AU2000277535B2 - Processes for clonal growth of hepatic progenitor cells - Google Patents

Processes for clonal growth of hepatic progenitor cells Download PDF

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Publication number
AU2000277535B2
AU2000277535B2 AU2000277535A AU2000277535A AU2000277535B2 AU 2000277535 B2 AU2000277535 B2 AU 2000277535B2 AU 2000277535 A AU2000277535 A AU 2000277535A AU 2000277535 A AU2000277535 A AU 2000277535A AU 2000277535 B2 AU2000277535 B2 AU 2000277535B2
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cells
hepatic
progenitors
progeny
culture medium
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Ceased
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AU2000277535A
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AU2000277535A1 (en
Inventor
Hiroshi Kubota
Lola M Reid
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University of North Carolina at Chapel Hill
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University of North Carolina at Chapel Hill
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Assigned to UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL reassignment UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL Amend patent request/document other than specification (104) Assignors: UNIVERSITY OF NORTH CAROLINA
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Publication of AU2000277535B2 publication Critical patent/AU2000277535B2/en
Priority to AU2007202994A priority Critical patent/AU2007202994A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2000277535A 2000-10-03 2000-10-03 Processes for clonal growth of hepatic progenitor cells Ceased AU2000277535B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2007202994A AU2007202994A1 (en) 2000-10-03 2007-06-27 Processes for clonal growth of hepatic progenitor cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2000/027428 WO2002029012A1 (en) 2000-10-03 2000-10-03 Processes for clonal growth of hepatic progenitor cells

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2007202994A Division AU2007202994A1 (en) 2000-10-03 2007-06-27 Processes for clonal growth of hepatic progenitor cells

Publications (2)

Publication Number Publication Date
AU2000277535A1 AU2000277535A1 (en) 2002-06-27
AU2000277535B2 true AU2000277535B2 (en) 2007-03-29

Family

ID=21741846

Family Applications (2)

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AU2000277535A Ceased AU2000277535B2 (en) 2000-10-03 2000-10-03 Processes for clonal growth of hepatic progenitor cells
AU7753500A Pending AU7753500A (en) 2000-10-03 2000-10-03 Processes for clonal growth of hepatic progenitor cells

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU7753500A Pending AU7753500A (en) 2000-10-03 2000-10-03 Processes for clonal growth of hepatic progenitor cells

Country Status (9)

Country Link
EP (1) EP1325111A1 (OSRAM)
JP (1) JP2004510434A (OSRAM)
KR (1) KR100887623B1 (OSRAM)
CN (1) CN1461341A (OSRAM)
AU (2) AU2000277535B2 (OSRAM)
CA (1) CA2424779C (OSRAM)
HK (1) HK1054570A1 (OSRAM)
IL (1) IL155387A0 (OSRAM)
WO (1) WO2002029012A1 (OSRAM)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003233399B2 (en) * 2002-03-15 2008-03-20 University Of North Carolina At Chapel Hill Primitive and proximal hepatic stem cells
AU2003208577B2 (en) * 2002-01-24 2008-07-10 Gamida-Cell Ltd. Expansion of renewable stem cell populations
US8202724B2 (en) 1998-02-17 2012-06-19 Gamida Cell Ltd. Methods of controlling proliferation and differentiation of stem and progenitor cells

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020187133A1 (en) 1999-10-01 2002-12-12 Hiroshi Kubota Methods of isolating bipotent hepatic progenitor cells
KR100947911B1 (ko) 2001-03-27 2010-03-17 버텍스 파마슈티칼스 인코포레이티드 Ｈｃｖ 감염에 유용한 조성물 및 방법
SG141296A1 (en) 2002-05-17 2008-04-28 Sinai School Medicine Mesoderm and definitive endoderm cell populations
US20060003446A1 (en) 2002-05-17 2006-01-05 Gordon Keller Mesoderm and definitive endoderm cell populations
US8669109B2 (en) 2003-08-19 2014-03-11 Boehringer Ingelheim Pharma Gmbh & Co. Kg Methods of producing proteins in Chinese hamster ovary (CHO) cells
DE10338531A1 (de) 2003-08-19 2005-04-07 Boehringer Ingelheim Pharma Gmbh & Co. Kg Verfahren zur Reklonierung von Produktionszellen
US7332336B2 (en) 2003-08-19 2008-02-19 Effector Cell Institute, Inc. Methods for inducing differentiation of pluripotent cells
CA2553303C (en) * 2004-01-14 2014-06-10 Novahep Ab Human hepatic progenitor cells and methods of use thereof
US20080085554A1 (en) * 2004-02-13 2008-04-10 Norio Nakatsuji Culture Medium for Culturing Feeder Cells for Embryonic Stem Cells Culture and the Prepared Feeder Cells
US8874380B2 (en) 2010-12-09 2014-10-28 Rutgers, The State University Of New Jersey Method of overcoming therapeutic limitations of nonuniform distribution of radiopharmaceuticals and chemotherapy drugs
WO2008056368A2 (en) * 2006-11-09 2008-05-15 Gamida-Cell Ltd. Use of ex-vivo cultured hematopoietic cells for treatment of peripheral vascular diseases
CN101275121B (zh) * 2007-03-26 2011-05-11 芦银雪 体外培养扩增的人肝脏祖先细胞及其制备方法
CN102083967B (zh) * 2007-07-20 2013-05-15 塞拉提斯股份公司 经由定型内胚层(DE-hep)衍生自人胚泡衍生干细胞的新肝细胞群体
CN101701219B (zh) * 2009-11-06 2013-02-20 哈尔滨医科大学 编码兔肝细胞生长因子的cDNA及其表达载体和应用
JP6232638B2 (ja) * 2013-06-24 2017-11-22 国立研究開発法人医薬基盤・健康・栄養研究所 肝前駆細胞増殖用培地
CN104630130A (zh) * 2014-12-05 2015-05-20 新乡医学院 一种大鼠肝细胞分离方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993003142A1 (en) * 1991-08-07 1993-02-18 Albert Einstein College Of Medicine Proliferation of hepatocyte precursors

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4914032A (en) * 1985-06-06 1990-04-03 Centro De Investigation Y Estudios Avanzados Del Instituto Politecnico Nacional Process for the long-term surviving culture of hepatocytes
ATE318075T1 (de) * 1993-11-19 2006-03-15 Einstein Coll Med Hepatoblasten und verfahren zu ihrer isolierung
CA2146735C (en) * 1994-04-11 2007-02-20 Chise Tateno Liver parenchymal cells having clonal growth ability, method for obtaining same, method for subculturing same, and subculturing system of primary hepatocytes
JP3266766B2 (ja) * 1994-08-23 2002-03-18 科学技術振興事業団 ローン性増殖能を有する肝実質細胞とその取得方法、並びにその継代培養方法
KR100873690B1 (ko) * 1999-01-19 2008-12-12 유니버시티 오브 노스캐롤라이나 앳 채플 힐 인간 간 전구세포
JP2004510432A (ja) * 2000-10-03 2004-04-08 ユニバーシティ　オブ　ノース　カロライナ 両能性肝前駆細胞の単離方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993003142A1 (en) * 1991-08-07 1993-02-18 Albert Einstein College Of Medicine Proliferation of hepatocyte precursors

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Rogler, L.E (Feb. 1997) Am J Path, Vol. 150(2), 591-602 *

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8202724B2 (en) 1998-02-17 2012-06-19 Gamida Cell Ltd. Methods of controlling proliferation and differentiation of stem and progenitor cells
AU2003208577B2 (en) * 2002-01-24 2008-07-10 Gamida-Cell Ltd. Expansion of renewable stem cell populations
AU2003208577B9 (en) * 2002-01-24 2008-07-31 Gamida-Cell Ltd. Expansion of renewable stem cell populations
US7955852B2 (en) 2002-01-24 2011-06-07 Gamida Cell Ltd. Expansion of renewable stem cell populations
AU2003233399B2 (en) * 2002-03-15 2008-03-20 University Of North Carolina At Chapel Hill Primitive and proximal hepatic stem cells
AU2008202656B2 (en) * 2002-03-15 2011-03-17 University Of North Carolina At Chapel Hill Primitive and proximal hepatic stem cells
AU2008202656B8 (en) * 2002-03-15 2011-12-15 University Of North Carolina At Chapel Hill Primitive and proximal hepatic stem cells
AU2008202656A8 (en) * 2002-03-15 2011-12-15 University Of North Carolina At Chapel Hill Primitive and proximal hepatic stem cells

Also Published As

Publication number Publication date
WO2002029012A1 (en) 2002-04-11
IL155387A0 (en) 2003-11-23
AU7753500A (en) 2002-04-15
JP2004510434A (ja) 2004-04-08
CA2424779C (en) 2016-08-23
CN1461341A (zh) 2003-12-10
KR20040047732A (ko) 2004-06-05
EP1325111A1 (en) 2003-07-09
KR100887623B1 (ko) 2009-03-11
CA2424779A1 (en) 2002-04-11
HK1054570A1 (zh) 2003-12-05

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DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE NAME OF THE APPLICANT/PATENTEE FROM UNIVERSITY OF NORTH CAROLINA TO UNIVERSITY OF NORTH CAROLINA AT CHAPEL HILL

FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired