CA2375098A1 - Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires - Google Patents

Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires Download PDF

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Publication number
CA2375098A1
CA2375098A1 CA002375098A CA2375098A CA2375098A1 CA 2375098 A1 CA2375098 A1 CA 2375098A1 CA 002375098 A CA002375098 A CA 002375098A CA 2375098 A CA2375098 A CA 2375098A CA 2375098 A1 CA2375098 A1 CA 2375098A1
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Prior art keywords
recombinant
virus
helper virus
scei
restriction enzyme
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Abandoned
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CA002375098A
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English (en)
Inventor
Weidong Xiao
James M. Wilson
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University of Pennsylvania Penn
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Individual
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Publication of CA2375098A1 publication Critical patent/CA2375098A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10351—Methods of production or purification of viral material
    • C12N2710/10352—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne une méthode de fabrication de virus recombinés nécessitant des virus auxiliaires pour l'encapsidation. La méthode fait intervenir un virus auxiliaire recombiné qui a été conçu pour pouvoir contenir au moins un site de restriction rare (par exemple, I-SceI), un vecteur viral qui dépend de l'auxiliaire pour l'encapsidation, et une cellule hôte recombinée pouvant exprimer un enzyme de restriction rare (par exemple, I-SceI). La méthode consiste à transfecter ou à infecter la cellule hôte avec le virus auxiliaire et le vecteur viral et à incuber la cellule dans des conditions qui permettent l'encapsidation du vecteur viral dans une capside d'adénovirus. Ensuite, le I-SceI exprimé par la cellule hôte digère le virus auxiliaire, ce qui permet une séparation immédiate des fragments digérés du virus auxiliaire d'avec le virus recombiné.
CA002375098A 1999-06-02 2000-01-07 Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires Abandoned CA2375098A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13710599P 1999-06-02 1999-06-02
US60/137,105 1999-06-02
PCT/US2000/000415 WO2000075353A1 (fr) 1999-06-02 2000-01-07 Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires

Publications (1)

Publication Number Publication Date
CA2375098A1 true CA2375098A1 (fr) 2000-12-14

Family

ID=22475854

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002375098A Abandoned CA2375098A1 (fr) 1999-06-02 2000-01-07 Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires

Country Status (5)

Country Link
EP (1) EP1183380A1 (fr)
JP (1) JP2003501067A (fr)
AU (1) AU2409200A (fr)
CA (1) CA2375098A1 (fr)
WO (1) WO2000075353A1 (fr)

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Publication number Publication date
EP1183380A1 (fr) 2002-03-06
JP2003501067A (ja) 2003-01-14
AU2409200A (en) 2000-12-28
WO2000075353A1 (fr) 2000-12-14

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