CA2379165A1 - Proteines chimeres - Google Patents
Proteines chimeres Download PDFInfo
- Publication number
- CA2379165A1 CA2379165A1 CA002379165A CA2379165A CA2379165A1 CA 2379165 A1 CA2379165 A1 CA 2379165A1 CA 002379165 A CA002379165 A CA 002379165A CA 2379165 A CA2379165 A CA 2379165A CA 2379165 A1 CA2379165 A1 CA 2379165A1
- Authority
- CA
- Canada
- Prior art keywords
- protein
- interaction
- nucleic acid
- sequence
- chimeric protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 102000037865 fusion proteins Human genes 0.000 title claims description 67
- 108020001507 fusion proteins Proteins 0.000 title claims description 67
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 297
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 259
- 230000000694 effects Effects 0.000 claims abstract description 141
- 238000001668 nucleic acid synthesis Methods 0.000 claims abstract description 69
- 230000003993 interaction Effects 0.000 claims abstract description 65
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims abstract description 63
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims abstract description 63
- 150000007523 nucleic acids Chemical class 0.000 claims description 76
- 108020004707 nucleic acids Proteins 0.000 claims description 71
- 102000039446 nucleic acids Human genes 0.000 claims description 71
- 238000000034 method Methods 0.000 claims description 54
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- 101001092206 Homo sapiens Replication protein A 32 kDa subunit Proteins 0.000 description 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
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- 241000204103 Thermococcus fumicolans Species 0.000 description 1
- 241001237851 Thermococcus gorgonarius Species 0.000 description 1
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- 241000206213 Thermosipho africanus Species 0.000 description 1
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- 241000557726 Thermus oshimai Species 0.000 description 1
- 241001522143 Thermus scotoductus Species 0.000 description 1
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- 108091023040 Transcription factor Proteins 0.000 description 1
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- 241000193758 [Bacillus] caldotenax Species 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention concerne une protéine chimère recombinante comportant a) un premier domaine présentant une activité de synthèse d'acides nucléiques, et b) une séquence établissant une interaction. Cette séquence établissant une interaction permet de former un complexe par l'intermédiaire de l'activité de synthèse d'acides nucléiques et d'une protéine d'attache coulissante, ce complexe étant différent du complexe formé par l'intermédiaire de l'activité de synthèse d'acides nucléiques et/ou de la protéine d'attache coulissante et de leurs partenaires d'interaction naturels.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE1999137230 DE19937230A1 (de) | 1999-08-06 | 1999-08-06 | Chimäre Proteine |
DE19937230.6 | 1999-08-06 | ||
PCT/DE2000/002657 WO2001011051A2 (fr) | 1999-08-06 | 2000-08-07 | Proteines chimeres |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2379165A1 true CA2379165A1 (fr) | 2001-02-15 |
Family
ID=7917496
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002379165A Abandoned CA2379165A1 (fr) | 1999-08-06 | 2000-08-07 | Proteines chimeres |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1198571A2 (fr) |
JP (1) | JP2003506089A (fr) |
AU (1) | AU6983000A (fr) |
CA (1) | CA2379165A1 (fr) |
DE (2) | DE19937230A1 (fr) |
WO (1) | WO2001011051A2 (fr) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7384739B2 (en) | 2001-11-14 | 2008-06-10 | Toyo Boseki Kabushiki Kaisha | Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof |
US8232078B2 (en) | 2001-11-28 | 2012-07-31 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
WO2023059361A1 (fr) * | 2021-10-06 | 2023-04-13 | 5Prime Biosciences, Inc. | Polymérases pour milieux mixtes aqueux-organiques et leurs utilisations |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE602004025801D1 (de) | 2003-03-25 | 2010-04-15 | Stratagene California | Dna-polymerasefusionen und deren verwendungen |
WO2017090685A1 (fr) * | 2015-11-27 | 2017-06-01 | 国立大学法人九州大学 | Mutant d'adn polymérase |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2734567B1 (fr) * | 1995-05-26 | 1997-08-08 | Commissariat Energie Atomique | Proteines chimeriques activant la transcription par la polymerase iii, leur utilisation pour la detection et l'analyse des interactions entre proteines, et genes codant pour lesdites proteines |
AU4159697A (en) * | 1996-08-23 | 1998-03-06 | President And Fellows Of Harvard College | Interaction trap assay, reagents and uses thereof |
AU7683998A (en) * | 1997-04-08 | 1998-10-30 | Rockfeller University, The | Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof |
US6238905B1 (en) * | 1997-09-12 | 2001-05-29 | University Technology Corporation | Thermophilic polymerase III holoenzyme |
CA2318574A1 (fr) * | 1998-01-27 | 1999-07-29 | The Rockefeller University | Proteines de replication d'adn de bacteries gram-positif et leur utilisation dans le criblage d'inhibiteurs chimiques |
WO2000008164A2 (fr) * | 1998-08-06 | 2000-02-17 | Lion Bioscience Ag | Complexe in vitro thermostable a activite de polymerase |
DE19840771A1 (de) * | 1998-08-06 | 2000-02-10 | Lion Bioscience Ag | Thermostabiler in vitro-Komplex mit Polymeraseaktivität |
-
1999
- 1999-08-06 DE DE1999137230 patent/DE19937230A1/de not_active Withdrawn
-
2000
- 2000-08-07 DE DE10082298T patent/DE10082298D2/de not_active Expired - Lifetime
- 2000-08-07 WO PCT/DE2000/002657 patent/WO2001011051A2/fr not_active Application Discontinuation
- 2000-08-07 AU AU69830/00A patent/AU6983000A/en not_active Abandoned
- 2000-08-07 JP JP2001515836A patent/JP2003506089A/ja active Pending
- 2000-08-07 EP EP00958230A patent/EP1198571A2/fr not_active Withdrawn
- 2000-08-07 CA CA002379165A patent/CA2379165A1/fr not_active Abandoned
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7384739B2 (en) | 2001-11-14 | 2008-06-10 | Toyo Boseki Kabushiki Kaisha | Compositions for enhancing DNA synthesis, DNA polymerase-related factors and utilization thereof |
US8232078B2 (en) | 2001-11-28 | 2012-07-31 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
US8476045B2 (en) | 2001-11-28 | 2013-07-02 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
US9139873B2 (en) | 2001-11-28 | 2015-09-22 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
US9708598B2 (en) | 2001-11-28 | 2017-07-18 | Bio-Rad Laboratories, Inc. | Methods of using improved polymerases |
WO2023059361A1 (fr) * | 2021-10-06 | 2023-04-13 | 5Prime Biosciences, Inc. | Polymérases pour milieux mixtes aqueux-organiques et leurs utilisations |
Also Published As
Publication number | Publication date |
---|---|
DE19937230A1 (de) | 2001-02-08 |
WO2001011051A3 (fr) | 2001-06-21 |
WO2001011051A2 (fr) | 2001-02-15 |
DE10082298D2 (de) | 2001-11-08 |
EP1198571A2 (fr) | 2002-04-24 |
JP2003506089A (ja) | 2003-02-18 |
AU6983000A (en) | 2001-03-05 |
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