CA2303790A1 - Caracterisation d'acide nucleique par spectrometrie de masse - Google Patents

Caracterisation d'acide nucleique par spectrometrie de masse Download PDF

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Publication number
CA2303790A1
CA2303790A1 CA002303790A CA2303790A CA2303790A1 CA 2303790 A1 CA2303790 A1 CA 2303790A1 CA 002303790 A CA002303790 A CA 002303790A CA 2303790 A CA2303790 A CA 2303790A CA 2303790 A1 CA2303790 A1 CA 2303790A1
Authority
CA
Canada
Prior art keywords
mass
nucleic acid
fragments
labels
population
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002303790A
Other languages
English (en)
Inventor
Gunter Schmidt
Andrew Hugin Thompson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Xzillion GmbH and Co KG
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB9719638.0A external-priority patent/GB9719638D0/en
Priority claimed from GBGB9725630.9A external-priority patent/GB9725630D0/en
Application filed by Individual filed Critical Individual
Publication of CA2303790A1 publication Critical patent/CA2303790A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Abstract

La présente invention concerne un procédé permettant d'analyser une population de fragments d'acide nucléiques tous marqués par un marqueur de masse. Ce procédé consiste: i.) à ioniser la population; ii.) à trier en sous-populations, en fonction de la masse, la population ionisée dans un spectromètre de masse, chaque sous-population contenant au moins un fragment marqué; iii.) à cliver chaque population de façon à détacher le marqueur de masse associé à chaque fragment marqué; iv.) à évaluer par spectroscopie de masse la masse de chaque marqueur de masse détaché; et v.) à affecter chaque marqueur de masse au fragment qui lui est associé.
CA002303790A 1997-09-15 1998-09-15 Caracterisation d'acide nucleique par spectrometrie de masse Abandoned CA2303790A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GBGB9719638.0A GB9719638D0 (en) 1997-09-15 1997-09-15 High throughput analysis of sanger dna ladders by tandem mass spectrometry
GB9719638.0 1997-09-15
GBGB9725630.9A GB9725630D0 (en) 1997-12-03 1997-12-03 Charecterising nucleic acid
GB9725630.9 1997-12-03
PCT/GB1998/002789 WO1999014362A1 (fr) 1997-09-15 1998-09-15 Caracterisation d'acide nucleique par spectrometrie de masse

Publications (1)

Publication Number Publication Date
CA2303790A1 true CA2303790A1 (fr) 1999-03-25

Family

ID=26312254

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002303790A Abandoned CA2303790A1 (fr) 1997-09-15 1998-09-15 Caracterisation d'acide nucleique par spectrometrie de masse

Country Status (6)

Country Link
EP (1) EP1015632A1 (fr)
JP (1) JP2001516591A (fr)
AU (1) AU746443B2 (fr)
CA (1) CA2303790A1 (fr)
NZ (1) NZ503289A (fr)
WO (1) WO1999014362A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19824280B4 (de) * 1998-05-29 2004-08-19 Bruker Daltonik Gmbh Mutationsanalyse mittels Massenspektrometrie
GB0006141D0 (en) 2000-03-14 2000-05-03 Brax Group Ltd Mass labels
ATE423221T1 (de) * 2000-06-13 2009-03-15 Univ Boston Verwendung von mass-matched nukleotide in der analyse von oligonukleotidmischungen sowie in der hoch-multiplexen nukleinsäuresequenzierung
EP1425586B1 (fr) 2001-09-14 2007-11-21 Electrophoretics Limited Marqueurs de masse
WO2004070352A2 (fr) 2003-01-30 2004-08-19 Applera Corporation Procedes, melanges, kits et compositions relatives a la determination d'analytes
US20050148087A1 (en) 2004-01-05 2005-07-07 Applera Corporation Isobarically labeled analytes and fragment ions derived therefrom

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69433811T2 (de) * 1993-01-07 2005-06-23 Sequenom, Inc., San Diego Dns - sequenzierung durch massenspektronomie
AU687801B2 (en) * 1993-03-19 1998-03-05 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
GB9504598D0 (en) * 1995-03-03 1995-04-26 Imp Cancer Res Tech Method of nucleic acid analysis
EP0868535B9 (fr) * 1996-01-23 2007-05-09 Qiagen Genomics, Inc. Procedes et compositions permettant de determiner la sequence de molecules d'acides nucleiques
US6051378A (en) * 1996-03-04 2000-04-18 Genetrace Systems Inc. Methods of screening nucleic acids using mass spectrometry
AU2217597A (en) * 1996-03-18 1997-10-22 Sequenom, Inc. Dna sequencing by mass spectrometry
US5803916A (en) * 1996-03-19 1998-09-08 Vital-Tech Ltd. Body and joints massage device
GB9620769D0 (en) * 1996-10-04 1996-11-20 Brax Genomics Ltd Nucleic acid sequencing
WO1998026095A1 (fr) * 1996-12-10 1998-06-18 Genetrace Systems Inc. Molecules d'etiquetage massique, non volatiles et liberables
CA2441655A1 (fr) * 1997-01-15 1998-07-23 Xzillion Gmbh & Co Kg Sondes d'hybridation couples a des marqueurs de masse
AU5974498A (en) * 1997-02-18 1998-09-09 Kinetic Limited Control arrangement for vehicle suspension system

Also Published As

Publication number Publication date
NZ503289A (en) 2002-11-26
JP2001516591A (ja) 2001-10-02
AU9088798A (en) 1999-04-05
WO1999014362A1 (fr) 1999-03-25
EP1015632A1 (fr) 2000-07-05
AU746443B2 (en) 2002-05-02

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued