CA2481495C - Primer extension based method employing nucleotides labelled via cleavable linkers - Google Patents

Primer extension based method employing nucleotides labelled via cleavable linkers Download PDF

Info

Publication number
CA2481495C
CA2481495C CA2481495A CA2481495A CA2481495C CA 2481495 C CA2481495 C CA 2481495C CA 2481495 A CA2481495 A CA 2481495A CA 2481495 A CA2481495 A CA 2481495A CA 2481495 C CA2481495 C CA 2481495C
Authority
CA
Canada
Prior art keywords
nucleotide
mole
label
primer
labeled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2481495A
Other languages
English (en)
French (fr)
Other versions
CA2481495A1 (en
Inventor
Charlotta Olsson
Nigel Tooke
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Qiagen GmbH
Original Assignee
Qiagen GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE0201024A external-priority patent/SE0201024D0/xx
Application filed by Qiagen GmbH filed Critical Qiagen GmbH
Publication of CA2481495A1 publication Critical patent/CA2481495A1/en
Application granted granted Critical
Publication of CA2481495C publication Critical patent/CA2481495C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CA2481495A 2002-04-04 2003-04-04 Primer extension based method employing nucleotides labelled via cleavable linkers Expired - Lifetime CA2481495C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US36959902P 2002-04-04 2002-04-04
SE0201024A SE0201024D0 (sv) 2002-04-04 2002-04-04 New method
US60/369,599 2002-04-04
SE0201024-7 2002-04-04
PCT/SE2003/000547 WO2003085135A1 (en) 2002-04-04 2003-04-04 New method

Publications (2)

Publication Number Publication Date
CA2481495A1 CA2481495A1 (en) 2003-10-16
CA2481495C true CA2481495C (en) 2011-08-30

Family

ID=28793693

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2481495A Expired - Lifetime CA2481495C (en) 2002-04-04 2003-04-04 Primer extension based method employing nucleotides labelled via cleavable linkers

Country Status (8)

Country Link
US (1) US20050244827A1 (enExample)
EP (1) EP1495137B1 (enExample)
JP (1) JP4230364B2 (enExample)
AT (1) ATE304610T1 (enExample)
AU (1) AU2003214765B2 (enExample)
CA (1) CA2481495C (enExample)
DE (1) DE60301622T2 (enExample)
WO (1) WO2003085135A1 (enExample)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
EP1790736A3 (en) 2000-10-06 2007-08-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
GB0129012D0 (en) 2001-12-04 2002-01-23 Solexa Ltd Labelled nucleotides
JP2006509040A (ja) 2002-08-23 2006-03-16 ソレックサ リミテッド 修飾されたヌクレオチド
US11008359B2 (en) 2002-08-23 2021-05-18 Illumina Cambridge Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
EP1594980B2 (en) * 2003-01-29 2017-08-16 454 Life Sciences Corporation Bead emulsion nucleic acid amplification
WO2005060345A2 (en) * 2003-12-22 2005-07-07 Biotage Ab New method for sequencing-by-synthesis
GB0517097D0 (en) 2005-08-19 2005-09-28 Solexa Ltd Modified nucleosides and nucleotides and uses thereof
US8399188B2 (en) 2006-09-28 2013-03-19 Illumina, Inc. Compositions and methods for nucleotide sequencing
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
EP2207900B1 (en) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Design and synthesis of cleavable fluorescent nucleotides as reversible terminators for dna sequencing by synthesis
EP3431615A3 (en) 2007-10-19 2019-02-20 The Trustees of Columbia University in the City of New York Dna sequencing with non-fluorescent nucleotide reversible terminators cleavable label modified nucleotide terminators
JP5431352B2 (ja) 2007-11-28 2014-03-05 ビーエーエスエフ ソシエタス・ヨーロピア 液体安定剤混合物
CA2714630A1 (en) 2008-02-07 2009-08-13 Pacific Biosciences Of California, Inc. Cis reactive oxygen quenchers integrated into linkers
US20100255487A1 (en) 2009-03-27 2010-10-07 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
US9146248B2 (en) 2013-03-14 2015-09-29 Intelligent Bio-Systems, Inc. Apparatus and methods for purging flow cells in nucleic acid sequencing instruments
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
US9591268B2 (en) 2013-03-15 2017-03-07 Qiagen Waltham, Inc. Flow cell alignment methods and systems
BR112015022448B1 (pt) 2013-03-15 2020-12-08 Illumina Cambridge Limited molécula de nucleotídeo ou nucleosídeo modificado, métodos para preparar o crescimento de polinucleotídeo complementar a polinucleotídeo alvo de fita simples em reação de sequenciação e para determinar a sequência de polinucleotídeo alvo de fita simples e kit
JP7091325B2 (ja) * 2016-11-04 2022-06-27 キアゲン サイエンシーズ， エルエルシー チオール含有切断試薬および酸化的洗浄液
BR112020010436A2 (pt) * 2018-01-26 2020-11-24 F. Hoffmann-La Roche Ag oligonucleotídeo radiomarcado, processos para a preparação de um oligonucleotídeo radiomarcado, uso do oligonucleotídeo radiomarcado, método para determinar a biodistribuição e farmacocinética e oligonucleotídeo

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4910300A (en) * 1985-12-11 1990-03-20 Chiron Corporation Method for making nucleic acid probes
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US5783384A (en) * 1992-01-13 1998-07-21 President And Fellows Of Harvard College Selection of binding-molecules
US5516664A (en) * 1992-12-23 1996-05-14 Hyman; Edward D. Enzymatic synthesis of repeat regions of oligonucleotides
EP0870832A4 (en) * 1995-12-27 2001-08-22 Takara Shuzo Co NEW DNA POLYMERASE
US20020055100A1 (en) * 1997-04-01 2002-05-09 Kawashima Eric H. Method of nucleic acid sequencing
US6485944B1 (en) * 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
US6280947B1 (en) * 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
DE60044490D1 (de) * 1999-02-23 2010-07-15 Caliper Life Sciences Inc Manipulation von mikroteilchen in mikrofluiden systemen
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
AU2001265338A1 (en) * 2000-06-02 2001-12-17 Dna Sciences, Inc. Primer extension using a mixture of labelled and unlabelled nucleotides

Also Published As

Publication number Publication date
JP2005521422A (ja) 2005-07-21
DE60301622D1 (de) 2005-10-20
WO2003085135A1 (en) 2003-10-16
AU2003214765A1 (en) 2003-10-20
EP1495137A1 (en) 2005-01-12
US20050244827A1 (en) 2005-11-03
ATE304610T1 (de) 2005-09-15
JP4230364B2 (ja) 2009-02-25
CA2481495A1 (en) 2003-10-16
AU2003214765B2 (en) 2007-07-26
DE60301622T2 (de) 2006-06-14
EP1495137B1 (en) 2005-09-14

Similar Documents

Publication Publication Date Title
CA2481495C (en) Primer extension based method employing nucleotides labelled via cleavable linkers
JP4499987B2 (ja) 非−標準塩基を使用する固体支持体アッセイ系及び方法
AU770217B2 (en) Ligation assembly and detection of polynucleotides on solid-support
US7414115B2 (en) Length determination of nucleic acid repeat sequences by discontinuous primer extension
US20060024711A1 (en) Methods for nucleic acid amplification and sequence determination
AU2002213175A1 (en) Solid support assay systems and methods utilizing non-standard bases
EP1590482B1 (en) Nucleic acid amplification using non-standard bases
KR101757473B1 (ko) ｈＣＴＯ를 이용하는 ＰＴＯ 절단 및 연장 분석에 의한 고상에서의 타겟 핵산 서열 검출
AU4669100A (en) Nucleotide extension on a microarray of gel-immobilized primers
EP1631681A2 (en) Method for identifying characteristics of molecules by converting said characteristics into a polynucleotide sequence
CN100385013C (zh) 使用非标准碱基的固相支持体分析系统和方法

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20230404