミノ酸フェニルセレノシステインを含有するタンパク質の遺伝的にプログラムされた発現 |
| BRPI0711811A2 (pt) * | 2006-05-23 | 2011-12-06 | Scripps Research Inst | aminoácidos de cumarina fluorescente geneticamente codificados |
| CA2663052A1 (en) * | 2006-09-21 | 2008-03-27 | The Scripps Research Institute | Genetically programmed expression of selectively sulfated proteins in eubacteria |
| US20080166766A1 (en) | 2006-10-18 | 2008-07-10 | The Scripps Research Institute | Genetic incorporation of unnatural amino acids into proteins in mammalian cells |
| KR20100088618A (ko) * | 2007-10-25 | 2010-08-09 | 더 스크립스 리서치 인스티튜트 | 리덕타제 내로의 3-아미노티로신의 유전적 도입 |
| BRPI0819092A2 (pt) | 2007-11-02 | 2014-10-14 | Scripps Research Inst | Evolução direcionada usando proteínas que compreendem aminoácidos não naturais. |
| US20090148887A1 (en) * | 2007-11-02 | 2009-06-11 | The Scripps Research Institute | Genetically encoded boronate amino acid |
| CN105647824A (zh) * | 2007-12-11 | 2016-06-08 | 斯克利普斯研究院 | 甲基营养酵母菌巴斯德毕赤酵母中的体内非天然氨基酸表达 |
| WO2009088949A1 (en) | 2008-01-03 | 2009-07-16 | Verenium Corporation | Transferases and oxidoreductases, nucleic acids encoding them and methods for making and using them |
| KR20100125290A (ko) * | 2008-02-08 | 2010-11-30 | 더 스크립스 리서치 인스티튜트 | 유전적으로 코딩된 비천연 아미노산에 의한 면역 내성의 파괴 |
| US20090197339A1 (en) * | 2008-12-10 | 2009-08-06 | The Scripps Research Institute | In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris |
| US8637306B2 (en) * | 2008-12-10 | 2014-01-28 | The Scripps Research Institute | Production of carrier-peptide conjugates using chemically reactive unnatural amino acids |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1490483B1 (en) | 2001-04-19 | 2015-06-03 | The Scripps Research Institute | In vivo incorporation of unnatural amino acids |
| DK1578949T3 (da) | 2002-10-16 | 2009-07-27 | Scripps Research Inst | Stedspecifik optagelse af keto-aminnosyrer hos proteiner |
| KR20050073559A (ko) | 2002-10-16 | 2005-07-14 | 더 스크립스 리서치 인스티튜트 | 당단백질 합성 |
| EP1583816A4 (en) | 2002-12-22 | 2007-06-13 | Scripps Research Inst | PROTEIN ARRAYS |
| CN103289960B (zh) | 2003-04-17 | 2017-04-26 | 斯克利普斯研究院 | 扩展真核生物遗传密码 |
| WO2005003294A2 (en) | 2003-06-18 | 2005-01-13 | The Scripps Research Institute | Unnatural reactive amino acid genetic code additions |
| WO2005007870A2 (en) | 2003-07-07 | 2005-01-27 | The Scripps Research Institute | COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF |
| DE602004011789T2 (de) | 2003-07-07 | 2009-02-12 | The Scripps Research Institute, La Jolla | Zusammensetzungen der orthogonalen Lysyl-tRNA und Aminoacyl-tRNA Synthetase Paaren und ihre Verwendungen |
| WO2005007624A2 (en) | 2003-07-07 | 2005-01-27 | The Scripps Research Institute | Compositions of orthogonal glutamyl-trna and aminoacyl trna synthetase pairs and uses thereof |
| WO2005038002A2 (en) | 2003-10-14 | 2005-04-28 | The Scripps Research Institute | Site-specific incorporation of redox active amino acids into proteins |
| BRPI0417801A (pt) | 2003-12-18 | 2007-04-10 | Scripps Research Inst | incorporação seletiva de 5-hidroxitriptofano em proteìnas de células mamìferas |
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- 2005-05-24 AU AU2005248392A patent/AU2005248392A1/en not_active Abandoned
- 2005-05-24 BR BRPI0511482-9A patent/BRPI0511482A/pt not_active IP Right Cessation
- 2005-05-24 US US11/137,850 patent/US7399619B2/en not_active Expired - Fee Related
- 2005-05-24 CA CA002567624A patent/CA2567624A1/en not_active Abandoned
- 2005-05-24 WO PCT/US2005/018168 patent/WO2005116237A2/en not_active Ceased
- 2005-05-24 MX MXPA06013586A patent/MXPA06013586A/es unknown
- 2005-05-24 JP JP2007515256A patent/JP2008500050A/ja active Pending
- 2005-05-24 EP EP05780072A patent/EP1774018A2/en not_active Withdrawn
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Also Published As
| Publication number | Publication date |
|---|---|
| IL179211A0 (en) | 2007-03-08 |
| WO2005116237A3 (en) | 2007-04-19 |
| MXPA06013586A (es) | 2007-11-09 |
| EP1774018A2 (en) | 2007-04-18 |
| US20050272121A1 (en) | 2005-12-08 |
| AU2005248392A1 (en) | 2005-12-08 |
| JP2008500050A (ja) | 2008-01-10 |
| US7399619B2 (en) | 2008-07-15 |
| WO2005116237A2 (en) | 2005-12-08 |
| BRPI0511482A (pt) | 2007-12-26 |
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