CA2331335A1 - Rearrangement de genes modifies par codon - Google Patents

Rearrangement de genes modifies par codon Download PDF

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Publication number
CA2331335A1
CA2331335A1 CA002331335A CA2331335A CA2331335A1 CA 2331335 A1 CA2331335 A1 CA 2331335A1 CA 002331335 A CA002331335 A CA 002331335A CA 2331335 A CA2331335 A CA 2331335A CA 2331335 A1 CA2331335 A1 CA 2331335A1
Authority
CA
Canada
Prior art keywords
nucleic acid
codon
nucleic acids
library
codon altered
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002331335A
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English (en)
Inventor
Phillip A. Patten
Lu Liu
Willem P. C. Stemmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Maxygen Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2331335A1 publication Critical patent/CA2331335A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00689—Automatic using computers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

La présente invention concerne des techniques de recombinaison de banques, modifiées par codon, d'acides nucléiques. En plus des modifications par codon, ces acides nucléiques peuvent inclure des modifications conservatrices ou non conservatrices des séquences de codage, par opposition à des séquences de type sauvage. L'invention concerne l'obtention, outre de nouvelles protéines, de vecteurs caractérisés par des taux réduits de retransformation en virus de type sauvage et atténué.
CA002331335A 1998-09-29 1999-09-28 Rearrangement de genes modifies par codon Abandoned CA2331335A1 (fr)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US10236298P 1998-09-29 1998-09-29
US60/102,362 1998-09-29
US11772999P 1999-01-29 1999-01-29
US60/117,729 1999-01-29
US11881399P 1999-02-05 1999-02-05
US60/118,813 1999-02-05
US14104999P 1999-06-24 1999-06-24
US60/141,049 1999-06-24
PCT/US1999/022588 WO2000018906A2 (fr) 1998-09-29 1999-09-28 Rearrangement de genes modifies par codon

Publications (1)

Publication Number Publication Date
CA2331335A1 true CA2331335A1 (fr) 2000-04-06

Family

ID=27493256

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002331335A Abandoned CA2331335A1 (fr) 1998-09-29 1999-09-28 Rearrangement de genes modifies par codon

Country Status (7)

Country Link
EP (1) EP1117777A2 (fr)
JP (1) JP2002537758A (fr)
KR (1) KR20010085850A (fr)
AU (1) AU1199000A (fr)
CA (1) CA2331335A1 (fr)
IL (1) IL140441A0 (fr)
WO (1) WO2000018906A2 (fr)

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Also Published As

Publication number Publication date
EP1117777A2 (fr) 2001-07-25
WO2000018906A3 (fr) 2000-10-26
KR20010085850A (ko) 2001-09-07
JP2002537758A (ja) 2002-11-12
IL140441A0 (en) 2002-02-10
WO2000018906A9 (fr) 2000-08-31
WO2000018906A2 (fr) 2000-04-06
AU1199000A (en) 2000-04-17

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Effective date: 20040928