JP2004523201A5 - - Google Patents
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- Publication number
- JP2004523201A5 JP2004523201A5 JP2001585298A JP2001585298A JP2004523201A5 JP 2004523201 A5 JP2004523201 A5 JP 2004523201A5 JP 2001585298 A JP2001585298 A JP 2001585298A JP 2001585298 A JP2001585298 A JP 2001585298A JP 2004523201 A5 JP2004523201 A5 JP 2004523201A5
- Authority
- JP
- Japan
- Prior art keywords
- probe
- sequence
- nucleic acid
- hybridization
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000523 sample Substances 0.000 description 78
- 238000009396 hybridization Methods 0.000 description 40
- 150000007523 nucleic acids Chemical class 0.000 description 37
- 108020004707 nucleic acids Proteins 0.000 description 26
- 102000039446 nucleic acids Human genes 0.000 description 26
- 238000000034 method Methods 0.000 description 24
- 108020004414 DNA Proteins 0.000 description 16
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 15
- 239000002853 nucleic acid probe Substances 0.000 description 15
- 125000003729 nucleotide group Chemical group 0.000 description 13
- 239000002773 nucleotide Substances 0.000 description 11
- 230000003252 repetitive effect Effects 0.000 description 11
- 210000000349 chromosome Anatomy 0.000 description 10
- 108091028043 Nucleic acid sequence Proteins 0.000 description 8
- 230000000295 complement effect Effects 0.000 description 8
- 239000007850 fluorescent dye Substances 0.000 description 7
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 6
- 101100384865 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cot-1 gene Proteins 0.000 description 5
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 230000002759 chromosomal effect Effects 0.000 description 4
- 239000012634 fragment Substances 0.000 description 4
- 239000013598 vector Substances 0.000 description 4
- 238000000137 annealing Methods 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- 238000007901 in situ hybridization Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 210000002230 centromere Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
- 230000031864 metaphase Effects 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000007899 nucleic acid hybridization Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 208000035970 Chromosome Breakpoints Diseases 0.000 description 1
- 238000000018 DNA microarray Methods 0.000 description 1
- 108010022894 Euchromatin Proteins 0.000 description 1
- 108060002716 Exonuclease Proteins 0.000 description 1
- 108010034791 Heterochromatin Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 238000002105 Southern blotting Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 108091092356 cellular DNA Proteins 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 229960000633 dextran sulfate Drugs 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 210000000632 euchromatin Anatomy 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 102000013165 exonuclease Human genes 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000012757 fluorescence staining Methods 0.000 description 1
- 238000004108 freeze drying Methods 0.000 description 1
- 210000004458 heterochromatin Anatomy 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000013383 initial experiment Methods 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 238000001668 nucleic acid synthesis Methods 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 108700022487 rRNA Genes Proteins 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 108020004418 ribosomal RNA Proteins 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/573,080 US6828097B1 (en) | 2000-05-16 | 2000-05-16 | Single copy genomic hybridization probes and method of generating same |
| US09/854,867 US7014997B2 (en) | 2000-05-16 | 2001-05-14 | Chromosome structural abnormality localization with single copy probes |
| PCT/US2001/015674 WO2001088089A2 (en) | 2000-05-16 | 2001-05-15 | Single copy genomic hybridization probes and method of generating same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2004523201A JP2004523201A (ja) | 2004-08-05 |
| JP2004523201A5 true JP2004523201A5 (enExample) | 2011-09-01 |
Family
ID=27076021
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001585298A Pending JP2004523201A (ja) | 2000-05-16 | 2001-05-15 | シングルコピーゲノムのハイブリダイゼーションプローブおよびその作製方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20080085509A1 (enExample) |
| EP (1) | EP1285093A4 (enExample) |
| JP (1) | JP2004523201A (enExample) |
| AU (2) | AU6461001A (enExample) |
| CA (1) | CA2409752A1 (enExample) |
| IL (1) | IL152727A0 (enExample) |
| NZ (2) | NZ539223A (enExample) |
| WO (1) | WO2001088089A2 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6828097B1 (en) | 2000-05-16 | 2004-12-07 | The Childrens Mercy Hospital | Single copy genomic hybridization probes and method of generating same |
| US7081339B2 (en) * | 2002-04-12 | 2006-07-25 | Primera Biosystems, Inc. | Methods for variation detection |
| KR20050073466A (ko) * | 2002-09-30 | 2005-07-13 | 칠드런스 머시 호스피탈 | 서브텔로미어 디엔에이 프로브 및 이의 제조 방법 |
| GB0326197D0 (en) * | 2003-11-10 | 2003-12-17 | Randox Lab Ltd | Molecular marker |
| CA2566395A1 (en) * | 2004-03-26 | 2005-10-13 | The Children's Mercy Hospital | Computational selection of probes for localizing chromosome breakpoints |
| US20060223075A1 (en) * | 2005-03-29 | 2006-10-05 | Exagen Diagnostics, Inc. | Unique sequence hybridization probes (USP) |
| US8407013B2 (en) | 2005-06-07 | 2013-03-26 | Peter K. Rogan | AB initio generation of single copy genomic probes |
| US7734424B1 (en) | 2005-06-07 | 2010-06-08 | Rogan Peter K | Ab initio generation of single copy genomic probes |
| AU2006327111A1 (en) * | 2005-11-18 | 2007-06-28 | The Children's Mercy Hospital | Mitigation of Cot-1 DNA distortion in nucleic acid hybridization |
| ES2372427T3 (es) | 2006-09-01 | 2012-01-19 | Ventana Medical Systems, Inc. | Método para producir sondas de ácido nucleico. |
| EP3276349B1 (en) | 2006-11-01 | 2019-08-07 | Ventana Medical Systems, Inc. | Haptens, hapten conjugates, compositions thereof and method for their preparation and use |
| WO2008119084A1 (en) * | 2007-03-28 | 2008-10-02 | The Children's Mercy Hospital | Method for identifying and selecting low copy nucleic acid segments |
| US7682789B2 (en) | 2007-05-04 | 2010-03-23 | Ventana Medical Systems, Inc. | Method for quantifying biomolecules conjugated to a nanoparticle |
| US7985557B2 (en) | 2007-05-23 | 2011-07-26 | Ventana Medical Systems, Inc. | Polymeric carriers for immunohistochemistry and in situ hybridization |
| CA2979353C (en) | 2008-06-05 | 2020-06-30 | Ventana Medical Systems, Inc. | Compositions comprising nanomaterials and method for using such compositions for histochemical processes |
| CN103003443B (zh) | 2009-11-23 | 2016-09-21 | 拜尔作物科学股份有限公司 | 耐除草剂大豆植物及鉴定其的方法 |
| CA2781557C (en) | 2009-11-23 | 2018-01-09 | Bayer Cropscience N.V. | Elite event ee-gm3 and methods and kits for identifying such event in biological samples |
| JP5838169B2 (ja) * | 2009-12-31 | 2016-01-06 | ヴェンタナ メディカル システムズ， インク． | 一意的に特異的核酸プローブを生成するための方法 |
| WO2016077426A1 (en) * | 2014-11-11 | 2016-05-19 | Abbott Molecular Inc. | Hybridization probes and methods |
| CN114350752B (zh) | 2015-07-17 | 2025-02-25 | 布鲁克空间生物学公司 | 在横切面组织的用户定义区域中的基因表达的同时定量 |
| WO2017214417A1 (en) * | 2016-06-10 | 2017-12-14 | Takara Bio Usa, Inc. | Methods and compositions employing blocked primers |
| US20230183780A1 (en) | 2016-07-25 | 2023-06-15 | InVivo BioTech Services GmbH | Dna probes for in situ hybridization on chromosomes |
| AU2017361521B2 (en) | 2016-11-21 | 2020-08-27 | Bruker Spatial Biology, Inc. | Chemical compositions and methods of using same |
| KR102893736B1 (ko) | 2018-05-14 | 2025-11-28 | 브루커 스페이셜 바이올로지, 인크. | 화학 조성물 및 이의 사용 방법 |
| EP3940075A1 (en) * | 2020-07-17 | 2022-01-19 | Istituto Nazionale Di Genetica Molecolare-INGM | Inhibitors of line1 and uses thereof |
| CN113483931B (zh) * | 2021-07-02 | 2023-01-24 | 中国国家铁路集团有限公司 | 弓网接触力区段异常识别方法及装置 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5110920A (en) | 1982-01-22 | 1992-05-05 | Cetus Corporation | HLA typing method and DNA probes used therein |
| US5985549A (en) | 1985-10-22 | 1999-11-16 | University Of Massachusetts | Non-isotopic in-situ hybridization method for detection of nucleic acids |
| US5721098A (en) * | 1986-01-16 | 1998-02-24 | The Regents Of The University Of California | Comparative genomic hybridization |
| US5756696A (en) * | 1986-01-16 | 1998-05-26 | Regents Of The University Of California | Compositions for chromosome-specific staining |
| US5447841A (en) | 1986-01-16 | 1995-09-05 | The Regents Of The Univ. Of California | Methods for chromosome-specific staining |
| US5273881A (en) | 1990-05-07 | 1993-12-28 | Daikin Industries, Ltd. | Diagnostic applications of double D-loop formation |
| US6040140A (en) * | 1991-12-11 | 2000-03-21 | Thomas Jefferson University | Methods for screening and treating leukemias resulting from all-1 region chromosome abnormalities |
| US6121419A (en) * | 1992-06-17 | 2000-09-19 | Arch Development Corp. | Compositions and methods for detecting gene rearrangements and translocations |
| WO1994017208A1 (en) * | 1993-01-21 | 1994-08-04 | President And Fellows Of Harvard College | Methods and diagnostic kits utilizing mammalian stress promoters to determine toxicity of a compound |
| US6150160A (en) * | 1995-11-16 | 2000-11-21 | The John Hopkins University | Compositions and methods of use of mammalian retrotransposons |
| US6222029B1 (en) * | 1997-08-01 | 2001-04-24 | Genset | 5′ ESTs for secreted proteins expressed in brain |
| US6828097B1 (en) * | 2000-05-16 | 2004-12-07 | The Childrens Mercy Hospital | Single copy genomic hybridization probes and method of generating same |
| WO2002093130A2 (en) * | 2001-05-14 | 2002-11-21 | Cancer Genetics, Inc. | Methods of analyzing chromosomal translocations using fluorescence in situ hybridization (fish) |
-
2001
- 2001-05-15 AU AU6461001A patent/AU6461001A/xx active Pending
- 2001-05-15 IL IL15272701A patent/IL152727A0/xx unknown
- 2001-05-15 JP JP2001585298A patent/JP2004523201A/ja active Pending
- 2001-05-15 WO PCT/US2001/015674 patent/WO2001088089A2/en not_active Ceased
- 2001-05-15 CA CA002409752A patent/CA2409752A1/en not_active Abandoned
- 2001-05-15 EP EP01939048A patent/EP1285093A4/en not_active Withdrawn
- 2001-05-15 AU AU2001264610A patent/AU2001264610B2/en not_active Ceased
- 2001-05-15 NZ NZ539223A patent/NZ539223A/en unknown
- 2001-05-15 NZ NZ522406A patent/NZ522406A/xx unknown
-
2004
- 2004-06-23 US US10/876,297 patent/US20080085509A1/en not_active Abandoned
-
2009
- 2009-03-25 US US12/411,359 patent/US20090312533A1/en not_active Abandoned
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