CA2400441C - Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof - Google Patents

Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof Download PDF

Info

Publication number
CA2400441C
CA2400441C CA2400441A CA2400441A CA2400441C CA 2400441 C CA2400441 C CA 2400441C CA 2400441 A CA2400441 A CA 2400441A CA 2400441 A CA2400441 A CA 2400441A CA 2400441 C CA2400441 C CA 2400441C
Authority
CA
Canada
Prior art keywords
sequence
cel
dna
endonuclease
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2400441A
Other languages
English (en)
French (fr)
Other versions
CA2400441A1 (en
Inventor
Anthony T. Yeung
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute for Cancer Research
Original Assignee
Fox Chase Cancer Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/510,322 external-priority patent/US6391557B1/en
Priority claimed from US09/514,768 external-priority patent/US6699980B1/en
Application filed by Fox Chase Cancer Center filed Critical Fox Chase Cancer Center
Publication of CA2400441A1 publication Critical patent/CA2400441A1/en
Application granted granted Critical
Publication of CA2400441C publication Critical patent/CA2400441C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2400441A 2000-02-22 2001-02-22 Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof Expired - Lifetime CA2400441C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/510,322 2000-02-22
US09/510,322 US6391557B1 (en) 2000-02-22 2000-02-22 Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
US09/514,768 US6699980B1 (en) 2000-02-28 2000-02-28 Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
US09/514,768 2000-02-28
PCT/US2001/005502 WO2001062974A1 (en) 2000-02-22 2001-02-22 Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof

Publications (2)

Publication Number Publication Date
CA2400441A1 CA2400441A1 (en) 2001-08-30
CA2400441C true CA2400441C (en) 2013-08-27

Family

ID=27056866

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2400441A Expired - Lifetime CA2400441C (en) 2000-02-22 2001-02-22 Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof

Country Status (5)

Country Link
EP (1) EP1257671B1 (https=)
JP (1) JP4986358B2 (https=)
AU (2) AU2001247212B2 (https=)
CA (1) CA2400441C (https=)
WO (1) WO2001062974A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
CA2436214C (en) 2001-02-02 2012-12-04 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide
US7838219B2 (en) 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
US7078211B2 (en) 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
DE10248258A1 (de) * 2002-10-16 2004-05-06 Biopsytec Analytik Gmbh Mutierte Nukleinsäure einer Cel I-Endonuklease und Verfahren zur Herstellung des rekombinanten vollständigen Cel I-Proteins
CA2575276A1 (en) * 2004-07-30 2006-02-02 Institut National De La Recherche Agronomique Method for producing highly sensitive endonucleases, novel preparations of endonucleases and uses thereof
CN101538561B (zh) * 2009-04-17 2010-09-29 广州大学 一种提取芹菜中celⅰ核酸酶的方法
JP6009649B2 (ja) 2013-03-14 2016-10-19 タカラバイオ株式会社 耐熱性のミスマッチエンドヌクレアーゼの利用方法
KR20170053636A (ko) * 2014-09-11 2017-05-16 다카라 바이오 가부시키가이샤 내열성의 미스매치 엔도뉴클레아제의 이용방법
WO2016178207A1 (en) 2015-05-04 2016-11-10 Ramot At Tel-Aviv University Ltd. Methods and kits for fragmenting dna
CN119242611A (zh) 2020-03-19 2025-01-03 宝生物工程株式会社 耐热的错配核酸内切酶变体
CN112301020B (zh) * 2020-10-19 2024-04-12 复旦大学附属肿瘤医院 Iii类脱氧核酶突变体及其制备方法与应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0964929B1 (en) * 1996-06-05 2004-10-20 Fox Chase Cancer Center Mismatch endonucleases and uses thereof in identifying mutations in targeted polynucleotide strands
US5869245A (en) * 1996-06-05 1999-02-09 Fox Chase Cancer Center Mismatch endonuclease and its use in identifying mutations in targeted polynucleotide strands

Also Published As

Publication number Publication date
EP1257671B1 (en) 2015-01-14
EP1257671A4 (en) 2005-08-17
EP1257671A1 (en) 2002-11-20
JP4986358B2 (ja) 2012-07-25
JP2004529602A (ja) 2004-09-30
CA2400441A1 (en) 2001-08-30
AU4721201A (en) 2001-09-03
AU2001247212B2 (en) 2005-12-22
WO2001062974A1 (en) 2001-08-30

Similar Documents

Publication Publication Date Title
US6391557B1 (en) Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
Yang et al. Purification, cloning, and characterization of the CEL I nuclease
Tischfield et al. Low-molecular-weight, calcium-dependent phospholipase A2genes are linked and map to homologous chromosome regions in mouse and human
JP3159708B2 (ja) アンジオテンシノーゲン遺伝子変異体および高血圧素質
Hamajima et al. Dihydropyrimidinase deficiency: structural organization, chromosomal localization, and mutation analysis of the human dihydropyrimidinase gene
CA2400441C (en) Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
WO1996022364A1 (en) Compositions and methods to promote homologous recombination in eukaryotic cells and organisms
JP4213214B2 (ja) ミスマッチエンドヌクレアーゼおよび標的ポリヌクレオチド鎖中突然変異の同定におけるその使用
AU2001247212A1 (en) Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
US20080090763A1 (en) Novel gene encoding a dna repair enzyme and methods of use thereof
Zhao et al. Characterization and genomic mapping of genes and pseudogenes of a new human protein tyrosine phosphatase
JP4031199B2 (ja) ヒト気道トリプシン様酵素遺伝子多型による疾病体質解析
US5856095A (en) Identification of two novel mutant alleles of human thiopurine S-methyltransferase, and diagnostic uses thereof
JP2008507965A (ja) 高感受性エンドヌクレアーゼを生産する方法、新規なエンドヌクレアーゼ調製物、及びそれらの使用
US6699980B1 (en) Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
US20140322711A1 (en) Spinocerebellar ataxia type 8 and methods of detection
Mole et al. Molecular genetics of the neuronal ceroid lipofuscinoses
US6046009A (en) Diagnosis and treatment of glaucoma
JP5424519B2 (ja) パーキン遺伝子の突然変異、組成物、方法及び使用
US5605797A (en) Bovine β-mannosidase gene and methods of use
US6660847B1 (en) Rheumatoid arthritis gene
CA2409705A1 (en) Gene responsible for stargardt-like dominant macular dystrophy
AU730917B2 (en) A genetic test for alpha-mannosidosis
WO1997007201A1 (en) Identification of two novel mutant alleles of human thiopurine s-methyltransferase, and diagnostic uses thereof
Hamshere et al. Myotonic dystrophy

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20210222