CA2422440C - Oligonucleotide sequence formula for labeling oligonucleotide probes and proteins for in situ analysis - Google Patents
Oligonucleotide sequence formula for labeling oligonucleotide probes and proteins for in situ analysis Download PDFInfo
- Publication number
- CA2422440C CA2422440C CA2422440A CA2422440A CA2422440C CA 2422440 C CA2422440 C CA 2422440C CA 2422440 A CA2422440 A CA 2422440A CA 2422440 A CA2422440 A CA 2422440A CA 2422440 C CA2422440 C CA 2422440C
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- Prior art keywords
- probe
- oligonucleotide probe
- seq
- dna
- artificial sequence
- Prior art date
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- Expired - Lifetime
Links
- 108020005187 Oligonucleotide Probes Proteins 0.000 title claims abstract description 175
- 239000002751 oligonucleotide probe Substances 0.000 title claims abstract description 175
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 84
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- 102000004169 proteins and genes Human genes 0.000 title abstract description 7
- 108091034117 Oligonucleotide Proteins 0.000 title description 14
- 238000010249 in-situ analysis Methods 0.000 title description 3
- 239000000523 sample Substances 0.000 claims abstract description 181
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- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 21
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- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 3
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- QRXMUCSWCMTJGU-UHFFFAOYSA-L (5-bromo-4-chloro-1h-indol-3-yl) phosphate Chemical compound C1=C(Br)C(Cl)=C2C(OP([O-])(=O)[O-])=CNC2=C1 QRXMUCSWCMTJGU-UHFFFAOYSA-L 0.000 description 1
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- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- MJNIWUJSIGSWKK-UHFFFAOYSA-N Riboflavine 2',3',4',5'-tetrabutanoate Chemical compound CCCC(=O)OCC(OC(=O)CCC)C(OC(=O)CCC)C(OC(=O)CCC)CN1C2=CC(C)=C(C)C=C2N=C2C1=NC(=O)NC2=O MJNIWUJSIGSWKK-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2703141A CA2703141C (en) | 2000-09-15 | 2001-09-06 | Oligonucleotide sequence formula for labeling oligonucleotide probes and proteins for in situ analysis |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23317700P | 2000-09-15 | 2000-09-15 | |
| US60/233,177 | 2000-09-15 | ||
| PCT/US2001/028014 WO2002022874A2 (en) | 2000-09-15 | 2001-09-06 | Oligonucleotides for labeling oligonucleotide probes and proteins |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2703141A Division CA2703141C (en) | 2000-09-15 | 2001-09-06 | Oligonucleotide sequence formula for labeling oligonucleotide probes and proteins for in situ analysis |
Publications (2)
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|---|---|
| CA2422440A1 CA2422440A1 (en) | 2002-03-21 |
| CA2422440C true CA2422440C (en) | 2010-07-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CA2422440A Expired - Lifetime CA2422440C (en) | 2000-09-15 | 2001-09-06 | Oligonucleotide sequence formula for labeling oligonucleotide probes and proteins for in situ analysis |
| CA2703141A Expired - Lifetime CA2703141C (en) | 2000-09-15 | 2001-09-06 | Oligonucleotide sequence formula for labeling oligonucleotide probes and proteins for in situ analysis |
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| CA2703141A Expired - Lifetime CA2703141C (en) | 2000-09-15 | 2001-09-06 | Oligonucleotide sequence formula for labeling oligonucleotide probes and proteins for in situ analysis |
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| EP (1) | EP1366187B1 (enExample) |
| JP (2) | JP4986367B2 (enExample) |
| AT (1) | ATE415491T1 (enExample) |
| AU (2) | AU9454201A (enExample) |
| CA (2) | CA2422440C (enExample) |
| DE (1) | DE60136746D1 (enExample) |
| DK (1) | DK1366187T3 (enExample) |
| ES (1) | ES2317939T3 (enExample) |
| WO (1) | WO2002022874A2 (enExample) |
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| WO2002022874A2 (en) * | 2000-09-15 | 2002-03-21 | Ventana Medical Systems, Inc. | Oligonucleotides for labeling oligonucleotide probes and proteins |
| EP1399592A1 (en) * | 2001-06-06 | 2004-03-24 | Canag Diagnostics AB | Method to measure gene expression ratio of key genes |
| US8574836B2 (en) * | 2005-01-12 | 2013-11-05 | Ramesh Vallabhaneni | Method and apparatus for chromosome profiling |
| WO2009147537A2 (en) | 2008-05-27 | 2009-12-10 | Dako Denmark A/S | Compositions and methods for detection of chromosomal aberrations with novel hybridization buffers |
| EP2324123B1 (en) * | 2008-09-10 | 2016-07-20 | Rutgers, The State University of New Jersey | IMAGING INDIVIDUAL mRNA MOLECULES USING MULTIPLE SINGLY LABELED PROBES |
| EP2401395A1 (en) | 2009-02-26 | 2012-01-04 | Dako Denmark A/S | Compositions and methods for rna hybridization applications |
| WO2010147654A2 (en) | 2009-06-15 | 2010-12-23 | Netbio Inc. | Improved methods for forensic dna quantitation |
| EP3794548B1 (en) | 2018-05-15 | 2024-06-26 | Ventana Medical Systems, Inc. | Quantitation of signal in stain aggregates |
| US20230138540A1 (en) * | 2020-03-31 | 2023-05-04 | Personal Genome Diagnostics Inc. | Compositions and Methods for Nucleic Acid Quality Determination |
| CN115948608A (zh) * | 2022-08-05 | 2023-04-11 | 北京中杉金桥生物技术有限公司 | 一种用于eber检测的探针组及试剂盒 |
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|---|---|---|---|---|
| US3948922A (en) * | 1973-01-02 | 1976-04-06 | Lowe Orville G | Oxidation of thiols and disulfides to sulfonic acids |
| US5541308A (en) * | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| US5489507A (en) * | 1988-11-30 | 1996-02-06 | Perkin-Elmer Corporation | DNA detection by color complementation |
| US5324829A (en) * | 1988-12-16 | 1994-06-28 | Ortho Diagnostic Systems, Inc. | High specific activity nucleic acid probes having target recognition and signal generating moieties |
| US5789161A (en) * | 1990-09-20 | 1998-08-04 | Amoco Corporation | Methods for genome identification using direct label probe composition |
| US5843660A (en) * | 1994-09-30 | 1998-12-01 | Promega Corporation | Multiplex amplification of short tandem repeat loci |
| US5684142A (en) * | 1995-06-07 | 1997-11-04 | Oncor, Inc. | Modified nucleotides for nucleic acid labeling |
| US5871917A (en) * | 1996-05-31 | 1999-02-16 | North Shore University Hospital Research Corp. | Identification of differentially methylated and mutated nucleic acids |
| US7504493B2 (en) * | 1997-01-23 | 2009-03-17 | The John Hopkins University | Characterization of the yeast transcriptome |
| WO2002022874A2 (en) * | 2000-09-15 | 2002-03-21 | Ventana Medical Systems, Inc. | Oligonucleotides for labeling oligonucleotide probes and proteins |
| US7399339B2 (en) * | 2005-06-15 | 2008-07-15 | Gas Technology Institute | Polyoxometalate material for gaseous stream purification at high temperature |
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2001
- 2001-09-06 WO PCT/US2001/028014 patent/WO2002022874A2/en not_active Ceased
- 2001-09-06 AU AU9454201A patent/AU9454201A/xx active Pending
- 2001-09-06 AU AU2001294542A patent/AU2001294542B8/en not_active Expired
- 2001-09-06 EP EP01975189A patent/EP1366187B1/en not_active Expired - Lifetime
- 2001-09-06 DE DE60136746T patent/DE60136746D1/de not_active Expired - Lifetime
- 2001-09-06 AT AT01975189T patent/ATE415491T1/de active
- 2001-09-06 ES ES01975189T patent/ES2317939T3/es not_active Expired - Lifetime
- 2001-09-06 CA CA2422440A patent/CA2422440C/en not_active Expired - Lifetime
- 2001-09-06 CA CA2703141A patent/CA2703141C/en not_active Expired - Lifetime
- 2001-09-06 DK DK01975189T patent/DK1366187T3/da active
- 2001-09-06 US US10/380,584 patent/US7838655B2/en not_active Expired - Lifetime
- 2001-09-06 JP JP2002527314A patent/JP4986367B2/ja not_active Expired - Lifetime
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2010
- 2010-10-22 US US12/910,454 patent/US8236502B2/en not_active Expired - Fee Related
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2011
- 2011-10-24 JP JP2011233295A patent/JP5272063B2/ja not_active Expired - Lifetime
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2012
- 2012-06-28 US US13/536,021 patent/US8524877B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DE60136746D1 (de) | 2009-01-08 |
| AU9454201A (en) | 2002-03-26 |
| CA2703141A1 (en) | 2002-03-21 |
| JP2005503753A (ja) | 2005-02-10 |
| JP2012065660A (ja) | 2012-04-05 |
| AU2001294542B2 (en) | 2008-04-03 |
| EP1366187B1 (en) | 2008-11-26 |
| JP5272063B2 (ja) | 2013-08-28 |
| EP1366187A2 (en) | 2003-12-03 |
| WO2002022874A3 (en) | 2003-09-25 |
| CA2703141C (en) | 2014-10-21 |
| US20110034682A1 (en) | 2011-02-10 |
| ATE415491T1 (de) | 2008-12-15 |
| ES2317939T3 (es) | 2009-05-01 |
| AU2001294542B8 (en) | 2008-07-03 |
| WO2002022874A2 (en) | 2002-03-21 |
| JP4986367B2 (ja) | 2012-07-25 |
| CA2422440A1 (en) | 2002-03-21 |
| WO2002022874A9 (en) | 2003-04-03 |
| US20040014088A1 (en) | 2004-01-22 |
| US8236502B2 (en) | 2012-08-07 |
| WO2002022874A8 (en) | 2003-07-10 |
| DK1366187T3 (da) | 2009-02-16 |
| US20120309951A1 (en) | 2012-12-06 |
| US8524877B2 (en) | 2013-09-03 |
| US7838655B2 (en) | 2010-11-23 |
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| MKEX | Expiry |
Effective date: 20210907 |