CA2323075A1 - Procede et dispositif de detection d'une sequence nucleotidique - Google Patents

Procede et dispositif de detection d'une sequence nucleotidique Download PDF

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Publication number
CA2323075A1
CA2323075A1 CA002323075A CA2323075A CA2323075A1 CA 2323075 A1 CA2323075 A1 CA 2323075A1 CA 002323075 A CA002323075 A CA 002323075A CA 2323075 A CA2323075 A CA 2323075A CA 2323075 A1 CA2323075 A1 CA 2323075A1
Authority
CA
Canada
Prior art keywords
primer
molecule
fluorophoric
microtiter plate
fluorophoric molecule
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002323075A
Other languages
English (en)
Inventor
Wolf Bertling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
November AG Novus Medicatus Bertling Gesellschaft fuer Molekular Medizin
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2323075A1 publication Critical patent/CA2323075A1/fr
Abandoned legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

Abstract

Procédé de détection d'une séquence nucléotidique au moyen de la fluorescence selon lequel, en présence de la séquence nuléotidique (N) à détecter, une interaction rendue possible par un transfert d'énergie direct ou exempt de rayonnement est produite ou éliminée entre une première molécule (F1) et une seconde molécule (F2). Selon la présente invention, pour éviter les contaminations et améliorer la sensibilité du procédé, au moins l'une des molécules (F1 et F2) est liée à la surface d'une phase solide (M).
CA002323075A 1998-03-18 1999-03-16 Procede et dispositif de detection d'une sequence nucleotidique Abandoned CA2323075A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19811729A DE19811729C2 (de) 1998-03-18 1998-03-18 Verfahren und Vorrichtung zum Nachweis einer Nukleotidsequenz
DE19811729.9 1998-03-18
PCT/DE1999/000725 WO1999047700A1 (fr) 1998-03-18 1999-03-16 Procede et dispositif de detection d'une sequence nucleotidique

Publications (1)

Publication Number Publication Date
CA2323075A1 true CA2323075A1 (fr) 1999-09-23

Family

ID=7861305

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002323075A Abandoned CA2323075A1 (fr) 1998-03-18 1999-03-16 Procede et dispositif de detection d'une sequence nucleotidique

Country Status (5)

Country Link
EP (1) EP1064407A1 (fr)
JP (1) JP2002506654A (fr)
CA (1) CA2323075A1 (fr)
DE (1) DE19811729C2 (fr)
WO (1) WO1999047700A1 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8247179B2 (en) 2002-03-12 2012-08-21 Enzo Life Sciences, Inc. Processes for quantitative or qualitative detection of single-stranded nucleic acids
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
EP3118333A3 (fr) * 2006-12-13 2017-04-26 Luminex Corporation Systèmes et procédés d'analyse multiplex de pcr en temps réel

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19960076C2 (de) * 1999-12-13 2002-12-05 November Ag Molekulare Medizin Verfahren und Vorrichtung zum Nachweis und zur Quantifizierung von Biomolekülen
DE10111420A1 (de) * 2001-03-09 2002-09-12 Gnothis Holding Sa Ecublens Bestimmung von Analyten durch Fluoreszenz-Korrelationsspektroskopie
JP4710283B2 (ja) * 2004-09-08 2011-06-29 富士レビオ株式会社 核酸配列の増幅法および検出法
US9416153B2 (en) 2011-10-11 2016-08-16 Enzo Life Sciences, Inc. Fluorescent dyes
JP7035972B2 (ja) * 2018-11-09 2022-03-15 横河電機株式会社 核酸配列計測用デバイス

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4996143A (en) * 1985-12-23 1991-02-26 Syngene, Inc. Fluorescent stokes shift probes for polynucleotide hybridization
CA2122450C (fr) * 1991-11-01 2004-07-13 Charles Phillip Morris Processus d'amplification en phase solide
EP0601889A2 (fr) * 1992-12-10 1994-06-15 Maine Medical Center Research Institute Sondes d'acides nucléiques
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5654419A (en) * 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
IL124967A (en) * 1995-12-18 2000-07-26 Univ Washington Method for nucleic acid analysis using fluorescence resonance energy transfer
EP0920440B1 (fr) * 1996-02-09 2012-08-22 Cornell Research Foundation, Inc. Detection de differences entre sequences d'acide nucleique faisant appel a la reaction de detection de ligase et a des reseaux adressables
PT912766E (pt) * 1996-06-04 2009-07-16 Univ Utah Res Found Monitorização da hibridização durante a pcr

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8247179B2 (en) 2002-03-12 2012-08-21 Enzo Life Sciences, Inc. Processes for quantitative or qualitative detection of single-stranded nucleic acids
US9068948B2 (en) 2002-03-12 2015-06-30 Enzo Life Sciences, Inc. Processes for detection of nucleic acids
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
US10144957B2 (en) 2002-03-12 2018-12-04 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
EP3118333A3 (fr) * 2006-12-13 2017-04-26 Luminex Corporation Systèmes et procédés d'analyse multiplex de pcr en temps réel
US10253354B2 (en) 2006-12-13 2019-04-09 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time
US11001877B2 (en) 2006-12-13 2021-05-11 Luminex Corporation Systems and methods for multiplex analysis of PCR in real time

Also Published As

Publication number Publication date
JP2002506654A (ja) 2002-03-05
DE19811729A1 (de) 1999-09-23
DE19811729C2 (de) 2000-05-18
WO1999047700A1 (fr) 1999-09-23
EP1064407A1 (fr) 2001-01-03

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