DE69031043D1 - In vitro-dns-synthesereaktionen unter verwendung von geändertem phi-29-dns-polymerase und für besagte polymerase kodierendes dns-bruchstück - Google Patents

In vitro-dns-synthesereaktionen unter verwendung von geändertem phi-29-dns-polymerase und für besagte polymerase kodierendes dns-bruchstück

Info

Publication number
DE69031043D1
DE69031043D1 DE69031043T DE69031043T DE69031043D1 DE 69031043 D1 DE69031043 D1 DE 69031043D1 DE 69031043 T DE69031043 T DE 69031043T DE 69031043 T DE69031043 T DE 69031043T DE 69031043 D1 DE69031043 D1 DE 69031043D1
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DE
Germany
Prior art keywords
dna
polymerase
phi
vitro
changed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69031043T
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English (en)
Other versions
DE69031043T2 (de
Inventor
Luis Avenida Prado De L Blanco
Antonio Calle Rocafort Bernad
Margarita Salas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Consejo Superior de Investigaciones Cientificas CSIC
Global Life Sciences Solutions USA LLC
Original Assignee
Consejo Superior de Investigaciones Cientificas CSIC
United States Biochemical Corp
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=23281085&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE69031043(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Consejo Superior de Investigaciones Cientificas CSIC, United States Biochemical Corp filed Critical Consejo Superior de Investigaciones Cientificas CSIC
Application granted granted Critical
Publication of DE69031043D1 publication Critical patent/DE69031043D1/de
Publication of DE69031043T2 publication Critical patent/DE69031043T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
    • Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69031043T 1989-03-24 1990-04-13 In vitro-dns-synthesereaktionen unter verwendung von geändertem phi-29-dns-polymerase und für besagte polymerase kodierendes dns-bruchstück Expired - Lifetime DE69031043T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/328,462 US5001050A (en) 1989-03-24 1989-03-24 PHφ29 DNA polymerase
PCT/US1990/001631 WO1991016446A1 (en) 1989-03-24 1990-04-13 In vitro dna synthesis reactions using phi 29 dna polymerase and a dna fragment encoding said polymerase

Publications (2)

Publication Number Publication Date
DE69031043D1 true DE69031043D1 (de) 1997-08-14
DE69031043T2 DE69031043T2 (de) 1997-10-30

Family

ID=23281085

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69031043T Expired - Lifetime DE69031043T2 (de) 1989-03-24 1990-04-13 In vitro-dns-synthesereaktionen unter verwendung von geändertem phi-29-dns-polymerase und für besagte polymerase kodierendes dns-bruchstück

Country Status (6)

Country Link
US (1) US5001050A (de)
EP (1) EP0527728B1 (de)
JP (1) JP2907231B2 (de)
DE (1) DE69031043T2 (de)
ES (1) ES2103741T3 (de)
WO (1) WO1991016446A1 (de)

Families Citing this family (165)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5498523A (en) * 1988-07-12 1996-03-12 President And Fellows Of Harvard College DNA sequencing with pyrophosphatase
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5198543A (en) * 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5352778A (en) * 1990-04-26 1994-10-04 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5322785A (en) * 1990-04-26 1994-06-21 New England Biolabs, Inc. Purified thermostable DNA polymerase obtainable from thermococcus litoralis
US5500363A (en) * 1990-04-26 1996-03-19 New England Biolabs, Inc. Recombinant thermostable DNA polymerase from archaebacteria
US5756334A (en) * 1990-04-26 1998-05-26 New England Biolabs, Inc. Thermostable DNA polymerase from 9°N-7 and methods for producing the same
AU649066B2 (en) * 1990-07-25 1994-05-12 Syngene, Inc. Circular extension for generating multiple nucleic acid complements
US5468610A (en) * 1991-05-29 1995-11-21 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Three highly informative microsatellite repeat polymorphic DNA markers
US5861504A (en) * 1991-05-29 1999-01-19 The United States Of America As Represented By The Department Of Health And Human Services Eleven highly informative microsatelite repeat polymorphic DNA markers
US5378602A (en) * 1991-05-29 1995-01-03 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Highly informative microsatellite repeat polymorphic DNA markers twenty-[seven]six
USH1985H1 (en) 1992-01-09 2001-08-07 The United States Of America As Represented By The Secretary Of The Navy Method for detecting biological toxins
US5830642A (en) * 1992-04-03 1998-11-03 Amersham Life Science, Inc. Electrophoresis of nucleic acid fragments
US5436149A (en) * 1993-02-19 1995-07-25 Barnes; Wayne M. Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension
US6410277B1 (en) 1993-02-19 2002-06-25 Takara Shuzo Co., Ltd. DNA polymersases with enhanced length of primer extension
US7465539B1 (en) 1993-02-19 2008-12-16 Takara Bio, Inc. DNA polymerases with enhanced length of primer extension
US5547859A (en) * 1993-08-02 1996-08-20 Goodman; Myron F. Chain-terminating nucleotides for DNA sequencing methods
US7312052B1 (en) * 1993-12-08 2007-12-25 Stratagene California Polymerase compositions and uses thereof
US5512462A (en) * 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
DE655506T1 (de) * 1994-10-17 1995-09-28 President And Fellows Of Harvard College, Cambridge, Mass. DNS Polymerase mit Veränderten Nukleotiden Bindungstelle.
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
CN1116421C (zh) * 1995-02-01 2003-07-30 研究发展基金会 检测dna损伤、测定dna修复速度和监测dna修复酶活性
US5633159A (en) * 1995-03-10 1997-05-27 Becton, Dickinson And Company DNA polymerase III β-subunit from mycobacteriophage DS6A
EP0871775A4 (de) * 1995-09-08 2002-07-31 Life Technologies Inc Klonierte dna-polymerasen von thermotoga und mutanten davon
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
DK0862656T3 (da) 1995-11-21 2001-04-09 Univ Yale Unimolekylær segmentamplifikation og -detektering
US20080160524A1 (en) * 1996-01-24 2008-07-03 Third Wave Technologies, Inc. Methods and Compositions for Detecting Target Sequences
US7195871B2 (en) * 1996-01-24 2007-03-27 Third Wave Technologies, Inc Methods and compositions for detecting target sequences
US6291164B1 (en) 1996-11-22 2001-09-18 Invitrogen Corporation Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
CA2281205A1 (en) * 1997-02-12 1998-08-13 Eugene Y. Chan Methods and products for analyzing polymers
EP1007718B1 (de) 1997-03-21 2007-11-14 Stratagene California Polymerase-verbessernder faktor (pef)-enthaltende extrakte, pef proteinkomplexe, isoliertes pef protein und verfahren zur reinigung und identifizierung
US6124120A (en) * 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6316229B1 (en) 1998-07-20 2001-11-13 Yale University Single molecule analysis target-mediated ligation of bipartite primers
JP2002525049A (ja) * 1998-09-15 2002-08-13 イェール ユニバーシティ ローリングサークル増幅を用いる分子クローニング
JP2002525078A (ja) 1998-09-15 2002-08-13 イェール ユニバーシティ 人工的長末端反復配列
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20030207295A1 (en) * 1999-04-20 2003-11-06 Kevin Gunderson Detection of nucleic acid reactions on bead arrays
US6291187B1 (en) 2000-05-12 2001-09-18 Molecular Staging, Inc. Poly-primed amplification of nucleic acid sequences
US6531283B1 (en) 2000-06-20 2003-03-11 Molecular Staging, Inc. Protein expression profiling
US20060166227A1 (en) * 2000-06-20 2006-07-27 Stephen Kingsmore Protein expression profiling
US6323009B1 (en) * 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
ATE374835T1 (de) 2000-06-30 2007-10-15 Qiagen Gmbh SIGNALAMPLIFIKATION MIT ßLOLLIPOPß- HYDRIDISIERUNGSPROBEN
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2002061133A2 (en) * 2000-11-09 2002-08-08 Yale University Nucleic acid detection using structured probes
US20080261220A1 (en) * 2000-11-30 2008-10-23 Third Wave Technologies, Inc. Nucleic Acid Detection Assays
EP1354064A2 (de) * 2000-12-01 2003-10-22 Visigen Biotechnologies, Inc. Enzymatische nukleinsäuresynthese: zusammensetzungen und verfahren, um die zuverlässigkeit des monomereinbaus zu erhöhen
US6573051B2 (en) 2001-03-09 2003-06-03 Molecular Staging, Inc. Open circle probes with intramolecular stem structures
AU2002311897A1 (en) * 2001-05-09 2002-11-18 Third Wave Technologies, Inc. Nucleic acid detection in pooled samples
US7668697B2 (en) * 2006-02-06 2010-02-23 Andrei Volkov Method for analyzing dynamic detectable events at the single molecule level
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
US6977148B2 (en) * 2001-10-15 2005-12-20 Qiagen Gmbh Multiple displacement amplification
US7297485B2 (en) * 2001-10-15 2007-11-20 Qiagen Gmbh Method for nucleic acid amplification that results in low amplification bias
US7553619B2 (en) 2002-02-08 2009-06-30 Qiagen Gmbh Detection method using dissociated rolling circle amplification
CN102268428A (zh) 2002-11-21 2011-12-07 震源技术公司 使用编码双链启动子一条链的引物的方法
US7955795B2 (en) * 2003-06-06 2011-06-07 Qiagen Gmbh Method of whole genome amplification with reduced artifact production
US9487823B2 (en) * 2002-12-20 2016-11-08 Qiagen Gmbh Nucleic acid amplification
EP2261371B1 (de) 2002-12-20 2015-07-22 QIAGEN GmbH Nukleinsäureamplifikation
US6977153B2 (en) * 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
US6943768B2 (en) 2003-02-21 2005-09-13 Xtellus Inc. Thermal control system for liquid crystal cell
US8043834B2 (en) 2003-03-31 2011-10-25 Qiagen Gmbh Universal reagents for rolling circle amplification and methods of use
US20040259100A1 (en) 2003-06-20 2004-12-23 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
WO2005074417A2 (en) * 2003-09-03 2005-08-18 Salk Institute For Biological Studies Multiple antigen detection assays and reagents
WO2005054438A2 (en) 2003-12-01 2005-06-16 Invitrogen Corporation Nucleic acid molecules containing recombination sites and methods of using the same
US20050136414A1 (en) * 2003-12-23 2005-06-23 Kevin Gunderson Methods and compositions for making locus-specific arrays
US20060024711A1 (en) * 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US20060216724A1 (en) * 2004-07-30 2006-09-28 Affymetrix, Inc. Methods for normalized amplification of nucleic acids
US7315019B2 (en) * 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
AU2005314431B2 (en) * 2004-12-11 2011-02-17 Cytogenix, Inc. Cell free biosynthesis of high-quality nucleic acid and uses thereof
US20060252032A1 (en) * 2005-01-28 2006-11-09 Third Wave Technologies, Inc. Detection of human herpesviruses
US8309303B2 (en) * 2005-04-01 2012-11-13 Qiagen Gmbh Reverse transcription and amplification of RNA with simultaneous degradation of DNA
EP1762627A1 (de) 2005-09-09 2007-03-14 Qiagen GmbH Verfahren zur Aktivierung einer Nukleinsäure für eine Polymerase-Reaktion
US7763423B2 (en) * 2005-09-30 2010-07-27 Pacific Biosciences Of California, Inc. Substrates having low density reactive groups for monitoring enzyme activity
EP2377929A1 (de) * 2005-10-06 2011-10-19 Lucigen Corporation Wärmestabile virale Polymerasen und Verwendungsverfahren
US7482127B2 (en) * 2005-11-03 2009-01-27 Third Wave Technologies, Inc. Nucleic acid detection assays employing blocker oligonucleotides
JP2009521227A (ja) * 2005-12-22 2009-06-04 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド ヌクレオチドアナログ組込み用ポリメラーゼ
CA2633476C (en) * 2005-12-22 2015-04-21 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
US20080057499A1 (en) * 2006-02-06 2008-03-06 Affymetrix, Inc. Methods for high specificity whole genome amplification and hybridization
US20080050747A1 (en) * 2006-03-30 2008-02-28 Pacific Biosciences Of California, Inc. Articles having localized molecules disposed thereon and methods of producing and using same
US8975216B2 (en) * 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
DE102006020885A1 (de) * 2006-05-05 2007-11-08 Qiagen Gmbh Einführung von Sequenzelementen in Nukleinsäuren
US20080131875A1 (en) * 2006-06-07 2008-06-05 Third Wave Technologies, Inc. Multiplex assays
US7759062B2 (en) * 2006-06-09 2010-07-20 Third Wave Technologies, Inc. T-structure invasive cleavage assays, consistent nucleic acid dispensing, and low level target nucleic acid detection
CA2665188C (en) 2006-10-04 2014-08-19 Third Wave Technologies, Inc. Snap-back primers and detectable hairpin structures
WO2008051530A2 (en) * 2006-10-23 2008-05-02 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US20080096258A1 (en) * 2006-10-24 2008-04-24 Christian Korfhage Rolling circle amplification of circular genomes
US20080242560A1 (en) * 2006-11-21 2008-10-02 Gunderson Kevin L Methods for generating amplified nucleic acid arrays
US8551704B2 (en) 2007-02-16 2013-10-08 Pacific Biosciences Of California, Inc. Controllable strand scission of mini circle DNA
CN101802220B (zh) 2007-07-26 2013-07-31 加利福尼亚太平洋生物科学股份有限公司 分子冗余测序法
EP2173909A1 (de) * 2007-07-26 2010-04-14 Roche Diagnostics GmbH Targetvorbereitung zur parallelen sequenzierung komplexer genome
US8003330B2 (en) * 2007-09-28 2011-08-23 Pacific Biosciences Of California, Inc. Error-free amplification of DNA for clonal sequencing
US7960116B2 (en) 2007-09-28 2011-06-14 Pacific Biosciences Of California, Inc. Nucleic acid sequencing methods and systems
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
CN102301000B (zh) * 2008-03-15 2015-04-29 豪洛捷公司 核酸分子扩增反应的分析组分及方法
EP3045542B1 (de) 2008-03-28 2016-11-16 Pacific Biosciences of California, Inc. Verfahren zur nukleinsäuresequenzierung
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8143030B2 (en) * 2008-09-24 2012-03-27 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
WO2009145828A2 (en) * 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc. Two slow-step polymerase enzyme systems and methods
US20090247426A1 (en) * 2008-03-31 2009-10-01 Pacific Biosciences Of California, Inc. Focused library generation
US8420366B2 (en) * 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
AU2009251883B2 (en) 2008-03-31 2014-09-11 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
WO2010012002A1 (en) * 2008-07-25 2010-01-28 Saryna Medical Corporation Methods and systems for genetic analysis of fetal nucleated red blood cells
WO2010027497A2 (en) * 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc Preparations, compositions, and methods for nucleic acid sequencing
AU2009288696A1 (en) * 2008-09-05 2010-03-11 Pacific Biosciences Of California, Inc. Sequencing by cognate sampling
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2010111686A2 (en) 2009-03-27 2010-09-30 Life Technologies Corp Labeled enzyme compositions, methods & systems
WO2010141390A2 (en) * 2009-06-05 2010-12-09 Life Technologies Corporation Nucleotide transient binding for sequencing methods
ES2359058B1 (es) 2009-07-02 2012-03-27 Consejo Superior De Investigaciones Cient�?Ficas (Csic) Quimera de adn polimerasa del fago ph1 29.
ES2351294B8 (es) 2009-07-02 2012-06-06 Consejo Superior De Investigaciones Científicas (Csic) Metodo para la replicación, amplificación, o secuenciación de un adn molde.
US8501406B1 (en) 2009-07-14 2013-08-06 Pacific Biosciences Of California, Inc. Selectively functionalized arrays
CN102665753A (zh) 2009-07-31 2012-09-12 佛多斯大学医学研究中心 诊断或预测hcv感染的患者中丙型肝炎后果的方法
GB0922377D0 (en) 2009-12-22 2010-02-03 Arab Gulf University The Mutant LDL receptor
GB201001088D0 (en) 2010-01-23 2010-03-10 Trillion Genomics Ltd Detection
EP2357225A1 (de) 2010-02-11 2011-08-17 Institut Pasteur Wärmebeständige Primase/DNA-Polymerase von Thermococcus nautilus 30/1-Plasmid pTN2 und deren Anwendungen
WO2011106634A2 (en) 2010-02-26 2011-09-01 Life Technologies Corporation Modified proteins and methods of making and using same
US8774488B2 (en) 2010-03-11 2014-07-08 Cellscape Corporation Method and device for identification of nucleated red blood cells from a maternal blood sample
WO2012009206A2 (en) 2010-07-12 2012-01-19 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
US9121058B2 (en) 2010-08-20 2015-09-01 Integenx Inc. Linear valve arrays
US8936911B2 (en) 2010-09-22 2015-01-20 Pacific Biosciences Of California, Inc. Purified extended polymerase/template complex for sequencing
US20120077738A1 (en) 2010-09-24 2012-03-29 Wisconsin Alumni Research Foundation Compositions and methods for predicting hcv susceptibility to antiviral agents
WO2012129242A2 (en) 2011-03-23 2012-09-27 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
WO2013001504A1 (en) 2011-06-30 2013-01-03 Centre Hospitalier Universitaire Vaudois (C.H.U.V.) Polymorphisms associated with non-response to a hepatitis c treatment or susceptibility to non-spontaneous hepatitis c clearance
WO2013030786A1 (en) 2011-08-31 2013-03-07 Centre Hospitalier Universitaire Vaudois Method for diagnosing or predicting hepatocellular carcinoma outcome
US20140271542A1 (en) 2011-10-05 2014-09-18 The United States Of America As Represented By The Secretary, Department Of Health And Human Service Genetic marker for predicting prognosis in patients infected with hepatitis c virus
WO2013056241A2 (en) 2011-10-14 2013-04-18 Pacific Biosciences Of California, Inc. Real-time redox sequencing
US8906660B2 (en) 2012-02-01 2014-12-09 Pacific Biosciences Of California, Inc. Recombinant polymerases with increased phototolerance
EP3590948A1 (de) 2012-02-15 2020-01-08 Pacific Biosciences of California, Inc. Polymeraseenzymsubstrate mit proteinabschirmung
GB2559073A (en) 2012-06-08 2018-07-25 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
ES2917400T3 (es) 2012-07-26 2022-07-08 Illumina Inc Composiciones y métodos para la amplificación de ácidos nucleicos
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
EP2722399A1 (de) * 2012-10-18 2014-04-23 Roche Diagniostics GmbH Verfahren zur Vermeidung von Produkten mit hohem Molekulargewicht während einer Amplifikation
DK2971080T3 (en) 2013-03-15 2018-02-12 Expedeon S L METHODS FOR AMPLIFICATION AND SEQUENCE USING THERMOSTABLE TTHPRIMPOL
US9290800B2 (en) 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
US20160068894A1 (en) 2013-04-04 2016-03-10 Georgia State University Research Foundation, Inc. RNA Microchip Detection Using Nanoparticle-Assisted Signal Amplification
EP3575414B1 (de) 2013-05-06 2023-09-06 Pacific Biosciences of California, Inc. Elektronische echtzeitsequenzierung
US10480021B2 (en) 2014-06-23 2019-11-19 Yale University Methods for closed chromatin mapping and DNA methylation analysis for single cells
JP6485684B2 (ja) 2014-12-02 2019-03-20 Smc株式会社 イオナイザ
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
WO2016154345A1 (en) 2015-03-24 2016-09-29 Pacific Biosciences Of California, Inc. Methods and compositions for single molecule composition loading
US20160338421A1 (en) 2015-05-21 2016-11-24 Marla Hope Caprow-D'Avanzo Post-breast surgery bra
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
WO2017087974A1 (en) 2015-11-20 2017-05-26 Pacific Biosciences Of California, Inc. Protected dye-labeled reagents
WO2017087975A1 (en) 2015-11-20 2017-05-26 Pacific Biosciences Of California, Inc. Labeled nucleotide analogs, reaction mixtures, and methods and systems for sequencing
EP3998351A1 (de) 2016-03-25 2022-05-18 Qiagen Sciences, LLC Primer mit einander ergänzenden sequenzen zur mehrfachen verlagerungsverstärkung
US10894990B2 (en) 2016-05-17 2021-01-19 Shoreline Biome, Llc High throughput method for identification and sequencing of unknown microbial and eukaryotic genomes from complex mixtures
US10544457B2 (en) 2016-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Methods and compositions for enriching compositions for polymerase enzyme complexes
CN109312391B (zh) 2016-07-18 2022-06-03 豪夫迈·罗氏有限公司 生成用于单分子测序的单链环状dna文库的方法
WO2018015365A1 (en) 2016-07-18 2018-01-25 Roche Sequencing Solutions, Inc. Asymmetric templates and asymmetric method of nucleic acid sequencing
US10711300B2 (en) 2016-07-22 2020-07-14 Pacific Biosciences Of California, Inc. Methods and compositions for delivery of molecules and complexes to reaction sites
JP2020532976A (ja) 2017-09-14 2020-11-19 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 環状一本鎖ｄｎａライブラリーを生成するための新規な方法
EP3682027A1 (de) 2017-09-15 2020-07-22 H. Hoffnabb-La Roche Ag Strategie zur hybridisierungverlängerungsligation zur erzeugung von bibliotheken aus zirkulärer einzelsträngiger dna
EP3668998A1 (de) 2017-10-06 2020-06-24 Cartana AB Rna-template-ligation
JP7083393B2 (ja) 2017-10-06 2022-06-10 エフ．ホフマン－ラ ロシュ アーゲー 単一分子配列決定の試料調製のための環化方法
US11162138B2 (en) 2017-10-30 2021-11-02 Pacific Biosciences Of California, Inc. Multi-amplitude modular labeled compounds
DK4183886T3 (da) 2018-01-29 2024-06-03 St Jude Childrens Res Hospital Inc Fremgangsmåde til nukleinsyreamplifikation
US20210189460A1 (en) 2018-04-25 2021-06-24 Qiagen Sciences Llc Sequential paired-end sequencing
EP3674702A1 (de) 2018-12-27 2020-07-01 Imec VZW Verfahren zur sequenzierung eines polynukleotids mittels eines biofet
WO2021197610A1 (en) 2020-04-03 2021-10-07 Centre National De La Recherche Scientifique - Cnrs - Molecular experimentation device, apparatus and method
WO2021197618A1 (en) 2020-04-03 2021-10-07 Centre National De La Recherche Scientifique - Cnrs - Flash nucleic acid sequencing
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4921794A (en) * 1987-01-14 1990-05-01 President And Fellows Of Harvard College T7 DNA polymerase
US4795699A (en) * 1987-01-14 1989-01-03 President And Fellows Of Harvard College T7 DNA polymerase
US4946786A (en) * 1987-01-14 1990-08-07 President And Fellows Of Harvard College T7 DNA polymerase
US4942130A (en) * 1987-01-14 1990-07-17 President & Fellows Of Harvard College T7 DNA polymerase
ES2058893T3 (es) * 1989-03-03 1994-11-01 Genentech Inc Procedimientos mejorados para la amplificacion de adn in vitro y para clonacion y cartografia de genomas.
US5001050A (en) * 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
KR920700294A (ko) * 1989-04-12 1992-02-19 원본미기재 개선된 프라이머 연장반응
DE3929030A1 (de) * 1989-09-01 1991-03-07 Boehringer Mannheim Gmbh Verfahren zur vermehrung von nukleinsaeuren

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US5001050A (en) 1991-03-19
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WO1991016446A1 (en) 1991-10-31
EP0527728B1 (de) 1997-07-09
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DE69031043T2 (de) 1997-10-30
JP2907231B2 (ja) 1999-06-21

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