CA2386090A1 - Production de genes et de proteines hybrides fonctionnels - Google Patents

Production de genes et de proteines hybrides fonctionnels Download PDF

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Publication number
CA2386090A1
CA2386090A1 CA002386090A CA2386090A CA2386090A1 CA 2386090 A1 CA2386090 A1 CA 2386090A1 CA 002386090 A CA002386090 A CA 002386090A CA 2386090 A CA2386090 A CA 2386090A CA 2386090 A1 CA2386090 A1 CA 2386090A1
Authority
CA
Canada
Prior art keywords
polynucleotide
parent
construct
gene
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002386090A
Other languages
English (en)
Inventor
Frances Arnold
Volker Sieber
Ji-Hu Zhang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
California Institute of Technology CalTech
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2386090A1 publication Critical patent/CA2386090A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'invention concerne un procédé amélioré de création de bibliothèques de gènes et de protéines, en particulier de bibliothèques de gènes aléatoires codant pour des protéines hybrides contenant des fragments d'une ou de deux protéines parentes. Le procédé permet de produire des bibliothèques de variants de gènes à permutation circulaire codant pour une protéine simple ou des protéines hybrides, notamment des protéines à chaîne simple. En outre, on peut également produire des bibliothèques de complémentation de protéines-fragments dans lesquelles les deux fragments proviennent d'une ou de deux protéines parentes. Le procédé permet de produire une bibliothèque de gènes de taille généralement correcte, menant à une fraction élevée d'hybrides ou de compléments fonctionnels. Couplé à un criblage ou une sélection approprié, le procédé permet de créer et d'identifier des protéines hybrides, y compris de nouvelles possédant des propriétés nouvelles ou modifiées. L'invention concerne également des bibliothèques de protéines hybrides, notamment des protéines à chaîne simple, qui renferment une séquence N-terminal provenant d'une protéine parente, fusionnée à une séquence C-terminal d'une seconde protéine parente, la longueur des deux séquences variant entre les hybrides de la bibliothèque.
CA002386090A 1999-10-27 2000-10-27 Production de genes et de proteines hybrides fonctionnels Abandoned CA2386090A1 (fr)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US16185299P 1999-10-27 1999-10-27
US16185099P 1999-10-27 1999-10-27
US60/161,850 1999-10-27
US60/161,852 1999-10-27
US67262900A 2000-09-28 2000-09-28
US67285900A 2000-09-28 2000-09-28
US09/672,859 2000-09-28
US09/672,629 2000-09-28
PCT/US2000/029717 WO2001030998A1 (fr) 1999-10-27 2000-10-27 Production de genes et de proteines hybrides fonctionnels

Publications (1)

Publication Number Publication Date
CA2386090A1 true CA2386090A1 (fr) 2001-05-03

Family

ID=27496481

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002386090A Abandoned CA2386090A1 (fr) 1999-10-27 2000-10-27 Production de genes et de proteines hybrides fonctionnels

Country Status (4)

Country Link
EP (1) EP1228200A1 (fr)
AU (1) AU1350801A (fr)
CA (1) CA2386090A1 (fr)
WO (1) WO2001030998A1 (fr)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US5830696A (en) 1996-12-05 1998-11-03 Diversa Corporation Directed evolution of thermophilic enzymes
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US6790605B1 (en) 1995-12-07 2004-09-14 Diversa Corporation Methods for obtaining a desired bioactivity or biomolecule using DNA libraries from an environmental source
US6939689B2 (en) 1995-12-07 2005-09-06 Diversa Corporation Exonuclease-mediated nucleic acid reassembly in directed evolution
EP1276858A1 (fr) * 2000-04-21 2003-01-22 Genencor International, Inc. Reconstruction d'acides nucleiques n'utilisant pas la pcr
GB0119366D0 (en) * 2001-08-08 2001-10-03 Imperial College Enzymes and enzymic processes
DE102010056289A1 (de) * 2010-12-24 2012-06-28 Geneart Ag Verfahren zur Herstellung von Leseraster-korrekten Fragment-Bibliotheken
US9200291B2 (en) 2012-12-19 2015-12-01 Helge Zieler Compositions and methods for creating altered and improved cells and organisms
DK2935582T3 (da) * 2012-12-19 2020-03-02 Helge Zieler Sammensætninger og fremgangsmåder til dannelse af ændrede og forbedrede celler og organismer

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5969105A (en) * 1996-10-25 1999-10-19 Feng; Yiqing Stem cell factor receptor agonists
EP1115854A1 (fr) * 1998-09-25 2001-07-18 G.D. Searle & Co. Procede de production de permuteines par permutagenese d'exploration

Also Published As

Publication number Publication date
EP1228200A1 (fr) 2002-08-07
WO2001030998A1 (fr) 2001-05-03
AU1350801A (en) 2001-05-08
WO2001030998A9 (fr) 2002-05-30

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Legal Events

Date Code Title Description
FZDE Discontinued