CA2321992A1 - Banana streak virus promoter and detection - Google Patents
Banana streak virus promoter and detection Download PDFInfo
- Publication number
- CA2321992A1 CA2321992A1 CA002321992A CA2321992A CA2321992A1 CA 2321992 A1 CA2321992 A1 CA 2321992A1 CA 002321992 A CA002321992 A CA 002321992A CA 2321992 A CA2321992 A CA 2321992A CA 2321992 A1 CA2321992 A1 CA 2321992A1
- Authority
- CA
- Canada
- Prior art keywords
- plant
- nucleic acid
- sequence
- promoter sequence
- polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Banana Streak Virus (BSV) genome has been cloned and the promoter identified.
Operable linkage of the promoter to a transcribable sequence allows for transgenic expression in plants, including non-graminaceous monocots particularly Musaceae (Musa and Ensete) and graminaceous monocots such as rice and sugar cane. Methods for detection of BSV infection in plants are also provided.
Operable linkage of the promoter to a transcribable sequence allows for transgenic expression in plants, including non-graminaceous monocots particularly Musaceae (Musa and Ensete) and graminaceous monocots such as rice and sugar cane. Methods for detection of BSV infection in plants are also provided.
Claims (20)
1. An isolated polynucleotide consisting of a Banana Streak Virus promoter sequence selected from:
(a) the promoter sequence of a Nigerian isolate of Banana Streak Virus shown in SEQ ID NO. 2;
(b) a promoter sequence of an isolate of Banana Streak Virus, which promoter sequence is an allelic variant of the promoter sequence of SEQ ID NO. 2;
(c) a fragment of (a) or (b) which, when operably linked to a transcribable sequence, promotes transcription of the transcribable sequence in a Musaceae plant cell.
(a) the promoter sequence of a Nigerian isolate of Banana Streak Virus shown in SEQ ID NO. 2;
(b) a promoter sequence of an isolate of Banana Streak Virus, which promoter sequence is an allelic variant of the promoter sequence of SEQ ID NO. 2;
(c) a fragment of (a) or (b) which, when operably linked to a transcribable sequence, promotes transcription of the transcribable sequence in a Musaceae plant cell.
2. An isolated polynucleotide according to claim 1 wherein said promoter sequence is shown in SEQ ID NO. 2.
3. An isolated polynucleotide consisting of a promoter sequence, which promoter sequence:
(i) selectively hybridizes under stringent conditions with a polynucleotide complementary to a polynucleotide which has the nucleotide sequence shown in SEQ ID NO: 2; and (ii) when operably linked to a transcribable sequence promotes transcription of the transcribable sequence in a Musaceae plant cell.
(i) selectively hybridizes under stringent conditions with a polynucleotide complementary to a polynucleotide which has the nucleotide sequence shown in SEQ ID NO: 2; and (ii) when operably linked to a transcribable sequence promotes transcription of the transcribable sequence in a Musaceae plant cell.
4. An isolated polynucleotide according to claim 2 which has a nucleotide sequence found in a strain of Banana Streak Virus.
5. An isolated polynucleotide consisting of a promoter sequence, which promoter sequence is at least 75% identical to the promoter sequence shown in SEQ ID NO: 2 and which when operably linked to a transcribable sequence promotes transcription of the transcribable sequence in a Musaceae plant cell.
6. A nucleic acid construct comprising the promoter sequence of a polynucleotide according to any one of claims 1 to 5 and a non-Banana Streak Virus sequence.
7. A nucleic acid construct according to claim 6 wherein the promoter sequence is operably linked to a transcribable sequence.
8. A nucleic acid vector suitable for transformation of a plant cell and including the promoter sequence of a polynucleotide according to any one of claims 1 to 5.
9. A nucleic acid vector according to claim 8 wherein the promoter sequence is operably linked to a transcribable sequence.
10. A plant cell containing a heterologous polynucleotide, nucleic acid construct or nucleic acid vector according to any one of claims 1 to 9.
11. A plant cell according to claim 10 having a heterologous said polynucleotide or nucleic acid construct within its chromosome.
12. A plant cell according to claim 10 or claim 11 which is comprised in a plant, a plant part or a plant propagule, or an extract or derivative of a plant.
13. A method of producing a cell according to claim 10 or claim 11, the method including incorporating said polynucleotide, nucleic acid construct or nucleic acid vector into the cell by means of transformation.
14. A method according to claim 13 which includes recombining said polynucleotide or said nucleic acid construct with the cell genome nucleic acid such that it is stably incorporated therein.
15. A method according to claim 13 or claim 14 which includes regenerating a plant from one or more transformed cells.
16. A plant comprising a plant cell according to claim 10 or claim 11.
17. A part or propagule of a plant comprising a plant cell according to claim 10 or claim 11.
18. A method of producing a plant, the method including incorporating a polynucleotide, nucleic acid construct or nucleic acid vector according to any of claims 1 to 9 into a plant cell and regenerating a plant from said plant cell.
19. A method according to claim 18 including sexually or asexually propagating or growing off-spring or a descendant of the plant regenerated from said plant cell.
20. Use of a nucleic acid vector according to claim 8 or claim 9 in the production of a transgenic plant.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB9804293.0 | 1998-02-27 | ||
| GBGB9804293.0A GB9804293D0 (en) | 1998-02-27 | 1998-02-27 | Plant virus promoter and detection |
| PCT/GB1999/000599 WO1999043836A1 (en) | 1998-02-27 | 1999-02-26 | Banana streak virus promoter and detection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2321992A1 true CA2321992A1 (en) | 1999-09-02 |
| CA2321992C CA2321992C (en) | 2010-04-13 |
Family
ID=10827774
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2321992A Expired - Fee Related CA2321992C (en) | 1998-02-27 | 1999-02-26 | Banana streak virus promoter and detection |
Country Status (5)
| Country | Link |
|---|---|
| EP (1) | EP1056876A1 (en) |
| AU (1) | AU751495B2 (en) |
| CA (1) | CA2321992C (en) |
| GB (1) | GB9804293D0 (en) |
| WO (1) | WO1999043836A1 (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7598366B2 (en) | 2002-07-17 | 2009-10-06 | Queensland University Of Technology | TaBV transcriptional control elements, chimeric constructs and uses therefor |
| MX2013007087A (en) * | 2010-12-22 | 2013-07-29 | Du Pont | Viral promoter, truncations thereof, and methods of use. |
| CN103243178B (en) * | 2013-05-06 | 2015-11-18 | 陈定虎 | For detecting the primer of banana streak virus, test kit and detection method |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPN328595A0 (en) * | 1995-05-31 | 1995-06-22 | Queensland University Of Technology | Intergenic regions of banana bunchy top virus |
| AUPO759397A0 (en) * | 1997-06-26 | 1997-07-24 | Bureau Of Sugar Experiment Stations | Plant and viral promoters |
-
1998
- 1998-02-27 GB GBGB9804293.0A patent/GB9804293D0/en not_active Ceased
-
1999
- 1999-02-26 CA CA2321992A patent/CA2321992C/en not_active Expired - Fee Related
- 1999-02-26 EP EP99936095A patent/EP1056876A1/en not_active Withdrawn
- 1999-02-26 AU AU32612/99A patent/AU751495B2/en not_active Ceased
- 1999-02-26 WO PCT/GB1999/000599 patent/WO1999043836A1/en not_active Application Discontinuation
Also Published As
| Publication number | Publication date |
|---|---|
| CA2321992C (en) | 2010-04-13 |
| EP1056876A1 (en) | 2000-12-06 |
| AU3261299A (en) | 1999-09-15 |
| GB9804293D0 (en) | 1998-04-22 |
| AU751495B2 (en) | 2002-08-15 |
| WO1999043836A1 (en) | 1999-09-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20170227 |