CA2424779C - Procede de multiplication par clonage de cellules souches hepathiques - Google Patents

Procede de multiplication par clonage de cellules souches hepathiques Download PDF

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CA2424779C
CA2424779C CA2424779A CA2424779A CA2424779C CA 2424779 C CA2424779 C CA 2424779C CA 2424779 A CA2424779 A CA 2424779A CA 2424779 A CA2424779 A CA 2424779A CA 2424779 C CA2424779 C CA 2424779C
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cells
hepatic
culture medium
progenitors
cell
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Expired - Fee Related
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CA2424779A
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CA2424779A1 (fr
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Hiroshi Kubota
Lola M. Reid
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University of North Carolina at Chapel Hill
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University of North Carolina at Chapel Hill
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C12N5/0672—Stem cells; Progenitor cells; Precursor cells; Oval cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/33—Insulin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé permettant de propager des progéniteurs mammaliens d'origine endodermique, tels que les progéniteurs hépatiques, leur descendance, ou des mélanges de ceux-ci sur une couche de cellules nourricières embryonnaires mammaliennes dans un milieu de culture. Le milieu de culture peut être complété par une ou plusieurs hormones et par d'autres agents de croissance. Ces hormones et autres agents de croissance peuvent comprendre l'insuline, la dexaméthasone, la transferrine, la nicotinamide, la sérumalbumine, le .beta.-mercaptoéthanol, l'acide gras libre, la glutamine, le CuSO¿4? et le H¿2?SeO¿3?. le milieu de culture peut également comprendre des anticorps. Le milieu de culture ne comprend pas de sérum. Le procédé décrit dans cette invention comprend des moyens permettant d'inciter la différentiation des progéniteurs de leurs formes adultes, telle que la différentiation des cellules souches hépatiques des hépatocytes ou des cellules biliaires, par ajout ou par exclusion du facteur de croissance épidermique, respectivement. Le procédé de production de progéniteurs mammaliens est utile en ce que les progéniteurs peuvent être utilisés ultérieurement dans un ou plusieurs des procédés suivants: l'identification des facteurs de croissance et de différentiation, les études toxicologiques, la mise au point de médicaments, les études antimicrobiennes, ou la préparation d'un organe extra-corporel, tel qu'un foie bioartificiel.
CA2424779A 2000-10-03 2000-10-03 Procede de multiplication par clonage de cellules souches hepathiques Expired - Fee Related CA2424779C (fr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2000/027428 WO2002029012A1 (fr) 2000-10-03 2000-10-03 Procede de multiplication par clonage de cellules souches hepathiques

Publications (2)

Publication Number Publication Date
CA2424779A1 CA2424779A1 (fr) 2002-04-11
CA2424779C true CA2424779C (fr) 2016-08-23

Family

ID=21741846

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2424779A Expired - Fee Related CA2424779C (fr) 2000-10-03 2000-10-03 Procede de multiplication par clonage de cellules souches hepathiques

Country Status (9)

Country Link
EP (1) EP1325111A1 (fr)
JP (1) JP2004510434A (fr)
KR (1) KR100887623B1 (fr)
CN (1) CN1461341A (fr)
AU (2) AU7753500A (fr)
CA (1) CA2424779C (fr)
HK (1) HK1054570A1 (fr)
IL (1) IL155387A0 (fr)
WO (1) WO2002029012A1 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2344653A1 (fr) 1998-09-29 2000-04-06 Gamida Cell Ltd. Procedes de regulation de la proliferation et de la differentiation de cellules souches et precurseurs
US20020187133A1 (en) 1999-10-01 2002-12-12 Hiroshi Kubota Methods of isolating bipotent hepatic progenitor cells
ES2328466T3 (es) 2001-03-27 2009-11-13 Vertex Pharmaceuticals Incorporated Composiciones y metodos utiles para la infeccion por hcv.
IL152904A0 (en) * 2002-01-24 2003-06-24 Gamida Cell Ltd Utilization of retinoid and vitamin d receptor antagonists for expansion of renewable stem cell populations
HUE032238T2 (en) * 2002-03-15 2017-09-28 Univ North Carolina Chapel Hill Primitive and proximal liver stem cells
US20060003446A1 (en) 2002-05-17 2006-01-05 Gordon Keller Mesoderm and definitive endoderm cell populations
JP2006513727A (ja) 2002-05-17 2006-04-27 マウント シナイ スクール オブ メディスン オブ ニューヨーク ユニバーシティー 中胚葉および成体型内胚葉細胞集団
DE10338531A1 (de) * 2003-08-19 2005-04-07 Boehringer Ingelheim Pharma Gmbh & Co. Kg Verfahren zur Reklonierung von Produktionszellen
US7332336B2 (en) 2003-08-19 2008-02-19 Effector Cell Institute, Inc. Methods for inducing differentiation of pluripotent cells
US8669109B2 (en) 2003-08-19 2014-03-11 Boehringer Ingelheim Pharma Gmbh & Co. Kg Methods of producing proteins in Chinese hamster ovary (CHO) cells
JP5588587B2 (ja) * 2004-01-14 2014-09-10 ノーバヘップ アーベー ヒトの肝臓前駆細胞およびその使用方法
CA2555021A1 (fr) * 2004-02-13 2005-08-25 Reprocell, Inc. Moyen de preparer des cellules nourricieres pour des cellules souches embryonnaires et des cellules nourricieres
US8874380B2 (en) 2010-12-09 2014-10-28 Rutgers, The State University Of New Jersey Method of overcoming therapeutic limitations of nonuniform distribution of radiopharmaceuticals and chemotherapy drugs
US20080199441A1 (en) * 2006-11-09 2008-08-21 Tony Peled Use of ex-vivo cultured hematopoietic cells for treatment of peripheral vascular diseases
CN101275121B (zh) * 2007-03-26 2011-05-11 芦银雪 体外培养扩增的人肝脏祖先细胞及其制备方法
CN102083967B (zh) * 2007-07-20 2013-05-15 塞拉提斯股份公司 经由定型内胚层(DE-hep)衍生自人胚泡衍生干细胞的新肝细胞群体
CN101701219B (zh) * 2009-11-06 2013-02-20 哈尔滨医科大学 编码兔肝细胞生长因子的cDNA及其表达载体和应用
JP6232638B2 (ja) * 2013-06-24 2017-11-22 国立研究開発法人医薬基盤・健康・栄養研究所 肝前駆細胞増殖用培地
CN104630130A (zh) * 2014-12-05 2015-05-20 新乡医学院 一种大鼠肝细胞分离方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4914032A (en) * 1985-06-06 1990-04-03 Centro De Investigation Y Estudios Avanzados Del Instituto Politecnico Nacional Process for the long-term surviving culture of hepatocytes
EP1559778A1 (fr) * 1991-08-07 2005-08-03 Albert Einstein College Of Medicine Of Yeshiva University Prolifération de précurseurs d'hépatocytes
ATE318075T1 (de) * 1993-11-19 2006-03-15 Einstein Coll Med Hepatoblasten und verfahren zu ihrer isolierung
CA2146735C (fr) * 1994-04-11 2007-02-20 Chise Tateno Cellules parenchymateuses du foie capables de croissance clonale, methode pour les obtenir et pour en faire des sous-cultures, et systeme de sous-culture d'hepatocytes primaires
JP3266766B2 (ja) * 1994-08-23 2002-03-18 科学技術振興事業団 ローン性増殖能を有する肝実質細胞とその取得方法、並びにその継代培養方法
DK1147179T3 (da) * 1999-01-19 2009-03-09 Univ North Carolina Humane lever-progenitorer
CN1258589C (zh) * 2000-10-03 2006-06-07 北卡罗来纳大学 分离双能肝祖细胞的方法

Also Published As

Publication number Publication date
AU2000277535B2 (en) 2007-03-29
KR20040047732A (ko) 2004-06-05
JP2004510434A (ja) 2004-04-08
WO2002029012A1 (fr) 2002-04-11
HK1054570A1 (zh) 2003-12-05
AU7753500A (en) 2002-04-15
IL155387A0 (en) 2003-11-23
CN1461341A (zh) 2003-12-10
KR100887623B1 (ko) 2009-03-11
EP1325111A1 (fr) 2003-07-09
CA2424779A1 (fr) 2002-04-11

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