JP4986358B2 - ミスマッチエンドヌクレアーゼをコードする核酸分子およびその使用方法 - Google Patents

ミスマッチエンドヌクレアーゼをコードする核酸分子およびその使用方法 Download PDF

Info

Publication number
JP4986358B2
JP4986358B2 JP2001561782A JP2001561782A JP4986358B2 JP 4986358 B2 JP4986358 B2 JP 4986358B2 JP 2001561782 A JP2001561782 A JP 2001561782A JP 2001561782 A JP2001561782 A JP 2001561782A JP 4986358 B2 JP4986358 B2 JP 4986358B2
Authority
JP
Japan
Prior art keywords
cel
sequence
nucleic acid
dna
seq
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2001561782A
Other languages
English (en)
Japanese (ja)
Other versions
JP2004529602A5 (https=
JP2004529602A (ja
Inventor
アンソニー・ティ・ユング
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fox Chase Cancer Center
Original Assignee
Fox Chase Cancer Center
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/510,322 external-priority patent/US6391557B1/en
Priority claimed from US09/514,768 external-priority patent/US6699980B1/en
Application filed by Fox Chase Cancer Center filed Critical Fox Chase Cancer Center
Publication of JP2004529602A publication Critical patent/JP2004529602A/ja
Publication of JP2004529602A5 publication Critical patent/JP2004529602A5/ja
Application granted granted Critical
Publication of JP4986358B2 publication Critical patent/JP4986358B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2001561782A 2000-02-22 2001-02-22 ミスマッチエンドヌクレアーゼをコードする核酸分子およびその使用方法 Expired - Fee Related JP4986358B2 (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US09/510,322 2000-02-22
US09/510,322 US6391557B1 (en) 2000-02-22 2000-02-22 Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
US09/514,768 US6699980B1 (en) 2000-02-28 2000-02-28 Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
US09/514,768 2000-02-28
PCT/US2001/005502 WO2001062974A1 (en) 2000-02-22 2001-02-22 Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof

Publications (3)

Publication Number Publication Date
JP2004529602A JP2004529602A (ja) 2004-09-30
JP2004529602A5 JP2004529602A5 (https=) 2008-04-10
JP4986358B2 true JP4986358B2 (ja) 2012-07-25

Family

ID=27056866

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2001561782A Expired - Fee Related JP4986358B2 (ja) 2000-02-22 2001-02-22 ミスマッチエンドヌクレアーゼをコードする核酸分子およびその使用方法

Country Status (5)

Country Link
EP (1) EP1257671B1 (https=)
JP (1) JP4986358B2 (https=)
AU (2) AU2001247212B2 (https=)
CA (1) CA2400441C (https=)
WO (1) WO2001062974A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7582423B2 (en) 2001-02-02 2009-09-01 Novici Biotech Llc Population of polynucleotide sequence variants
CA2436214C (en) 2001-02-02 2012-12-04 Large Scale Biology Corporation A method of increasing complementarity in a heteroduplex polynucleotide
US7838219B2 (en) 2001-02-02 2010-11-23 Novici Biotech Llc Method of increasing complementarity in a heteroduplex
US7078211B2 (en) 2002-02-01 2006-07-18 Large Scale Biology Corporation Nucleic acid molecules encoding endonucleases and methods of use thereof
DE10248258A1 (de) * 2002-10-16 2004-05-06 Biopsytec Analytik Gmbh Mutierte Nukleinsäure einer Cel I-Endonuklease und Verfahren zur Herstellung des rekombinanten vollständigen Cel I-Proteins
CA2575276A1 (en) * 2004-07-30 2006-02-02 Institut National De La Recherche Agronomique Method for producing highly sensitive endonucleases, novel preparations of endonucleases and uses thereof
CN101538561B (zh) * 2009-04-17 2010-09-29 广州大学 一种提取芹菜中celⅰ核酸酶的方法
JP6009649B2 (ja) 2013-03-14 2016-10-19 タカラバイオ株式会社 耐熱性のミスマッチエンドヌクレアーゼの利用方法
KR20170053636A (ko) * 2014-09-11 2017-05-16 다카라 바이오 가부시키가이샤 내열성의 미스매치 엔도뉴클레아제의 이용방법
WO2016178207A1 (en) 2015-05-04 2016-11-10 Ramot At Tel-Aviv University Ltd. Methods and kits for fragmenting dna
CN119242611A (zh) 2020-03-19 2025-01-03 宝生物工程株式会社 耐热的错配核酸内切酶变体
CN112301020B (zh) * 2020-10-19 2024-04-12 复旦大学附属肿瘤医院 Iii类脱氧核酶突变体及其制备方法与应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997046701A1 (en) * 1996-06-05 1997-12-11 Fox Chase Cancer Center Mismatch endonucleases and uses thereof in identifying mutations in targeted polynucleotide strands

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5869245A (en) * 1996-06-05 1999-02-09 Fox Chase Cancer Center Mismatch endonuclease and its use in identifying mutations in targeted polynucleotide strands

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997046701A1 (en) * 1996-06-05 1997-12-11 Fox Chase Cancer Center Mismatch endonucleases and uses thereof in identifying mutations in targeted polynucleotide strands

Also Published As

Publication number Publication date
CA2400441C (en) 2013-08-27
EP1257671B1 (en) 2015-01-14
EP1257671A4 (en) 2005-08-17
EP1257671A1 (en) 2002-11-20
JP2004529602A (ja) 2004-09-30
CA2400441A1 (en) 2001-08-30
AU4721201A (en) 2001-09-03
AU2001247212B2 (en) 2005-12-22
WO2001062974A1 (en) 2001-08-30

Similar Documents

Publication Publication Date Title
AU2016216734B2 (en) Maize cytoplasmic male sterility (CMS) C-type restorer RF4 gene, molecular markers and their use
AU716362B2 (en) Compositions and methods to promote homologous recombination in eukaryotic cells and organisms
Kouzminova et al. dim‐2 encodes a DNA methyltransferase responsible for all known cytosine methylation in Neurospora
CN110592089B (zh) 用于切割靶dna的组合物及其用途
JP4101285B2 (ja) Ｄｎａミスマッチ修復遺伝子に関する組成物および方法
US6391557B1 (en) Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
JP4986358B2 (ja) ミスマッチエンドヌクレアーゼをコードする核酸分子およびその使用方法
US20120009583A1 (en) Method for Producing Highly Sensitive Endonucleases, Novel Preparations of Endonucleases and Uses Thereof
JP4213214B2 (ja) ミスマッチエンドヌクレアーゼおよび標的ポリヌクレオチド鎖中突然変異の同定におけるその使用
US20080090763A1 (en) Novel gene encoding a dna repair enzyme and methods of use thereof
KR100828506B1 (ko) 마우스 정자 형성 유전자와 인간 남성 불임 관련 유전자,및 이들을 사용한 진단 시스템
CN114774399A (zh) 一种人工改造脱氨酶辅助的dna中5-羟甲基胞嘧啶修饰单碱基分辨率定位分析方法
AU2001247212A1 (en) Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
US20140322711A1 (en) Spinocerebellar ataxia type 8 and methods of detection
JP3514779B2 (ja) アポリポプロテインｅ遺伝子タイプの検査方法及びその検査に好適なプライマー及びプローブ
US6699980B1 (en) Nucleic acid molecule encoding a mismatch endonuclease and methods of use thereof
US5605797A (en) Bovine β-mannosidase gene and methods of use
JP2000507807A (ja) 組換えバクテリオファージｔ４エンドヌクレアーゼｖｉｉの過剰発現およびその使用
Wisotzkey et al. α/β hydrolase2, a predicated gene adjacent to mad in Drosophila melanogaster, belongs to a new global multigene family and is associated with obesity
Hamshere et al. Myotonic dystrophy
US7122328B2 (en) Gene involved in mineral deposition and uses thereof
JPH09504437A (ja) 誤対合修復系を利用したｄｎａの解析方法および操作方法
HK40018765B (en) Composition for cleaving a target dna and use thereof
HK40012112B (zh) 用於切割靶dna的组合物及其用途
HK40020375B (zh) 用於切割靶dna的组合物及其用途

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20080222

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20080222

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20101026

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20110126

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20110405

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20110704

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20110711

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20110804

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20110811

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20110902

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20110909

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20111005

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20120327

A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20120424

R150 Certificate of patent or registration of utility model

Free format text: JAPANESE INTERMEDIATE CODE: R150

FPAY Renewal fee payment (event date is renewal date of database)

Free format text: PAYMENT UNTIL: 20150511

Year of fee payment: 3

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees