CA2449190A1 - Use of optical diffraction elements in detection methods - Google Patents
Use of optical diffraction elements in detection methods Download PDFInfo
- Publication number
- CA2449190A1 CA2449190A1 CA002449190A CA2449190A CA2449190A1 CA 2449190 A1 CA2449190 A1 CA 2449190A1 CA 002449190 A CA002449190 A CA 002449190A CA 2449190 A CA2449190 A CA 2449190A CA 2449190 A1 CA2449190 A1 CA 2449190A1
- Authority
- CA
- Canada
- Prior art keywords
- optical
- sample
- carrier
- volume elements
- luminescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000003287 optical effect Effects 0.000 title claims abstract description 75
- 238000001514 detection method Methods 0.000 title claims description 31
- 238000000034 method Methods 0.000 claims abstract description 68
- 238000002060 fluorescence correlation spectroscopy Methods 0.000 claims abstract description 9
- 239000000523 sample Substances 0.000 claims description 45
- 239000002245 particle Substances 0.000 claims description 31
- 238000005259 measurement Methods 0.000 claims description 26
- 108020004707 nucleic acids Proteins 0.000 claims description 26
- 102000039446 nucleic acids Human genes 0.000 claims description 26
- 150000007523 nucleic acids Chemical class 0.000 claims description 26
- 239000012491 analyte Substances 0.000 claims description 19
- 230000005284 excitation Effects 0.000 claims description 14
- 238000004020 luminiscence type Methods 0.000 claims description 11
- 239000003153 chemical reaction reagent Substances 0.000 claims description 10
- 230000027455 binding Effects 0.000 claims description 9
- 239000011159 matrix material Substances 0.000 claims description 9
- 239000004033 plastic Substances 0.000 claims description 7
- 229920003023 plastic Polymers 0.000 claims description 7
- 238000012163 sequencing technique Methods 0.000 claims description 7
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicon dioxide Inorganic materials O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 5
- 229910052751 metal Inorganic materials 0.000 claims description 5
- 239000002184 metal Substances 0.000 claims description 5
- 238000007899 nucleic acid hybridization Methods 0.000 claims description 5
- 230000005855 radiation Effects 0.000 claims description 5
- 239000002131 composite material Substances 0.000 claims description 4
- 230000014509 gene expression Effects 0.000 claims description 4
- 238000004458 analytical method Methods 0.000 claims description 3
- 150000002739 metals Chemical class 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 230000003321 amplification Effects 0.000 claims description 2
- 238000006911 enzymatic reaction Methods 0.000 claims description 2
- 239000012634 fragment Substances 0.000 claims description 2
- 230000001678 irradiating effect Effects 0.000 claims description 2
- 238000002356 laser light scattering Methods 0.000 claims description 2
- 230000004001 molecular interaction Effects 0.000 claims description 2
- 230000035772 mutation Effects 0.000 claims description 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 2
- 239000010453 quartz Substances 0.000 claims description 2
- 230000000717 retained effect Effects 0.000 claims description 2
- 238000006243 chemical reaction Methods 0.000 claims 1
- 230000001419 dependent effect Effects 0.000 claims 1
- 238000010195 expression analysis Methods 0.000 claims 1
- 238000002844 melting Methods 0.000 claims 1
- 230000008018 melting Effects 0.000 claims 1
- 238000010791 quenching Methods 0.000 claims 1
- 230000000171 quenching effect Effects 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 238000002296 dynamic light scattering Methods 0.000 abstract description 2
- 230000000638 stimulation Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 9
- 239000002773 nucleotide Substances 0.000 description 8
- 230000036961 partial effect Effects 0.000 description 8
- 239000000969 carrier Substances 0.000 description 7
- 125000003729 nucleotide group Chemical group 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 230000003993 interaction Effects 0.000 description 4
- 102000004169 proteins and genes Human genes 0.000 description 4
- 239000013543 active substance Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 102000054765 polymorphisms of proteins Human genes 0.000 description 3
- 229910052710 silicon Inorganic materials 0.000 description 3
- 239000010703 silicon Substances 0.000 description 3
- 235000012239 silicon dioxide Nutrition 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 108091005461 Nucleic proteins Proteins 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 230000005684 electric field Effects 0.000 description 2
- 238000005530 etching Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000000377 silicon dioxide Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-M Acrylate Chemical compound [O-]C(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-M 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 238000000018 DNA microarray Methods 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 208000034826 Genetic Predisposition to Disease Diseases 0.000 description 1
- 241000124008 Mammalia Species 0.000 description 1
- CERQOIWHTDAKMF-UHFFFAOYSA-M Methacrylate Chemical compound CC(=C)C([O-])=O CERQOIWHTDAKMF-UHFFFAOYSA-M 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 229920001222 biopolymer Polymers 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000000875 corresponding effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000001746 injection moulding Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 229910044991 metal oxide Inorganic materials 0.000 description 1
- 150000004706 metal oxides Chemical class 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 150000004767 nitrides Chemical class 0.000 description 1
- 229920002120 photoresistant polymer Polymers 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 210000004215 spore Anatomy 0.000 description 1
- 238000013513 substance screening Methods 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6421—Measuring at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10126083.0 | 2001-05-29 | ||
| DE10126083A DE10126083A1 (de) | 2001-05-29 | 2001-05-29 | Verwendung von optischen Diffraktionselementen in Nachweisverfahren |
| PCT/EP2002/005866 WO2002097406A1 (de) | 2001-05-29 | 2002-05-28 | Verwendung von optischen diffraktionselementen in nachweisverfahren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2449190A1 true CA2449190A1 (en) | 2002-12-05 |
Family
ID=7686488
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002449190A Abandoned CA2449190A1 (en) | 2001-05-29 | 2002-05-28 | Use of optical diffraction elements in detection methods |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US7259847B2 (enExample) |
| EP (1) | EP1390725B1 (enExample) |
| JP (1) | JP2004530137A (enExample) |
| CA (1) | CA2449190A1 (enExample) |
| DE (1) | DE10126083A1 (enExample) |
| WO (1) | WO2002097406A1 (enExample) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1637871B1 (en) * | 2003-05-30 | 2011-03-23 | Olympus Corporation | Measuring apparatus including a light-receiving unit |
| DE10336080A1 (de) * | 2003-08-06 | 2005-03-03 | Gnothis Holding Sa | Verfahren und Vorrichtung zur Bestimmung von lumineszierenden Molekülen, insbesondere nach der Methode der Fluoreszenzkorrelationsspektroskopie |
| DE202004012992U1 (de) * | 2004-08-19 | 2005-12-29 | Storz Endoskop Produktions Gmbh | Endoskopisches Video-Meßsystem |
| JP2008523383A (ja) | 2004-12-10 | 2008-07-03 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | マルチスポット調査装置 |
| DE102005000840B4 (de) * | 2005-01-05 | 2007-12-27 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren und Vorrichtung zur Elementanalyse mittels Laser-Emissionsspektrometrie |
| CN100372947C (zh) * | 2005-03-03 | 2008-03-05 | 华南师范大学 | 转基因物种的荧光相关光谱检测方法 |
| US20100049055A1 (en) * | 2005-05-31 | 2010-02-25 | W.O.M. World Of Medicine Ag | Method and apparatus for visual characterization of tissue |
| US20100167413A1 (en) * | 2007-05-10 | 2010-07-01 | Paul Lundquist | Methods and systems for analyzing fluorescent materials with reduced autofluorescence |
| US20080277595A1 (en) * | 2007-05-10 | 2008-11-13 | Pacific Biosciences Of California, Inc. | Highly multiplexed confocal detection systems and methods of using same |
| AU2008251861B2 (en) * | 2007-05-10 | 2014-03-20 | Pacific Biosciences Of California, Inc. | Methods and systems for analyzing fluorescent materials with reduced autofluorescence |
| DE102010016818A1 (de) * | 2010-03-16 | 2011-09-22 | Leica Microsystems Cms Gmbh | Verfahren und Vorrichtung zur Durchführung von Multipoint-FCS |
| WO2013131888A1 (en) | 2012-03-06 | 2013-09-12 | Rudolf Rigler | Cyclic single molecule sequencing process |
| CN106460034B (zh) | 2014-01-10 | 2021-05-07 | 格诺特斯控股股份公司 | 具有高准确度的单分子分析 |
| EP3112841A1 (en) | 2015-06-30 | 2017-01-04 | Gnothis Holding SA | Single molecule analysis in an electrical field |
| EP3330763A1 (en) | 2016-12-05 | 2018-06-06 | Gnothis AB | Apparatus for characterizing luminescent entities |
| DE102017105113B4 (de) | 2017-03-10 | 2021-09-30 | Leibniz-Institut für Photonische Technologien e.V. (Engl.Leibniz Institute of Photonic Technology) | Anordnung und Verfahren für die Erfassung von Änderungen der optischen Weglänge in einem Nano-Kapillarkanal |
| CN112912715B (zh) * | 2018-10-25 | 2024-08-09 | 浜松光子学株式会社 | 光学测定装置及光学测定方法 |
| EP4379378A1 (en) | 2022-11-29 | 2024-06-05 | Gnothis Holding AG | Selective-area coated protein resistant surface |
| EP4616193A1 (en) | 2022-11-07 | 2025-09-17 | Gnothis Holding AG | Selective-area coated protein resistant surface |
| EP4623095A1 (en) | 2022-11-21 | 2025-10-01 | Gnothis Holding AG | Multiple molecular binding sites |
| EP4403643A1 (en) | 2023-01-19 | 2024-07-24 | Gnothis Holding AG | Multiple molecular binding sites |
| EP4375644A1 (en) | 2022-11-22 | 2024-05-29 | Gnothis Holding AG | Fingerprint analysis of single molecule events |
| EP4379355A1 (en) | 2022-11-29 | 2024-06-05 | Gnothis Holding AG | High throughput analysis of single molecule events |
| EP4382614A1 (en) | 2022-12-06 | 2024-06-12 | Gnothis Holding AG | High yield support |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0679251T3 (da) | 1993-01-18 | 1999-01-25 | Evotec Biosystems Aktiengesell | Fremgangsmåde og apparat til vurdering af biopolymerers fitness |
| JP3474652B2 (ja) * | 1994-11-11 | 2003-12-08 | 株式会社モリテックス | 多点レーザトラッピング装置及びその方法 |
| DE19748211A1 (de) | 1997-10-31 | 1999-05-06 | Zeiss Carl Fa | Optisches Array-System und Reader für Mikrotiterplatten |
| US6055106A (en) * | 1998-02-03 | 2000-04-25 | Arch Development Corporation | Apparatus for applying optical gradient forces |
| US6208411B1 (en) * | 1998-09-28 | 2001-03-27 | Kla-Tencor Corporation | Massively parallel inspection and imaging system |
| US6455861B1 (en) * | 1998-11-24 | 2002-09-24 | Cambridge Research & Instrumentation, Inc. | Fluorescence polarization assay system and method |
| GB9906929D0 (en) * | 1999-03-26 | 1999-05-19 | Univ Glasgow | Assay system |
| JP2001228756A (ja) * | 2000-02-18 | 2001-08-24 | Canon Inc | 画像形成装置 |
| WO2001063262A1 (en) * | 2000-02-25 | 2001-08-30 | Cambridge Research & Instrumentation Inc. | Multiple label fluorescence polarization assay system and method |
| DE10065632A1 (de) | 2000-05-12 | 2001-11-15 | Smtech Biovision Holding Ag Ec | Verfahren zum Nachweis von Polynukleotiden |
| DE10065626A1 (de) | 2000-06-30 | 2002-01-10 | Smtech Biovision Holding Ag Ec | Einzelmolekül-Sequenzierungsverfahren |
-
2001
- 2001-05-29 DE DE10126083A patent/DE10126083A1/de not_active Withdrawn
-
2002
- 2002-05-28 JP JP2003500537A patent/JP2004530137A/ja active Pending
- 2002-05-28 WO PCT/EP2002/005866 patent/WO2002097406A1/de not_active Ceased
- 2002-05-28 CA CA002449190A patent/CA2449190A1/en not_active Abandoned
- 2002-05-28 EP EP02747328.9A patent/EP1390725B1/de not_active Expired - Lifetime
- 2002-05-28 US US10/479,177 patent/US7259847B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE10126083A1 (de) | 2002-12-05 |
| WO2002097406A1 (de) | 2002-12-05 |
| EP1390725A1 (de) | 2004-02-25 |
| US20040126780A1 (en) | 2004-07-01 |
| JP2004530137A (ja) | 2004-09-30 |
| US7259847B2 (en) | 2007-08-21 |
| EP1390725B1 (de) | 2015-12-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7259847B2 (en) | Use of optical diffraction elements in detection methods | |
| AU698306B2 (en) | Chemical, biochemical and biological processing in thin films | |
| Dörre et al. | Techniques for single molecule sequencing | |
| US20120040364A1 (en) | Compensator for multiple surface imaging | |
| EP2765424A1 (en) | Method for analyzing biomolecules and biomolecule analyzer | |
| JP5127718B2 (ja) | 複雑な組成を有する生物学的起源のサンプル中の１種若しくはそれ以上の被検体の測定方法およびその使用 | |
| US11415515B2 (en) | Systems and methods for multicolor imaging | |
| JP2005536725A (ja) | マルチプレックス実験用の回折格子ベースのコード化マイクロパーティクル | |
| Rutten et al. | Boosting biomolecular interactions through DNA origami nano-tailored biosensing interfaces | |
| US7223985B2 (en) | Single-channel multicolored correlation analysis | |
| US20040151631A1 (en) | Determination of analytes by means of fluorescence correlation spectroscopy | |
| DE10210737A1 (de) | Einkanal-Mehrfarben-Korrelationsanalyse | |
| JP5133895B2 (ja) | 生体分子の親和性を測定する方法 | |
| US20090022204A1 (en) | Method for temperature measurement in a microfluid channel of a microfluid device | |
| KR100543705B1 (ko) | 2차원적 격자가 형성되어 있는 기판을 포함하는 마이크로 어레이 및 그를 이용하는 표적 분자의 검출방법 | |
| Hosoi et al. | Development of microreactor array chip‐based measurement system for massively parallel analysis of enzymatic activity | |
| CN120659891A (zh) | 单分子事件的高通量分析 | |
| CA2440183A1 (en) | Determination of analytes by means of fluorescence correlation spectroscopy | |
| JP2025540950A (ja) | 単一分子イベントのハイスループット解析 | |
| Lopez et al. | Laser wave-mixing optical method for sensitive detection of analytes in microarrays and microchips | |
| HK1154653B (en) | Compensator for multiple surface imaging | |
| Peham | Microdevices for molecular diagnostics: continuous flow PCR, nucleotide fluorescence labelling, DNA sensing and fluorescence enhancement | |
| HK1091904B (en) | Imaging fluorescence signals using telecentric optics | |
| HK1091904A1 (zh) | 使用远心光学器件成像荧光信号 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |