WO2000018906A3 - Rearrangement de genes modifies par codon - Google Patents

Rearrangement de genes modifies par codon Download PDF

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Publication number
WO2000018906A3
WO2000018906A3 PCT/US1999/022588 US9922588W WO0018906A3 WO 2000018906 A3 WO2000018906 A3 WO 2000018906A3 US 9922588 W US9922588 W US 9922588W WO 0018906 A3 WO0018906 A3 WO 0018906A3
Authority
WO
WIPO (PCT)
Prior art keywords
shuffling
codon
altered genes
wild
codon altered
Prior art date
Application number
PCT/US1999/022588
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English (en)
Other versions
WO2000018906A9 (fr
WO2000018906A2 (fr
Inventor
Phillip A Patten
Lu Liu
Willem P C Stemmer
Original Assignee
Maxygen Inc
Phillip A Patten
Lu Liu
Willem P C Stemmer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Maxygen Inc, Phillip A Patten, Lu Liu, Willem P C Stemmer filed Critical Maxygen Inc
Priority to KR1020017003873A priority Critical patent/KR20010085850A/ko
Priority to CA002331335A priority patent/CA2331335A1/fr
Priority to EP99969739A priority patent/EP1117777A2/fr
Priority to JP2000572353A priority patent/JP2002537758A/ja
Priority to AU11990/00A priority patent/AU1199000A/en
Priority to IL14044199A priority patent/IL140441A0/xx
Publication of WO2000018906A2 publication Critical patent/WO2000018906A2/fr
Publication of WO2000018906A9 publication Critical patent/WO2000018906A9/fr
Publication of WO2000018906A3 publication Critical patent/WO2000018906A3/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/53—Colony-stimulating factor [CSF]
    • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/0068—Means for controlling the apparatus of the process
    • B01J2219/00686—Automatic
    • B01J2219/00689—Automatic using computers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16111—Human Immunodeficiency Virus, HIV concerning HIV env
    • C12N2740/16122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La présente invention concerne des techniques de recombinaison de banques, modifiées par codon, d'acides nucléiques. En plus des modifications par codon, ces acides nucléiques peuvent inclure des modifications conservatrices ou non conservatrices des séquences de codage, par opposition à des séquences de type sauvage. L'invention concerne l'obtention, outre de nouvelles protéines, de vecteurs caractérisés par des taux réduits de retransformation en virus de type sauvage et atténué.
PCT/US1999/022588 1998-09-29 1999-09-28 Rearrangement de genes modifies par codon WO2000018906A2 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
KR1020017003873A KR20010085850A (ko) 1998-09-29 1999-09-28 코돈 변형 유전자의 재편성
CA002331335A CA2331335A1 (fr) 1998-09-29 1999-09-28 Rearrangement de genes modifies par codon
EP99969739A EP1117777A2 (fr) 1998-09-29 1999-09-28 Rearrangement de genes modifies par codon
JP2000572353A JP2002537758A (ja) 1998-09-29 1999-09-28 コドン変更された遺伝子のシャッフリング
AU11990/00A AU1199000A (en) 1998-09-29 1999-09-28 Shuffling of codon altered genes
IL14044199A IL140441A0 (en) 1998-09-29 1999-09-28 Shuffling of codon altered genes

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US10236298P 1998-09-29 1998-09-29
US11772999P 1999-01-29 1999-01-29
US11881399P 1999-02-05 1999-02-05
US60/118,813 1999-02-05
US14104999P 1999-06-24 1999-06-24
US60/141,049 1999-06-24
US60/102,362 1999-06-24
US60/117,729 1999-06-24

Publications (3)

Publication Number Publication Date
WO2000018906A2 WO2000018906A2 (fr) 2000-04-06
WO2000018906A9 WO2000018906A9 (fr) 2000-08-31
WO2000018906A3 true WO2000018906A3 (fr) 2000-10-26

Family

ID=27493256

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/022588 WO2000018906A2 (fr) 1998-09-29 1999-09-28 Rearrangement de genes modifies par codon

Country Status (7)

Country Link
EP (1) EP1117777A2 (fr)
JP (1) JP2002537758A (fr)
KR (1) KR20010085850A (fr)
AU (1) AU1199000A (fr)
CA (1) CA2331335A1 (fr)
IL (1) IL140441A0 (fr)
WO (1) WO2000018906A2 (fr)

Cited By (4)

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Publication number Priority date Publication date Assignee Title
US7863030B2 (en) 2003-06-17 2011-01-04 The California Institute Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome P450
US8026085B2 (en) 2006-08-04 2011-09-27 California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8076114B2 (en) 2001-07-20 2011-12-13 California Institute Of Technology Cytochrome P450 oxygenases
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules

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US7045289B2 (en) 1991-09-09 2006-05-16 Third Wave Technologies, Inc. Detection of RNA Sequences
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8076114B2 (en) 2001-07-20 2011-12-13 California Institute Of Technology Cytochrome P450 oxygenases
US8367386B2 (en) 2001-07-20 2013-02-05 California Institute Of Technology Cytochrome P450 oxygenases
US8722371B2 (en) 2001-07-20 2014-05-13 California Institute Of Technology Cytochrome P450 oxygenases
US7863030B2 (en) 2003-06-17 2011-01-04 The California Institute Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome P450
US8343744B2 (en) 2003-06-17 2013-01-01 The California Institute Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome P450
US8741616B2 (en) 2003-06-17 2014-06-03 California Institute Of Technology Regio- and enantioselective alkane hydroxylation with modified cytochrome P450
US8026085B2 (en) 2006-08-04 2011-09-27 California Institute Of Technology Methods and systems for selective fluorination of organic molecules
US8252559B2 (en) 2006-08-04 2012-08-28 The California Institute Of Technology Methods and systems for selective fluorination of organic molecules

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WO2000018906A9 (fr) 2000-08-31
IL140441A0 (en) 2002-02-10
CA2331335A1 (fr) 2000-04-06
EP1117777A2 (fr) 2001-07-25
WO2000018906A2 (fr) 2000-04-06
KR20010085850A (ko) 2001-09-07
AU1199000A (en) 2000-04-17
JP2002537758A (ja) 2002-11-12

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