ES2317939T3 - Oligonucleotidos para marcar sondas oligonucleotidicas y proteinas. - Google Patents
Oligonucleotidos para marcar sondas oligonucleotidicas y proteinas. Download PDFInfo
- Publication number
- ES2317939T3 ES2317939T3 ES01975189T ES01975189T ES2317939T3 ES 2317939 T3 ES2317939 T3 ES 2317939T3 ES 01975189 T ES01975189 T ES 01975189T ES 01975189 T ES01975189 T ES 01975189T ES 2317939 T3 ES2317939 T3 ES 2317939T3
- Authority
- ES
- Spain
- Prior art keywords
- baselineskip
- artificial sequence
- oligonucleotide probe
- dna
- hskip1cm
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 78
- 102000004169 proteins and genes Human genes 0.000 title description 8
- 239000000523 sample Substances 0.000 claims abstract description 184
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims abstract description 170
- 239000002751 oligonucleotide probe Substances 0.000 claims abstract description 170
- 239000003550 marker Substances 0.000 claims description 31
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 claims description 28
- 108020004999 messenger RNA Proteins 0.000 claims description 21
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical group O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims description 15
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 13
- 229940104302 cytosine Drugs 0.000 claims description 11
- 230000003252 repetitive effect Effects 0.000 claims description 7
- 229920000642 polymer Polymers 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 3
- 102000012960 Immunoglobulin kappa-Chains Human genes 0.000 claims 1
- 108010090227 Immunoglobulin kappa-Chains Proteins 0.000 claims 1
- 229910052757 nitrogen Inorganic materials 0.000 claims 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims 1
- 238000007901 in situ hybridization Methods 0.000 abstract description 81
- 238000004458 analytical method Methods 0.000 abstract description 54
- 150000007523 nucleic acids Chemical class 0.000 abstract description 25
- 108020004707 nucleic acids Proteins 0.000 abstract description 19
- 102000039446 nucleic acids Human genes 0.000 abstract description 19
- 108020004414 DNA Proteins 0.000 description 128
- 210000001519 tissue Anatomy 0.000 description 57
- 210000004027 cell Anatomy 0.000 description 36
- 238000009396 hybridization Methods 0.000 description 21
- 108091034117 Oligonucleotide Proteins 0.000 description 19
- 238000001514 detection method Methods 0.000 description 18
- 241000701022 Cytomegalovirus Species 0.000 description 17
- 238000002372 labelling Methods 0.000 description 17
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 15
- 108060003951 Immunoglobulin Proteins 0.000 description 15
- 238000013461 design Methods 0.000 description 15
- 102000018358 immunoglobulin Human genes 0.000 description 15
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 11
- 238000000034 method Methods 0.000 description 11
- 239000002773 nucleotide Substances 0.000 description 10
- 108020003217 Nuclear RNA Proteins 0.000 description 9
- 102000043141 Nuclear RNA Human genes 0.000 description 9
- 125000003729 nucleotide group Chemical group 0.000 description 9
- 230000003393 splenic effect Effects 0.000 description 9
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 8
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 8
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 8
- 230000001413 cellular effect Effects 0.000 description 7
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 6
- 230000014509 gene expression Effects 0.000 description 6
- 210000002741 palatine tonsil Anatomy 0.000 description 6
- 239000012188 paraffin wax Substances 0.000 description 5
- OALHHIHQOFIMEF-UHFFFAOYSA-N 3',6'-dihydroxy-2',4',5',7'-tetraiodo-3h-spiro[2-benzofuran-1,9'-xanthene]-3-one Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC(I)=C(O)C(I)=C1OC1=C(I)C(O)=C(I)C=C21 OALHHIHQOFIMEF-UHFFFAOYSA-N 0.000 description 4
- 206010025323 Lymphomas Diseases 0.000 description 4
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 4
- 229960002685 biotin Drugs 0.000 description 4
- 235000020958 biotin Nutrition 0.000 description 4
- 239000011616 biotin Substances 0.000 description 4
- YPHMISFOHDHNIV-FSZOTQKASA-N cycloheximide Chemical compound C1[C@@H](C)C[C@H](C)C(=O)[C@@H]1[C@H](O)CC1CC(=O)NC(=O)C1 YPHMISFOHDHNIV-FSZOTQKASA-N 0.000 description 4
- 239000002853 nucleic acid probe Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- SHIBSTMRCDJXLN-UHFFFAOYSA-N Digoxigenin Natural products C1CC(C2C(C3(C)CCC(O)CC3CC2)CC2O)(O)C2(C)C1C1=CC(=O)OC1 SHIBSTMRCDJXLN-UHFFFAOYSA-N 0.000 description 3
- 229920004890 Triton X-100 Polymers 0.000 description 3
- 239000013504 Triton X-100 Substances 0.000 description 3
- 238000013459 approach Methods 0.000 description 3
- 102000023732 binding proteins Human genes 0.000 description 3
- 108091008324 binding proteins Proteins 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- QONQRTHLHBTMGP-UHFFFAOYSA-N digitoxigenin Natural products CC12CCC(C3(CCC(O)CC3CC3)C)C3C11OC1CC2C1=CC(=O)OC1 QONQRTHLHBTMGP-UHFFFAOYSA-N 0.000 description 3
- SHIBSTMRCDJXLN-KCZCNTNESA-N digoxigenin Chemical compound C1([C@@H]2[C@@]3([C@@](CC2)(O)[C@H]2[C@@H]([C@@]4(C)CC[C@H](O)C[C@H]4CC2)C[C@H]3O)C)=CC(=O)OC1 SHIBSTMRCDJXLN-KCZCNTNESA-N 0.000 description 3
- 239000003085 diluting agent Substances 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000010186 staining Methods 0.000 description 3
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- IEQAICDLOKRSRL-UHFFFAOYSA-N 2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-dodecoxyethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethanol Chemical compound CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO IEQAICDLOKRSRL-UHFFFAOYSA-N 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 101710180319 Protease 1 Proteins 0.000 description 2
- 239000006146 Roswell Park Memorial Institute medium Substances 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- 101710137710 Thioesterase 1/protease 1/lysophospholipase L1 Proteins 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 210000000805 cytoplasm Anatomy 0.000 description 2
- 229960002086 dextran Drugs 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 2
- 229940072221 immunoglobulins Drugs 0.000 description 2
- 238000003364 immunohistochemistry Methods 0.000 description 2
- 238000010249 in-situ analysis Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- QEIFSLUFHRCVQL-UHFFFAOYSA-N (5-bromo-4-chloro-1h-indol-3-yl) hydrogen phosphate;(4-methylphenyl)azanium Chemical compound CC1=CC=C(N)C=C1.C1=C(Br)C(Cl)=C2C(OP(O)(=O)O)=CNC2=C1 QEIFSLUFHRCVQL-UHFFFAOYSA-N 0.000 description 1
- GXVUZYLYWKWJIM-UHFFFAOYSA-N 2-(2-aminoethoxy)ethanamine Chemical compound NCCOCCN GXVUZYLYWKWJIM-UHFFFAOYSA-N 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- WOVKYSAHUYNSMH-RRKCRQDMSA-N 5-bromodeoxyuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(Br)=C1 WOVKYSAHUYNSMH-RRKCRQDMSA-N 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 101100314454 Caenorhabditis elegans tra-1 gene Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 241000701024 Human betaherpesvirus 5 Species 0.000 description 1
- 102000013463 Immunoglobulin Light Chains Human genes 0.000 description 1
- 108010065825 Immunoglobulin Light Chains Proteins 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 108091093105 Nuclear DNA Proteins 0.000 description 1
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 1
- FIXOJBWBMIHSDW-UHFFFAOYSA-N P(=O)([O-])([O-])[O-].BrC=1N(N(N[NH+]1)C=1NC2=CC=CC=C2C1)Cl.BrC=1N(N(N[NH+]1)C=1NC2=CC=CC=C2C1)Cl.BrC=1N(N(N[NH+]1)C=1NC2=CC=CC=C2C1)Cl Chemical compound P(=O)([O-])([O-])[O-].BrC=1N(N(N[NH+]1)C=1NC2=CC=CC=C2C1)Cl.BrC=1N(N(N[NH+]1)C=1NC2=CC=CC=C2C1)Cl.BrC=1N(N(N[NH+]1)C=1NC2=CC=CC=C2C1)Cl FIXOJBWBMIHSDW-UHFFFAOYSA-N 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 108020004518 RNA Probes Proteins 0.000 description 1
- 239000003391 RNA probe Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000021615 conjugation Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229960000633 dextran sulfate Drugs 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 208000016361 genetic disease Diseases 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 238000002991 immunohistochemical analysis Methods 0.000 description 1
- 230000002055 immunohistochemical effect Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 125000005647 linker group Chemical group 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- FSVCQIDHPKZJSO-UHFFFAOYSA-L nitro blue tetrazolium dichloride Chemical compound [Cl-].[Cl-].COC1=CC(C=2C=C(OC)C(=CC=2)[N+]=2N(N=C(N=2)C=2C=CC=CC=2)C=2C=CC(=CC=2)[N+]([O-])=O)=CC=C1[N+]1=NC(C=2C=CC=CC=2)=NN1C1=CC=C([N+]([O-])=O)C=C1 FSVCQIDHPKZJSO-UHFFFAOYSA-L 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000003141 primary amines Chemical class 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 125000000467 secondary amino group Chemical class [H]N([*:1])[*:2] 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000008096 xylene Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US23317700P | 2000-09-15 | 2000-09-15 | |
| US233177P | 2000-09-15 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2317939T3 true ES2317939T3 (es) | 2009-05-01 |
Family
ID=22876207
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES01975189T Expired - Lifetime ES2317939T3 (es) | 2000-09-15 | 2001-09-06 | Oligonucleotidos para marcar sondas oligonucleotidicas y proteinas. |
Country Status (10)
| Country | Link |
|---|---|
| US (3) | US7838655B2 (enExample) |
| EP (1) | EP1366187B1 (enExample) |
| JP (2) | JP4986367B2 (enExample) |
| AT (1) | ATE415491T1 (enExample) |
| AU (2) | AU9454201A (enExample) |
| CA (2) | CA2703141C (enExample) |
| DE (1) | DE60136746D1 (enExample) |
| DK (1) | DK1366187T3 (enExample) |
| ES (1) | ES2317939T3 (enExample) |
| WO (1) | WO2002022874A2 (enExample) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2703141C (en) * | 2000-09-15 | 2014-10-21 | Ventana Medical Systems, Inc. | Oligonucleotide sequence formula for labeling oligonucleotide probes and proteins for in situ analysis |
| BR0210094A (pt) * | 2001-06-06 | 2004-04-13 | Canag Diagnostics Ab | Método para medir a proporção de expressão de um gene da classe de genes padrões |
| US8574836B2 (en) * | 2005-01-12 | 2013-11-05 | Ramesh Vallabhaneni | Method and apparatus for chromosome profiling |
| WO2009144581A1 (en) | 2008-05-27 | 2009-12-03 | Dako Denmark A/S | Hybridization compositions and methods |
| AU2009291752B2 (en) * | 2008-09-10 | 2015-12-17 | Rutgers, The State University Of New Jersey | Imaging individual mRNA molecules using multiple singly labeled probes |
| WO2010097655A1 (en) | 2009-02-26 | 2010-09-02 | Dako Denmark A/S | Compositions and methods for rna hybridization applications |
| EP2443254A2 (en) | 2009-06-15 | 2012-04-25 | NetBio, Inc. | Improved methods for forensic dna quantitation |
| CN112424825B (zh) | 2018-05-15 | 2025-01-07 | 文塔纳医疗系统公司 | 染色聚集体中信号的定量 |
| KR20220160633A (ko) * | 2020-03-31 | 2022-12-06 | 퍼스널 게놈 다이아그노스틱스 인코포레이티드 | 핵산 품질 결정을 위한 조성물 및 방법 |
| CN115948608A (zh) * | 2022-08-05 | 2023-04-11 | 北京中杉金桥生物技术有限公司 | 一种用于eber检测的探针组及试剂盒 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3948922A (en) * | 1973-01-02 | 1976-04-06 | Lowe Orville G | Oxidation of thiols and disulfides to sulfonic acids |
| US5541308A (en) * | 1986-11-24 | 1996-07-30 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| US5489507A (en) * | 1988-11-30 | 1996-02-06 | Perkin-Elmer Corporation | DNA detection by color complementation |
| US5324829A (en) * | 1988-12-16 | 1994-06-28 | Ortho Diagnostic Systems, Inc. | High specific activity nucleic acid probes having target recognition and signal generating moieties |
| US5789161A (en) * | 1990-09-20 | 1998-08-04 | Amoco Corporation | Methods for genome identification using direct label probe composition |
| US5843660A (en) * | 1994-09-30 | 1998-12-01 | Promega Corporation | Multiplex amplification of short tandem repeat loci |
| US5684142A (en) * | 1995-06-07 | 1997-11-04 | Oncor, Inc. | Modified nucleotides for nucleic acid labeling |
| US5871917A (en) * | 1996-05-31 | 1999-02-16 | North Shore University Hospital Research Corp. | Identification of differentially methylated and mutated nucleic acids |
| US7504493B2 (en) * | 1997-01-23 | 2009-03-17 | The John Hopkins University | Characterization of the yeast transcriptome |
| CA2703141C (en) * | 2000-09-15 | 2014-10-21 | Ventana Medical Systems, Inc. | Oligonucleotide sequence formula for labeling oligonucleotide probes and proteins for in situ analysis |
| US7399339B2 (en) * | 2005-06-15 | 2008-07-15 | Gas Technology Institute | Polyoxometalate material for gaseous stream purification at high temperature |
-
2001
- 2001-09-06 CA CA2703141A patent/CA2703141C/en not_active Expired - Lifetime
- 2001-09-06 CA CA2422440A patent/CA2422440C/en not_active Expired - Lifetime
- 2001-09-06 DK DK01975189T patent/DK1366187T3/da active
- 2001-09-06 WO PCT/US2001/028014 patent/WO2002022874A2/en not_active Ceased
- 2001-09-06 AU AU9454201A patent/AU9454201A/xx active Pending
- 2001-09-06 US US10/380,584 patent/US7838655B2/en not_active Expired - Lifetime
- 2001-09-06 EP EP01975189A patent/EP1366187B1/en not_active Expired - Lifetime
- 2001-09-06 AU AU2001294542A patent/AU2001294542B8/en not_active Expired
- 2001-09-06 AT AT01975189T patent/ATE415491T1/de active
- 2001-09-06 JP JP2002527314A patent/JP4986367B2/ja not_active Expired - Lifetime
- 2001-09-06 ES ES01975189T patent/ES2317939T3/es not_active Expired - Lifetime
- 2001-09-06 DE DE60136746T patent/DE60136746D1/de not_active Expired - Lifetime
-
2010
- 2010-10-22 US US12/910,454 patent/US8236502B2/en not_active Expired - Fee Related
-
2011
- 2011-10-24 JP JP2011233295A patent/JP5272063B2/ja not_active Expired - Lifetime
-
2012
- 2012-06-28 US US13/536,021 patent/US8524877B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| AU2001294542B2 (en) | 2008-04-03 |
| WO2002022874A8 (en) | 2003-07-10 |
| CA2703141A1 (en) | 2002-03-21 |
| WO2002022874A2 (en) | 2002-03-21 |
| JP5272063B2 (ja) | 2013-08-28 |
| AU2001294542B8 (en) | 2008-07-03 |
| ATE415491T1 (de) | 2008-12-15 |
| CA2703141C (en) | 2014-10-21 |
| JP2005503753A (ja) | 2005-02-10 |
| CA2422440A1 (en) | 2002-03-21 |
| US7838655B2 (en) | 2010-11-23 |
| DK1366187T3 (da) | 2009-02-16 |
| US20110034682A1 (en) | 2011-02-10 |
| JP2012065660A (ja) | 2012-04-05 |
| EP1366187B1 (en) | 2008-11-26 |
| WO2002022874A9 (en) | 2003-04-03 |
| US20040014088A1 (en) | 2004-01-22 |
| US20120309951A1 (en) | 2012-12-06 |
| AU9454201A (en) | 2002-03-26 |
| EP1366187A2 (en) | 2003-12-03 |
| CA2422440C (en) | 2010-07-27 |
| US8524877B2 (en) | 2013-09-03 |
| US8236502B2 (en) | 2012-08-07 |
| DE60136746D1 (de) | 2009-01-08 |
| JP4986367B2 (ja) | 2012-07-25 |
| WO2002022874A3 (en) | 2003-09-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5272063B2 (ja) | イン・サイチュー解析のためのオリゴヌクレオチドプローブおよびタンパク質を標識するためのオリゴヌクレオチド | |
| US20250277257A1 (en) | Hybridization compositions and methods | |
| ES2562821T3 (es) | Método ultrasensible para la detección in situ de ácidos nucleicos | |
| US9388456B2 (en) | Compositions and methods for performing a stringent wash step in hybridization applications | |
| Koji et al. | Localization in situ of specific mRNA using thymine‐thymine dimerized DNA probes: sensitive and reliable non‐radioactive in situ hybridization | |
| ES2601237T3 (es) | Composiciones y métodos para realizar hibridaciones sin desnaturalización | |
| EP1356116A2 (en) | Hybridization buffers using low molecular weight dextran sulfate and methods for their use | |
| AU2002240180A1 (en) | Hybridization buffers using low molecular weight dextran sulfate and methods for their use | |
| JP2005503753A5 (enExample) | ||
| AU2001294542A1 (en) | Oligonucleotide sequence formula for labeling oligonucleotide probes and proteins for in-situ analysis | |
| JP7068469B2 (ja) | 大麻草ｄｎａを検出するためのプライマー対、キット及び方法 | |
| ES2845073T3 (es) | Composiciones de hibridación y métodos |