CA2464460A1 - Micro-organes in vitro, et utilisations correspondantes - Google Patents

Micro-organes in vitro, et utilisations correspondantes Download PDF

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Publication number
CA2464460A1
CA2464460A1 CA002464460A CA2464460A CA2464460A1 CA 2464460 A1 CA2464460 A1 CA 2464460A1 CA 002464460 A CA002464460 A CA 002464460A CA 2464460 A CA2464460 A CA 2464460A CA 2464460 A1 CA2464460 A1 CA 2464460A1
Authority
CA
Canada
Prior art keywords
organ
micro
cells
explant
recombinant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002464460A
Other languages
English (en)
Inventor
Eduardo N. Mitrani
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Yissum Research Development Co of Hebrew University of Jerusalem
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority claimed from PCT/IL2001/000976 external-priority patent/WO2003035851A1/fr
Publication of CA2464460A1 publication Critical patent/CA2464460A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

Abstract

L'invention se rapporte à des cultures de micro-organes comprenant des populations isolées de cellules possédant des caractéristiques spécifiques. Les cultures de micro-organes de l'invention présentent notamment la particularité de pouvoir être maintenues en culture sur de relativement longues périodes, ainsi que de préserver une microarchitecture d'organe qui facilite, par exemple, des interactions cellule-cellule et cellule-matrice analogues à celles survenant dans l'organe source. Les cultures de micro-organes de l'invention peuvent être utilisées dans des procédés destinés à fournir des produits génétiques à des sujets receveurs, à identifier des agents de prolifération cellulaire et de différenciation cellulaire, et à identifier et isoler des cellules souches. De plus, les cultures de micro-organes de la présente invention peuvent être utilisées dans des procédés d'identification d'inhibiteurs de la prolifération cellulaire, de la différenciation cellulaire et de l'infectiosité virale. Dans d'autres modes de réalisation, les cultures de micro-organes peuvent servir à la transplantation.
CA002464460A 2001-10-23 2001-10-23 Micro-organes in vitro, et utilisations correspondantes Abandoned CA2464460A1 (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IL2001/000976 WO2003035851A1 (fr) 2001-10-23 2001-10-23 Micro-organes in vitro, et utilisations correspondantes

Publications (1)

Publication Number Publication Date
CA2464460A1 true CA2464460A1 (fr) 2003-05-01

Family

ID=32676735

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002464460A Abandoned CA2464460A1 (fr) 2001-10-23 2001-10-23 Micro-organes in vitro, et utilisations correspondantes

Country Status (5)

Country Link
JP (1) JP2005506084A (fr)
KR (2) KR20040070343A (fr)
CN (1) CN1582330A (fr)
CA (1) CA2464460A1 (fr)
IL (1) IL161472A0 (fr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7468242B2 (en) 2001-11-05 2008-12-23 Medgenics, Inc. Dermal micro organs, methods and apparatuses for producing and using the same
US8501396B2 (en) 2001-11-05 2013-08-06 Medgenics Medical Israel Ltd. Dermal micro-organs, methods and apparatuses for producing and using the same
US8088568B2 (en) 2001-11-05 2012-01-03 Medgentics, Inc. Dermal micro-organs, methods and apparatuses for producing and using the same
EP2377403A1 (fr) * 2003-05-01 2011-10-19 Medgenics, Inc. Micro-organe dermique génétiquement modifié exprimant le facteur VIII
US8454948B2 (en) 2006-09-14 2013-06-04 Medgenics Medical Israel Ltd. Long lasting drug formulations
JP2010504909A (ja) 2006-09-14 2010-02-18 メドジェニクス・メディカル・イスラエル・リミテッド 長期持続性の医薬製剤
US8771930B2 (en) * 2007-05-18 2014-07-08 Lifeline Scientific, Inc. Ex vivo methods for testing toxicity of substances using donated human organs or tissues
CN103260649A (zh) * 2010-06-15 2013-08-21 迈德詹尼克斯医疗以色列有限公司 长效药物制剂
RU2631807C2 (ru) * 2011-06-02 2017-09-26 Президент Энд Феллоуз Ов Харвард Колледж Способы и применение систем культуры ткани ex vivo
WO2013096741A2 (fr) * 2011-12-23 2013-06-27 Anthrogenesis Corporation Organoïdes comprenant un échafaudage vasculaire placentaire repeuplé et décellularisé
CN105334196B (zh) * 2015-09-24 2018-08-24 华东师范大学 一种检测3D类器官中P-gp介导的Rh123转运的方法及应用
RU2631005C1 (ru) * 2016-10-06 2017-09-15 Федеральное государственное бюджетное образовательное учреждение высшего образования "Российский национальный исследовательский медицинский университет им. Н.И. Пирогова" Министерства здравоохранения Российской Федерации (ФГБОУ ВО РНИМУ им. Н.И. Пирогова Минздрава России) Способ культивирования клеток слюнной железы человека
CN109082402B (zh) * 2018-08-06 2021-08-17 复旦大学 一种aav-dj型腺相关病毒体外高效感染类器官的用途
CN113774018B (zh) * 2021-08-30 2023-09-05 吉林大学 一种大鼠心肌细胞和心肌成纤维细胞分离和培养的方法

Also Published As

Publication number Publication date
CN1582330A (zh) 2005-02-16
IL161472A0 (en) 2004-09-27
KR20040070343A (ko) 2004-08-07
KR20090060381A (ko) 2009-06-11
JP2005506084A (ja) 2005-03-03
KR100970195B1 (ko) 2010-07-14

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EEER Examination request
FZDE Discontinued

Effective date: 20140606