CA2437043C - Ligand detection method - Google Patents
Ligand detection method Download PDFInfo
- Publication number
- CA2437043C CA2437043C CA2437043A CA2437043A CA2437043C CA 2437043 C CA2437043 C CA 2437043C CA 2437043 A CA2437043 A CA 2437043A CA 2437043 A CA2437043 A CA 2437043A CA 2437043 C CA2437043 C CA 2437043C
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- sample
- receptors
- binding
- ligand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
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- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
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- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB0029617.8 | 2000-12-05 | ||
| GBGB0029617.8A GB0029617D0 (en) | 2000-12-05 | 2000-12-05 | Ligand detection method |
| PCT/GB2001/005388 WO2002046464A2 (en) | 2000-12-05 | 2001-12-05 | Ligand detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2437043A1 CA2437043A1 (en) | 2002-06-13 |
| CA2437043C true CA2437043C (en) | 2011-10-11 |
Family
ID=9904463
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2437043A Expired - Fee Related CA2437043C (en) | 2000-12-05 | 2001-12-05 | Ligand detection method |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US7910294B2 (cg-RX-API-DMAC10.html) |
| EP (1) | EP1364057B1 (cg-RX-API-DMAC10.html) |
| JP (1) | JP4266634B2 (cg-RX-API-DMAC10.html) |
| AR (1) | AR031631A1 (cg-RX-API-DMAC10.html) |
| AT (1) | ATE429510T1 (cg-RX-API-DMAC10.html) |
| AU (1) | AU2002220898A1 (cg-RX-API-DMAC10.html) |
| CA (1) | CA2437043C (cg-RX-API-DMAC10.html) |
| CY (1) | CY1109246T1 (cg-RX-API-DMAC10.html) |
| DE (1) | DE60138486D1 (cg-RX-API-DMAC10.html) |
| DK (1) | DK1364057T3 (cg-RX-API-DMAC10.html) |
| ES (1) | ES2324462T3 (cg-RX-API-DMAC10.html) |
| GB (2) | GB0029617D0 (cg-RX-API-DMAC10.html) |
| PT (1) | PT1364057E (cg-RX-API-DMAC10.html) |
| WO (1) | WO2002046464A2 (cg-RX-API-DMAC10.html) |
Families Citing this family (33)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69940647D1 (de) | 1998-05-01 | 2009-05-07 | Gen Probe Inc | Automatische diagnostische Analysevorrichtung |
| US8337753B2 (en) | 1998-05-01 | 2012-12-25 | Gen-Probe Incorporated | Temperature-controlled incubator having a receptacle mixing mechanism |
| EP1602733A1 (en) * | 2004-06-02 | 2005-12-07 | Keygene N.V. | Detection of target nucleotide sequences using an asymmetric oligonucleotide ligation assay |
| JP5117722B2 (ja) | 2003-09-02 | 2013-01-16 | キージーン ナムローゼ フェンノートシャップ | 標的核酸配列の検出のためのｏｌａベースの方法 |
| US20050214805A1 (en) * | 2003-11-10 | 2005-09-29 | Q-Rna, Inc. | Methods of detection employing immuno-Q-Amp technology |
| US20060199194A1 (en) * | 2003-11-10 | 2006-09-07 | Q-Rna, Inc. | Methods of detection using immuno-Q-Amp technology |
| WO2005054860A1 (en) * | 2003-12-01 | 2005-06-16 | Dako Denmark A/S | Methods and compositions for immuno-histochemical detection |
| EP1921454B1 (en) * | 2005-03-10 | 2015-08-12 | Gen-Probe Incorporated | Systems and methods to perform assays for detecting or quantifying analytes |
| US7794659B2 (en) * | 2005-03-10 | 2010-09-14 | Gen-Probe Incorporated | Signal measuring system having a movable signal measuring device |
| DE102008062372B3 (de) * | 2008-12-17 | 2010-06-17 | Medizinische Hochschule Hannover | Nachweiskonjugat und Verfahren zur Analyse |
| KR20130029128A (ko) | 2010-07-23 | 2013-03-21 | 베크만 컬터, 인코포레이티드 | 실시간 pcr용 용기 |
| US9046507B2 (en) | 2010-07-29 | 2015-06-02 | Gen-Probe Incorporated | Method, system and apparatus for incorporating capacitive proximity sensing in an automated fluid transfer procedure |
| EP2678664B1 (en) | 2011-02-24 | 2019-08-07 | Gen-Probe Incorporated | Systems and methods for distinguishing optical signals of different modulation frequencies in an optical signal detector |
| CN104040352B (zh) | 2011-11-07 | 2016-09-07 | 贝克曼考尔特公司 | 机械臂 |
| WO2013070755A2 (en) | 2011-11-07 | 2013-05-16 | Beckman Coulter, Inc. | Centrifuge system and workflow |
| EP3373015A1 (en) | 2011-11-07 | 2018-09-12 | Beckman Coulter Inc. | Aliquotter system and workflow |
| US8973736B2 (en) | 2011-11-07 | 2015-03-10 | Beckman Coulter, Inc. | Magnetic damping for specimen transport system |
| US9910054B2 (en) | 2011-11-07 | 2018-03-06 | Beckman Coulter, Inc. | System and method for processing samples |
| KR20140091033A (ko) | 2011-11-07 | 2014-07-18 | 베크만 컬터, 인코포레이티드 | 검체 컨테이너 검출 |
| US20140322706A1 (en) | 2012-10-24 | 2014-10-30 | Jon Faiz Kayyem | Integrated multipelx target analysis |
| US9957553B2 (en) | 2012-10-24 | 2018-05-01 | Genmark Diagnostics, Inc. | Integrated multiplex target analysis |
| CN107866286A (zh) | 2013-03-15 | 2018-04-03 | 金马克诊断股份有限公司 | 用于操纵可变形流体容器的系统、方法和设备 |
| CN107831324B (zh) | 2013-03-15 | 2021-11-19 | 雅培制药有限公司 | 具有后面可进入轨道系统的自动化诊断分析仪及相关方法 |
| CN105190317B (zh) | 2013-03-15 | 2018-05-04 | 雅培制药有限公司 | 具有预处理转盘的诊断分析机及相关方法 |
| CN105378487B (zh) | 2013-03-15 | 2018-08-21 | 雅培制药有限公司 | 具有竖直布置的圆盘传送带的自动化诊断分析仪及相关方法 |
| USD881409S1 (en) | 2013-10-24 | 2020-04-14 | Genmark Diagnostics, Inc. | Biochip cartridge |
| US9498778B2 (en) | 2014-11-11 | 2016-11-22 | Genmark Diagnostics, Inc. | Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system |
| US9598722B2 (en) | 2014-11-11 | 2017-03-21 | Genmark Diagnostics, Inc. | Cartridge for performing assays in a closed sample preparation and reaction system |
| US10005080B2 (en) | 2014-11-11 | 2018-06-26 | Genmark Diagnostics, Inc. | Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation |
| CN106932590A (zh) * | 2015-12-31 | 2017-07-07 | 复旦大学 | 一种定量检测低丰度蛋白及其后修饰蛋白的方法 |
| US10427162B2 (en) | 2016-12-21 | 2019-10-01 | Quandx Inc. | Systems and methods for molecular diagnostics |
| WO2019200326A1 (en) | 2018-04-13 | 2019-10-17 | Rarecyte, Inc. | Kits for labeling of biomarkers and methods of using the same |
| US20230138285A1 (en) * | 2021-11-04 | 2023-05-04 | Miltenyi Biotec B.V. & Co. KG | Bright and releasable labels for cell staining based on the conjugates with several sites of fluorophore release |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5656731A (en) * | 1987-10-15 | 1997-08-12 | Chiron Corporation | Nucleic acid-amplified immunoassay probes |
| US6083689A (en) * | 1990-10-16 | 2000-07-04 | Bayer Corporation | Sensitive immunoassays utilizing antibody conjugates with replicable DNA templates |
| WO1993001498A1 (en) * | 1991-07-04 | 1993-01-21 | Immunodex K/S | Water-soluble, polymer-based reagents and conjugates comprising moieties derived from divinyl sulfone |
| US5665539A (en) * | 1991-07-12 | 1997-09-09 | The Regents Of The University Of California | Immuno-polymerase chain reaction system for antigen detection |
| WO1994026932A1 (en) * | 1993-05-13 | 1994-11-24 | United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Nucleic acid tagged immunoassay |
| WO1995008626A1 (en) * | 1993-09-20 | 1995-03-30 | The Regents Of The University Of Colorado | Strategy for the production of rna from immobilized templates |
| JP2909216B2 (ja) * | 1994-04-29 | 1999-06-23 | パーキン‐エルマー コーポレイション | 核酸増幅生成物のリアルタイム検出装置 |
| US5854033A (en) * | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| DE69734576T2 (de) * | 1996-03-01 | 2006-08-03 | E.I. Dupont De Nemours And Co., Wilmington | Methode zur vervielfältigung und zum nachweis eines gewünschten nukleinsäurefragments |
| US6117631A (en) * | 1996-10-29 | 2000-09-12 | Polyprobe, Inc. | Detection of antigens via oligonucleotide antibody conjugates |
| WO1998020148A1 (en) * | 1996-11-04 | 1998-05-14 | The Regents Of The University Of California | Method for detection of pathogens in food |
| US6255060B1 (en) * | 1996-11-21 | 2001-07-03 | Trustees Of The University Of Pennsylvania | Method of detecting protein by immuno RNA |
| US5922553A (en) * | 1996-11-21 | 1999-07-13 | Trustees Of The University Of Pennsylvania | Method of detecting protein by immuno RNA |
| AT407160B (de) | 1998-06-04 | 2001-01-25 | Immuno Ag | Verfahren zur bestimmung von antigenen |
| DE19915141C2 (de) * | 1999-03-26 | 2002-11-21 | Artus Ges Fuer Molekularbiolog | Detektion von Nucleinsäure-Amplifikaten |
| EP1232283B1 (fr) | 1999-10-27 | 2006-05-24 | Université de Liège | Immuno PCR en temps réel utilisant un ADN-chimère comme marqueur d'amplification |
-
2000
- 2000-12-05 GB GBGB0029617.8A patent/GB0029617D0/en not_active Ceased
-
2001
- 2001-12-05 US US10/433,548 patent/US7910294B2/en not_active Expired - Fee Related
- 2001-12-05 ES ES01999671T patent/ES2324462T3/es not_active Expired - Lifetime
- 2001-12-05 CA CA2437043A patent/CA2437043C/en not_active Expired - Fee Related
- 2001-12-05 AT AT01999671T patent/ATE429510T1/de active
- 2001-12-05 DK DK01999671T patent/DK1364057T3/da active
- 2001-12-05 AR ARP010105648A patent/AR031631A1/es active IP Right Grant
- 2001-12-05 JP JP2002548181A patent/JP4266634B2/ja not_active Expired - Fee Related
- 2001-12-05 WO PCT/GB2001/005388 patent/WO2002046464A2/en not_active Ceased
- 2001-12-05 AU AU2002220898A patent/AU2002220898A1/en not_active Abandoned
- 2001-12-05 PT PT01999671T patent/PT1364057E/pt unknown
- 2001-12-05 DE DE60138486T patent/DE60138486D1/de not_active Expired - Lifetime
- 2001-12-05 EP EP01999671A patent/EP1364057B1/en not_active Expired - Lifetime
- 2001-12-05 GB GB0129143A patent/GB2375822B/en not_active Expired - Fee Related
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2009
- 2009-07-17 CY CY20091100763T patent/CY1109246T1/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| GB0029617D0 (en) | 2001-01-17 |
| ATE429510T1 (de) | 2009-05-15 |
| AR031631A1 (es) | 2003-09-24 |
| EP1364057B1 (en) | 2009-04-22 |
| WO2002046464A3 (en) | 2003-09-12 |
| GB0129143D0 (en) | 2002-01-23 |
| AU2002220898A1 (en) | 2002-06-18 |
| PT1364057E (pt) | 2009-07-06 |
| GB2375822A (en) | 2002-11-27 |
| CY1109246T1 (el) | 2014-07-02 |
| US20040076983A1 (en) | 2004-04-22 |
| CA2437043A1 (en) | 2002-06-13 |
| JP2004520574A (ja) | 2004-07-08 |
| WO2002046464A2 (en) | 2002-06-13 |
| DK1364057T3 (da) | 2009-08-03 |
| EP1364057A2 (en) | 2003-11-26 |
| GB2375822B (en) | 2005-04-06 |
| ES2324462T3 (es) | 2009-08-07 |
| US7910294B2 (en) | 2011-03-22 |
| JP4266634B2 (ja) | 2009-05-20 |
| DE60138486D1 (de) | 2009-06-04 |
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