CA2474695A1 - Bioluminescent protease assay - Google Patents

Bioluminescent protease assay Download PDF

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Publication number
CA2474695A1
CA2474695A1 CA002474695A CA2474695A CA2474695A1 CA 2474695 A1 CA2474695 A1 CA 2474695A1 CA 002474695 A CA002474695 A CA 002474695A CA 2474695 A CA2474695 A CA 2474695A CA 2474695 A1 CA2474695 A1 CA 2474695A1
Authority
CA
Canada
Prior art keywords
compound
substrate
aminoluciferin
peptide
carboxy
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002474695A
Other languages
English (en)
French (fr)
Inventor
Martha O'brien
Keith Wood
Dieter Klaubert
Bill Daily
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Promega Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2474695A1 publication Critical patent/CA2474695A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D277/00—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings
    • C07D277/60—Heterocyclic compounds containing 1,3-thiazole or hydrogenated 1,3-thiazole rings condensed with carbocyclic rings or ring systems
    • C07D277/62—Benzothiazoles
    • C07D277/64—Benzothiazoles with only hydrocarbon or substituted hydrocarbon radicals attached in position 2
    • C07D277/66—Benzothiazoles with only hydrocarbon or substituted hydrocarbon radicals attached in position 2 with aromatic rings or ring systems directly attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
CA002474695A 2002-02-01 2003-01-31 Bioluminescent protease assay Abandoned CA2474695A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US35315802P 2002-02-01 2002-02-01
US60/353,158 2002-02-01
PCT/US2003/002936 WO2003066611A1 (en) 2002-02-01 2003-01-31 Bioluminescent protease assay

Publications (1)

Publication Number Publication Date
CA2474695A1 true CA2474695A1 (en) 2003-08-14

Family

ID=27734277

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002474695A Abandoned CA2474695A1 (en) 2002-02-01 2003-01-31 Bioluminescent protease assay

Country Status (6)

Country Link
US (4) US7148030B2 (US20030211560A1-20031113-C00002.png)
EP (2) EP2388254B1 (US20030211560A1-20031113-C00002.png)
JP (1) JP4451663B2 (US20030211560A1-20031113-C00002.png)
AU (1) AU2003216139B2 (US20030211560A1-20031113-C00002.png)
CA (1) CA2474695A1 (US20030211560A1-20031113-C00002.png)
WO (1) WO2003066611A1 (US20030211560A1-20031113-C00002.png)

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US8227205B2 (en) 2007-04-13 2012-07-24 Promega Corporation Luminescent live and dead cell assay
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EP2269064B1 (en) * 2008-03-27 2016-11-30 Promega Corporation Protein labeling with cyanobenzothiazole conjugates
WO2010021686A1 (en) 2008-08-18 2010-02-25 Promega Corporation Luminogenic compounds and methods to detect cytochrome p450 3a enzymes
US20110165611A1 (en) * 2008-09-04 2011-07-07 Chun Li Dual modality detection of apoptosis
WO2010030343A1 (en) * 2008-09-09 2010-03-18 Promega Corporation Bioluminescent assays using cyanobenzothiazole compounds
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JP5905402B2 (ja) * 2010-03-11 2016-04-20 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ シアノベンゾチアゾール化合物を使用する生物発光アッセイ
US20120021923A1 (en) * 2010-05-04 2012-01-26 Contag Christopher H Methods and systems to diagnose a condition in an individual
WO2012061477A1 (en) * 2010-11-02 2012-05-10 Promega Corporation Coelenterazine derivatives and methods of using same
SG10202103336SA (en) 2010-11-02 2021-04-29 Promega Corp Novel coelenterazine substrates and methods of use
JP6067019B2 (ja) 2011-09-02 2017-01-25 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 代謝的に活性な細胞の酸化還元状態を評価するための化合物及び方法、ならびにｎａｄ（ｐ）／ｎａｄ（ｐ）ｈを測定するための方法
CN104458709A (zh) * 2013-09-12 2015-03-25 中国药科大学 一种筛选钙激活中性蛋白酶-1抑制剂高通量筛选方法
WO2015116867A1 (en) 2014-01-29 2015-08-06 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
JP6588917B2 (ja) 2014-01-29 2019-10-09 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ 生細胞への適用のためのプロ基質
CA3016107A1 (en) 2016-04-06 2017-10-12 Nestec S.A. Biomarkers for predicting degree of weight loss
JP2020150830A (ja) * 2019-03-19 2020-09-24 東亜ディーケーケー株式会社 唾液のプロテアーゼ活性の測定方法、及び口腔内細菌の活動量の評価方法

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Also Published As

Publication number Publication date
US20060121546A1 (en) 2006-06-08
US7666987B2 (en) 2010-02-23
AU2003216139B2 (en) 2008-08-14
WO2003066611A1 (en) 2003-08-14
US8071328B2 (en) 2011-12-06
US7384758B2 (en) 2008-06-10
EP1472238A1 (en) 2004-11-03
EP1472238B1 (en) 2012-07-04
EP2388254B1 (en) 2016-08-10
US20060183177A1 (en) 2006-08-17
US20100249427A1 (en) 2010-09-30
JP2005530485A (ja) 2005-10-13
US7148030B2 (en) 2006-12-12
JP4451663B2 (ja) 2010-04-14
AU2003216139A1 (en) 2003-09-02
US20030211560A1 (en) 2003-11-13
EP2388254A1 (en) 2011-11-23

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued