CA2423822A1 - Method for immobilising lipid layers - Google Patents
Method for immobilising lipid layers Download PDFInfo
- Publication number
- CA2423822A1 CA2423822A1 CA002423822A CA2423822A CA2423822A1 CA 2423822 A1 CA2423822 A1 CA 2423822A1 CA 002423822 A CA002423822 A CA 002423822A CA 2423822 A CA2423822 A CA 2423822A CA 2423822 A1 CA2423822 A1 CA 2423822A1
- Authority
- CA
- Canada
- Prior art keywords
- solid
- lipid
- body surface
- lipid layers
- molecules
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 150000002632 lipids Chemical class 0.000 title claims abstract description 143
- 238000000034 method Methods 0.000 title claims abstract description 69
- 239000007787 solid Substances 0.000 claims abstract description 37
- 239000012528 membrane Substances 0.000 claims description 50
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims description 45
- 239000011970 polystyrene sulfonate Substances 0.000 claims description 33
- 229960002796 polystyrene sulfonate Drugs 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 27
- 229920000642 polymer Polymers 0.000 claims description 26
- 102000004169 proteins and genes Human genes 0.000 claims description 25
- 108090000623 proteins and genes Proteins 0.000 claims description 25
- 229920002873 Polyethylenimine Polymers 0.000 claims description 19
- -1 alkyl disulfides Chemical class 0.000 claims description 16
- 239000006185 dispersion Substances 0.000 claims description 14
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- 239000000126 substance Substances 0.000 claims description 12
- 238000012986 modification Methods 0.000 claims description 11
- 230000004048 modification Effects 0.000 claims description 11
- 239000002245 particle Substances 0.000 claims description 11
- 230000003100 immobilizing effect Effects 0.000 claims description 10
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- 108090000790 Enzymes Proteins 0.000 claims description 9
- 238000007306 functionalization reaction Methods 0.000 claims description 8
- 229920001467 poly(styrenesulfonates) Polymers 0.000 claims description 8
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- 239000003446 ligand Substances 0.000 claims description 3
- PHQOGHDTIVQXHL-UHFFFAOYSA-N n'-(3-trimethoxysilylpropyl)ethane-1,2-diamine Chemical compound CO[Si](OC)(OC)CCCNCCN PHQOGHDTIVQXHL-UHFFFAOYSA-N 0.000 claims description 3
- 239000010970 precious metal Substances 0.000 claims description 3
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- 125000000524 functional group Chemical group 0.000 description 9
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Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/5436—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand physically entrapped within the solid phase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- General Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Medicinal Preparation (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE10048822A DE10048822A1 (de) | 2000-09-29 | 2000-09-29 | Verfahren zur Immobilisierung von Lipidschichten |
| DE10048822.6 | 2000-09-29 | ||
| PCT/EP2001/011036 WO2002027320A2 (de) | 2000-09-29 | 2001-09-25 | Verfahren zur immobilisierung von lipidschichten |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2423822A1 true CA2423822A1 (en) | 2003-03-27 |
Family
ID=7658458
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002423822A Abandoned CA2423822A1 (en) | 2000-09-29 | 2001-09-25 | Method for immobilising lipid layers |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20040023301A1 (xx) |
| EP (1) | EP1325334B1 (xx) |
| AT (1) | ATE535810T1 (xx) |
| AU (1) | AU1227802A (xx) |
| CA (1) | CA2423822A1 (xx) |
| DE (1) | DE10048822A1 (xx) |
| WO (1) | WO2002027320A2 (xx) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10121903A1 (de) * | 2001-04-27 | 2002-10-31 | Nimbus Biotechnologie Gmbh | System für die Membranpermeationsmessung |
| DE10125713A1 (de) * | 2001-05-21 | 2002-11-28 | Nimbus Biotechnologie Gmbh | Immobilisierte asymmetrische Lipiddoppelschichten |
| US7471846B2 (en) * | 2003-06-26 | 2008-12-30 | Fotonation Vision Limited | Perfecting the effect of flash within an image acquisition devices using face detection |
| DE102004038873A1 (de) * | 2004-08-05 | 2006-02-23 | Nimbus Biotechnologie Gmbh | Bestimmung von Bindungsparametern |
| AT502713B1 (de) * | 2005-10-19 | 2008-08-15 | Univ Wien Bodenkultur | Verfahren zur herstellung von lipid-membranen |
| JP4959710B2 (ja) * | 2005-11-01 | 2012-06-27 | レンセレアー ポリテクニック インスティテュート | 毒物学アッセイのための３次元細胞アレイチップおよびプラットフォーム |
| ITMI20052187A1 (it) * | 2005-11-15 | 2007-05-16 | Sunbird Investiments Ltd | Dispositivo di controllo della portata di un fluido |
| JP5167696B2 (ja) * | 2006-06-05 | 2013-03-21 | セントラル硝子株式会社 | フッ素化ナノダイヤモンド分散液の作製方法 |
| DE102007055865B3 (de) * | 2007-12-19 | 2009-10-01 | Leibniz-Institut Für Polymerforschung Dresden E.V. | Modifizierte Multi-Well-Platte für biochemische Analysen und Zellkulturexperimente |
| DE102008007673A1 (de) * | 2008-01-25 | 2009-07-30 | Nimbus Biotechnologie Gmbh | Bestimmung der Permeabilität von Substanzen durch die Blut-Hirn-Schranke |
| DE102010018965A1 (de) | 2010-04-27 | 2011-10-27 | Sovicell Gmbh | Bestimmung von Wechselwirkungen zwischen einer Substanz oder einem Substanzgemisch und einem Target |
| CN105241939B (zh) * | 2015-09-16 | 2017-08-01 | 山东理工大学 | 一种基于金银核壳磁性石墨烯吸附镉离子免疫传感器的制备方法及应用 |
| WO2023172993A1 (en) * | 2022-03-10 | 2023-09-14 | Margossian Khatcher Orbeli | Polyzwitterionic complexes and methods of using the same |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE4128953A1 (de) * | 1991-08-30 | 1993-03-04 | Basf Ag | Verfahren zur kultivierung von saeugerzellen im fliessbettreaktor |
| WO1996038726A1 (en) * | 1995-05-30 | 1996-12-05 | Ecole Polytechnique Federale De Lausanne (Epfl) | Covalently immobilized phospholipid bilayers on solid surfaces |
| DE19607279A1 (de) * | 1996-02-27 | 1997-08-28 | Bayer Ag | Durch Festkörper unterstützte Membran-Biosensoren |
| FR2751989B1 (fr) * | 1996-08-01 | 1998-10-30 | Soc D Rech Et De Dev En Activa | Methode d'identification de cellules eucaryotes par fixation desdites cellules sur un support et support pour mettre en oeuvre ladite methode |
| JPH11196865A (ja) * | 1998-01-19 | 1999-07-27 | Kansai Kagaku Kikai Seisaku Kk | 凝集性または接着性細胞の固定化およびその利用 |
| DE19814775C2 (de) * | 1998-04-02 | 2001-04-19 | Nimbus Biotechnologie Gmbh | Verfahren zur Bestimmung der Lipid-Bindungskonstanten von Substanzen in wässriger Lösung an Oberflächen aus amphiphilen Molekülen |
-
2000
- 2000-09-29 DE DE10048822A patent/DE10048822A1/de not_active Withdrawn
-
2001
- 2001-09-25 WO PCT/EP2001/011036 patent/WO2002027320A2/de active IP Right Grant
- 2001-09-25 US US10/381,851 patent/US20040023301A1/en not_active Abandoned
- 2001-09-25 AT AT01980432T patent/ATE535810T1/de active
- 2001-09-25 AU AU1227802A patent/AU1227802A/xx not_active Withdrawn
- 2001-09-25 CA CA002423822A patent/CA2423822A1/en not_active Abandoned
- 2001-09-25 EP EP01980432A patent/EP1325334B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO2002027320A2 (de) | 2002-04-04 |
| AU1227802A (en) | 2002-04-08 |
| ATE535810T1 (de) | 2011-12-15 |
| EP1325334A2 (de) | 2003-07-09 |
| EP1325334B1 (de) | 2011-11-30 |
| US20040023301A1 (en) | 2004-02-05 |
| WO2002027320A3 (de) | 2002-12-27 |
| DE10048822A1 (de) | 2002-04-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| FZDE | Discontinued |