CA2488575A1 - Three dimensional vaginal tissue model containing immune cells - Google Patents

Three dimensional vaginal tissue model containing immune cells Download PDF

Info

Publication number
CA2488575A1
CA2488575A1 CA002488575A CA2488575A CA2488575A1 CA 2488575 A1 CA2488575 A1 CA 2488575A1 CA 002488575 A CA002488575 A CA 002488575A CA 2488575 A CA2488575 A CA 2488575A CA 2488575 A1 CA2488575 A1 CA 2488575A1
Authority
CA
Canada
Prior art keywords
cells
tissue
vaginal
cervico
medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002488575A
Other languages
English (en)
French (fr)
Inventor
Mitchell Klausner
Seyoum Ayehunie
Joseph Kubilus
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
MatTek Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2488575A1 publication Critical patent/CA2488575A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
    • C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/1114—T cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/11—Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/1121—Dendritic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/24—Genital tract cells, non-germinal cells from gonads
    • C12N2502/243—Cells of the female genital tract, non-germinal ovarian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/04—Screening or testing on artificial tissues

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CA002488575A 2002-06-07 2003-05-21 Three dimensional vaginal tissue model containing immune cells Abandoned CA2488575A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/165,267 2002-06-07
US10/165,267 US6943021B2 (en) 2002-06-07 2002-06-07 Three dimensional vaginal tissue model containing immune cells
PCT/US2003/016167 WO2003104393A2 (en) 2002-06-07 2003-05-21 Three dimensional vaginal tissue model containing immune cells

Publications (1)

Publication Number Publication Date
CA2488575A1 true CA2488575A1 (en) 2003-12-18

Family

ID=29710402

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002488575A Abandoned CA2488575A1 (en) 2002-06-07 2003-05-21 Three dimensional vaginal tissue model containing immune cells

Country Status (6)

Country Link
US (2) US6943021B2 (https=)
EP (1) EP1531677A4 (https=)
JP (1) JP4531558B2 (https=)
AU (1) AU2003239561A1 (https=)
CA (1) CA2488575A1 (https=)
WO (1) WO2003104393A2 (https=)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005014149A1 (ja) * 2003-08-07 2005-02-17 Asahi Kasei Kabushiki Kaisha 複合多孔膜とその製造方法
EP1937798A4 (en) * 2005-04-15 2009-05-13 Univ Columbia CHEMICALLY DEFINED CULTURAL MEDIA FOR THE EXPANSION AND DIFFERENTIATION OF EPIDERMIC CELLS AND USES THEREOF FOR THE IN VITRO FARMING OF HAIRFOLLICLES
JP4792566B2 (ja) * 2005-06-01 2011-10-12 財団法人ヒューマンサイエンス振興財団 樹状細胞を含む三次元培養ヒト皮膚モデル
EP1762609B1 (en) * 2005-09-13 2012-04-04 Takara Bio Inc. Serum-free medium for the production of retroviral vectors
WO2007031273A2 (en) * 2005-09-16 2007-03-22 Universität Bern Artificial immune-reconstituted epidermis equivalents
EP2260097B1 (en) * 2008-03-12 2017-04-26 Sanofi Pasteur VaxDesign Corporation Disease model incorporation into an artificial immune system (ais)
JP2010046058A (ja) * 2008-07-24 2010-03-04 Two Cells Co Ltd 治療用培養細胞の製造方法
CN103797110B (zh) 2011-07-12 2017-08-25 食品安全测试系统公司 沙门氏菌和大肠杆菌的培养基和培养方法以及用于检测沙门氏菌和大肠杆菌的方法
US9301920B2 (en) 2012-06-18 2016-04-05 Therapeuticsmd, Inc. Natural combination hormone replacement formulations and therapies
US8633178B2 (en) 2011-11-23 2014-01-21 Therapeuticsmd, Inc. Natural combination hormone replacement formulations and therapies
ITMI20120104A1 (it) * 2012-01-30 2013-07-31 Cutech S R L L'utilizzo di tessuto umano proveniente da piccole labbra dei genitali femminili
JP5977580B2 (ja) * 2012-05-24 2016-08-24 秀実 高橋 ランゲルハンス細胞の調製方法
US10806740B2 (en) 2012-06-18 2020-10-20 Therapeuticsmd, Inc. Natural combination hormone replacement formulations and therapies
US10806697B2 (en) 2012-12-21 2020-10-20 Therapeuticsmd, Inc. Vaginal inserted estradiol pharmaceutical compositions and methods
US20150196640A1 (en) 2012-06-18 2015-07-16 Therapeuticsmd, Inc. Progesterone formulations having a desirable pk profile
US20130338122A1 (en) 2012-06-18 2013-12-19 Therapeuticsmd, Inc. Transdermal hormone replacement therapies
US10568891B2 (en) 2012-12-21 2020-02-25 Therapeuticsmd, Inc. Vaginal inserted estradiol pharmaceutical compositions and methods
US11266661B2 (en) 2012-12-21 2022-03-08 Therapeuticsmd, Inc. Vaginal inserted estradiol pharmaceutical compositions and methods
US10537581B2 (en) 2012-12-21 2020-01-21 Therapeuticsmd, Inc. Vaginal inserted estradiol pharmaceutical compositions and methods
US10471072B2 (en) 2012-12-21 2019-11-12 Therapeuticsmd, Inc. Vaginal inserted estradiol pharmaceutical compositions and methods
US9180091B2 (en) 2012-12-21 2015-11-10 Therapeuticsmd, Inc. Soluble estradiol capsule for vaginal insertion
US11246875B2 (en) 2012-12-21 2022-02-15 Therapeuticsmd, Inc. Vaginal inserted estradiol pharmaceutical compositions and methods
EP3145489A1 (en) 2014-05-22 2017-03-29 TherapeuticsMD, Inc. Natural combination hormone replacement formulations and therapies
US10328087B2 (en) 2015-07-23 2019-06-25 Therapeuticsmd, Inc. Formulations for solubilizing hormones
GB2567269B (en) * 2015-10-22 2021-10-06 Univ Pennsylvania Systems and methods for producing micro-engineered models of the human cervix
CN105483085B (zh) * 2016-02-16 2019-02-26 广州赛莱拉干细胞科技股份有限公司 一种dc细胞培养方法及培养基
WO2017173044A1 (en) 2016-04-01 2017-10-05 Therapeuticsmd Inc. Steroid hormone compositions in medium chain oils
WO2017173071A1 (en) 2016-04-01 2017-10-05 Therapeuticsmd, Inc. Steroid hormone pharmaceutical composition
US10297085B2 (en) * 2016-09-28 2019-05-21 Intel Corporation Augmented reality creations with interactive behavior and modality assignments
US20180100142A1 (en) * 2016-10-06 2018-04-12 Mattek Corporation Methods and Kits for Production of Tissue Equivalents from Cryopreserved Cells
CN108342322A (zh) * 2018-02-24 2018-07-31 宁夏医科大学总医院 建立原代人子宫内膜上皮细胞气液相培养模型的方法
US11633405B2 (en) 2020-02-07 2023-04-25 Therapeuticsmd, Inc. Steroid hormone pharmaceutical formulations
CN113293126B (zh) * 2021-04-01 2023-11-07 广东博溪生物科技有限公司 一种人阴道粘膜模型的体外构建方法
WO2023278286A1 (en) * 2021-06-28 2023-01-05 Mattek Corporation Intestinal and respiratory tissue models containing enterocytes, fibroblasts, immune cells, and endothelial cells
CN115181719B (zh) * 2022-07-13 2023-09-15 福建省海西细胞生物工程有限公司 一种用于培养组织工程表皮的无血清培养基
CN118853540B (zh) * 2024-09-27 2024-12-10 济南磐升生物技术有限公司 一种人阴道上皮模型的体外构建方法

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL79289A (en) 1985-07-05 1992-01-15 Whitehead Biomedical Inst Introduction and expression of foreign genetic material into keratinocytes using a recombinant retrovirus
US5863531A (en) 1986-04-18 1999-01-26 Advanced Tissue Sciences, Inc. In vitro preparation of tubular tissue structures by stromal cell culture on a three-dimensional framework
US5266480A (en) 1986-04-18 1993-11-30 Advanced Tissue Sciences, Inc. Three-dimensional skin culture system
US5160490A (en) 1986-04-18 1992-11-03 Marrow-Tech Incorporated Three-dimensional cell and tissue culture apparatus
US4963489A (en) 1987-04-14 1990-10-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
US5032508A (en) 1988-09-08 1991-07-16 Marrow-Tech, Inc. Three-dimensional cell and tissue culture system
US5902741A (en) 1986-04-18 1999-05-11 Advanced Tissue Sciences, Inc. Three-dimensional cartilage cultures
US5061620A (en) 1990-03-30 1991-10-29 Systemix, Inc. Human hematopoietic stem cell
US5478739A (en) 1992-10-23 1995-12-26 Advanced Tissue Sciences, Inc. Three-dimensional stromal cell and tissue culture system
US5627025A (en) 1994-08-12 1997-05-06 The Rockefeller University Method for the identification of compounds capable of abrogating human immunodeficiency virus (HIV) infection of dendritic cells and T-lymphocytes
US5830682A (en) 1994-09-09 1998-11-03 Zymogenetics Preparation of immortalized cells
FR2743817B1 (fr) * 1996-01-23 1998-03-13 Oreal Equivalent de peau comprenant des cellules de langerhans
US5766944A (en) 1996-12-31 1998-06-16 Ruiz; Margaret Eileen T cell differentiation of CD34+ stem cells in cultured thymic epithelial fragments
WO1998051785A1 (en) * 1997-05-14 1998-11-19 The General Hospital Corporation Co-cultivation of cells in a micropatterned configuration
US5968546A (en) 1997-05-16 1999-10-19 Baur; Marcus Keratinocyte culture from precursor cells
EP1538162B1 (en) * 1999-02-24 2012-04-11 North Carolina State University Compositions for altering mucus secretion
DK1264877T3 (da) * 2000-03-16 2013-10-28 Cellseed Inc Celledyrkningsbæremateriale, fremgangsmåde til samdyrkning af celler og samdyrket cellelag opnået derved

Also Published As

Publication number Publication date
JP2005530500A (ja) 2005-10-13
WO2003104393A3 (en) 2004-08-05
US20030228686A1 (en) 2003-12-11
US6943021B2 (en) 2005-09-13
US20080003677A1 (en) 2008-01-03
AU2003239561A1 (en) 2003-12-22
WO2003104393A2 (en) 2003-12-18
JP4531558B2 (ja) 2010-08-25
AU2003239561A8 (en) 2003-12-22
EP1531677A2 (en) 2005-05-25
EP1531677A4 (en) 2006-11-29

Similar Documents

Publication Publication Date Title
US6943021B2 (en) Three dimensional vaginal tissue model containing immune cells
RAPOPORT Dog thyroid cells in monolayer tissue culture: adenosine 3', 5'-cyclic monophosphate response to thyrotropic hormone
Abir et al. Morphological study of fully and partially isolated early human follicles
Ibbotson et al. Identification and characterization of osteoclast-like cells and their progenitors in cultures of feline marrow mononuclear cells.
Dongari-Bagtzoglou et al. Development of a highly reproducible three-dimensional organotypic model of the oral mucosa
Lee et al. In vitro differentiation of germ cells from nonobstructive azoospermic patients using three-dimensional culture in a collagen gel matrix
Moll et al. Characterization of epidermal wound healing in a human skin organ culture model: Acceleration by transplanted keratinocytes1
US20140335616A1 (en) Defined systems for epithelial cell culture and use thereof
EP2297299A1 (en) Methods for preparing human skin substitutes from human pluripotent stem cells
US20060228339A1 (en) Methods of preparing transplantable product for treatment of skin defects
Hybbinette et al. Enzymatic dissociation of keratinocytes from human skin biopsies for in vitro cell propagation
Kirk et al. Selective growth of normal adult human urothelial cells in serum-free medium
Richards et al. Growth of mouse mammary gland end buds cultured in a collagen gel matrix
Stoner et al. Identification and culture of human bronchial epithelial cells
JP2003516711A (ja) 正常ヒト皮膚組織由来の不死化した細胞系
Imcke et al. Growth of human hair follicle keratinocytes in vitro: Ultrastructural features of a new model
Dahm et al. Cultivation and characterisation of primary and subcultured equine keratinocytes
Fossum et al. Long-term culture of human urothelial cells–a qualitative analysis
Casslén et al. Human endometrial epithelial cells grown on collagen in serum-free medium. Estrogen responsiveness and morphology
Sato et al. Characterization of bovine pancreatic ductal cells isolated by a perfusion-digestion technique
Liu et al. Development of low-and high-serum culture conditions for use of human oral fibroblasts in toxicity testing of dental materials
Dong et al. Enrichment of epidermal stem cells by rapid adherence and analysis of the reciprocal interaction of epidermal stem cells with neighboring cells using an organotypic system
Figenschau et al. A simplified serum-free method for preparation and cultivation of human granulosa-luteal cells.
Hautier et al. Assessment of organ culture for the conservation of human skin allografts
Grossman et al. Effect of donor age on cultivation of human oral mucosal keratinocytes

Legal Events

Date Code Title Description
EEER Examination request
FZDE Dead

Effective date: 20130522