CA2301875A1 - Procedes de preparation d'acides nucleiques pour des analyses de spectrometrie de masse - Google Patents

Procedes de preparation d'acides nucleiques pour des analyses de spectrometrie de masse Download PDF

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Publication number
CA2301875A1
CA2301875A1 CA002301875A CA2301875A CA2301875A1 CA 2301875 A1 CA2301875 A1 CA 2301875A1 CA 002301875 A CA002301875 A CA 002301875A CA 2301875 A CA2301875 A CA 2301875A CA 2301875 A1 CA2301875 A1 CA 2301875A1
Authority
CA
Canada
Prior art keywords
target nucleic
nucleic acid
strand
mass
amplified target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002301875A
Other languages
English (en)
Inventor
Joseph Albert Monforte
Thomas A. Shaler
Yuping Tan
Christopher H. Becker
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sequenom Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/759,993 external-priority patent/US5965363A/en
Application filed by Individual filed Critical Individual
Publication of CA2301875A1 publication Critical patent/CA2301875A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

L'invention a trait à des procédés destinés au criblage d'acides nucléiques pour rechercher des polymorphismes, par une analyse d'acides nucléiques cibles amplifiés, au moyen de techniques de spectrométrie de masse, et à des procédures visant à améliorer la résolution de masse et la précision de masse de ces procédés de détection de polymorphismes.
CA002301875A 1996-09-19 1997-09-19 Procedes de preparation d'acides nucleiques pour des analyses de spectrometrie de masse Abandoned CA2301875A1 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US71558296A 1996-09-19 1996-09-19
US08/715,582 1996-09-19
US3236996P 1996-12-02 1996-12-02
US60/032,369 1996-12-02
US08/759,993 US5965363A (en) 1996-09-19 1996-12-02 Methods of preparing nucleic acids for mass spectrometric analysis
US08/759,993 1996-12-02
PCT/US1997/017101 WO1998012355A1 (fr) 1996-09-19 1997-09-19 Procedes de preparation d'acides nucleiques pour des analyses de spectrometrie de masse

Publications (1)

Publication Number Publication Date
CA2301875A1 true CA2301875A1 (fr) 1998-03-26

Family

ID=27364108

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002301875A Abandoned CA2301875A1 (fr) 1996-09-19 1997-09-19 Procedes de preparation d'acides nucleiques pour des analyses de spectrometrie de masse

Country Status (3)

Country Link
AU (1) AU4591697A (fr)
CA (1) CA2301875A1 (fr)
WO (1) WO1998012355A1 (fr)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6194144B1 (en) 1993-01-07 2001-02-27 Sequenom, Inc. DNA sequencing by mass spectrometry
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
AU743146B2 (en) * 1997-05-28 2002-01-17 Walter And Eliza Hall Institute Of Medical Research, The Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage
CA2292039A1 (fr) * 1997-05-28 1998-12-03 The Walter And Eliza Hall Institute Of Medical Research Diagnostics d'acide nucleique par spectrometrie de masse ou separation de masse et clivage specifique de la base
US6723564B2 (en) * 1998-05-07 2004-04-20 Sequenom, Inc. IR MALDI mass spectrometry of nucleic acids using liquid matrices
US6074831A (en) * 1998-07-09 2000-06-13 Agilent Technologies, Inc. Partitioning of polymorphic DNAs
WO2000031300A2 (fr) * 1998-11-24 2000-06-02 The Johns Hopkins University Analyse genotypique de fragments d'adn courts en spectrometrie de masse
US6994969B1 (en) 1999-04-30 2006-02-07 Methexis Genomics, N.V. Diagnostic sequencing by a combination of specific cleavage and mass spectrometry
KR20020064298A (ko) * 1999-10-13 2002-08-07 시쿼넘, 인코포레이티드 다형성 유전 마커를 동정하기 위한 데이타베이스 및 이의제조 방법
EP1332000B1 (fr) 2000-10-30 2012-06-20 Sequenom, Inc. Procede d'apport de volumes inferieurs au microlitre sur un substrat
US7226739B2 (en) 2001-03-02 2007-06-05 Isis Pharmaceuticals, Inc Methods for rapid detection and identification of bioagents in epidemiological and forensic investigations
US7666588B2 (en) 2001-03-02 2010-02-23 Ibis Biosciences, Inc. Methods for rapid forensic analysis of mitochondrial DNA and characterization of mitochondrial DNA heteroplasmy
US20040121314A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents in containers
US20040121335A1 (en) 2002-12-06 2004-06-24 Ecker David J. Methods for rapid detection and identification of bioagents associated with host versus graft and graft versus host rejections
US20030027135A1 (en) 2001-03-02 2003-02-06 Ecker David J. Method for rapid detection and identification of bioagents
EP1950305A1 (fr) 2001-05-09 2008-07-30 Monsanto Technology, LLC Gènes tyr et utilisations associées
US7217510B2 (en) 2001-06-26 2007-05-15 Isis Pharmaceuticals, Inc. Methods for providing bacterial bioagent characterizing information
JP2006508632A (ja) * 2002-03-01 2006-03-16 ラブジェン， インコーポレイテッド 遺伝子疾患の検出法
CA2507189C (fr) 2002-11-27 2018-06-12 Sequenom, Inc. Procedes et systemes de detection et d'analyse de variations de sequences bases sur la fragmentation
CA2508726A1 (fr) 2002-12-06 2004-07-22 Isis Pharmaceuticals, Inc. Procedes d'identification rapide de pathogenes chez l'homme et les betes
US7964343B2 (en) 2003-05-13 2011-06-21 Ibis Biosciences, Inc. Method for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
US8158354B2 (en) 2003-05-13 2012-04-17 Ibis Biosciences, Inc. Methods for rapid purification of nucleic acids for subsequent analysis by mass spectrometry by solution capture
WO2005024068A2 (fr) 2003-09-05 2005-03-17 Sequenom, Inc. Analyse de variations de sequences alleles specifiques
US8546082B2 (en) 2003-09-11 2013-10-01 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US8097416B2 (en) 2003-09-11 2012-01-17 Ibis Biosciences, Inc. Methods for identification of sepsis-causing bacteria
US20120122099A1 (en) 2003-09-11 2012-05-17 Rangarajan Sampath Compositions for use in identification of bacteria
US8163895B2 (en) 2003-12-05 2012-04-24 Ibis Biosciences, Inc. Compositions for use in identification of orthopoxviruses
US7666592B2 (en) 2004-02-18 2010-02-23 Ibis Biosciences, Inc. Methods for concurrent identification and quantification of an unknown bioagent
CA2561381C (fr) 2004-03-26 2015-05-12 Sequenom, Inc. Clivage specifique de base de produits d'amplification specifiques de la methylation en combinaison avec une analyse de masse
CA2567839C (fr) 2004-05-24 2011-06-28 Isis Pharmaceuticals, Inc. Spectrometrie de masse a filtration ionique selective par seuillage numerique
US20050266411A1 (en) 2004-05-25 2005-12-01 Hofstadler Steven A Methods for rapid forensic analysis of mitochondrial DNA
US7811753B2 (en) 2004-07-14 2010-10-12 Ibis Biosciences, Inc. Methods for repairing degraded DNA
CA2600184A1 (fr) 2005-03-03 2006-09-08 Isis Pharmaceuticals, Inc. Compositions utilisees pour identifier des virus secondaires
US8084207B2 (en) 2005-03-03 2011-12-27 Ibis Bioscience, Inc. Compositions for use in identification of papillomavirus
US8026084B2 (en) 2005-07-21 2011-09-27 Ibis Biosciences, Inc. Methods for rapid identification and quantitation of nucleic acid variants
DE102006003415A1 (de) * 2006-01-24 2007-08-02 Siemens Ag Verfahren zur Analyse einer Probe
WO2007112289A2 (fr) 2006-03-23 2007-10-04 The Regents Of The University Of California Procédé permettant d'identifier et de séquencer des protéines
EP2010679A2 (fr) 2006-04-06 2009-01-07 Ibis Biosciences, Inc. Compositions pour l'identification de champignons
JP5420412B2 (ja) 2006-09-14 2014-02-19 アイビス バイオサイエンシズ インコーポレイティッド 病原体の同定のための標的全ゲノム増幅方法
JP5680304B2 (ja) 2007-02-23 2015-03-04 アイビス バイオサイエンシズ インコーポレイティッド 迅速な法医学的ｄｎａ分析法
WO2008151023A2 (fr) 2007-06-01 2008-12-11 Ibis Biosciences, Inc. Procédés et compositions pour l'amplification par déplacement multiple d'acides nucléiques
US20090180931A1 (en) 2007-09-17 2009-07-16 Sequenom, Inc. Integrated robotic sample transfer device
EP2344893B1 (fr) 2008-09-16 2014-10-15 Ibis Biosciences, Inc. Systèmes et procédés de manipulation de microplaques
EP2347254A2 (fr) 2008-09-16 2011-07-27 Ibis Biosciences, Inc. Unités de traitement d'échantillons, systèmes et procédés associés
EP2349549B1 (fr) 2008-09-16 2012-07-18 Ibis Biosciences, Inc. Cartouches de mélange, postes de mélange et kits, et système associé
WO2010080616A1 (fr) 2008-12-19 2010-07-15 Abbott Laboratories Dosage moléculaire pour le diagnostic du paludisme
US8158936B2 (en) 2009-02-12 2012-04-17 Ibis Biosciences, Inc. Ionization probe assemblies
WO2010104798A1 (fr) 2009-03-08 2010-09-16 Ibis Biosciences, Inc. Procédés de détection d'un bioagent
US9393564B2 (en) 2009-03-30 2016-07-19 Ibis Biosciences, Inc. Bioagent detection systems, devices, and methods
WO2011008971A1 (fr) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Appareil de levage et de montage
WO2011008972A1 (fr) 2009-07-17 2011-01-20 Ibis Biosciences, Inc. Systèmes pour l'identification d'un bioagent
WO2011014811A1 (fr) 2009-07-31 2011-02-03 Ibis Biosciences, Inc. Amorces de capture et supports solides liés à une séquence de capture pour tests diagnostiques moléculaires
EP3098325A1 (fr) 2009-08-06 2016-11-30 Ibis Biosciences, Inc. Compositions de base déterminée sans masse pour la détection d'acide nucléique
US9890408B2 (en) 2009-10-15 2018-02-13 Ibis Biosciences, Inc. Multiple displacement amplification
US9758840B2 (en) 2010-03-14 2017-09-12 Ibis Biosciences, Inc. Parasite detection via endosymbiont detection
EP2576823A1 (fr) 2010-06-04 2013-04-10 Institut National De La Sante Et De La Recherche Medicale (Inserm) Variants du récepteur à la prolactine constitutionnellement actifs utilisés en tant que marqueurs pronostics et cibles thérapeutiques en vue de la prévention de la progression de cancers hormono-dépendants vers l'hormono-indépendance

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5518900A (en) * 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
GB9122060D0 (en) * 1991-10-17 1991-11-27 Dynal As Method of sequencing double stranded dna
US5547835A (en) * 1993-01-07 1996-08-20 Sequenom, Inc. DNA sequencing by mass spectrometry
EP0886681A1 (fr) * 1996-03-04 1998-12-30 Genetrace Systems, Inc. Methodes de criblage des acides nucleiques par spectrometrie de masse

Also Published As

Publication number Publication date
WO1998012355A1 (fr) 1998-03-26
AU4591697A (en) 1998-04-14

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued