CA2449560A1 - Extension cyclique a basse temperature d'adn a haute specificite d'amorcage - Google Patents

Extension cyclique a basse temperature d'adn a haute specificite d'amorcage Download PDF

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Publication number
CA2449560A1
CA2449560A1 CA002449560A CA2449560A CA2449560A1 CA 2449560 A1 CA2449560 A1 CA 2449560A1 CA 002449560 A CA002449560 A CA 002449560A CA 2449560 A CA2449560 A CA 2449560A CA 2449560 A1 CA2449560 A1 CA 2449560A1
Authority
CA
Canada
Prior art keywords
dna
primer
bacillus
dna polymerase
cycle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002449560A
Other languages
English (en)
Inventor
Guo Fan Hong
Yongjie Yang
Jia Zhu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Mendel DNA Center Co Ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/878,131 external-priority patent/US20030087237A1/en
Application filed by Individual filed Critical Individual
Publication of CA2449560A1 publication Critical patent/CA2449560A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/113—Cycle sequencing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes d'extension d'une amorce ou d'une paire d'amorces dans l'amplification d'ADN cyclique à basse température de séquençage de cycle et PCR. Ces méthodes consistent en particulier à utiliser de façon combinée des ADN polymérases modérément thermostables en présence d'une faible concentration de glycérol ou d'éthylène glycol, ou de mélanges de ceux-ci, comme agents destinés à réduire la température de fusion de l'ADN (c'est-à-dire la température à laquelle les deux brins d'ADN sont dénaturés). L'invention concerne également des mélanges de réaction prédistribués d'ADN polymérase haute fidélité à grande capacité de traitement, stables à température ambiante pendant plusieurs semaines dans des kits prêts à l'emploi.
CA002449560A 2001-06-08 2002-06-05 Extension cyclique a basse temperature d'adn a haute specificite d'amorcage Abandoned CA2449560A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/878,131 US20030087237A1 (en) 2001-04-30 2001-06-08 Low-temperature cycle extension of DNA with high polymerization specificity
US09/878,131 2001-06-08
PCT/IB2002/003341 WO2002101004A2 (fr) 2001-06-08 2002-06-05 Extension cyclique a basse temperature d'adn a haute specificite d'amorcage

Publications (1)

Publication Number Publication Date
CA2449560A1 true CA2449560A1 (fr) 2002-12-19

Family

ID=25371448

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002449560A Abandoned CA2449560A1 (fr) 2001-06-08 2002-06-05 Extension cyclique a basse temperature d'adn a haute specificite d'amorcage

Country Status (5)

Country Link
EP (1) EP1436391A4 (fr)
JP (1) JP2005514003A (fr)
CA (1) CA2449560A1 (fr)
RU (1) RU2004100107A (fr)
WO (1) WO2002101004A2 (fr)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
ES2359322T3 (es) * 2004-10-18 2011-05-20 Brandeis University Procedimiento para la amplificación de ácidos nucleicos.
WO2007123742A2 (fr) * 2006-03-31 2007-11-01 Codon Devices, Inc. Méthodes et compositions améliorant la fidélité d'assemblage de plusieurs acides nucléiques
WO2008027558A2 (fr) 2006-08-31 2008-03-06 Codon Devices, Inc. Assemblage itératif d'acides nucléiques utilisant l'activation de caractères codés par vecteurs
JP2010246529A (ja) * 2009-03-27 2010-11-04 Toyobo Co Ltd 核酸増幅のための新規な核酸融解温度調整方法
WO2011056872A2 (fr) 2009-11-03 2011-05-12 Gen9, Inc. Procédés et dispositifs microfluidiques pour la manipulation de gouttelettes dans un ensemble polynucléotidique haute fidélité
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
WO2011085075A2 (fr) 2010-01-07 2011-07-14 Gen9, Inc. Assemblage de polynucléotides haute fidélité
EP2638157B1 (fr) 2010-11-12 2015-07-22 Gen9, Inc. Procédés et dispositifs pour la synthèse d'acides nucléiques
EP4039363A1 (fr) 2010-11-12 2022-08-10 Gen9, Inc. Puces à protéines et leurs procédés d'utilisation et de fabrication
LT2944693T (lt) 2011-08-26 2019-08-26 Gen9, Inc. Kompozicijos ir būdai, skirti nukleorūgščių didelio tikslumo sąrankai
US20130260422A1 (en) 2011-09-01 2013-10-03 New England Biolabs, Inc. Compositions and Methods Relating to Variant DNA Polymerases and Synthetic DNA Polymerases
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP4001427A1 (fr) 2012-04-24 2022-05-25 Gen9, Inc. Procédés de tri d'acides nucléiques et de clonage in vitro multiplex préparatoire
CN104685116A (zh) 2012-06-25 2015-06-03 Gen9股份有限公司 用于核酸组装和高通量测序的方法
JP6402905B2 (ja) * 2014-08-08 2018-10-10 東洋紡株式会社 核酸増幅の非特異反応を抑制する新規の方法
CN113981045A (zh) * 2021-11-23 2022-01-28 广州达安基因股份有限公司 制备恒温扩增混合酶系的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0614780A (ja) * 1992-06-30 1994-01-25 Takara Shuzo Co Ltd α型ＤＮＡポリメラーゼ遺伝子のクローニング方法
US5432065A (en) * 1993-03-30 1995-07-11 United States Biochemical Corporation Cycle sequencing with non-thermostable DNA polymerases
US6100078A (en) * 1994-04-01 2000-08-08 Gen-Probe Incorporated Purified DNA polymerase from bacillus stearothermophilus ATCC 12980
US5834253A (en) * 1994-11-17 1998-11-10 Shanghai Institute Of Biochemistry, Chinese Academy Of Sciences Bacillus stearothermophilus DNA polymerase with proof-reading 3'-5' exonuclease activity

Also Published As

Publication number Publication date
WO2002101004A3 (fr) 2004-04-29
EP1436391A4 (fr) 2005-02-16
JP2005514003A (ja) 2005-05-19
WO2002101004A2 (fr) 2002-12-19
RU2004100107A (ru) 2005-06-10
EP1436391A2 (fr) 2004-07-14

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Legal Events

Date Code Title Description
FZDE Discontinued