CA2393733A1 - Procedes et compositions se rapportant a la detection electrique des reactions d'acides nucleiques - Google Patents

Procedes et compositions se rapportant a la detection electrique des reactions d'acides nucleiques Download PDF

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Publication number
CA2393733A1
CA2393733A1 CA002393733A CA2393733A CA2393733A1 CA 2393733 A1 CA2393733 A1 CA 2393733A1 CA 002393733 A CA002393733 A CA 002393733A CA 2393733 A CA2393733 A CA 2393733A CA 2393733 A1 CA2393733 A1 CA 2393733A1
Authority
CA
Canada
Prior art keywords
microelectrodes
electrode
contact
nucleic acid
target molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002393733A
Other languages
English (en)
Inventor
Vi-En Choong
Sean Gallagher
Mike Gaskin
Changming Li
George Maracas
Song Shi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Motorola Solutions Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/458,533 external-priority patent/US20020051975A1/en
Priority claimed from US09/459,685 external-priority patent/US6518024B2/en
Application filed by Individual filed Critical Individual
Publication of CA2393733A1 publication Critical patent/CA2393733A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

L'invention se rapporte à la détection d'interactions moléculaires entre les molécules biologiques. Elle concerne plus particulièrement la détection électrique d'interactions telles que l'hybridation entre différents acides nucléiques ou d'interaction antigène - anticorps de peptides utilisant des réseaux de peptides et d'oligonucléotides. Elle concerne un appareil et des procédés pour détecter la liaison de peptides ou l'hybridation d'acides nucléiques au moyen de procédés électriques, y compris l'impédance du c.a. Dans certains modes de réalisation, on n'utilise aucun groupe fonctionnel électrochimique ou autre groupe fonctionnel d'étiquetage. Dans d'autres, on utilise des étiquettes à activité électrochimique pour détecter des réactions sur des réseaux d'hydrogel, y compris des réactions de génotypage telles que la réaction d'extension de base unique.
CA002393733A 1999-12-09 2000-12-11 Procedes et compositions se rapportant a la detection electrique des reactions d'acides nucleiques Abandoned CA2393733A1 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US45850199A 1999-12-09 1999-12-09
US09/458,501 1999-12-09
US09/458,533 1999-12-09
US09/458,533 US20020051975A1 (en) 1999-12-09 1999-12-09 Reporterless genosensors using electrical detection methods
US09/459,685 1999-12-13
US09/459,685 US6518024B2 (en) 1999-12-13 1999-12-13 Electrochemical detection of single base extension
PCT/US2000/033497 WO2001042508A2 (fr) 1999-12-09 2000-12-11 Procedes et compositions se rapportant a la detection electrique des reactions d'acides nucleiques

Publications (1)

Publication Number Publication Date
CA2393733A1 true CA2393733A1 (fr) 2001-06-14

Family

ID=27412734

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002393733A Abandoned CA2393733A1 (fr) 1999-12-09 2000-12-11 Procedes et compositions se rapportant a la detection electrique des reactions d'acides nucleiques

Country Status (5)

Country Link
EP (1) EP1238114A2 (fr)
JP (1) JP2003516165A (fr)
AU (1) AU2907201A (fr)
CA (1) CA2393733A1 (fr)
WO (1) WO2001042508A2 (fr)

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US6071699A (en) 1996-06-07 2000-06-06 California Institute Of Technology Nucleic acid mediated electron transfer
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GB0118931D0 (en) * 2001-08-02 2001-09-26 Oxford Biosensors Ltd Voltammetric ion-selective biosensor
DE10210051B4 (de) 2002-03-07 2005-10-13 Siemens Ag Vorrichtung zum elektrochemischen Nachweis einer Nukleotidsequenz, Analyse-Kassette für eine solche Vorrichtung und Verfahren zur Herstellung einer solchen Analyse-Kassette
DE10224567B4 (de) 2002-06-03 2014-10-23 Boehringer Ingelheim Vetmedica Gmbh Sensor-Anordnung und Verfahren zum Betreiben einer Sensor-Anordnung
DE10228260A1 (de) * 2002-06-25 2004-01-22 Bayer Ag Methode und Vorrichtung zum impedimetrischen Nachweis eines oder mehrerer Analyten in einer Probe
GB2391867A (en) * 2002-08-13 2004-02-18 Smartbead Technologies Ltd Analysis system using coded supports
KR100858080B1 (ko) 2002-11-12 2008-09-10 삼성전자주식회사 전기적 신호를 측정하는 ｐｃｒ 증폭 산물을 검출하는 방법
WO2004044570A1 (fr) * 2002-11-14 2004-05-27 Toyama Prefecture Procede de detection d'hybridation
DE10259819B4 (de) * 2002-12-19 2006-07-13 Siemens Ag Verfahren zur PCR-Amplifikation und Detektion von Nucleotidsequenzen
WO2005016115A2 (fr) 2003-01-23 2005-02-24 Montana State University Biocapteurs faisant intervenir des ligands immobilises sur des dendrimeres et utilisation de ceux-ci
AT413214B (de) * 2003-01-24 2005-12-15 Sy Lab Vgmbh Messanordnung und verfahren zur detektion einer dna-sequenz
WO2005001122A2 (fr) * 2003-06-27 2005-01-06 Adnavance Technologies, Inc. Detection electrochimique de liaison d'adn
EP1608775A1 (fr) * 2003-03-05 2005-12-28 University of Saskatchewan Technologies Inc. Detection de mesappariement d'acides nucleiques
DE10327756B4 (de) * 2003-06-18 2005-12-29 november Aktiengesellschaft Gesellschaft für Molekulare Medizin Verfahren zum Echtzeit-Quantifizieren einer Nukleinsäure
KR100590569B1 (ko) * 2004-02-14 2006-06-15 삼성전자주식회사 실시간 중합효소연쇄반응 검사 방법
US20080227651A1 (en) * 2004-03-25 2008-09-18 Yanxiu Zhou Cyclic voltammetry (CV) for identifying genomic sequence variations and detecting mismatch base pairs, such as single nucleotide polymorphisms
WO2005093418A1 (fr) * 2004-03-26 2005-10-06 Toto, Ltd. Procede de detection specifique de substance de test a l'aide de courant photoelectrique et d'electrodes, cellule de mesure, dispositif de mesure et solution tampon destines a etre utilises a cet effet
SG151334A1 (en) 2004-04-01 2009-04-30 Univ Nanyang Addressable chem/bio chip array
KR100613612B1 (ko) 2004-04-27 2006-08-18 삼성전자주식회사 인덕턴스 소자 및 캐패시턴스 소자를 이용한 바이오결합검출 장치 및 방법
JP2008527999A (ja) 2005-01-21 2008-07-31 ユニバーシティー オブ ロチェスター 長い１本鎖核酸および２本鎖核酸から短い１本鎖核酸を分離するための方法、ならびに関連した生体分子アッセイ法
WO2009041917A1 (fr) * 2007-09-28 2009-04-02 Agency For Science, Technology And Research Procédé de détection électrique d'une molécule d'acide nucléique
SG189839A1 (en) 2010-10-04 2013-06-28 Genapsys Inc Systems and methods for automated reusable parallel biological reactions
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
EP2785868B1 (fr) 2011-12-01 2017-04-12 Genapsys Inc. Systèmes et procédés pour le séquençage et la détection électroniques à haut rendement
WO2014152625A1 (fr) 2013-03-15 2014-09-25 Genapsys, Inc. Systèmes et procédés pour l'analyse biologique
WO2015089238A1 (fr) 2013-12-11 2015-06-18 Genapsys, Inc. Systèmes et procédés d'analyse et d'informatique biologiques
WO2015161054A2 (fr) 2014-04-18 2015-10-22 Genapsys, Inc. Procédés et systèmes pour l'amplification d'acide nucléique
CN116397014A (zh) 2016-07-20 2023-07-07 测序健康公司 用于核酸测序的系统和方法
CN111566224A (zh) 2017-09-21 2020-08-21 吉纳普赛斯股份有限公司 用于核酸测序的系统和方法

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Also Published As

Publication number Publication date
EP1238114A2 (fr) 2002-09-11
AU2907201A (en) 2001-06-18
JP2003516165A (ja) 2003-05-13
WO2001042508A3 (fr) 2002-03-14
WO2001042508A9 (fr) 2002-05-30
WO2001042508A2 (fr) 2001-06-14

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Legal Events

Date Code Title Description
FZDE Discontinued