CA2546157C - Improved expression systems with sec-system secretion - Google Patents

Improved expression systems with sec-system secretion Download PDF

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Publication number
CA2546157C
CA2546157C CA2546157A CA2546157A CA2546157C CA 2546157 C CA2546157 C CA 2546157C CA 2546157 A CA2546157 A CA 2546157A CA 2546157 A CA2546157 A CA 2546157A CA 2546157 C CA2546157 C CA 2546157C
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CA
Canada
Prior art keywords
peptide
sequence
protein
recombinant protein
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2546157A
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English (en)
French (fr)
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CA2546157A1 (en
Inventor
Diane M. Retallack
Jane C. Schneider
Thomas Martin Ramseier
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pelican Technology Holdings Inc
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Pfenex Inc
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Publication of CA2546157A1 publication Critical patent/CA2546157A1/en
Application granted granted Critical
Publication of CA2546157C publication Critical patent/CA2546157C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/16—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
CA2546157A 2003-11-21 2004-11-22 Improved expression systems with sec-system secretion Expired - Lifetime CA2546157C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US52412403P 2003-11-21 2003-11-21
US60/524,124 2003-11-21
PCT/US2004/039316 WO2005089093A2 (en) 2003-11-21 2004-11-22 Improved expression systems with sec-system secretion

Publications (2)

Publication Number Publication Date
CA2546157A1 CA2546157A1 (en) 2005-09-29
CA2546157C true CA2546157C (en) 2014-07-22

Family

ID=34994140

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2546157A Expired - Lifetime CA2546157C (en) 2003-11-21 2004-11-22 Improved expression systems with sec-system secretion

Country Status (11)

Country Link
US (1) US7985564B2 (https=)
EP (4) EP1687324B1 (https=)
JP (1) JP5028551B2 (https=)
KR (1) KR101265343B1 (https=)
CN (1) CN1882605B (https=)
AU (1) AU2004317306B2 (https=)
BR (1) BRPI0416807A (https=)
CA (1) CA2546157C (https=)
MX (1) MXPA06005705A (https=)
PL (2) PL2336153T3 (https=)
WO (1) WO2005089093A2 (https=)

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US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
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EP2542574B1 (en) * 2010-03-04 2017-08-09 Pfenex Inc. Method for producing soluble recombinant interferon protein without denaturing
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CN103254277B (zh) * 2013-05-03 2017-02-08 南京工业大学 强分泌性信号肽增强小肽模序及其应用
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JP2017521055A (ja) 2014-06-20 2017-08-03 ダウ アグロサイエンシィズ エルエルシー 害虫防除に有用な植物性殺虫タンパク質
CN107532190B (zh) 2014-12-01 2021-07-09 菲尼克斯公司 用于肽生产的融合伴侣
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JP7339160B2 (ja) 2017-04-27 2023-09-05 ジュノ セラピューティクス ゲーエムベーハー オリゴマー粒子試薬およびその使用方法
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CA3077393A1 (en) 2017-10-27 2019-05-02 Pfenex Inc. Bacterial leader sequences for periplasmic protein expression
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CN113667685B (zh) * 2018-08-07 2023-02-28 康码（上海）生物科技有限公司 信号肽相关序列及其在蛋白质合成中的应用
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Also Published As

Publication number Publication date
KR101265343B1 (ko) 2013-05-20
EP2327718B1 (en) 2016-03-23
WO2005089093A3 (en) 2006-03-02
EP2336153B1 (en) 2016-03-30
AU2004317306A1 (en) 2005-09-29
EP1687324B1 (en) 2012-08-22
JP5028551B2 (ja) 2012-09-19
CA2546157A1 (en) 2005-09-29
PL2327718T3 (pl) 2016-10-31
EP1687324A2 (en) 2006-08-09
CN1882605A (zh) 2006-12-20
BRPI0416807A (pt) 2007-01-09
CN1882605B (zh) 2012-07-11
EP1687324A4 (en) 2007-08-22
AU2004317306B2 (en) 2010-09-02
JP2007512028A (ja) 2007-05-17
EP2327718A1 (en) 2011-06-01
US7985564B2 (en) 2011-07-26
WO2005089093A2 (en) 2005-09-29
KR20060123173A (ko) 2006-12-01
EP2336153A1 (en) 2011-06-22
EP2314602A1 (en) 2011-04-27
MXPA06005705A (es) 2006-08-23
PL2336153T3 (pl) 2016-08-31
US20060008877A1 (en) 2006-01-12

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