CA2418502A1 - Nanoparticules comportant des oligonucleotides fixes dessus et utilisations associees - Google Patents

Nanoparticules comportant des oligonucleotides fixes dessus et utilisations associees Download PDF

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Publication number
CA2418502A1
CA2418502A1 CA002418502A CA2418502A CA2418502A1 CA 2418502 A1 CA2418502 A1 CA 2418502A1 CA 002418502 A CA002418502 A CA 002418502A CA 2418502 A CA2418502 A CA 2418502A CA 2418502 A1 CA2418502 A1 CA 2418502A1
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CA
Canada
Prior art keywords
oligonucleotides
nanoparticles
nucleic acid
bound
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002418502A
Other languages
English (en)
Inventor
Chad A. Mirkin
Robert L. Letsinger
Robert C. Mucic
James J. Storhoff
Robert Elghanian
Thomas Andrew Taton
Viswanadham Garimella
Zhi Li
So-Jung Park
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanosphere LLC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/760,500 external-priority patent/US6767702B2/en
Priority claimed from US09/820,279 external-priority patent/US6750016B2/en
Application filed by Individual filed Critical Individual
Publication of CA2418502A1 publication Critical patent/CA2418502A1/fr
Abandoned legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Nanotechnology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Composite Materials (AREA)
  • Materials Engineering (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Saccharide Compounds (AREA)

Abstract

L'invention concerne des procédés de détection d'acide nucléique. Ces procédés consistent à mettre en contact l'acide nucléique avec un ou plusieurs types de particules sur lesquels sont attachés des oligonucléotides. Dans une réalisation du procédé, les oligonucléotides sont attachés à des nanoparticules et contiennent des séquences complémentaires des portions de la séquence de l'acide nucléique. Un changement détectable (de préférence un changement de couleur) s'opère comme résultat de l'hybridation entre l'acide nucléique et les oligonucléotides portés par les nanoparticules. L'invention concerne en outre des compositions et des nécessaires contenant des nanoparticules. L'invention concerne aussi des procédés de synthèse de conjugués uniques nanoparticule-oligonucléotide, les conjugués produits à l'aide de ces procédés, et des procédés d'utilisation de ces conjugués. Elle concerne encore des nanomatériaux et des nanostructures comprenant des nanoparticules ainsi que des procédés de nanofabrication utilisant ces nanoparticules. Elle concerne enfin un procédé de séparation d'un acide nucléique sélectionné parmi d'autres acides nucléiques.
CA002418502A 2000-08-11 2001-08-10 Nanoparticules comportant des oligonucleotides fixes dessus et utilisations associees Abandoned CA2418502A1 (fr)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
US22463100P 2000-08-11 2000-08-11
US60/224,631 2000-08-11
US25439200P 2000-12-08 2000-12-08
US60/254,392 2000-12-08
US25523500P 2000-12-11 2000-12-11
US60/255,235 2000-12-11
US09/760,500 2001-01-12
US09/760,500 US6767702B2 (en) 1996-07-29 2001-01-12 Nanoparticles having oligonucleotides attached thereto and uses therefor
US09/820,279 US6750016B2 (en) 1996-07-29 2001-03-28 Nanoparticles having oligonucleotides attached thereto and uses therefor
US09/820,279 2001-03-28
PCT/US2001/025237 WO2002018643A2 (fr) 2000-08-11 2001-08-10 Nanoparticules comportant des oligonucleotides fixes dessus et utilisations associees

Publications (1)

Publication Number Publication Date
CA2418502A1 true CA2418502A1 (fr) 2002-03-07

Family

ID=27539880

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002418502A Abandoned CA2418502A1 (fr) 2000-08-11 2001-08-10 Nanoparticules comportant des oligonucleotides fixes dessus et utilisations associees

Country Status (5)

Country Link
EP (1) EP1362121A2 (fr)
JP (1) JP2004525607A (fr)
AU (1) AU2001281248A1 (fr)
CA (1) CA2418502A1 (fr)
WO (1) WO2002018643A2 (fr)

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Also Published As

Publication number Publication date
JP2004525607A (ja) 2004-08-26
EP1362121A2 (fr) 2003-11-19
WO2002018643A3 (fr) 2003-09-04
WO2002018643A9 (fr) 2003-11-06
AU2001281248A1 (en) 2002-03-13
WO2002018643A2 (fr) 2002-03-07

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