JPH05508302A - Ph129dnaポリメラーゼおよび該ポリメラーゼをコードするdna断片を用いるイン・ビトロdna合成反応 - Google Patents
Ph129dnaポリメラーゼおよび該ポリメラーゼをコードするdna断片を用いるイン・ビトロdna合成反応Info
- Publication number
- JPH05508302A JPH05508302A JP2508123A JP50812390A JPH05508302A JP H05508302 A JPH05508302 A JP H05508302A JP 2508123 A JP2508123 A JP 2508123A JP 50812390 A JP50812390 A JP 50812390A JP H05508302 A JPH05508302 A JP H05508302A
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- dna
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- dna polymerase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Claims (24)
- 1.DNA分子にハイブリダイズで奉るプライマー分子と共に該DNA分子をア ニールし; 4種の異なるデオキシヌクレオシド三リン酸、DNAポリメラーゼ、および特異 的ヌクレオチド塩基にてDNA合成を停止する1種またはそれ以上のDNA合成 停止剤を含有する容器中で該アニールした混合物をインキュベートし、ここに、 該停止剤は異なるヌクレオチド塩基でDNA合成を停止し;次いで、インキュベ ーティング反応のDNA生成物をサイズに従って分離し、それにより、該DNA のヌクレオチド塩基配列の少なくとも一部が決定できる工程よりなるDNA分子 のヌクレオチド塩基配列を測定する方法において、核DNAポリメラーゼがφ2 9タイプDNAポリメラーゼである改良。
- 2.該φ29−タイプDNAポリメラーゼが、φ29−タイプファージで感染し た細胞におけるそのポリメラーゼである請求項1記載の方法。
- 3.該φ29−タイプDNAポリメラーゼが、φ29、Cp−1、PRD1、φ 15、φ21、PZE、PZA、Nf、M2Y、B103、SF5、GA−1、 Cp−5、Cp−7、PR4、PR5、PR722、およびL17から選択され る請求項1記載の方法。
- 4.該φ29−タイプDNAポリメラーゼが、天然に存在するポリメラーゼのエ キソヌクレアーゼ活性の10%未満を有する修飾されたポリメラーゼである請求 項1記載の方法。
- 5.該ポリメラーゼが、天然に存在するエキソヌクレアーゼ活性の1%未満を有 するように修飾された請求項4記載の方法。
- 6.該φ29−タイプDNAポリメラーゼがエキソヌクレアーゼ活性を実質的に 有しない請求項5記載の方法。
- 7.該停止剤がジデオキシヌクレオシド三リン酸である請求項1記載の方法。
- 8.φ29−タイプDNAポリメラーゼ、および鎖停止剤よりなるDNA配列決 定用キット。
- 9.修飾されたφ29−タイプDNAポリメラーゼをコードするDNA断片であ って、該ポリメラーゼが、DNA配列決定で用いるのに十分なDNAポリメラー ゼ活性を有し、対応する天然に存在するφ29−タイプDNAポリメラーゼの活 性の10%未満であるエキソヌクレアーゼ活性を有する該DNA断片。
- 10.該DNA断片が実質的にエキソヌクレアーゼ活性を有しないDNAポリメ ラーゼをコードする請求項9記載の断片。
- 11.該DNA断片が、φ29DNAポリメラーゼをコードするDNA配列より なり、ここに該ポリメラーゼの12、14または66位のアミノ酸部位がアラニ ン部位によって置き換えられている請求の範囲第9項記載のDNA断片。
- 12.請求の範囲第9項または10項記載のDNA断片によって産生されたφ2 9−タイプDNAポリメラーゼ。
- 13.第1および第2プライマーを二本鎖DNA配列の対向鎖に対してアニール し、次いで、該アニールした混合物をDNAポリメラーゼと共にインキュベート することよりなるDNA配列の増幅方法において、該DNAポリメラーゼがφ2 9−タイプDNAポリメラーゼよりなることを特徴とする改良。
- 14.該第1および第2プライマーがアニーリングの後に相互に向けられるその 3′端を有する請求の範囲第13項記載の方法。
- 15.該方法が、さらに、該インキュベーション工程の後に、得られたDNAを 変性し、該第1および第2プライマーを該変性されたDNAに対してアニールし 、最後の該アニールされた混合物を該ポリメラーゼと共にインキュベートするこ とよりなる請求の範囲第13項記載の方法。
- 16.変性、アニーリング、およびインキニベーションの該サイクルを10〜4 0回繰り返す請求の範囲第15項記載の方法。
- 17.該φ29−タイプDNAポリメラーゼがφ29、Cp−1、PRD1、φ 15、φ21、PZE、PZA、Nf、M2Y、B103、SF5、GA−1、 Cp−5、Cp−7、PR4、PR5、PR722、およびL17から選択され る請求の範囲第13項記載の方法。
- 18.該ポリメラーゼが、対応する天然に存在するポリメラーゼによって呈され る天然に存在するエキソヌクレアーゼ活性の10%末満を呈する請求の範囲第1 3項記載の方法。
- 19.該DNAポリメラーゼが検出可能なエキソヌクレアーゼ活性を有しない請 求の範囲第13項記載の方法。
- 20.鋳型DNA分子を供し; プライマーを該鋳型分子と共にアニールし;φ29−タイプDNAポリメラーゼ および4種の異なるデオキシヌクレオシド三リン酸の混合物の存在下で、該アニ ールしたプライマーおよび鋳型分子をインキュベートすることを特徴とする10 キロベース長を超えるDNA分子の生産方法。
- 21.φ29−タイプ末端DNA配列をDNA分子の一端に共有的に結合させて 産物を形成させ;次いで、 φ29−タイプDNAポリメラーゼ、φ29−タイプDNAポリメラーゼの末端 蛋白および4種の異なるデオキシヌクレオシド三リン酸の存在下で該産物をアニ ールする工程よりなることを特徴とする異種DNA分子の増幅方法。
- 22.さらに、φ29−タイプ末端配列を該DNA分子の各端部に供する工程よ りなる請求の範囲第21項記載の方法。
- 23.該末端配列が500ヌクレオチド未満のDNA断片上に供される請求の範 囲第22項記載の方法。
- 24.該末端蛋白が、その中でφ29−タイプDNAポリメラーゼが天然で生じ るφ29−タイプファージの末端蛋白である請求の範囲第21項記載の方法。
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/328,462 US5001050A (en) | 1989-03-24 | 1989-03-24 | PHφ29 DNA polymerase |
PCT/US1990/001631 WO1991016446A1 (en) | 1989-03-24 | 1990-04-13 | In vitro dna synthesis reactions using phi 29 dna polymerase and a dna fragment encoding said polymerase |
Publications (2)
Publication Number | Publication Date |
---|---|
JPH05508302A true JPH05508302A (ja) | 1993-11-25 |
JP2907231B2 JP2907231B2 (ja) | 1999-06-21 |
Family
ID=23281085
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2508123A Expired - Fee Related JP2907231B2 (ja) | 1989-03-24 | 1990-04-13 | Ph129dnaポリメラーゼおよび該ポリメラーゼをコードするdna断片を用いるイン・ビトロdna合成反応 |
Country Status (6)
Country | Link |
---|---|
US (1) | US5001050A (ja) |
EP (1) | EP0527728B1 (ja) |
JP (1) | JP2907231B2 (ja) |
DE (1) | DE69031043T2 (ja) |
ES (1) | ES2103741T3 (ja) |
WO (1) | WO1991016446A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102015120225A1 (de) | 2014-12-02 | 2016-06-02 | Smc Corporation | Ionisator |
Families Citing this family (164)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5498523A (en) * | 1988-07-12 | 1996-03-12 | President And Fellows Of Harvard College | DNA sequencing with pyrophosphatase |
US5075216A (en) * | 1988-09-23 | 1991-12-24 | Cetus Corporation | Methods for dna sequencing with thermus aquaticus dna polymerase |
US5001050A (en) * | 1989-03-24 | 1991-03-19 | Consejo Superior Investigaciones Cientificas | PHφ29 DNA polymerase |
US5198543A (en) * | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
US5352778A (en) * | 1990-04-26 | 1994-10-04 | New England Biolabs, Inc. | Recombinant thermostable DNA polymerase from archaebacteria |
US5756334A (en) * | 1990-04-26 | 1998-05-26 | New England Biolabs, Inc. | Thermostable DNA polymerase from 9°N-7 and methods for producing the same |
US5322785A (en) * | 1990-04-26 | 1994-06-21 | New England Biolabs, Inc. | Purified thermostable DNA polymerase obtainable from thermococcus litoralis |
US5500363A (en) * | 1990-04-26 | 1996-03-19 | New England Biolabs, Inc. | Recombinant thermostable DNA polymerase from archaebacteria |
WO1992001813A1 (en) * | 1990-07-25 | 1992-02-06 | Syngene, Inc. | Circular extension for generating multiple nucleic acid complements |
US5861504A (en) * | 1991-05-29 | 1999-01-19 | The United States Of America As Represented By The Department Of Health And Human Services | Eleven highly informative microsatelite repeat polymorphic DNA markers |
US5378602A (en) * | 1991-05-29 | 1995-01-03 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Highly informative microsatellite repeat polymorphic DNA markers twenty-[seven]six |
US5468610A (en) * | 1991-05-29 | 1995-11-21 | The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Three highly informative microsatellite repeat polymorphic DNA markers |
USH1985H1 (en) | 1992-01-09 | 2001-08-07 | The United States Of America As Represented By The Secretary Of The Navy | Method for detecting biological toxins |
US5830642A (en) * | 1992-04-03 | 1998-11-03 | Amersham Life Science, Inc. | Electrophoresis of nucleic acid fragments |
US5436149A (en) * | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
US7465539B1 (en) | 1993-02-19 | 2008-12-16 | Takara Bio, Inc. | DNA polymerases with enhanced length of primer extension |
US6410277B1 (en) | 1993-02-19 | 2002-06-25 | Takara Shuzo Co., Ltd. | DNA polymersases with enhanced length of primer extension |
US5547859A (en) * | 1993-08-02 | 1996-08-20 | Goodman; Myron F. | Chain-terminating nucleotides for DNA sequencing methods |
US7312052B1 (en) * | 1993-12-08 | 2007-12-25 | Stratagene California | Polymerase compositions and uses thereof |
US5512462A (en) * | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
US6015668A (en) * | 1994-09-30 | 2000-01-18 | Life Technologies, Inc. | Cloned DNA polymerases from thermotoga and mutants thereof |
DE655506T1 (de) * | 1994-10-17 | 1995-09-28 | Harvard College | DNS Polymerase mit Veränderten Nukleotiden Bindungstelle. |
US5614365A (en) * | 1994-10-17 | 1997-03-25 | President & Fellow Of Harvard College | DNA polymerase having modified nucleotide binding site for DNA sequencing |
AU707608B2 (en) * | 1995-02-01 | 1999-07-15 | Research Development Foundation | Detecting DNA damage, measuring DNA repair rates, and monitoring DNA repair enzyme activity |
US5633159A (en) * | 1995-03-10 | 1997-05-27 | Becton, Dickinson And Company | DNA polymerase III β-subunit from mycobacteriophage DS6A |
AU7236296A (en) * | 1995-09-08 | 1997-03-27 | Life Technologies, Inc. | Cloned dna polymerases from thermotoga and mutants thereof |
US5854033A (en) * | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
JP3974941B2 (ja) | 1995-11-21 | 2007-09-12 | イェール ユニバーシティ | 単分子セグメントの増幅および検出 |
US20080160524A1 (en) * | 1996-01-24 | 2008-07-03 | Third Wave Technologies, Inc. | Methods and Compositions for Detecting Target Sequences |
US7195871B2 (en) * | 1996-01-24 | 2007-03-27 | Third Wave Technologies, Inc | Methods and compositions for detecting target sequences |
US6291164B1 (en) | 1996-11-22 | 2001-09-18 | Invitrogen Corporation | Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate |
US6306588B1 (en) | 1997-02-07 | 2001-10-23 | Invitrogen Corporation | Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof |
JP3935509B2 (ja) * | 1997-02-12 | 2007-06-27 | ワイ. チャン,ユージーン | ポリマー分析のための方法および製品 |
JP4245084B2 (ja) | 1997-03-21 | 2009-03-25 | ストラタジーン | ポリメラーゼ増強因子(pef)抽出物、pefタンパク質複合体、単離されたpefタンパク質、並びに精製及び単離方法 |
US6124120A (en) | 1997-10-08 | 2000-09-26 | Yale University | Multiple displacement amplification |
EP1098996A1 (en) | 1998-07-20 | 2001-05-16 | Yale University | Method for detecting nucleic acids using target-mediated ligation of bipartite primers |
WO2000015849A1 (en) | 1998-09-15 | 2000-03-23 | Yale University | Artificial long terminal repeat vectors |
JP2002525049A (ja) | 1998-09-15 | 2002-08-13 | イェール ユニバーシティ | ローリングサークル増幅を用いる分子クローニング |
US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
US20030215821A1 (en) * | 1999-04-20 | 2003-11-20 | Kevin Gunderson | Detection of nucleic acid reactions on bead arrays |
US6291187B1 (en) | 2000-05-12 | 2001-09-18 | Molecular Staging, Inc. | Poly-primed amplification of nucleic acid sequences |
US6531283B1 (en) * | 2000-06-20 | 2003-03-11 | Molecular Staging, Inc. | Protein expression profiling |
US20060166227A1 (en) * | 2000-06-20 | 2006-07-27 | Stephen Kingsmore | Protein expression profiling |
US6323009B1 (en) * | 2000-06-28 | 2001-11-27 | Molecular Staging, Inc. | Multiply-primed amplification of nucleic acid sequences |
DE60130763T2 (de) | 2000-06-30 | 2008-07-17 | Qiagen Gmbh | Signalamplifikation mit "lollipop"-hydridisierungsproben |
US7198924B2 (en) | 2000-12-11 | 2007-04-03 | Invitrogen Corporation | Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites |
WO2002061133A2 (en) * | 2000-11-09 | 2002-08-08 | Yale University | Nucleic acid detection using structured probes |
US20080261220A1 (en) * | 2000-11-30 | 2008-10-23 | Third Wave Technologies, Inc. | Nucleic Acid Detection Assays |
AU2002227156A1 (en) * | 2000-12-01 | 2002-06-11 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
US6573051B2 (en) * | 2001-03-09 | 2003-06-03 | Molecular Staging, Inc. | Open circle probes with intramolecular stem structures |
US20030152942A1 (en) * | 2001-05-09 | 2003-08-14 | Lance Fors | Nucleic acid detection in pooled samples |
US7668697B2 (en) * | 2006-02-06 | 2010-02-23 | Andrei Volkov | Method for analyzing dynamic detectable events at the single molecule level |
US6617137B2 (en) | 2001-10-15 | 2003-09-09 | Molecular Staging Inc. | Method of amplifying whole genomes without subjecting the genome to denaturing conditions |
US7297485B2 (en) * | 2001-10-15 | 2007-11-20 | Qiagen Gmbh | Method for nucleic acid amplification that results in low amplification bias |
US6977148B2 (en) * | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
US7553619B2 (en) | 2002-02-08 | 2009-06-30 | Qiagen Gmbh | Detection method using dissociated rolling circle amplification |
US20040197802A1 (en) | 2002-11-21 | 2004-10-07 | Dahl Gary A. | Methods for using primers that encode one strand of a double-stranded promoter |
JP4886298B2 (ja) | 2002-12-20 | 2012-02-29 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 核酸増幅 |
US7955795B2 (en) * | 2003-06-06 | 2011-06-07 | Qiagen Gmbh | Method of whole genome amplification with reduced artifact production |
US9487823B2 (en) * | 2002-12-20 | 2016-11-08 | Qiagen Gmbh | Nucleic acid amplification |
US6977153B2 (en) * | 2002-12-31 | 2005-12-20 | Qiagen Gmbh | Rolling circle amplification of RNA |
US6943768B2 (en) | 2003-02-21 | 2005-09-13 | Xtellus Inc. | Thermal control system for liquid crystal cell |
US8043834B2 (en) | 2003-03-31 | 2011-10-25 | Qiagen Gmbh | Universal reagents for rolling circle amplification and methods of use |
US20040259100A1 (en) | 2003-06-20 | 2004-12-23 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
US20050095627A1 (en) * | 2003-09-03 | 2005-05-05 | The Salk Institute For Biological Studies | Multiple antigen detection assays and reagents |
ATE469984T1 (de) | 2003-12-01 | 2010-06-15 | Life Technologies Corp | Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon |
US20050136414A1 (en) * | 2003-12-23 | 2005-06-23 | Kevin Gunderson | Methods and compositions for making locus-specific arrays |
US20060024711A1 (en) * | 2004-07-02 | 2006-02-02 | Helicos Biosciences Corporation | Methods for nucleic acid amplification and sequence determination |
US20060216724A1 (en) * | 2004-07-30 | 2006-09-28 | Affymetrix, Inc. | Methods for normalized amplification of nucleic acids |
US7476503B2 (en) * | 2004-09-17 | 2009-01-13 | Pacific Biosciences Of California, Inc. | Apparatus and method for performing nucleic acid analysis |
US7170050B2 (en) * | 2004-09-17 | 2007-01-30 | Pacific Biosciences Of California, Inc. | Apparatus and methods for optical analysis of molecules |
EP1819828A2 (en) * | 2004-12-11 | 2007-08-22 | CytoGenix, Inc. | Cell free biosynthesis of high-quality nucleic acid and uses thereof |
US20060252032A1 (en) * | 2005-01-28 | 2006-11-09 | Third Wave Technologies, Inc. | Detection of human herpesviruses |
EP1863908B1 (de) * | 2005-04-01 | 2010-11-17 | Qiagen GmbH | Reverse transkription und amplifikation von rna bei simultaner degradierung von dna |
EP1762627A1 (de) | 2005-09-09 | 2007-03-14 | Qiagen GmbH | Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion |
US7763423B2 (en) * | 2005-09-30 | 2010-07-27 | Pacific Biosciences Of California, Inc. | Substrates having low density reactive groups for monitoring enzyme activity |
JP2009511019A (ja) * | 2005-10-06 | 2009-03-19 | ルシジェン コーポレイション | 耐熱性ウィルス性ポリメラーゼ及びその使用法 |
US7482127B2 (en) * | 2005-11-03 | 2009-01-27 | Third Wave Technologies, Inc. | Nucleic acid detection assays employing blocker oligonucleotides |
WO2007076057A2 (en) | 2005-12-22 | 2007-07-05 | Pacific Biosciences Of California, Inc. | Polymerases for nucleotide analogue incorporation |
AU2006331512B2 (en) * | 2005-12-22 | 2012-02-23 | Pacific Biosciences Of California, Inc. | Active surface coupled polymerases |
US20080057499A1 (en) * | 2006-02-06 | 2008-03-06 | Affymetrix, Inc. | Methods for high specificity whole genome amplification and hybridization |
US20080050747A1 (en) * | 2006-03-30 | 2008-02-28 | Pacific Biosciences Of California, Inc. | Articles having localized molecules disposed thereon and methods of producing and using same |
US8975216B2 (en) * | 2006-03-30 | 2015-03-10 | Pacific Biosciences Of California | Articles having localized molecules disposed thereon and methods of producing same |
DE102006020885A1 (de) * | 2006-05-05 | 2007-11-08 | Qiagen Gmbh | Einführung von Sequenzelementen in Nukleinsäuren |
US20080131875A1 (en) * | 2006-06-07 | 2008-06-05 | Third Wave Technologies, Inc. | Multiplex assays |
US7759062B2 (en) * | 2006-06-09 | 2010-07-20 | Third Wave Technologies, Inc. | T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection |
WO2008045251A2 (en) | 2006-10-04 | 2008-04-17 | Third Wave Technologies, Inc. | Snap-back primers and detectable hairpin structures |
US8343746B2 (en) * | 2006-10-23 | 2013-01-01 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
US20080096258A1 (en) * | 2006-10-24 | 2008-04-24 | Christian Korfhage | Rolling circle amplification of circular genomes |
US20080242560A1 (en) * | 2006-11-21 | 2008-10-02 | Gunderson Kevin L | Methods for generating amplified nucleic acid arrays |
US8551704B2 (en) | 2007-02-16 | 2013-10-08 | Pacific Biosciences Of California, Inc. | Controllable strand scission of mini circle DNA |
WO2009017678A2 (en) | 2007-07-26 | 2009-02-05 | Pacific Biosciences Of California, Inc. | Molecular redundant sequencing |
EP2173909A1 (en) * | 2007-07-26 | 2010-04-14 | Roche Diagnostics GmbH | Target preparation for parallel sequencing of complex genomes |
US7960116B2 (en) | 2007-09-28 | 2011-06-14 | Pacific Biosciences Of California, Inc. | Nucleic acid sequencing methods and systems |
CA2700838A1 (en) * | 2007-09-28 | 2009-04-09 | Pacific Biosciences Of California, Inc. | Error-free amplification of dna for clonal sequencing |
US8652781B2 (en) | 2008-02-12 | 2014-02-18 | Pacific Biosciences Of California, Inc. | Cognate sampling kinetics |
MX2010010161A (es) * | 2008-03-15 | 2011-03-21 | Hologic Inc Star | Composiciones y metodos para el analisis de moleculas de acido nucleico durante reacciones de amplificacion. |
US8628940B2 (en) | 2008-09-24 | 2014-01-14 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
US8153375B2 (en) | 2008-03-28 | 2012-04-10 | Pacific Biosciences Of California, Inc. | Compositions and methods for nucleic acid sequencing |
US8143030B2 (en) * | 2008-09-24 | 2012-03-27 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
WO2009120374A2 (en) | 2008-03-28 | 2009-10-01 | Pacific Biosciences Of California, Inc. | Methods and compositions for nucleic acid sample preparation |
US20090247426A1 (en) * | 2008-03-31 | 2009-10-01 | Pacific Biosciences Of California, Inc. | Focused library generation |
US8420366B2 (en) * | 2008-03-31 | 2013-04-16 | Pacific Biosciences Of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
EP2942404B1 (en) | 2008-03-31 | 2016-11-23 | Pacific Biosciences of California, Inc. | Generation of modified polymerases for improved accuracy in single molecule sequencing |
US8999676B2 (en) | 2008-03-31 | 2015-04-07 | Pacific Biosciences Of California, Inc. | Recombinant polymerases for improved single molecule sequencing |
WO2009145828A2 (en) * | 2008-03-31 | 2009-12-03 | Pacific Biosciences Of California, Inc. | Two slow-step polymerase enzyme systems and methods |
WO2010012002A1 (en) * | 2008-07-25 | 2010-01-28 | Saryna Medical Corporation | Methods and systems for genetic analysis of fetal nucleated red blood cells |
US8795961B2 (en) * | 2008-09-05 | 2014-08-05 | Pacific Biosciences Of California, Inc. | Preparations, compositions, and methods for nucleic acid sequencing |
EP2326733A2 (en) * | 2008-09-05 | 2011-06-01 | Pacific Biosciences of California, Inc. | Engineering polymerases and reaction conditions for modified incorporation properties |
US8383369B2 (en) | 2008-09-24 | 2013-02-26 | Pacific Biosciences Of California, Inc. | Intermittent detection during analytical reactions |
WO2010111690A2 (en) | 2009-03-27 | 2010-09-30 | Life Technologies Corporation | Polymerase compositions and methods |
US8632975B2 (en) | 2009-06-05 | 2014-01-21 | Life Technologies Corporation | Nucleotide transient binding for sequencing methods |
ES2351294B8 (es) | 2009-07-02 | 2012-06-06 | Consejo Superior De Investigaciones Científicas (Csic) | Metodo para la replicación, amplificación, o secuenciación de un adn molde. |
ES2359058B1 (es) | 2009-07-02 | 2012-03-27 | Consejo Superior De Investigaciones Cient�?Ficas (Csic) | Quimera de adn polimerasa del fago ph1 29. |
US8501406B1 (en) | 2009-07-14 | 2013-08-06 | Pacific Biosciences Of California, Inc. | Selectively functionalized arrays |
JP2013500713A (ja) | 2009-07-31 | 2013-01-10 | サントル オスピタリエ ウニヴェルシテール ヴォドア | Hcv感染患者においてc型肝炎の転帰を診断又は予測する方法 |
GB0922377D0 (en) | 2009-12-22 | 2010-02-03 | Arab Gulf University The | Mutant LDL receptor |
GB201001088D0 (en) | 2010-01-23 | 2010-03-10 | Trillion Genomics Ltd | Detection |
EP2357225A1 (en) | 2010-02-11 | 2011-08-17 | Institut Pasteur | Thermostable primase/DNA polymerase of the Thermococcus nautilus 30/1 plasmid pTN2 and its applications |
EP2808401B1 (en) | 2010-02-26 | 2016-12-14 | Life Technologies Corporation | Method for sequencing using a modified DNA polymerase |
US8774488B2 (en) | 2010-03-11 | 2014-07-08 | Cellscape Corporation | Method and device for identification of nucleated red blood cells from a maternal blood sample |
WO2012009206A2 (en) | 2010-07-12 | 2012-01-19 | Pacific Biosciences Of California, Inc. | Sequencing reactions with alkali metal cations for pulse width control |
US9121058B2 (en) | 2010-08-20 | 2015-09-01 | Integenx Inc. | Linear valve arrays |
US8936911B2 (en) | 2010-09-22 | 2015-01-20 | Pacific Biosciences Of California, Inc. | Purified extended polymerase/template complex for sequencing |
WO2012039964A1 (en) | 2010-09-24 | 2012-03-29 | Wisconsin Alumni Research Foundation | Compositions and methods for predicting hcv susceptibility to antiviral agents |
WO2012129242A2 (en) | 2011-03-23 | 2012-09-27 | Pacific Biosciences Of California, Inc. | Isolation of polymerase-nucleic acid complexes and loading onto substrates |
EP2726632A1 (en) | 2011-06-30 | 2014-05-07 | Centre Hospitaller Universitaire Vaudois (CHUV) | Polymorphisms associated with non-response to a hepatitis c treatment or susceptibility to non-spontaneous hepatitis c clearance |
WO2013030786A1 (en) | 2011-08-31 | 2013-03-07 | Centre Hospitalier Universitaire Vaudois | Method for diagnosing or predicting hepatocellular carcinoma outcome |
WO2013052862A1 (en) | 2011-10-05 | 2013-04-11 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Genetic marker for predicting prognosis in patients infected with hepatitis c virus |
US9347900B2 (en) | 2011-10-14 | 2016-05-24 | Pacific Biosciences Of California, Inc. | Real-time redox sequencing |
US8906660B2 (en) | 2012-02-01 | 2014-12-09 | Pacific Biosciences Of California, Inc. | Recombinant polymerases with increased phototolerance |
EP2814953B1 (en) | 2012-02-15 | 2017-06-07 | Pacific Biosciences Of California, Inc. | Polymerase enzyme substrates with protein shield |
WO2013185137A1 (en) | 2012-06-08 | 2013-12-12 | Pacific Biosciences Of California, Inc. | Modified base detection with nanopore sequencing |
EP4071253A1 (en) | 2012-07-26 | 2022-10-12 | Illumina, Inc. | Compositions and methods for the amplification of nucleic acids |
US9399766B2 (en) | 2012-10-01 | 2016-07-26 | Pacific Biosciences Of California, Inc. | Recombinant polymerases for incorporation of protein shield nucleotide analogs |
EP2722399A1 (en) * | 2012-10-18 | 2014-04-23 | Roche Diagniostics GmbH | Method for preventing high molecular weight products during amplification |
US9290800B2 (en) | 2013-03-15 | 2016-03-22 | Pacific Biosciences Of California, Inc. | Targeted rolling circle amplification |
JP6587544B2 (ja) | 2013-03-15 | 2019-10-09 | シグニス バイオテク エセ.エレ.ウ.Sygnis Biotech S.L.U. | 熱安定なTthPrimPolを用いた増幅及び配列決定方法 |
JP2016515827A (ja) | 2013-04-04 | 2016-06-02 | ジョージア ステイト ユニバーシティ リサーチ ファンデーション, インコーポレイテッド | ナノ粒子支援シグナル増幅を使用するrnaマイクロチップ検出 |
CN105378113B (zh) | 2013-05-06 | 2020-02-21 | 加利福尼亚太平洋生物科学股份有限公司 | 实时电子测序 |
US10480021B2 (en) | 2014-06-23 | 2019-11-19 | Yale University | Methods for closed chromatin mapping and DNA methylation analysis for single cells |
US10302972B2 (en) | 2015-01-23 | 2019-05-28 | Pacific Biosciences Of California, Inc. | Waveguide transmission |
EP3274473B1 (en) | 2015-03-24 | 2020-10-28 | Pacific Biosciences of California, Inc. | Methods and compositions for single molecule composition loading |
EP3298170B1 (en) | 2015-05-21 | 2021-12-29 | Cofactor Genomics, Inc. | Methods for generating circular dna from circular rna |
US10280411B2 (en) | 2015-10-27 | 2019-05-07 | Pacific Biosciences of California, In.c | Methods, systems, and reagents for direct RNA sequencing |
CN117024498A (zh) | 2015-11-20 | 2023-11-10 | 加利福尼亚太平洋生物科学股份有限公司 | 受保护的染料标记的试剂 |
WO2017087975A1 (en) | 2015-11-20 | 2017-05-26 | Pacific Biosciences Of California, Inc. | Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing |
US11401548B2 (en) | 2016-03-25 | 2022-08-02 | Qiagen Sciences, Llc | Primers with self-complementary sequences for multiple displacement amplification |
US10894990B2 (en) | 2016-05-17 | 2021-01-19 | Shoreline Biome, Llc | High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures |
US10544457B2 (en) | 2016-06-14 | 2020-01-28 | Pacific Biosciences Of California, Inc. | Methods and compositions for enriching compositions for polymerase enzyme complexes |
CN109477142B (zh) | 2016-07-18 | 2022-03-22 | 豪夫迈·罗氏有限公司 | 不对称模板和核酸测序的不对称方法 |
ES2875318T3 (es) | 2016-07-18 | 2021-11-10 | Hoffmann La Roche | Procedimiento para generar colecciones de ADN circular monocatenario para secuenciación de molécula única |
US10711300B2 (en) | 2016-07-22 | 2020-07-14 | Pacific Biosciences Of California, Inc. | Methods and compositions for delivery of molecules and complexes to reaction sites |
WO2019053132A1 (en) | 2017-09-14 | 2019-03-21 | F. Hoffmann-La Roche Ag | NEW METHOD FOR THE GENERATION OF CIRCULAR SINGLE STRAND DNA LIBRARIES |
EP3682027A1 (en) | 2017-09-15 | 2020-07-22 | H. Hoffnabb-La Roche Ag | Hybridization-extension-ligation strategy for generating circular single-stranded dna libraries |
EP3668998A1 (en) | 2017-10-06 | 2020-06-24 | Cartana AB | Rna templated ligation |
JP7083393B2 (ja) | 2017-10-06 | 2022-06-10 | エフ.ホフマン-ラ ロシュ アーゲー | 単一分子配列決定の試料調製のための環化方法 |
US11162138B2 (en) | 2017-10-30 | 2021-11-02 | Pacific Biosciences Of California, Inc. | Multi-amplitude modular labeled compounds |
CN112041459A (zh) | 2018-01-29 | 2020-12-04 | 圣祖德儿童研究医院 | 核酸扩增方法 |
WO2019209946A1 (en) | 2018-04-25 | 2019-10-31 | Qiagen Sciences Llc | Sequential paired-end sequencing |
EP3674702A1 (en) | 2018-12-27 | 2020-07-01 | Imec VZW | Method for sequencing a polynucleotide using a biofet |
WO2021197610A1 (en) | 2020-04-03 | 2021-10-07 | Centre National De La Recherche Scientifique - Cnrs - | Molecular experimentation device, apparatus and method |
WO2021197618A1 (en) | 2020-04-03 | 2021-10-07 | Centre National De La Recherche Scientifique - Cnrs - | Flash nucleic acid sequencing |
WO2024015962A1 (en) | 2022-07-15 | 2024-01-18 | Pacific Biosciences Of California, Inc. | Blocked asymmetric hairpin adaptors |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4946786A (en) * | 1987-01-14 | 1990-08-07 | President And Fellows Of Harvard College | T7 DNA polymerase |
US4921794A (en) * | 1987-01-14 | 1990-05-01 | President And Fellows Of Harvard College | T7 DNA polymerase |
US4942130A (en) * | 1987-01-14 | 1990-07-17 | President & Fellows Of Harvard College | T7 DNA polymerase |
US4795699A (en) * | 1987-01-14 | 1989-01-03 | President And Fellows Of Harvard College | T7 DNA polymerase |
DK0593095T3 (da) * | 1989-03-03 | 2005-10-17 | Genentech Inc | Genomisk kloning og kortlægning |
US5001050A (en) * | 1989-03-24 | 1991-03-19 | Consejo Superior Investigaciones Cientificas | PHφ29 DNA polymerase |
HUT61054A (en) * | 1989-04-12 | 1992-11-30 | Harvard College | Improved process and test kit for primary extension reactions |
DE3929030A1 (de) * | 1989-09-01 | 1991-03-07 | Boehringer Mannheim Gmbh | Verfahren zur vermehrung von nukleinsaeuren |
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1989
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DE102015120225A1 (de) | 2014-12-02 | 2016-06-02 | Smc Corporation | Ionisator |
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EP0527728A1 (en) | 1993-02-24 |
JP2907231B2 (ja) | 1999-06-21 |
DE69031043T2 (de) | 1997-10-30 |
EP0527728A4 (en) | 1994-11-23 |
US5001050A (en) | 1991-03-19 |
ES2103741T3 (es) | 1997-10-01 |
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