WO2001011051A3 - Chimäre proteine - Google Patents

Chimäre proteine Download PDF

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Publication number
WO2001011051A3
WO2001011051A3 PCT/DE2000/002657 DE0002657W WO0111051A3 WO 2001011051 A3 WO2001011051 A3 WO 2001011051A3 DE 0002657 W DE0002657 W DE 0002657W WO 0111051 A3 WO0111051 A3 WO 0111051A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
acid synthesis
complex
protein
synthesis activity
Prior art date
Application number
PCT/DE2000/002657
Other languages
English (en)
French (fr)
Other versions
WO2001011051A2 (de
Inventor
Christian Kilger
Michael Motz
Eva Loeser
Manfred Koegl
Original Assignee
Lion Bioscience Ag
Christian Kilger
Michael Motz
Eva Loeser
Manfred Koegl
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lion Bioscience Ag, Christian Kilger, Michael Motz, Eva Loeser, Manfred Koegl filed Critical Lion Bioscience Ag
Priority to JP2001515836A priority Critical patent/JP2003506089A/ja
Priority to AU69830/00A priority patent/AU6983000A/en
Priority to DE10082298T priority patent/DE10082298D2/de
Priority to CA002379165A priority patent/CA2379165A1/en
Priority to EP00958230A priority patent/EP1198571A2/de
Publication of WO2001011051A2 publication Critical patent/WO2001011051A2/de
Publication of WO2001011051A3 publication Critical patent/WO2001011051A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Die Erfindung betrifft ein rekombinantes chimäres Protein, das a) eine erste Domäne mit Nukleinsäuresyntheseaktivität, und b) eine Wechselwirkung vermittelnde Sequenz umfaßt, wobei durch die Wechselwirkung vermittelnde Sequenz ein Komplex aus Nukleinsäuresyntheseaktivität und Gleitklammerprotein gebildet wird, der Komplex verschieden ist von dem Komplex, den die Nukleinsäuresyntheseaktivität und/or das Gleitklammerprotein mit ihren natürlichen Wechselwirkungspartner (n) ausbildet/ausbilden.
PCT/DE2000/002657 1999-08-06 2000-08-07 Chimäre proteine WO2001011051A2 (de)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2001515836A JP2003506089A (ja) 1999-08-06 2000-08-07 キメラタンパク質
AU69830/00A AU6983000A (en) 1999-08-06 2000-08-07 Chimeric proteins
DE10082298T DE10082298D2 (de) 1999-08-06 2000-08-07 Chimäre Proteine
CA002379165A CA2379165A1 (en) 1999-08-06 2000-08-07 Chimeric proteins
EP00958230A EP1198571A2 (de) 1999-08-06 2000-08-07 Chimäre proteine

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE1999137230 DE19937230A1 (de) 1999-08-06 1999-08-06 Chimäre Proteine
DE19937230.6 1999-08-06

Publications (2)

Publication Number Publication Date
WO2001011051A2 WO2001011051A2 (de) 2001-02-15
WO2001011051A3 true WO2001011051A3 (de) 2001-06-21

Family

ID=7917496

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2000/002657 WO2001011051A2 (de) 1999-08-06 2000-08-07 Chimäre proteine

Country Status (6)

Country Link
EP (1) EP1198571A2 (de)
JP (1) JP2003506089A (de)
AU (1) AU6983000A (de)
CA (1) CA2379165A1 (de)
DE (2) DE19937230A1 (de)
WO (1) WO2001011051A2 (de)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1452593B1 (de) * 2001-11-14 2009-04-08 Toyo Boseki Kabushiki Kaisha Dna-synthesepromotoren, mit dna-polymerase assoziierte faktoren und nutzung davon
ATE406456T1 (de) * 2001-11-28 2008-09-15 Bio Rad Laboratories Verfahren zur verwendung verbesserter polymerasen
US7704712B2 (en) 2003-03-25 2010-04-27 Stratagene California DNA polymerase fusions and uses thereof
CN108350087A (zh) * 2015-11-27 2018-07-31 国立大学法人九州大学 Dna聚合酶变体
WO2023059361A1 (en) * 2021-10-06 2023-04-13 5Prime Biosciences, Inc. Polymerases for mixed aqueous-organic media and uses thereof

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996037618A1 (fr) * 1995-05-26 1996-11-28 Commissariat A L'energie Atomique - C.E.A. Proteines chimeriques activant la transcription par la polymerase iii, leur utilisation pour la detection et l'analyse des interactions entre proteines, et genes codant pour lesdites proteines
WO1998007845A1 (en) * 1996-08-23 1998-02-26 President And Fellows Of Harvard College Interaction trap assay, reagents and uses thereof
WO1998045452A2 (en) * 1997-04-08 1998-10-15 The Rockfeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
WO1999013060A1 (en) * 1997-09-12 1999-03-18 Enzyco, Inc. Novel thermophilic polymerase iii holoenzyme
WO1999037661A1 (en) * 1998-01-27 1999-07-29 The Rockefeller University Dna replication proteins of gram positive bacteria and their use to screen for chemical inhibitors
DE19840771A1 (de) * 1998-08-06 2000-02-10 Lion Bioscience Ag Thermostabiler in vitro-Komplex mit Polymeraseaktivität
WO2000008164A2 (de) * 1998-08-06 2000-02-17 Lion Bioscience Ag Thermostabiler in vitro-komplex mit polymeraseaktivität

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996037618A1 (fr) * 1995-05-26 1996-11-28 Commissariat A L'energie Atomique - C.E.A. Proteines chimeriques activant la transcription par la polymerase iii, leur utilisation pour la detection et l'analyse des interactions entre proteines, et genes codant pour lesdites proteines
WO1998007845A1 (en) * 1996-08-23 1998-02-26 President And Fellows Of Harvard College Interaction trap assay, reagents and uses thereof
WO1998045452A2 (en) * 1997-04-08 1998-10-15 The Rockfeller University Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
WO1999013060A1 (en) * 1997-09-12 1999-03-18 Enzyco, Inc. Novel thermophilic polymerase iii holoenzyme
WO1999037661A1 (en) * 1998-01-27 1999-07-29 The Rockefeller University Dna replication proteins of gram positive bacteria and their use to screen for chemical inhibitors
DE19840771A1 (de) * 1998-08-06 2000-02-10 Lion Bioscience Ag Thermostabiler in vitro-Komplex mit Polymeraseaktivität
WO2000008164A2 (de) * 1998-08-06 2000-02-17 Lion Bioscience Ag Thermostabiler in vitro-komplex mit polymeraseaktivität

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
OUHAMMOUCH MOHAMED ET AL: "Activation of RNA polymerase II by topologically linked DNA-tracking proteins.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 94, no. 13, 1997, 1997, pages 6718 - 6723, XP002157966, ISSN: 0027-8424 *
PODUST VLADIMIR N ET AL: "Replication factor C disengages from proliferating cell nuclear antigen (PCNA) upon sliding clamp formation, and PCNA itself tethers DNA polymerase delta to DNA.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 48, 27 November 1998 (1998-11-27), pages 31992 - 31999, XP002157967, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2001011051A2 (de) 2001-02-15
AU6983000A (en) 2001-03-05
CA2379165A1 (en) 2001-02-15
EP1198571A2 (de) 2002-04-24
DE19937230A1 (de) 2001-02-08
JP2003506089A (ja) 2003-02-18
DE10082298D2 (de) 2001-11-08

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