CA2379165A1 - Chimeric proteins - Google Patents
Chimeric proteins Download PDFInfo
- Publication number
- CA2379165A1 CA2379165A1 CA002379165A CA2379165A CA2379165A1 CA 2379165 A1 CA2379165 A1 CA 2379165A1 CA 002379165 A CA002379165 A CA 002379165A CA 2379165 A CA2379165 A CA 2379165A CA 2379165 A1 CA2379165 A1 CA 2379165A1
- Authority
- CA
- Canada
- Prior art keywords
- protein
- interaction
- nucleic acid
- sequence
- chimeric protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 102000037865 fusion proteins Human genes 0.000 title claims description 67
- 108020001507 fusion proteins Proteins 0.000 title claims description 67
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 297
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 259
- 230000000694 effects Effects 0.000 claims abstract description 141
- 238000001668 nucleic acid synthesis Methods 0.000 claims abstract description 69
- 230000003993 interaction Effects 0.000 claims abstract description 65
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims abstract description 63
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims abstract description 63
- 150000007523 nucleic acids Chemical class 0.000 claims description 76
- 108020004707 nucleic acids Proteins 0.000 claims description 71
- 102000039446 nucleic acids Human genes 0.000 claims description 71
- 238000000034 method Methods 0.000 claims description 54
- 108020004414 DNA Proteins 0.000 claims description 52
- 239000013598 vector Substances 0.000 claims description 45
- 238000003752 polymerase chain reaction Methods 0.000 claims description 42
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 claims description 28
- 238000006243 chemical reaction Methods 0.000 claims description 27
- 210000004027 cell Anatomy 0.000 claims description 23
- 239000002773 nucleotide Substances 0.000 claims description 23
- 125000003729 nucleotide group Chemical group 0.000 claims description 23
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 18
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 18
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 18
- 108060002716 Exonuclease Proteins 0.000 claims description 17
- 102000013165 exonuclease Human genes 0.000 claims description 17
- 230000015572 biosynthetic process Effects 0.000 claims description 15
- 238000010839 reverse transcription Methods 0.000 claims description 14
- 230000001419 dependent effect Effects 0.000 claims description 12
- 230000003321 amplification Effects 0.000 claims description 10
- 230000000295 complement effect Effects 0.000 claims description 10
- 239000005546 dideoxynucleotide Substances 0.000 claims description 10
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 10
- 238000012163 sequencing technique Methods 0.000 claims description 10
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 9
- 102100034343 Integrase Human genes 0.000 claims description 9
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 9
- 238000009396 hybridization Methods 0.000 claims description 9
- 108091028664 Ribonucleotide Proteins 0.000 claims description 7
- 238000002372 labelling Methods 0.000 claims description 7
- 239000011541 reaction mixture Substances 0.000 claims description 7
- 239000002336 ribonucleotide Substances 0.000 claims description 7
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 5
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims description 4
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims description 4
- 210000004899 c-terminal region Anatomy 0.000 claims description 4
- 238000010348 incorporation Methods 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 3
- 102000009609 Pyrophosphatases Human genes 0.000 claims description 2
- 108010009413 Pyrophosphatases Proteins 0.000 claims description 2
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 239000013604 expression vector Substances 0.000 claims description 2
- 235000018102 proteins Nutrition 0.000 description 241
- 230000027455 binding Effects 0.000 description 45
- 235000001014 amino acid Nutrition 0.000 description 30
- 150000001413 amino acids Chemical class 0.000 description 29
- 241000205042 Archaeoglobus fulgidus Species 0.000 description 28
- 108050006400 Cyclin Proteins 0.000 description 26
- 102000009339 Proliferating Cell Nuclear Antigen Human genes 0.000 description 26
- 239000012634 fragment Substances 0.000 description 23
- 238000001086 yeast two-hybrid system Methods 0.000 description 17
- 230000010076 replication Effects 0.000 description 16
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 15
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 15
- 238000000338 in vitro Methods 0.000 description 13
- 238000001727 in vivo Methods 0.000 description 13
- 230000008878 coupling Effects 0.000 description 12
- 238000010168 coupling process Methods 0.000 description 12
- 238000005859 coupling reaction Methods 0.000 description 12
- 108091035707 Consensus sequence Proteins 0.000 description 11
- 125000003275 alpha amino acid group Chemical group 0.000 description 11
- 238000012217 deletion Methods 0.000 description 11
- 230000037430 deletion Effects 0.000 description 11
- 238000010396 two-hybrid screening Methods 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 10
- 238000013518 transcription Methods 0.000 description 10
- 230000035897 transcription Effects 0.000 description 10
- 108010071146 DNA Polymerase III Proteins 0.000 description 9
- 102000007528 DNA Polymerase III Human genes 0.000 description 9
- 230000004568 DNA-binding Effects 0.000 description 9
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 230000004913 activation Effects 0.000 description 8
- 241000894006 Bacteria Species 0.000 description 7
- 108010006785 Taq Polymerase Proteins 0.000 description 7
- 230000008859 change Effects 0.000 description 7
- 230000035772 mutation Effects 0.000 description 7
- 238000003786 synthesis reaction Methods 0.000 description 7
- 230000004543 DNA replication Effects 0.000 description 6
- 241000203353 Methanococcus Species 0.000 description 6
- 238000004925 denaturation Methods 0.000 description 6
- 230000036425 denaturation Effects 0.000 description 6
- 230000004927 fusion Effects 0.000 description 6
- 102000004196 processed proteins & peptides Human genes 0.000 description 6
- GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 5
- 229930024421 Adenine Natural products 0.000 description 5
- 241000203069 Archaea Species 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 5
- 241001148023 Pyrococcus abyssi Species 0.000 description 5
- 241000522615 Pyrococcus horikoshii Species 0.000 description 5
- 241000589596 Thermus Species 0.000 description 5
- 229960000643 adenine Drugs 0.000 description 5
- 238000010276 construction Methods 0.000 description 5
- HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 5
- 239000013641 positive control Substances 0.000 description 5
- 230000008439 repair process Effects 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 4
- 241000206602 Eukaryota Species 0.000 description 4
- 102100039556 Galectin-4 Human genes 0.000 description 4
- 241000282414 Homo sapiens Species 0.000 description 4
- HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 108010022394 Threonine synthase Proteins 0.000 description 4
- 238000013459 approach Methods 0.000 description 4
- 102000004419 dihydrofolate reductase Human genes 0.000 description 4
- 210000001840 diploid cell Anatomy 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000013507 mapping Methods 0.000 description 4
- 239000013612 plasmid Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 101150084750 1 gene Proteins 0.000 description 3
- 108020004635 Complementary DNA Proteins 0.000 description 3
- 101150009006 HIS3 gene Proteins 0.000 description 3
- 101000608765 Homo sapiens Galectin-4 Proteins 0.000 description 3
- 241000205160 Pyrococcus Species 0.000 description 3
- 108700008625 Reporter Genes Proteins 0.000 description 3
- 241000589500 Thermus aquaticus Species 0.000 description 3
- 238000010804 cDNA synthesis Methods 0.000 description 3
- 239000002299 complementary DNA Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000010494 dissociation reaction Methods 0.000 description 3
- 230000005593 dissociations Effects 0.000 description 3
- 239000002777 nucleoside Substances 0.000 description 3
- 238000002864 sequence alignment Methods 0.000 description 3
- 241000567139 Aeropyrum pernix Species 0.000 description 2
- 241000893512 Aquifex aeolicus Species 0.000 description 2
- 101150071330 CDC48 gene Proteins 0.000 description 2
- 101100483666 Caenorhabditis elegans ufd-3 gene Proteins 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
- 230000033616 DNA repair Effects 0.000 description 2
- 238000001712 DNA sequencing Methods 0.000 description 2
- 101100369138 Danio rerio vcp gene Proteins 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 241000203407 Methanocaldococcus jannaschii Species 0.000 description 2
- 241001302042 Methanothermobacter thermautotrophicus Species 0.000 description 2
- 238000005481 NMR spectroscopy Methods 0.000 description 2
- 241000205156 Pyrococcus furiosus Species 0.000 description 2
- 102000018779 Replication Protein C Human genes 0.000 description 2
- 108010027647 Replication Protein C Proteins 0.000 description 2
- 101100394989 Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) hisI gene Proteins 0.000 description 2
- 241000293869 Salmonella enterica subsp. enterica serovar Typhimurium Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 239000002269 analeptic agent Substances 0.000 description 2
- 108010005774 beta-Galactosidase Proteins 0.000 description 2
- 230000003197 catalytic effect Effects 0.000 description 2
- 210000003855 cell nucleus Anatomy 0.000 description 2
- 241000902900 cellular organisms Species 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 108020001580 protein domains Proteins 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- 230000019491 signal transduction Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 238000012916 structural analysis Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000013519 translation Methods 0.000 description 2
- 241001515965 unidentified phage Species 0.000 description 2
- 101150096273 ADE2 gene Proteins 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 241000589969 Borreliella burgdorferi Species 0.000 description 1
- 241001429558 Caldicellulosiruptor bescii Species 0.000 description 1
- 241000620141 Carboxydothermus Species 0.000 description 1
- 101710160937 DNA replication protein Proteins 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 102100027700 DNA-directed RNA polymerase I subunit RPA2 Human genes 0.000 description 1
- 241000724228 Enterobacteria phage RB69 Species 0.000 description 1
- 241000701832 Enterobacteria phage T3 Species 0.000 description 1
- 108010058643 Fungal Proteins Proteins 0.000 description 1
- 108010001515 Galectin 4 Proteins 0.000 description 1
- 241000193385 Geobacillus stearothermophilus Species 0.000 description 1
- 241000590002 Helicobacter pylori Species 0.000 description 1
- 102100021628 Histatin-3 Human genes 0.000 description 1
- 101000650600 Homo sapiens DNA-directed RNA polymerase I subunit RPA2 Proteins 0.000 description 1
- 101000898505 Homo sapiens Histatin-3 Proteins 0.000 description 1
- 101001072338 Homo sapiens Proliferating cell nuclear antigen Proteins 0.000 description 1
- 101001092206 Homo sapiens Replication protein A 32 kDa subunit Proteins 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241000508310 Meiothermus chliarophilus Species 0.000 description 1
- 241000589496 Meiothermus ruber Species 0.000 description 1
- 241000508289 Meiothermus silvanus Species 0.000 description 1
- -1 Mg2+ ion Chemical class 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 241000588770 Proteus mirabilis Species 0.000 description 1
- 241000589776 Pseudomonas putida Species 0.000 description 1
- 108010019653 Pwo polymerase Proteins 0.000 description 1
- 241001278922 Pyrococcus glycovorans Species 0.000 description 1
- 241000204670 Pyrodictium occultum Species 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 238000003559 RNA-seq method Methods 0.000 description 1
- 102000018780 Replication Protein A Human genes 0.000 description 1
- 108010027643 Replication Protein A Proteins 0.000 description 1
- 101100117360 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) DOA1 gene Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 101710142606 Sliding clamp Proteins 0.000 description 1
- 241000187432 Streptomyces coelicolor Species 0.000 description 1
- 241000204103 Thermococcus fumicolans Species 0.000 description 1
- 241001237851 Thermococcus gorgonarius Species 0.000 description 1
- 241000205180 Thermococcus litoralis Species 0.000 description 1
- 241000206213 Thermosipho africanus Species 0.000 description 1
- 241000204664 Thermotoga neapolitana Species 0.000 description 1
- 241000589501 Thermus caldophilus Species 0.000 description 1
- 241000589498 Thermus filiformis Species 0.000 description 1
- 241000557726 Thermus oshimai Species 0.000 description 1
- 241001522143 Thermus scotoductus Species 0.000 description 1
- 102000002933 Thioredoxin Human genes 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 108010027273 Valosin Containing Protein Proteins 0.000 description 1
- 241000193758 [Bacillus] caldotenax Species 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 235000004279 alanine Nutrition 0.000 description 1
- 238000012867 alanine scanning Methods 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000007248 cellular mechanism Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000000749 co-immunoprecipitation Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 210000004292 cytoskeleton Anatomy 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000001493 electron microscopy Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 229940009493 gel-one Drugs 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 229940037467 helicobacter pylori Drugs 0.000 description 1
- 102000044255 human PCNA Human genes 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 150000003833 nucleoside derivatives Chemical class 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 238000002823 phage display Methods 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 239000013615 primer Substances 0.000 description 1
- 239000002987 primer (paints) Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001915 proofreading effect Effects 0.000 description 1
- 230000006916 protein interaction Effects 0.000 description 1
- 230000004850 protein–protein interaction Effects 0.000 description 1
- 230000002797 proteolythic effect Effects 0.000 description 1
- 239000011535 reaction buffer Substances 0.000 description 1
- 238000003259 recombinant expression Methods 0.000 description 1
- 230000006798 recombination Effects 0.000 description 1
- 238000005215 recombination Methods 0.000 description 1
- 239000002342 ribonucleoside Substances 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 108060008226 thioredoxin Proteins 0.000 description 1
- 229940094937 thioredoxin Drugs 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19937230.6 | 1999-08-06 | ||
DE1999137230 DE19937230A1 (de) | 1999-08-06 | 1999-08-06 | Chimäre Proteine |
PCT/DE2000/002657 WO2001011051A2 (de) | 1999-08-06 | 2000-08-07 | Chimäre proteine |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2379165A1 true CA2379165A1 (en) | 2001-02-15 |
Family
ID=7917496
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002379165A Abandoned CA2379165A1 (en) | 1999-08-06 | 2000-08-07 | Chimeric proteins |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1198571A2 (de) |
JP (1) | JP2003506089A (de) |
AU (1) | AU6983000A (de) |
CA (1) | CA2379165A1 (de) |
DE (2) | DE19937230A1 (de) |
WO (1) | WO2001011051A2 (de) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7384739B2 (en) | 2001-11-14 | 2008-06-10 | Toyo Boseki Kabushiki Kaisha | Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof |
US8232078B2 (en) | 2001-11-28 | 2012-07-31 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
WO2023059361A1 (en) * | 2021-10-06 | 2023-04-13 | 5Prime Biosciences, Inc. | Polymerases for mixed aqueous-organic media and uses thereof |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP4722035B2 (ja) * | 2003-03-25 | 2011-07-13 | アジレント・テクノロジーズ・インク | Dnaポリメラーゼ融合物およびその使用 |
US20190055527A1 (en) * | 2015-11-27 | 2019-02-21 | Kyushu University, National University Corporation | Dna polymerase variant |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2734567B1 (fr) * | 1995-05-26 | 1997-08-08 | Commissariat Energie Atomique | Proteines chimeriques activant la transcription par la polymerase iii, leur utilisation pour la detection et l'analyse des interactions entre proteines, et genes codant pour lesdites proteines |
AU4159697A (en) * | 1996-08-23 | 1998-03-06 | President And Fellows Of Harvard College | Interaction trap assay, reagents and uses thereof |
WO1998045452A2 (en) * | 1997-04-08 | 1998-10-15 | The Rockfeller University | Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof |
US6238905B1 (en) * | 1997-09-12 | 2001-05-29 | University Technology Corporation | Thermophilic polymerase III holoenzyme |
WO1999037661A1 (en) * | 1998-01-27 | 1999-07-29 | The Rockefeller University | Dna replication proteins of gram positive bacteria and their use to screen for chemical inhibitors |
DE19840771A1 (de) * | 1998-08-06 | 2000-02-10 | Lion Bioscience Ag | Thermostabiler in vitro-Komplex mit Polymeraseaktivität |
WO2000008164A2 (de) * | 1998-08-06 | 2000-02-17 | Lion Bioscience Ag | Thermostabiler in vitro-komplex mit polymeraseaktivität |
-
1999
- 1999-08-06 DE DE1999137230 patent/DE19937230A1/de not_active Withdrawn
-
2000
- 2000-08-07 CA CA002379165A patent/CA2379165A1/en not_active Abandoned
- 2000-08-07 DE DE10082298T patent/DE10082298D2/de not_active Expired - Lifetime
- 2000-08-07 WO PCT/DE2000/002657 patent/WO2001011051A2/de not_active Application Discontinuation
- 2000-08-07 AU AU69830/00A patent/AU6983000A/en not_active Abandoned
- 2000-08-07 JP JP2001515836A patent/JP2003506089A/ja active Pending
- 2000-08-07 EP EP00958230A patent/EP1198571A2/de not_active Withdrawn
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7384739B2 (en) | 2001-11-14 | 2008-06-10 | Toyo Boseki Kabushiki Kaisha | Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof |
US8232078B2 (en) | 2001-11-28 | 2012-07-31 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
US8476045B2 (en) | 2001-11-28 | 2013-07-02 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
US9139873B2 (en) | 2001-11-28 | 2015-09-22 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
US9708598B2 (en) | 2001-11-28 | 2017-07-18 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
WO2023059361A1 (en) * | 2021-10-06 | 2023-04-13 | 5Prime Biosciences, Inc. | Polymerases for mixed aqueous-organic media and uses thereof |
Also Published As
Publication number | Publication date |
---|---|
DE10082298D2 (de) | 2001-11-08 |
AU6983000A (en) | 2001-03-05 |
DE19937230A1 (de) | 2001-02-08 |
EP1198571A2 (de) | 2002-04-24 |
WO2001011051A2 (de) | 2001-02-15 |
JP2003506089A (ja) | 2003-02-18 |
WO2001011051A3 (de) | 2001-06-21 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US6607883B1 (en) | Polymerase chimeras | |
EP1350841B1 (de) | Thermostabile oder thermoactive DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität | |
EP0624641B1 (de) | Thermostable Nukleinsäure Polymerase | |
US8927699B2 (en) | Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications to the same | |
EP1836319B1 (de) | Polypeptide mit nukleinsäurebindender aktivität sowie verfahren zur nukleinsäureamplifikation | |
US5700672A (en) | Purified thermostable pyrococcus furiousus DNA ligase | |
US8637288B2 (en) | Thermostable chimeric nucleic acid polymerases and uses thereof | |
JPH06504196A (ja) | モルモシポ・アフリカヌスからの純化された熱安定性核酸ポリメラーゼ | |
JPH09506783A (ja) | テルモトガ・ネアポリタナからクローニングされたdnaポリメラーゼおよびその変異体 | |
WO1998035060A9 (en) | Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof | |
JPH11501801A (ja) | 耐熱性が向上し、かつ、プライマーエクステンションの長さと効率が向上したdnaポリメラーゼ | |
US20100167292A1 (en) | High fidelity polymerases and uses thereof | |
WO1998035060A1 (en) | Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof | |
US20040002076A1 (en) | Methods of using improved polymerases | |
CA2415767A1 (en) | High fidelity polymerases and uses thereof | |
WO2003066804A2 (en) | Thermus scotoductus nucleic acid polymerases | |
CA2379165A1 (en) | Chimeric proteins | |
JP2002522042A (ja) | ポリメラーゼ活性を有する熱安定性invitro複合体 | |
Zhou | Structural study of Sulfolobus solfataricus DinB lesion bypass DNA polymerase |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |