CA2379165A1 - Proteines chimeres - Google Patents

Proteines chimeres Download PDF

Info

Publication number
CA2379165A1
CA2379165A1 CA002379165A CA2379165A CA2379165A1 CA 2379165 A1 CA2379165 A1 CA 2379165A1 CA 002379165 A CA002379165 A CA 002379165A CA 2379165 A CA2379165 A CA 2379165A CA 2379165 A1 CA2379165 A1 CA 2379165A1
Authority
CA
Canada
Prior art keywords
protein
interaction
nucleic acid
sequence
chimeric protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002379165A
Other languages
English (en)
Inventor
Christian Kilger
Michael Motz
Eva Loser
Manfred Kogl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sygnis Pharma AG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2379165A1 publication Critical patent/CA2379165A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne une protéine chimère recombinante comportant a) un premier domaine présentant une activité de synthèse d'acides nucléiques, et b) une séquence établissant une interaction. Cette séquence établissant une interaction permet de former un complexe par l'intermédiaire de l'activité de synthèse d'acides nucléiques et d'une protéine d'attache coulissante, ce complexe étant différent du complexe formé par l'intermédiaire de l'activité de synthèse d'acides nucléiques et/ou de la protéine d'attache coulissante et de leurs partenaires d'interaction naturels.
CA002379165A 1999-08-06 2000-08-07 Proteines chimeres Abandoned CA2379165A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE1999137230 DE19937230A1 (de) 1999-08-06 1999-08-06 Chimäre Proteine
DE19937230.6 1999-08-06
PCT/DE2000/002657 WO2001011051A2 (fr) 1999-08-06 2000-08-07 Proteines chimeres

Publications (1)

Publication Number Publication Date
CA2379165A1 true CA2379165A1 (fr) 2001-02-15

Family

ID=7917496

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002379165A Abandoned CA2379165A1 (fr) 1999-08-06 2000-08-07 Proteines chimeres

Country Status (6)

Country Link
EP (1) EP1198571A2 (fr)
JP (1) JP2003506089A (fr)
AU (1) AU6983000A (fr)
CA (1) CA2379165A1 (fr)
DE (2) DE19937230A1 (fr)
WO (1) WO2001011051A2 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7384739B2 (en) 2001-11-14 2008-06-10 Toyo Boseki Kabushiki Kaisha Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof
US8232078B2 (en) 2001-11-28 2012-07-31 Bio-Rad Laboratories, Inc. Methods of using improved polymerases
WO2023059361A1 (fr) * 2021-10-06 2023-04-13 5Prime Biosciences, Inc. Polymérases pour milieux mixtes aqueux-organiques et leurs utilisations

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602004025801D1 (de) 2003-03-25 2010-04-15 Stratagene California Dna-polymerasefusionen und deren verwendungen
WO2017090685A1 (fr) * 2015-11-27 2017-06-01 国立大学法人九州大学 Mutant d'adn polymérase

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2734567B1 (fr) * 1995-05-26 1997-08-08 Commissariat Energie Atomique Proteines chimeriques activant la transcription par la polymerase iii, leur utilisation pour la detection et l'analyse des interactions entre proteines, et genes codant pour lesdites proteines
AU4159697A (en) * 1996-08-23 1998-03-06 President And Fellows Of Harvard College Interaction trap assay, reagents and uses thereof
AU7683998A (en) * 1997-04-08 1998-10-30 Rockfeller University, The Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
US6238905B1 (en) * 1997-09-12 2001-05-29 University Technology Corporation Thermophilic polymerase III holoenzyme
CA2318574A1 (fr) * 1998-01-27 1999-07-29 The Rockefeller University Proteines de replication d'adn de bacteries gram-positif et leur utilisation dans le criblage d'inhibiteurs chimiques
WO2000008164A2 (fr) * 1998-08-06 2000-02-17 Lion Bioscience Ag Complexe in vitro thermostable a activite de polymerase
DE19840771A1 (de) * 1998-08-06 2000-02-10 Lion Bioscience Ag Thermostabiler in vitro-Komplex mit Polymeraseaktivität

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7384739B2 (en) 2001-11-14 2008-06-10 Toyo Boseki Kabushiki Kaisha Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof
US8232078B2 (en) 2001-11-28 2012-07-31 Bio-Rad Laboratories, Inc. Methods of using improved polymerases
US8476045B2 (en) 2001-11-28 2013-07-02 Bio-Rad Laboratories, Inc. Methods of using improved polymerases
US9139873B2 (en) 2001-11-28 2015-09-22 Bio-Rad Laboratories, Inc. Methods of using improved polymerases
US9708598B2 (en) 2001-11-28 2017-07-18 Bio-Rad Laboratories, Inc. Methods of using improved polymerases
WO2023059361A1 (fr) * 2021-10-06 2023-04-13 5Prime Biosciences, Inc. Polymérases pour milieux mixtes aqueux-organiques et leurs utilisations

Also Published As

Publication number Publication date
DE19937230A1 (de) 2001-02-08
WO2001011051A3 (fr) 2001-06-21
WO2001011051A2 (fr) 2001-02-15
DE10082298D2 (de) 2001-11-08
EP1198571A2 (fr) 2002-04-24
JP2003506089A (ja) 2003-02-18
AU6983000A (en) 2001-03-05

Similar Documents

Publication Publication Date Title
US6607883B1 (en) Polymerase chimeras
EP1350841B1 (fr) ADN polymérase thermostable ou thermoactif ayant une activité 3' - 5' exonucléase atténuée
EP0624641B1 (fr) Polymérase d'acide nucléique thermostable
US8927699B2 (en) Methods for obtaining thermostable enzymes, DNA polymerase I variants from Thermus aquaticus having new catalytic activities, methods for obtaining the same, and applications to the same
EP1836319B1 (fr) Polypeptides presentant une activite de liaison d'acides nucleiques et procedes permettant d'amplifier des acides nucleiques
US5700672A (en) Purified thermostable pyrococcus furiousus DNA ligase
US8637288B2 (en) Thermostable chimeric nucleic acid polymerases and uses thereof
JPH06504196A (ja) モルモシポ・アフリカヌスからの純化された熱安定性核酸ポリメラーゼ
JPH09506783A (ja) テルモトガ・ネアポリタナからクローニングされたdnaポリメラーゼおよびその変異体
WO1998035060A9 (fr) Polymerases pour l'analyse et le typage de fragments d'acide nucleique polymorphes et leurs utilisations
JPH11501801A (ja) 耐熱性が向上し、かつ、プライマーエクステンションの長さと効率が向上したdnaポリメラーゼ
US20100167292A1 (en) High fidelity polymerases and uses thereof
WO1998035060A1 (fr) Polymerases pour l'analyse et le typage de fragments d'acide nucleique polymorphes et leurs utilisations
US20040002076A1 (en) Methods of using improved polymerases
CA2415767A1 (fr) Polymerases haute fidelite et leur utilisation
EP1466013A2 (fr) Polymerases d'acide nucleique de thermus scotoductus
CA2379165A1 (fr) Proteines chimeres
JP2002522042A (ja) ポリメラーゼ活性を有する熱安定性invitro複合体
Zhou Structural study of Sulfolobus solfataricus DinB lesion bypass DNA polymerase

Legal Events

Date Code Title Description
FZDE Discontinued