CA2424779C - Processes for clonal growth of hepatic progenitor cells - Google Patents

Processes for clonal growth of hepatic progenitor cells Download PDF

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Publication number
CA2424779C
CA2424779C CA2424779A CA2424779A CA2424779C CA 2424779 C CA2424779 C CA 2424779C CA 2424779 A CA2424779 A CA 2424779A CA 2424779 A CA2424779 A CA 2424779A CA 2424779 C CA2424779 C CA 2424779C
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cells
hepatic
culture medium
progenitors
cell
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Expired - Fee Related
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CA2424779A
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English (en)
French (fr)
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CA2424779A1 (en
Inventor
Hiroshi Kubota
Lola M. Reid
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University of North Carolina at Chapel Hill
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University of North Carolina at Chapel Hill
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0607Non-embryonic pluripotent stem cells, e.g. MASC
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    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/067Hepatocytes
    • C12N5/0672Stem cells; Progenitor cells; Precursor cells; Oval cells
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    • C12N2500/00Specific components of cell culture medium
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    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/24Iron; Fe chelators; Transferrin
    • C12N2500/25Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2500/36Lipids
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    • C12N2500/38Vitamins
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    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10Growth factors
    • C12N2501/11Epidermal growth factor [EGF]
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30Hormones
    • C12N2501/33Insulin
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    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30Hormones
    • C12N2501/38Hormones with nuclear receptors
    • C12N2501/39Steroid hormones
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    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/13Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2424779A 2000-10-03 2000-10-03 Processes for clonal growth of hepatic progenitor cells Expired - Fee Related CA2424779C (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2000/027428 WO2002029012A1 (en) 2000-10-03 2000-10-03 Processes for clonal growth of hepatic progenitor cells

Publications (2)

Publication Number Publication Date
CA2424779A1 CA2424779A1 (en) 2002-04-11
CA2424779C true CA2424779C (en) 2016-08-23

Family

ID=21741846

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2424779A Expired - Fee Related CA2424779C (en) 2000-10-03 2000-10-03 Processes for clonal growth of hepatic progenitor cells

Country Status (9)

Country Link
EP (1) EP1325111A1 (OSRAM)
JP (1) JP2004510434A (OSRAM)
KR (1) KR100887623B1 (OSRAM)
CN (1) CN1461341A (OSRAM)
AU (2) AU2000277535B2 (OSRAM)
CA (1) CA2424779C (OSRAM)
HK (1) HK1054570A1 (OSRAM)
IL (1) IL155387A0 (OSRAM)
WO (1) WO2002029012A1 (OSRAM)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2344653A1 (en) 1998-09-29 2000-04-06 Gamida Cell Ltd. Methods of controlling proliferation and differentiation of stem and progenitor cells
US20020187133A1 (en) 1999-10-01 2002-12-12 Hiroshi Kubota Methods of isolating bipotent hepatic progenitor cells
KR100947911B1 (ko) 2001-03-27 2010-03-17 버텍스 파마슈티칼스 인코포레이티드 Hcv 감염에 유용한 조성물 및 방법
IL152904A0 (en) * 2002-01-24 2003-06-24 Gamida Cell Ltd Utilization of retinoid and vitamin d receptor antagonists for expansion of renewable stem cell populations
DK1490480T3 (en) 2002-03-15 2016-04-11 Univ North Carolina PRIMITIVE AND PROXIMAL Liver Stem Cells
SG141296A1 (en) 2002-05-17 2008-04-28 Sinai School Medicine Mesoderm and definitive endoderm cell populations
US20060003446A1 (en) 2002-05-17 2006-01-05 Gordon Keller Mesoderm and definitive endoderm cell populations
US8669109B2 (en) 2003-08-19 2014-03-11 Boehringer Ingelheim Pharma Gmbh & Co. Kg Methods of producing proteins in Chinese hamster ovary (CHO) cells
DE10338531A1 (de) 2003-08-19 2005-04-07 Boehringer Ingelheim Pharma Gmbh & Co. Kg Verfahren zur Reklonierung von Produktionszellen
US7332336B2 (en) 2003-08-19 2008-02-19 Effector Cell Institute, Inc. Methods for inducing differentiation of pluripotent cells
CA2553303C (en) * 2004-01-14 2014-06-10 Novahep Ab Human hepatic progenitor cells and methods of use thereof
US20080085554A1 (en) * 2004-02-13 2008-04-10 Norio Nakatsuji Culture Medium for Culturing Feeder Cells for Embryonic Stem Cells Culture and the Prepared Feeder Cells
US8874380B2 (en) 2010-12-09 2014-10-28 Rutgers, The State University Of New Jersey Method of overcoming therapeutic limitations of nonuniform distribution of radiopharmaceuticals and chemotherapy drugs
WO2008056368A2 (en) * 2006-11-09 2008-05-15 Gamida-Cell Ltd. Use of ex-vivo cultured hematopoietic cells for treatment of peripheral vascular diseases
CN101275121B (zh) * 2007-03-26 2011-05-11 芦银雪 体外培养扩增的人肝脏祖先细胞及其制备方法
CN102083967B (zh) * 2007-07-20 2013-05-15 塞拉提斯股份公司 经由定型内胚层(DE-hep)衍生自人胚泡衍生干细胞的新肝细胞群体
CN101701219B (zh) * 2009-11-06 2013-02-20 哈尔滨医科大学 编码兔肝细胞生长因子的cDNA及其表达载体和应用
JP6232638B2 (ja) * 2013-06-24 2017-11-22 国立研究開発法人医薬基盤・健康・栄養研究所 肝前駆細胞増殖用培地
CN104630130A (zh) * 2014-12-05 2015-05-20 新乡医学院 一种大鼠肝细胞分离方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4914032A (en) * 1985-06-06 1990-04-03 Centro De Investigation Y Estudios Avanzados Del Instituto Politecnico Nacional Process for the long-term surviving culture of hepatocytes
AU667680B2 (en) * 1991-08-07 1996-04-04 Albert Einstein College Of Medicine Of Yeshiva University Proliferation of hepatocyte precursors
ATE318075T1 (de) * 1993-11-19 2006-03-15 Einstein Coll Med Hepatoblasten und verfahren zu ihrer isolierung
CA2146735C (en) * 1994-04-11 2007-02-20 Chise Tateno Liver parenchymal cells having clonal growth ability, method for obtaining same, method for subculturing same, and subculturing system of primary hepatocytes
JP3266766B2 (ja) * 1994-08-23 2002-03-18 科学技術振興事業団 ローン性増殖能を有する肝実質細胞とその取得方法、並びにその継代培養方法
KR100873690B1 (ko) * 1999-01-19 2008-12-12 유니버시티 오브 노스캐롤라이나 앳 채플 힐 인간 간 전구세포
JP2004510432A (ja) * 2000-10-03 2004-04-08 ユニバーシティ オブ ノース カロライナ 両能性肝前駆細胞の単離方法

Also Published As

Publication number Publication date
AU2000277535B2 (en) 2007-03-29
WO2002029012A1 (en) 2002-04-11
IL155387A0 (en) 2003-11-23
AU7753500A (en) 2002-04-15
JP2004510434A (ja) 2004-04-08
CN1461341A (zh) 2003-12-10
KR20040047732A (ko) 2004-06-05
EP1325111A1 (en) 2003-07-09
KR100887623B1 (ko) 2009-03-11
CA2424779A1 (en) 2002-04-11
HK1054570A1 (zh) 2003-12-05

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