CA2375098A1 - Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires - Google Patents
Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires Download PDFInfo
- Publication number
- CA2375098A1 CA2375098A1 CA002375098A CA2375098A CA2375098A1 CA 2375098 A1 CA2375098 A1 CA 2375098A1 CA 002375098 A CA002375098 A CA 002375098A CA 2375098 A CA2375098 A CA 2375098A CA 2375098 A1 CA2375098 A1 CA 2375098A1
- Authority
- CA
- Canada
- Prior art keywords
- recombinant
- virus
- helper virus
- scei
- restriction enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10341—Use of virus, viral particle or viral elements as a vector
- C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10351—Methods of production or purification of viral material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/10011—Adenoviridae
- C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
- C12N2710/10351—Methods of production or purification of viral material
- C12N2710/10352—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
L'invention concerne une méthode de fabrication de virus recombinés nécessitant des virus auxiliaires pour l'encapsidation. La méthode fait intervenir un virus auxiliaire recombiné qui a été conçu pour pouvoir contenir au moins un site de restriction rare (par exemple, I-SceI), un vecteur viral qui dépend de l'auxiliaire pour l'encapsidation, et une cellule hôte recombinée pouvant exprimer un enzyme de restriction rare (par exemple, I-SceI). La méthode consiste à transfecter ou à infecter la cellule hôte avec le virus auxiliaire et le vecteur viral et à incuber la cellule dans des conditions qui permettent l'encapsidation du vecteur viral dans une capside d'adénovirus. Ensuite, le I-SceI exprimé par la cellule hôte digère le virus auxiliaire, ce qui permet une séparation immédiate des fragments digérés du virus auxiliaire d'avec le virus recombiné.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US13710599P | 1999-06-02 | 1999-06-02 | |
US60/137,105 | 1999-06-02 | ||
PCT/US2000/000415 WO2000075353A1 (fr) | 1999-06-02 | 2000-01-07 | Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2375098A1 true CA2375098A1 (fr) | 2000-12-14 |
Family
ID=22475854
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002375098A Abandoned CA2375098A1 (fr) | 1999-06-02 | 2000-01-07 | Compositions et methodes pour la fabrication de virus recombines necessitant des virus auxiliaires |
Country Status (5)
Country | Link |
---|---|
EP (1) | EP1183380A1 (fr) |
JP (1) | JP2003501067A (fr) |
AU (1) | AU2409200A (fr) |
CA (1) | CA2375098A1 (fr) |
WO (1) | WO2000075353A1 (fr) |
Families Citing this family (80)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6759237B1 (en) | 1998-11-05 | 2004-07-06 | The Trustees Of The University Of Pennsylvania | Adeno-associated virus serotype 1 nucleic acid sequences, vectors and host cells containing same |
US7094595B2 (en) | 2000-10-30 | 2006-08-22 | Sru Biosystems, Inc. | Label-free high-throughput optical technique for detecting biomolecular interactions |
PL222683B1 (pl) | 2001-11-13 | 2016-08-31 | Univ Pennsylvania | Rekombinowane wirusy stowarzyszone z adenowirusem (AAV), sposoby ich wytwarzania, pakująca komórka gospodarza, kompozycja zawierająca wirus, zastosowanie wirusa, wyizolowane wirusy AAV, białka,sztuczne białka kapsydu, cząsteczki, komórki gospodarza, sposoby dostarczania transgenu, sposób identyfikacji serotypu, zestaw diagnostyczny, sposób izolacji nowych wirusów, rekombinowana komórka |
EP1463805B1 (fr) | 2001-12-17 | 2014-10-22 | The Trustees of The University of Pennsylvania | Sequences du serotype 9 du virus associe a l'adenovirus (aav), vecteurs les contenant et utilisations correspondantes |
PT1453547T (pt) | 2001-12-17 | 2016-12-28 | Univ Pennsylvania | Sequências do vírus adeno-associado (aav) do serotipo 8, vetores contendo as mesmas, e utilizações destas |
EP1359217B1 (fr) | 2002-04-29 | 2006-12-13 | The Trustees of The University of Pennsylvania | Méthode pour l'amplification et production des virus intégrés dans l'ADN cellulaire de tissu |
PL3116900T3 (pl) | 2014-03-09 | 2021-03-08 | The Trustees Of The University Of Pennsylvania | Kompozycje użyteczne w leczeniu niedoboru transkarbamylazy ornitynowej (otc) |
WO2015191508A1 (fr) | 2014-06-09 | 2015-12-17 | Voyager Therapeutics, Inc. | Capsides chimériques |
BR112017009497A2 (pt) | 2014-11-05 | 2018-02-06 | Voyager Therapeutics, Inc. | polinucleotídeos de aadc para o tratamento da doença de parkinson |
ES2878451T3 (es) | 2014-11-14 | 2021-11-18 | Voyager Therapeutics Inc | Polinucleótidos moduladores |
US10597660B2 (en) | 2014-11-14 | 2020-03-24 | Voyager Therapeutics, Inc. | Compositions and methods of treating amyotrophic lateral sclerosis (ALS) |
EP3230441A4 (fr) | 2014-12-12 | 2018-10-03 | Voyager Therapeutics, Inc. | Compositions et méthodes pour la production de scaav |
US20180230489A1 (en) | 2015-10-28 | 2018-08-16 | Voyager Therapeutics, Inc. | Regulatable expression using adeno-associated virus (aav) |
CA3006569A1 (fr) | 2015-12-02 | 2017-06-08 | Voyager Therapeutics, Inc. | Dosages pour la detection d'anticorps neutralisants de vaa |
WO2017189964A2 (fr) | 2016-04-29 | 2017-11-02 | Voyager Therapeutics, Inc. | Compositions pour le traitement de maladies |
US11326182B2 (en) | 2016-04-29 | 2022-05-10 | Voyager Therapeutics, Inc. | Compositions for the treatment of disease |
US11951121B2 (en) | 2016-05-18 | 2024-04-09 | Voyager Therapeutics, Inc. | Compositions and methods for treating Huntington's disease |
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CA3035522A1 (fr) | 2016-08-30 | 2018-03-08 | The Regents Of The University Of California | Procedes de ciblage et d'administration biomedicaux, et dispositifs et systemes pour la mise en uvre de ceux-ci |
CN111108198A (zh) | 2017-05-05 | 2020-05-05 | 沃雅戈治疗公司 | 治疗亨廷顿病的组合物和方法 |
CA3061652A1 (fr) | 2017-05-05 | 2018-11-08 | Voyager Therapeutics, Inc. | Compositions et methodes de traitement de la sclerose laterale amyotrophique (sla) |
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AU2018302016A1 (en) | 2017-07-17 | 2020-02-06 | The Regents Of The University Of California | Trajectory array guide system |
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GB202201713D0 (en) | 2022-02-10 | 2022-03-30 | Univ Dundee | An affinity directed phosphatase system for targeted protein dephosphorylation |
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WO2024006741A1 (fr) | 2022-06-28 | 2024-01-04 | Voyager Therapeutics, Inc. | Variants de capside d'aav et leurs utilisations |
WO2024011112A1 (fr) | 2022-07-06 | 2024-01-11 | Voyager Therapeutics, Inc. | Variants de capside d'aav et leurs utilisations |
WO2024054983A1 (fr) | 2022-09-08 | 2024-03-14 | Voyager Therapeutics, Inc. | Expression controlée de protéines virales |
TW202428602A (zh) | 2022-09-15 | 2024-07-16 | 美商航海家醫療公司 | Tau結合化合物 |
WO2024064856A1 (fr) | 2022-09-22 | 2024-03-28 | Biomarin Pharmaceutical Inc. | Traitement de la cardiomyopathie au moyen de vecteurs de thérapie génique aav |
TW202421788A (zh) | 2022-09-22 | 2024-06-01 | 美商拜奧馬林製藥公司 | 用aav基因療法載體治療致心律不整性心肌病 |
WO2024145474A2 (fr) | 2022-12-29 | 2024-07-04 | Voyager Therapeutics, Inc. | Compositions et procédés de régulation de mapt |
CN117925663A (zh) * | 2023-12-04 | 2024-04-26 | 广州派真生物技术有限公司 | 一种用于重组腺相关病毒包装的质粒系统及其应用 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5792632A (en) * | 1992-05-05 | 1998-08-11 | Institut Pasteur | Nucleotide sequence encoding the enzyme I-SceI and the uses thereof |
AU680459B2 (en) * | 1992-12-03 | 1997-07-31 | Genzyme Corporation | Gene therapy for cystic fibrosis |
EP0716148B1 (fr) * | 1993-09-15 | 2004-01-02 | Chiron Corporation | Vecteurs composés d'alphavirus recombinants |
-
2000
- 2000-01-07 JP JP2001501635A patent/JP2003501067A/ja active Pending
- 2000-01-07 CA CA002375098A patent/CA2375098A1/fr not_active Abandoned
- 2000-01-07 EP EP00902362A patent/EP1183380A1/fr not_active Withdrawn
- 2000-01-07 AU AU24092/00A patent/AU2409200A/en not_active Abandoned
- 2000-01-07 WO PCT/US2000/000415 patent/WO2000075353A1/fr not_active Application Discontinuation
Also Published As
Publication number | Publication date |
---|---|
EP1183380A1 (fr) | 2002-03-06 |
JP2003501067A (ja) | 2003-01-14 |
AU2409200A (en) | 2000-12-28 |
WO2000075353A1 (fr) | 2000-12-14 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |