CA2300268A1 - Electrochemical reporter system for detecting analytical immunoassay and molecular biology procedures - Google Patents

Electrochemical reporter system for detecting analytical immunoassay and molecular biology procedures Download PDF

Info

Publication number
CA2300268A1
CA2300268A1 CA002300268A CA2300268A CA2300268A1 CA 2300268 A1 CA2300268 A1 CA 2300268A1 CA 002300268 A CA002300268 A CA 002300268A CA 2300268 A CA2300268 A CA 2300268A CA 2300268 A1 CA2300268 A1 CA 2300268A1
Authority
CA
Canada
Prior art keywords
electrochemical
enzyme
substrate
nucleic acid
reporter system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002300268A
Other languages
English (en)
French (fr)
Inventor
Robert D. Macphee
Clive R. Taylor
Rainer Hintsche
Rene Seitz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fraunhofer Institut fuer Siliziumtechnologie
University of Southern California USC
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US09/105,538 external-priority patent/US6682648B1/en
Application filed by Individual filed Critical Individual
Publication of CA2300268A1 publication Critical patent/CA2300268A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/005—Enzyme electrodes involving specific analytes or enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA002300268A 1997-08-12 1998-08-12 Electrochemical reporter system for detecting analytical immunoassay and molecular biology procedures Abandoned CA2300268A1 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US5546697P 1997-08-12 1997-08-12
US5575997P 1997-08-14 1997-08-14
US10553998A 1998-06-26 1998-06-26
US09/105,538 US6682648B1 (en) 1997-08-12 1998-06-26 Electrochemical reporter system for detecting analytical immunoassay and molecular biology procedures
US09/105,538 1998-06-26
US60/055,759 1998-06-26
US60/055,466 1998-06-26
US09/105,539 1998-06-26
PCT/US1998/016714 WO1999007879A1 (en) 1997-08-12 1998-08-12 Electrochemical reporter system for detecting analytical immunoassay and molecular biology procedures

Publications (1)

Publication Number Publication Date
CA2300268A1 true CA2300268A1 (en) 1999-02-18

Family

ID=27489724

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002300268A Abandoned CA2300268A1 (en) 1997-08-12 1998-08-12 Electrochemical reporter system for detecting analytical immunoassay and molecular biology procedures

Country Status (5)

Country Link
EP (1) EP1003905A1 (ja)
JP (1) JP2001512691A (ja)
AU (1) AU8903998A (ja)
CA (1) CA2300268A1 (ja)
WO (1) WO1999007879A1 (ja)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6358752B1 (en) * 1996-09-27 2002-03-19 Cornell Research Foundation, Inc. Liposome-enhanced test device and method
US6391558B1 (en) 1997-03-18 2002-05-21 Andcare, Inc. Electrochemical detection of nucleic acid sequences
US6682648B1 (en) 1997-08-12 2004-01-27 University Of Southern California Electrochemical reporter system for detecting analytical immunoassay and molecular biology procedures
DE19916921A1 (de) * 1999-04-14 2000-10-19 Fraunhofer Ges Forschung Elektrisches Sensorarray
CA2393733A1 (en) * 1999-12-09 2001-06-14 Motorola, Inc. Methods and compositions relating to electrical detection of nucleic acid reactions
US6518024B2 (en) 1999-12-13 2003-02-11 Motorola, Inc. Electrochemical detection of single base extension
US6824669B1 (en) 2000-02-17 2004-11-30 Motorola, Inc. Protein and peptide sensors using electrical detection methods
FR2805545B1 (fr) * 2000-02-24 2002-05-17 Argene Sa Procede electrochimique de detection d'acides nucleiques
EP1272849A2 (de) 2000-03-30 2003-01-08 Infineon Technologies AG Biosensor, biosensor-array und verfahren zum ermitteln makromolekularer biopolymere mit einem biosensor
US7455975B2 (en) 2000-04-14 2008-11-25 Esa Biosciences, Inc. Electrochemical detection of nucleic acid sequences
US6562209B1 (en) 2001-04-19 2003-05-13 Northrop Grumman Corporation Automated computer controlled reporter device for conducting imunnoassay and molecular biology procedures
US6803202B2 (en) * 2001-10-23 2004-10-12 Northrop Grumman Corporation Combinational strategy for identification of biological agents
EP1376111A1 (en) * 2002-06-24 2004-01-02 Universite Catholique De Louvain Method and device for high sensitivity detection of the presence of DNA and other probes
DE102008025680A1 (de) 2008-05-29 2009-12-03 Siemens Healthcare Diagnostics Gmbh Analyseeinrichtung und Verfahren zum Redoxcycling ohne Potentiostat
DE102008025992B4 (de) 2008-05-30 2011-01-27 Siemens Healthcare Diagnostics Gmbh Titerplatte und Verfahren zur Detektion eines Analyten
DE102008027038A1 (de) * 2008-06-06 2009-12-17 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zum Detektieren von chemischen oder biologischen Species sowie Elektrodenanordnung hierfür
CN109705177A (zh) * 2018-12-24 2019-05-03 郑州安图生物工程股份有限公司 一种底物及其制备方法和应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3479522D1 (en) * 1983-12-16 1989-09-28 Medisense Inc Assay for nucleic acids
US4963245A (en) * 1986-05-02 1990-10-16 Ciba Corning Diagnostics Corp. Unitary multiple electrode sensor
US5391272A (en) * 1992-03-06 1995-02-21 Andcare, Inc. Electrochemical immunoassay methods
DE4318519C2 (de) * 1993-06-03 1996-11-28 Fraunhofer Ges Forschung Elektrochemischer Sensor
DE19541033C1 (de) * 1995-11-03 1997-06-26 Inst Chemo Biosensorik Elektrochemisches Verfahren zur quantitativen Bestimmung von Bindungsproteinen

Also Published As

Publication number Publication date
AU8903998A (en) 1999-03-01
EP1003905A1 (en) 2000-05-31
WO1999007879A1 (en) 1999-02-18
JP2001512691A (ja) 2001-08-28

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued