CA2402352C - Fluorescent membrane intercalating probes and methods for their use - Google Patents

Fluorescent membrane intercalating probes and methods for their use Download PDF

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Publication number
CA2402352C
CA2402352C CA2402352A CA2402352A CA2402352C CA 2402352 C CA2402352 C CA 2402352C CA 2402352 A CA2402352 A CA 2402352A CA 2402352 A CA2402352 A CA 2402352A CA 2402352 C CA2402352 C CA 2402352C
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CA
Canada
Prior art keywords
cells
compound
cell
probes
use according
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2402352A
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English (en)
French (fr)
Other versions
CA2402352A1 (en
Inventor
Brian D. Gray
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Phanos Tech Inc
Original Assignee
Phanos Tech Inc
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Filing date
Publication date
Application filed by Phanos Tech Inc filed Critical Phanos Tech Inc
Publication of CA2402352A1 publication Critical patent/CA2402352A1/en
Application granted granted Critical
Publication of CA2402352C publication Critical patent/CA2402352C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K49/00—Preparations for testing in vivo
    • A61K49/001—Preparation for luminescence or biological staining
    • A61K49/0013—Luminescence
    • A61K49/0017—Fluorescence in vivo
    • A61K49/0019—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules
    • A61K49/0021—Fluorescence in vivo characterised by the fluorescent group, e.g. oligomeric, polymeric or dendritic molecules the fluorescent group being a small organic molecule
    • A61K49/0032—Methine dyes, e.g. cyanine dyes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/10—Indoles; Hydrogenated indoles with substituted hydrocarbon radicals attached to carbon atoms of the hetero ring
    • C07D209/14—Radicals substituted by nitrogen atoms, not forming part of a nitro radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/56—Ring systems containing three or more rings
    • C07D209/58—[b]- or [c]-condensed
    • C07D209/60—Naphtho [b] pyrroles; Hydrogenated naphtho [b] pyrroles
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D215/00—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems
    • C07D215/02—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom
    • C07D215/12—Heterocyclic compounds containing quinoline or hydrogenated quinoline ring systems having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen atoms or carbon atoms directly attached to the ring nitrogen atom with substituted hydrocarbon radicals attached to ring carbon atoms
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/0008—Methine or polymethine dyes, e.g. cyanine dyes substituted on the polymethine chain
    • C09B23/0016—Methine or polymethine dyes, e.g. cyanine dyes substituted on the polymethine chain the substituent being a halogen atom
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/0066—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain being part of a carbocyclic ring,(e.g. benzene, naphtalene, cyclohexene, cyclobutenene-quadratic acid)
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/083—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines five >CH- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C09B23/08—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines
    • C09B23/086—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups more than three >CH- groups, e.g. polycarbocyanines more than five >CH- groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Biomedical Technology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Indole Compounds (AREA)
  • Investigating Materials By The Use Of Optical Means Adapted For Particular Applications (AREA)
CA2402352A 2000-03-03 2001-03-05 Fluorescent membrane intercalating probes and methods for their use Expired - Lifetime CA2402352C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18668200P 2000-03-03 2000-03-03
US60/186,682 2000-03-03
PCT/US2001/006923 WO2001066153A1 (en) 2000-03-03 2001-03-05 Fluorescent membrane intercalating probes and methods for their use

Publications (2)

Publication Number Publication Date
CA2402352A1 CA2402352A1 (en) 2001-09-13
CA2402352C true CA2402352C (en) 2012-08-28

Family

ID=22685883

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2402352A Expired - Lifetime CA2402352C (en) 2000-03-03 2001-03-05 Fluorescent membrane intercalating probes and methods for their use

Country Status (8)

Country Link
US (2) US7462347B2 (https=)
EP (1) EP1265640B1 (https=)
JP (1) JP2003525915A (https=)
AT (1) ATE429932T1 (https=)
AU (2) AU4542401A (https=)
CA (1) CA2402352C (https=)
DE (1) DE60138531D1 (https=)
WO (1) WO2001066153A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2665628C1 (ru) * 2018-01-25 2018-09-03 Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр онкологии имени Н.Н. Блохина" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ онкологии им. Н.Н. Блохина" Минздрава России) Устройство для спектрально-флуоресцентного исследования содержания флуорохромов

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU4542401A (en) * 2000-03-03 2001-09-17 Phanos Tech Inc Fluorescent membrane intercalating probes and methods for their use
AU2005234696B2 (en) * 2005-11-18 2012-02-16 Phanos Technologies, Inc. Fluorescent membrane intercalating probes and methods for their use
US20150223675A1 (en) * 2012-09-04 2015-08-13 Given Imaging Ltd. Luminal administration of tag molecules for diagnostic applications
CA2901116C (en) * 2012-09-05 2021-11-09 Randall Thomas Irvin Methods and composition for detecting intestinal cell-barrier dysfunction
US9034596B1 (en) 2014-04-17 2015-05-19 Kuwait University Method for fluorescent staining of cellular and intracellular membranes
CN104098500B (zh) * 2014-07-08 2016-03-16 中国科学院烟台海岸带研究所 基于花青的有机化合物及其应用
US10663473B2 (en) 2016-02-02 2020-05-26 Maximus Diagnostic Technologies Llc Method of identifying a status of a mucosal intestinal epithelial barrier to improve treatment of inflammatory bowel disease
US20210181185A1 (en) * 2017-11-09 2021-06-17 Mindimmune Therapeutics, Inc. Dendritic cell recruitment from blood to brain in neurodegenerative disease

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4762701A (en) * 1986-10-31 1988-08-09 Smithkline Beckman Corporation In vivo cellular tracking
US4783401A (en) * 1986-10-31 1988-11-08 Smithkline Beckman Corporation Viable cell labelling
US4859584A (en) * 1986-10-31 1989-08-22 Smithkline Beckman Corporation Cell growth rate determination by measurement of changes in cyanine dye levels in plasma membranes
JP3038339B2 (ja) * 1988-05-02 2000-05-08 ザイナクシス・テクノロジーズ・インコーポレーテッド バイオ粒子の表面膜に対してバイオアフェクティング物質を結合する化合物
KR100397020B1 (ko) * 1995-01-30 2004-04-29 다이이치 가가쿠 야쿠힝 가부시키가이샤 진단용마커
JP3669752B2 (ja) * 1995-01-30 2005-07-13 第一化学薬品株式会社 診断用マーカー
US6004536A (en) * 1995-11-14 1999-12-21 Molecular Probes, Inc. Lipophilic cyanine dyes with enchanced aqueous solubilty
US5804389A (en) * 1995-12-29 1998-09-08 Phanos Technologies, Inc. Method for detecting abnormal epithelial cell shedding
US6027709A (en) * 1997-01-10 2000-02-22 Li-Cor Inc. Fluorescent cyanine dyes
AU4542401A (en) * 2000-03-03 2001-09-17 Phanos Tech Inc Fluorescent membrane intercalating probes and methods for their use

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2665628C1 (ru) * 2018-01-25 2018-09-03 Федеральное государственное бюджетное учреждение "Национальный медицинский исследовательский центр онкологии имени Н.Н. Блохина" Министерства здравоохранения Российской Федерации (ФГБУ "НМИЦ онкологии им. Н.Н. Блохина" Минздрава России) Устройство для спектрально-флуоресцентного исследования содержания флуорохромов

Also Published As

Publication number Publication date
DE60138531D1 (de) 2009-06-10
WO2001066153A1 (en) 2001-09-13
US8029767B2 (en) 2011-10-04
US20100003196A1 (en) 2010-01-07
AU4542401A (en) 2001-09-17
EP1265640B1 (en) 2009-04-29
AU2001245424B2 (en) 2005-08-18
EP1265640A4 (en) 2003-07-09
US7462347B2 (en) 2008-12-09
ATE429932T1 (de) 2009-05-15
US20060193780A1 (en) 2006-08-31
JP2003525915A (ja) 2003-09-02
EP1265640A1 (en) 2002-12-18
CA2402352A1 (en) 2001-09-13

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