CA2294525A1 - Expression de la fructose 1,6 bisphosphate aldolase dans des plantes transgeniques - Google Patents

Expression de la fructose 1,6 bisphosphate aldolase dans des plantes transgeniques Download PDF

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Publication number
CA2294525A1
CA2294525A1 CA002294525A CA2294525A CA2294525A1 CA 2294525 A1 CA2294525 A1 CA 2294525A1 CA 002294525 A CA002294525 A CA 002294525A CA 2294525 A CA2294525 A CA 2294525A CA 2294525 A1 CA2294525 A1 CA 2294525A1
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CA
Canada
Prior art keywords
plant
fda
gene
plants
fructose
Prior art date
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Abandoned
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CA002294525A
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English (en)
Inventor
Gerard F. Barry
Nordine Cheikh
Ganesh M. Kishore
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Monsanto Technology LLC
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Individual
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Publication of CA2294525A1 publication Critical patent/CA2294525A1/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La fructose-1,6-bisphosphate aldolase (FDA) est une enzyme destinée à catalyser de manière réversible la réaction transformant le triosephosphate en fructose-1,6-bisphosphate. Cette enzyme est située, à l'intérieur d'une feuille, dans le chloroplaste (synthèse de l'amidon), et dans le cytosol (biosynthèse du sucrose). L'invention concerne la production de plantes transgéniques exprimant le gène E. coli fda dans le chloroplaste, afin d'améliorer le rendement végétal par une augmentation de la production de sucrose en général et un accroissement de l'aptitude de la feuille à la biosynthèse de l'amidon en particulier. Tout en ayant une teneur en sucrose inférieure, les feuilles des plantes exprimant le transgène fda présentent une accumulation de l'amidon considérablement supérieure, en comparaison des plantes témoins exprimant le vecteur nul, en particulier au début de la durée d'éclairement. Ces plantes transgéniques possèdent également une masse de racines sensiblement supérieure. Les pommes de terre transgéniques exprimant le gène fda présentent en outre une uniformité de solides améliorée.
CA002294525A 1997-06-17 1998-06-16 Expression de la fructose 1,6 bisphosphate aldolase dans des plantes transgeniques Abandoned CA2294525A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US4995597P 1997-06-17 1997-06-17
US60/049,955 1997-06-17
PCT/US1998/012447 WO1998058069A1 (fr) 1997-06-17 1998-06-16 Expression de la fructose 1,6 bisphosphate aldolase dans des plantes transgeniques

Publications (1)

Publication Number Publication Date
CA2294525A1 true CA2294525A1 (fr) 1998-12-23

Family

ID=21962657

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002294525A Abandoned CA2294525A1 (fr) 1997-06-17 1998-06-16 Expression de la fructose 1,6 bisphosphate aldolase dans des plantes transgeniques

Country Status (16)

Country Link
EP (1) EP1002114A1 (fr)
JP (1) JP2001520522A (fr)
CN (1) CN1267333A (fr)
AR (1) AR020307A1 (fr)
AU (1) AU735383B2 (fr)
BR (1) BR9810171A (fr)
CA (1) CA2294525A1 (fr)
EA (1) EA200000029A1 (fr)
HU (1) HUP0004228A3 (fr)
IL (1) IL133532A0 (fr)
NO (1) NO996218L (fr)
NZ (1) NZ501781A (fr)
PL (1) PL337581A1 (fr)
TR (1) TR199903157T2 (fr)
WO (1) WO1998058069A1 (fr)
ZA (1) ZA985261B (fr)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1097211B1 (fr) 1998-07-10 2007-04-18 Calgene LLC Expression de peptides eukaryotes dans les plastes de vegetaux
US6512162B2 (en) 1998-07-10 2003-01-28 Calgene Llc Expression of eukaryotic peptides in plant plastids
US7858848B2 (en) 1999-11-17 2010-12-28 Mendel Biotechnology Inc. Transcription factors for increasing yield
US7345217B2 (en) 1998-09-22 2008-03-18 Mendel Biotechnology, Inc. Polynucleotides and polypeptides in plants
US7868229B2 (en) 1999-03-23 2011-01-11 Mendel Biotechnology, Inc. Early flowering in genetically modified plants
JP3357909B2 (ja) * 1999-03-10 2002-12-16 奈良先端科学技術大学院大学長 高等植物の生産性を向上させる方法
MXPA02004884A (es) * 1999-11-17 2004-04-05 Mendel Biotechnology Inc Genes de tolerancia a patogenos.
EP1632576B1 (fr) 1999-12-16 2017-05-31 Monsanto Technology, LLC Nouvelles constructions d'expréssion végétales
AU3089902A (en) * 2000-10-30 2002-05-15 Monsanto Technology Llc Canola event pv-bngt04(rt73) and compositions and methods for detection thereof
US7939715B2 (en) 2000-11-16 2011-05-10 Mendel Biotechnology, Inc. Plants with improved yield and stress tolerance
US8426678B2 (en) 2002-09-18 2013-04-23 Mendel Biotechnology, Inc. Polynucleotides and polypeptides in plants
JP2005130770A (ja) * 2003-10-30 2005-05-26 Ajinomoto Co Inc 植物体あたり得られるデンプン量が増加したバレイショおよびその作出法
EP1774004B1 (fr) * 2004-07-31 2013-07-10 Metanomics GmbH Preparation d'organismes a croissance plus rapide et/ou a meilleur rendement
AU2006314188B2 (en) * 2005-11-16 2014-01-16 Pro Natura Gesellschaft Fur Gesunde Ernahrung Mbh Agent for use in the case of fructose intolerance
MX2008010204A (es) * 2006-02-09 2009-01-27 Japan Science & Tech Agency Planta con capacidad de crecimiento y resistencia a enfermedades mejorada, y metodo para su produccion.
CN102268430B (zh) * 2010-06-02 2013-05-15 中国科学院大连化学物理研究所 果糖-1,6-二磷酸醛缩酶启动子及应用和构建体、载体
WO2017053433A1 (fr) 2015-09-21 2017-03-30 Modern Meadow, Inc. Tissus composites renforcés par des fibres
KR20170096094A (ko) 2016-02-15 2017-08-23 브렌던 패트릭 퍼셀 복합체 물질을 생제작하는 방법
CN105973851B (zh) * 2016-06-12 2018-10-23 中国科学院地球化学研究所 一种定量测定植物光合生长力的方法
CA3008850A1 (fr) 2017-06-29 2018-12-29 Modern Meadow, Inc. Souches de levure et methode de production de collagene
AU2018253595A1 (en) 2017-11-13 2019-05-30 Modern Meadow, Inc. Biofabricated leather articles having zonal properties
US11352497B2 (en) 2019-01-17 2022-06-07 Modern Meadow, Inc. Layered collagen materials and methods of making the same
CN112094859B (zh) * 2020-09-25 2022-01-25 扬州大学 一种凤丹PoFBA基因、表达载体及其制备方法和应用
CN112094851B (zh) * 2020-09-25 2022-01-25 扬州大学 一种凤丹PoCAB151基因、表达载体及其制备方法和应用
CN113528497B (zh) * 2021-07-22 2022-06-24 中国林业科学研究院林业研究所 Fba8基因或fba8蛋白在制备具有磷酸转移活性和/或蛋白水解活性的试剂中的应用
CN113528567B (zh) * 2021-07-22 2022-05-03 中国林业科学研究院林业研究所 Fba8蛋白或由其衍生的蛋白质在调节植物维管束分裂和/或花序轴横截面积中的应用
CN114920938B (zh) * 2022-05-29 2023-11-10 深圳绿天琪生物医药有限公司 精氨酸偶联植物源聚糖与多肽合成共聚物的制备及应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2081885C (fr) * 1990-06-18 2000-10-31 Ganesh M. Kishore Accroissement de la teneur en amidon des vegetaux
DE19502053A1 (de) * 1995-01-13 1996-07-18 Inst Genbiologische Forschung Verfahren und DNA-Moleküle zur Steigerung der Photosyntheserate in Pflanzen, sowie Pflanzenzellen und Pflanzen mit gesteigerter Photosyntheserate
US5716837A (en) * 1995-02-10 1998-02-10 Monsanto Company Expression of sucrose phosphorylase in plants

Also Published As

Publication number Publication date
NO996218D0 (no) 1999-12-15
HUP0004228A2 (en) 2001-03-28
IL133532A0 (en) 2001-04-30
CN1267333A (zh) 2000-09-20
NO996218L (no) 2000-02-14
PL337581A1 (en) 2000-08-28
EP1002114A1 (fr) 2000-05-24
AU735383B2 (en) 2001-07-05
EA200000029A1 (ru) 2000-08-28
JP2001520522A (ja) 2001-10-30
TR199903157T2 (xx) 2000-06-21
NZ501781A (en) 2001-07-27
BR9810171A (pt) 2001-11-06
WO1998058069A1 (fr) 1998-12-23
HUP0004228A3 (en) 2002-11-28
AU8144598A (en) 1999-01-04
AR020307A1 (es) 2002-05-08
ZA985261B (en) 1999-01-07

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FZDE Discontinued

Effective date: 20100616